Your Neuron-Glia Co-Cultures Deserve the Best
There is a lot of cross talk between neurons and glia. In neuro-immuno and neuro-degenerative diseases this cross talk is dysregulated. Robust neuron-glia co-cultures are needed for drug discovery and basic research.Here is a study that references use of co-culturing to study the interactive effects of the viral protein HIV-1 Tat lipopolysaccharide (LPS) on enteric glia and neurons.Here is a study that references use of co-culturing to study the interactive effects of the viral protein HIV-1 Tat lipopolysaccharide (LPS) on enteric glia and neurons. In order to optimize the co-culturing ourMedia, Coatings andGDNF growth fac...
Source: Neuromics - September 19, 2016 Category: Neuroscience Tags: Astrocyte-Glial-Neuron Co-cultures GDNF expression glial cell Glial Fibrillary Acidic Protein Neuronal Differentation Source Type: news
Perky ERKies and Depression
ERK1/2 Signaling and DepressionExtracellular signal-regulated kinase 1/2- (ERK1/2-) mediated cellular signaling plays a major role in synaptic and structural plasticity. Although ERK1/2 signaling has been shown to be involved in stress and depression. Phosphorylation of the receptor is important for maintaining healthy signaling, reduction of oxidative stress and induction of growth factors.We are pleased that our ERK1/2 markers have beenpublished in these type of studies. Here ' s a recent one: MARIA CONCETTA OLIANAS, Simona Dedoni, and PIER LUIGI ONALI. LPA1 mediates antidepressant-induced ERK1/2 signalling and prot...
Source: Neuromics - September 14, 2016 Category: Neuroscience Tags: ERK1/2. depression phospho-ERK1/2 antibody Source Type: news
Multiplexed Protein Array for Immunologists
Assay 1,631 Antibodies in Under 8 Hours!The RayBio ® Immunome Protein Arrays are multiplex protein immunoassays for numerous research applications. Correctly folded, functional proteins are immobilized onto a solid glass slide surface. The slides can then be probed with serum or small molecules and used in protein-antibody studies, small-molecule i nhibition of kinase studies, protein-protein studies, methylation studies or DNA binding studies. Our main immunome array product offering contains 1,631 proteins. Smaller custom arrays are also available.Each 75mm x 25mm glass slide is spotted with 4 identical protein arrays (...
Source: Neuromics - September 4, 2016 Category: Neuroscience Tags: autoimmune disease immune response immune system immunology inflammatory response protein array. Multiplexed immunome protein array Source Type: news
In Depth Analysis of Differentiating Neural Stem Cells
Foundation for Future TherapiesHere we share an example of this analysis. Results from worldwide research is now being made available viahPSCreg:Ilyas Singec, Andrew M. Crain, Junjie Hou, Brian T.D. Tobe, Maria Talantova, Alicia A. Winquist, Kutbuddin S. Doctor, Jennifer Choy, Xiayu Huang, Esther La Monaca, David M. Horn,5 Dieter A. Wolf, Stuart A. Lipton, Gustavo J. Gutierrez, Laurence M. Brill, and Evan Y. Snyder. Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (Phospho)Proteomic Profiling. Stem Cell Reports (2016), http://dx.doi.org/10.1016/j.stemcr.2016.07.01.Images: MDK Expression in P...
Source: Neuromics - August 30, 2016 Category: Neuroscience Tags: Astrocytes Glia Human Neural Progenitors Midkine Neurons Oct4 Antibody OTX-2 antibody Source Type: news
Human Mesenchymal Stem Cells Conjugates
Conclusions:Researchers successfully developed a target-specific systemic delivery system of MSCs to the liver using HA-WGA conjugate. HA-WGA conjugate was synthesized by the coupling reaction of HA-aldehyde with amine group of WGA. GPC analysis revealed the successful synthesis of HA-WGA conjugate. CD analysis corroborated that the secondary structure of WGA remained stable even after conjugation to HA. HA-WGA conjugate appeared to bind to the surface of MSCs and remain stably for up to 1 h. After cell surface modification, most of HA-WGA/MSC complex could be systemically delivered to the liver 4 h after intravenous ...
Source: Neuromics - August 23, 2016 Category: Neuroscience Tags: hMSCs human Mesenchymal Stem Cell Based Assays mesenchymal stem cell conjugates UCB Derived hMSCs Source Type: news
More on Epigenetics and Pain
Great Research Tools!OurMarkers and i-FectTM Transection Kit are used to studyEpigenetics and pain. Here ' s yet another example:M. Leinders, b, N. Üçeyler, R.A. Pritchard, C. Sommer, L.S. Sorkin. Increased miR-132-3p expression is associated with chronic neuropathic pain. Experimental Neurology. Volume 283, Part A, September 2016, Pages 276 –286...The inhibitor and mimetic were administered to awake rats via the it catheters. Prior to injection, active or mismatch inhibitors were mixed with (1:5 w/v) i-Fect™ in vivo transfection reagent(Neuromics, Edina, USA) to final doses of 5, 2 and 1 μg in 10 μl...Fi...
Source: Neuromics - August 19, 2016 Category: Neuroscience Tags: chronic pain iFect miRNA siRNA delivery in-vivo Source Type: news
Protein Purification Solutions
Strep-tag ®Tools + Strep-Tactin ColumnsWe are seeing a notable uptick in use of ourProtein Purification Tools. This includes vectors and purification columns. We are especially delighted with the increased use of of ourStrep-Tag + Strep-Tactin Columns.Figure: Strep-Tag+Strep-Tactin MethodsHere ' s an example of researchers using the technology to purify heme proteins for the study of the diptheria pathogen: Courtni E. Allen and Michael P. Schmitt. Utilization of Host Iron Sources by Corynebacterium diphtheriae: Multiple Hemoglobin-Binding Proteins Are Essential for the Use of Iron from the Hemoglobin-Haptoglobin...
Source: Neuromics - August 13, 2016 Category: Neuroscience Tags: Cloning Vectors CRISP-Cas9 Plasmids Protein Purification Strep-Tag Source Type: news
Growth Factors for Excellent Cultures
Quality CountsCells are the foundation of Cell Based Assays. We provide High Quality Growth Factorsto insure your success. Here ' s a sampling of the categories available:Angiogenic Growth FactorsBone Morphogenetic Proteins (BMPs)Brain Derived Neurotrophic Factors (BDNFs)Ephs/EphrinsEpidermal Growth Factors (EGFs)Fibroblast Growth Factors (FGFs) Hematopoietic Cell Growth Factors Hepatocyte Growth Factors Insulin-Like Growth Factors (IGFs)Neuron-Glial ExpressedPlatelet Derived Growth Factors (PDGFs)Transforming Growth Factors (TGFs)Vascular Endothelial Growth Factors (VEGFs)Images: GFP+ mMN Mouse Mo...
Source: Neuromics - August 8, 2016 Category: Neuroscience Tags: BDNF Brain-Derived Neurotrophic Factor FGF Basic Recombinant Protein Fibroblast Growth Factor Basic GDNF expression Growth factors HGF PDGF proteins TGF-beta VEGF Source Type: news
Large Intestine Endothelial Cells
< b > < i > " Hard to Find " , Potent and Pure < /i > < /b > < br / > < b > < i > < br / > < /i > < /b > We asked one of our Large Biopharma users of our < a href= " http://www.neuromics.com/human-large-intestine-microvascular-endothelial-cells " target= " _blank " > Large Intestine Endothelial cells < /a > , " Why Neuromics " and she told us, " It is hard to find these cells. Let alone ones that work as well as yours! " < br / > < br / > < div class= " separator " style= " clear: both; text-align: center; " > < a href= " https://2.bp.blogspot.com/-ZuBxlAVsqpo/V5susgqRbeI/AAAAAAAADJM/A1Zm6uAx6aYyfiOzf66C9A4co72f84q2QCLc...
Source: Neuromics - July 29, 2016 Category: Neuroscience Tags: endothelial cells human intestinal cells huvecs large intestinal endothelial cells primary human endothelial cells Source Type: news
Human Endothelial Cells and Drug Discovery
< b > < i > Pure, Potent and Culture Ready < /i > < /b > < br / > Alterations of < a href= " http://www.neuromics.com/ittrium/visit/A1x66x1y1x85b1x1x9cy1x622cx1x96y1xb33bx1x8ey1x10d02x1x8ey1x9efex1x82 " target= " _blank " > endothelial cells < /a > and the vasculature play a central role in the pathogenesis of a broad spectrum of the most dreadful of human diseases, as endothelial cells have the key function of participating in the maintenance of patent and functional capillaries. This makes them a key component for Drug Discovery. < br / > < br / > We are proud that Biotech and Biopharma Cos. are increasingly using our ...
Source: Neuromics - July 21, 2016 Category: Neuroscience Tags: HBMECs human brain microvascular endothelial cells Human Endothelial Cells Human Retinal Microvascular Endothelial Cells huvecs MRMECs Source Type: news
Immortalized hMicroglia Now Available
In Stock and Ready to Culture!One of partners needed 10 million hMicroglia to assay TBI related auto-antibodies as key piece of there target discovery program. We were unable to find these cell available on the market. We decided to have them micro-dissected from human brain tissue and then immortalized.This enabled us to answer the bell. We now make them available to you. We are offering 2000 USD off through August 31, 2016...only 2995 USD/1,000,000 immortalized cells.Image: Immortalized Human Microglia in Culture.Cells are cyropreserved and delivered frozen.Questions? e-mail pshuster@neuromics.com or direct phone: 612-80...
Source: Neuromics - July 9, 2016 Category: Neuroscience Tags: hmicroglia Human Microglia Immortlaized human microglia Microglial Cultures Source Type: news
Need Human Cells? Ask Us!
From Astrocytes to Satellite CellsWe have many "hard to find" primary human cells in our catalog. We also have the ability to micro-dissect cells from both healthy and diseased tissue and make the related cells available in about 30 days. Here're are some of the currently available cells.Human Bladder Microvascular Endothelial CellsHuman Cardiac Microvascular Endothelial CellsHuman Dermal Lymphatic Microvascular Endothelial CellsHuman Dermal Microvascular Endothelial CellsHuman Glomerular Microvascular Endothelial CellsHuman Large Intestine Microvascular Endothelial CellsHuman Ovarian Microvascular Endothelial CellsHuman ...
Source: Neuromics - July 7, 2016 Category: Neuroscience Tags: Astrocytes brain microvascular endothelial cells cardiomyocytes Human Endothelial Cells Neurons Schwann Cells Source Type: news
Cells Detachment
Easy, Gentle and CompleteI often get asked if trypsin is the best reagent to detach primary and stem cells. The problem with a trypsin is that it could lyse a percentage of the cells.We recommend our DetachinTM. It offers the following benefits:Gentle and rapid detachment.Maximum cell viability.Effective on a wide range of cells.No mammalian or bacterial byproducts.Stable at 4°C for 2 months.No need to wash detached cells.Here's a recent publication referencing the use of Detachin: Yu Hou, Qi Feng, Miao Xu, Guo-sheng Li, Xue-na Liu, Zi Sheng, Hai Zhou, Ji Ma, Yu Wei,1 Yuan-xin Sun,Ying-yi Yu, Ji-hua Qiu, Lin-lin Shao...
Source: Neuromics - June 28, 2016 Category: Neuroscience Tags: Astrocyte-Glial-Neuron Co-cultures Cell based assays Detachin Neurons stem cell based assays Source Type: news
MeCP2 and Nerve Injury
Conclusions: The study shows a regulatory role for MeCP2 in that changes in global redistribution can result in direct and indirect modulation of gene expression in the DRG. Alterations in genome-wide binding of MeCP2 therefore provide a molecular basis for a better understanding of epigenetic regulation-induced molecular changes underlying nerve injury. (Source: Neuromics)
Source: Neuromics - June 20, 2016 Category: Neuroscience Tags: Delivering siRNA to the CNS epigenetics gene expression analysis i-Fect MECP2 methyl-CpG-binding protein 2 miR-126 Nerve Injury Nerve Sprouting Source Type: news
