Neuroscience Cell Based Markers Applications
Progenitors, Neuronal, Astroglia and PNS Markers A to ZOur Neuron/Synapse, Astrocytes, Glia, Microglia, Olidogodendrocytes, Progenitors and Schwann Cell Markers continue to widely used and frequently published.Here're some recent examples: Cinzia Ambrosi , Cynthia Ren, Gaelle Spagnol, Gabriel Cavin, Angela Cone, Elena E. Grintsevich, Gina E. Sosinsky, Paul L. Sorgen. Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. Published: June 9, 2016. http://dx.doi.org/10.1371/journal.pone.0157073...chicken anti-GFAP in blue (Neuromics, Catalog # CH22102)...Figure 1. Cx43 ...
Source: Neuromics - June 12, 2016 Category: Neuroscience Tags: Astrocyte Markers Glial Fibrillary Acidic Protein Antibody Glial Markers IGF-1; Microglial Markers Neuron Specific Enolase Antibody Neuronal Markers NSE Antibody Source Type: news
Human Astroglia and Schwann Cells-BBB Model
Broadening our CapabilitiesAt the core of our solutions are many options for primary human cells. We are especially pleased that we have growing capabilities to provide new cells to researchers studying autoimmune neuro-degenerative diseases like ALS and MS with the addition of:Schwann CellsHuman Schwann Cells (HSwC) are isolated from human spinal nerve. HSwC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSwC are characterized by immunofluorescence with antibodies specific to S100, GFAP, and CD90. HSwC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yea...
Source: Neuromics - June 3, 2016 Category: Neuroscience Tags: ALS Alzheimer ' s Disease astrocyte cultures Blood Brain Barrier Models human Astrocytes human satellite cells MS Multiple Sclerosis Parkinson Source Type: news
IGF and Mood
Gender Specific?IGF is a known mood modulator. This paper hypothesizes that the impact of increased IGF is sex dependent: Victor Munive, Andrea Santi and Ignacio Torres-Aleman. A Concerted Action Of Estradiol And Insulin Like Growth Factor I Underlies Sex Differences In Mood Regulation By Exercise. Scientific Reports 6, Article number: 25969 (2016) doi:10.1038/srep25969.Our IGF-I R/CD221-Fluorescein Labeled was used to link Estrodial with elevating IGF-I levels in female mice.Images: (A) Cultured brain endothelial cells accumulate significantly more IGF-I after treatment with 10−10M estradiol (E2). Representative blot is...
Source: Neuromics - May 18, 2016 Category: Neuroscience Tags: IGF IGF-RI Insulin Growth Factor Mood Source Type: news
Cancer-Associated Fibroblasts and Cancer Cell Migration
Highway for Cancer MetastasisCancer Associated Fibroblasts provide the Matrices (ECMs) serve as cancer’s assistants as it spreads throughout the body.Cancer cells (red) migrate on a CAF-derived extracellular matrix (green). VANDERBILT UNIVERSITY; BEGUM ERDOGAN, DONNA WEBBUnlike other ECMs, which normally form dense meshes, these arrange into parallel bundles forming the highway for tumor cell migration (The Scientist-December 15, 2015). (Source: Neuromics)
Source: Neuromics - May 13, 2016 Category: Neuroscience Tags: CAFs cancer Cancer Associated Fibroblasts cancer biology cancer-associated fibroblasts cell migration metastasis Source Type: news
New Neuronal Astroglial PNS Markers
Save 70 USD on High Titer AntibodiesThe foundation of our company is built on our catalog of proven, published and high titer Neuron-Astroglia and PNS Markers. We are pleased to offer 75 USD off our latest additions. Antibody Type Species Reactivity Applications Aldolase-C Calbindin Calretinin Fox3/NeuN MAP2 Myelin Basic Protein Neurofilament NF-H Neurofilament NF-M Parvalbumin Secretagogin VSNL1 Mouse IgG Chicken IgY Chicken IgY Mouse IgG Mouse IgG ...
Source: Neuromics - May 2, 2016 Category: Neuroscience Tags: Astrocyte marker antibody Astrocyte Markers Astrocyte-Glial-Neuron Co-cultures glial cell Glial Markers Muscle Nerve junction neural markers Neuronal Markers Source Type: news
ELISA Blocking Buffers
Paramount to Reducing Background Noise and Attaining an Accurate SignalOnce the antibody or protein antigens have been properly adsorbed onto the ELISA/EIA plate, the next critical step in creating a reliable immunoassay is the blocking of the plate. This key is:Preventing nonspecific binding Reducing ELISA background signal Blocking nonspecific binding to adsorbed proteins Stabilizing proteins adsorbed to plate for better interactions Neuromics's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing th...
Source: Neuromics - April 26, 2016 Category: Neuroscience Tags: Angiogenesis Antibody Arrays ELISA Blocking Buffers ELISA Techniques sandwich ELISA Source Type: news
Isolating and Selecting T-Cells
Save 100 USD on New KitsWe are pleased to announce the addition of our New T-Cell Isolation/Selection Kits. They are fast and easy. Check out the Short Protocol.Figure CD4+ T-Cell Isolation Process doi:10.1371/journal.pone.0035798.We also have T-Cell Expansion Kits!NameCatalog #TypeSpeciesApplicationsSizePriceStrepMan Magnet for 15ml and 50ml Tubes6-5650-065Cell Isolation-SeparationHCell Assays1 Magnet$400CD3 Fab Streptamer Isolation Kit MB6-8000-201Cell Isolation-SeparationH1 Kit$699CD4 Fab Streptamer Isolation Kit MB6-8000-206Cell Isolation-SeparationH1 Kit$699CD8 Fab Streptamer Isolation Kit MB6-8000-203Cell Isolation-S...
Source: Neuromics - April 15, 2016 Category: Neuroscience Tags: Cancer Research CD4 Cells CD8 Cells immunology T-Cell Expansion T-Cell Isolation Source Type: news
Magic Red and Cancer Research
Measure Apoptosis in Real Time in Cancer CellsMagic Red™ (MR) Kits measure apoptosis via active caspases and cathepsins in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases.In this example, Magic Red is used to measure apoptosis in HeLa Cells after treatment with Artesunate (ART), an anti-malarial drug and potential chemotherapy agent. Figure: ART activates lysosomal function. A, HeLa cells were first treated with ART (50 μM) for 12 h, followed by LTR labeling for 30 min, and further analyzed using confocal microscopy. B, HeLa ce...
Source: Neuromics - April 2, 2016 Category: Neuroscience Tags: chemotherapies chemotherapy HeLA Cells live cell imaging Magic Red Magic Red Apoptosis Kit Source Type: news
Human Astrocytes
Cortex Derived AstrocytesNeuromics is pleased to be offering yet another option for culturing Human Astrocytes. They can be passaged up to 10X-Only 749 USD/500,000 CellsThey are deigned to be easy to culture and grow. Image: Human Brain Astrocytes cultured with our AlphaBioCoat.Check out our large offering of Neurons, Astroglia, Progenitors, Brain Endothelial Cells/Pericytes and Blood Brain Barrier (BBB) Model. Should you have questions on these or any of our offerings, I can be reached directly at 612-801-1007 or pshuster@neuromics.com. Thank you, Pete Shuster-CEO and Owner. (Source: Neuromics)
Source: Neuromics - March 31, 2016 Category: Neuroscience Tags: BBB Model Blood Brain Barrier Models human Astrocytes human brain microvascular endothelial cells Human Neural Progenitors Neurons Pericytes Source Type: news
Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: Neuromics - March 24, 2016 Category: Neuroscience Tags: DOR Dorsal Root Ganglia Ga9 MOR inhibitor i-Fect Morphine Mu Opioid Receptor Neuropathic Pain Source Type: news
3-D Tissue Models Made Easy
3 Steps to an in vivo Like AssayWe provide both CollaGel Hydrogels with different stiffness options and ready made 3-D Human Tissue Models. Building these models can be done in 3 easy steps:Step 1: Thaw our CollaGel Hydrogel solution Step 2: Add your cell suspensions Step 3: Watch it gelHUVECS intergrated into 3-D CollaGel environment.Due to the way in which it gels, our Collagel Hydrogel is well suited to Bio Printing models.I will be posting more data as it becomes available. (Source: Neuromics)
Source: Neuromics - March 15, 2016 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Tissue Modles 3D Cell Based Assays. 3D collagen scaffolds Collagel human umbilical cord endothelial cells Source Type: news
CRISPR Everywhere
Nature Explores What it MeansHere's the link: http://www.nature.com/news/crispr-everywhere-1.19511We are part of the CRISPR everywhere revolution!Features Include:Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual Potent-100% Stable with No Off target effects with the safest transposon insertion profile. Fast- Transfect with our transposase + Sleeping Beauty TransposonTM Reporter Series Vectors. Cost Effective- Starting at $329 (Source: Neuromics)
Source: Neuromics - March 12, 2016 Category: Neuroscience Tags: CRISPR CRISPR-Cas9 gene editing Plasmid transposase transposons Source Type: news
CRISP Everywhere
Nature Explores What it MeansHere's the link: http://www.nature.com/news/crispr-everywhere-1.19511We are part of the CRISPR everywhere revolution!Features Include:Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual Potent-100% Stable with No Off target effects with the safest transposon insertion profile. Fast- Transfect with our transposase + Sleeping Beauty TransposonTM Reporter Series Vectors. Cost Effective- Starting at $329 (Source: Neuromics)
Source: Neuromics - March 12, 2016 Category: Neuroscience Tags: CRISPR CRISPR-Cas9 gene editing Plasmid transposase transposons Source Type: news
Check Out Our 3D Human Skin Tissue Model
In vivo Like Model for Studying Skin Diseases/IrritantsI am pleased to announce this new addition to our Cell Based Assay offerings.3D Human Skin Tissue ModelCross section of Human Skin ModelThe model includes precultured tissue in our engineered and tissue/cell specific Collagel-Hydrogels.Our 3D human skin model is manufactured in a GMP lab. The model is engineered from multilayered, differentiated epidermal cells. The tissue is provided cultured in inserts using serum free media. The model mimics human skin. It provides an in vivo like environment for studying dermal related diseases and cancers. It is also excellent fo...
Source: Neuromics - March 4, 2016 Category: Neuroscience Tags: 3-D Angiogenesis Model 3-D Cell Culturing 3D Cell Based Assays. 3D collagen scaffolds 3D Human Skin Tissue Model BBB Model Blood Brain Barrier Models Skin Cancer Source Type: news
Stem Cell " Stemness " and Differentiation States
< b > < i > Markers Play a Key Role < /i > < /b > < br / > < b > < i > < br / > < /i > < /b > Our < a href= " http://www.neuromics.com/ittrium/visit/A1x66x1y1x85b1x1x9cy1x6217x1x96y1x581x1x82 " target= " _blank " > Stem Cell Markers < /a > are widely used and frequently published. These play an important role for understanding the molecular events that take place during the differentiation of human pluripotent cells. They are essential for the protocols aimed at insuring the generation high of quality differentiated cells. < br / > < br / > Here ' s an excellent study on the determining the state of differentiating stem ...
Source: Neuromics - February 22, 2016 Category: Neuroscience Tags: adult stem cells embryonic stem cells Stem Cell Differentiation Stem Cell Differentiation Protocol Source Type: news
