Stem Cell "Stemness" and Differentiation States
Markers Play a Key RoleOur Stem Cell Markers are widely used and frequently published. These play an important role for understanding the molecular events that take place during the differentiation of human pluripotent cells. They are essential for the protocols aimed at insuring the generation high quality differentiated cells.Here's an excellent study on the determining the state of differentiating stem cells: Julio Castaño, Cristina Morera, Borja Sesé, Stephanie Boue, Carles Bonet-Costa, Merce Martí, Alicia Roque, Albert Jordan, Maria J. Barrero. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. Publ...
Source: Neuromics - February 22, 2016 Category: Neuroscience Tags: adult stem cells embryonic stem cells Stem Cell Differentiation Stem Cell Differentiation Protocol Source Type: news
3-D Cell Based Assays Are Evolving
3-D Multicell Models and GelsThere is a growing body of publications of why 3-D matters in drug discovery and toxicology assays. This table shows important distinctions for single cell assays. Cellular characteristics 2D 3D Refs. Morphology Sheet-like flat and stretched cells in monolayer Natural shape in spheroid/aggregate structures 20,24,50 Proliferation Often proliferate at a faster rate than in vivo May proliferate at a faster/slower rate compared to 2D-cultured cells depending on cell type and/or type of 3D model system 17,51 Exposure to medium/drugs Cells in mon...
Source: Neuromics - February 15, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell based Assays Astrocytes BBB Model Blood Brain Barrier Models brain microvascular endothelial cells Pericytes Source Type: news
Ready to Use Blood Brain Barrier Model (BBB)
6, 12 and 24 Well OptionsWe are pleased to introduce our BBB Model. It is designed to provide more in vivo like data for your drug discovery and toxicology assays.Our model mimic transport properties of the BBB due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. The model includes brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of sec...
Source: Neuromics - February 9, 2016 Category: Neuroscience Tags: 3-D collagen scaffolds 3D Cell Based Assays. BBB < Blood Brain Barrier Model drug permeability assays human Astrocytes human brain microvascular endothelial cells Pericytes Source Type: news
More and More OR Pubs
Potent, Proven and Frequently PublishedMany labs have used our Opioid Receptor antibodies over the past 10+ years. We are grateful for the many publications and testimonals referencing successful use of these antibodies.Here's a sampling of more recent publications: Wendy M. Walwyn, Wenling Chen, Hyeyoung Kim, Ani Minasyan, Helena S. Ennes, James A. McRoberts, and Juan Carlos G. Marvizón. Sustained Suppression of Hyperalgesia during Latent Sensitization by μ-, δ-, and κ-opioid receptors and α2A Adrenergic Receptors: Role of Constitutive Activity. The Journal of Neuroscience, 6 January 2016, 36(1): 204-221; d...
Source: Neuromics - January 25, 2016 Category: Neuroscience Tags: DOR Antibody Kappa Opioid Receptor Antibodies KOR Antibody MOR Antibody Mu Opioid Receptor antibody Opioid Receptors Antibodies Source Type: news
Why You Should Consider Culturing Your Cells in 3-D
2-D versus 3-D and meaningful outcomesWe have seen recent growth in our customers' demand for 3-D cell based assay solutions. This could be due to more the fact that these assays our proving to generate more in vivo like data.Breast cancer cells, for example, grown in 2-D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3-D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Ano...
Source: Neuromics - January 14, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds 3-D nanofibers breast cancer Source Type: news
Why You Should Consider Culturing Your Cell in 3-D
2-D versus 3-D and meaningful outcomesWe have seen recent growth in our customers' demand for 3-D cell based assay solutions. This could be due to more the fact that these assays our proving to generate more in vivo like data.Breast cancer cells, for example, grown in 2-D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3-D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Ano...
Source: Neuromics - January 14, 2016 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds 3-D nanofibers breast cancer Source Type: news
Sleeping Beauty (SB) Transposon for the Study of Cancers
In this study, a modified SB delivery system, under control of the hTERT promoter in conjunction with the SV40 enhancer, was able to successfully transfer the suicide gene (HSV-TK) into multiple types of cancer cells. The modified SB transfected cancer cells exhibited a significantly increased cancer cell specific death rate. These data suggest that our modified SB-based gene delivery system can be used as a safe and efficient tool for cancer cell specific therapeutic gene transfer and stable long-term expression."Figure 8. The effect of modified SB system on the tumor growth in vivo.Lung cancer cells (H358) (A), prostate ...
Source: Neuromics - January 9, 2016 Category: Neuroscience Tags: cancer cell cultures Cancer Research Custom Transposons gene expression analysis gene integration sleeping beauty Source Type: news
CRISPR-Cas9 and Sleeping Beauty Transposons
Neuromics-B-Mogen ApproachThe CRISPR-Cas9 gene editing approach is now officially considered a revolution. This is confirmed by the increasing frequency of this word being used across the web in describing this technique. In fact, it is now appearing in conference titles. see: Genome Engineering: The CRISPR/Cas Revolution 2016COLD SPRING HARBOR - 17 AUG 2016.We have joined the revolution with our new Sleeping Beauty TransposonTM Systems.Figure: Sleeping Beauty TransposonTM Systems Gene Integration Process. (A) A depiction of Sleeping Beauty transposon plasmid and Sleeping Beauty transposase enzyme active in cellular nuclei...
Source: Neuromics - January 3, 2016 Category: Neuroscience Tags: cas9 CRISP CRISP-Cas9 gene editing gene expression analysis gene integration Sleeping Beauty. transposase transposons Source Type: news
Fibroblasts into Neurons
Via Activation of Oct4We have a variety of stem cell, progenitor and neuron markers that are often referenced in publication. There represent a qualitative way to determine the state of stem cells as they differentiate.Here're researchers take Fibroblasts and differentiate them into various progenitors. They were able to further differentiate these progenitors into astrocytes and neurons by: For neuronal differentiation, after 2 days of incubation with 20 nM reversine, cells were then treated with 0.5 μM all-trans-retinoic acid (RA) in serum-free DMEM/F-12 medium supplemented with the ITS for 2 days and switched into ser...
Source: Neuromics - December 17, 2015 Category: Neuroscience Tags: fibroblasts GFAP Antibody Glial Fibrillary Acidic Protein Antibody neural progenitors Neuron Specific Enolase Antibody NSE Antibody Source Type: news
Kv Channels and Pain Transmissiom
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. s...
Source: Neuromics - December 9, 2015 Category: Neuroscience Tags: i-Fect KV receptors. i-Fect siRNA delivery in-vivo Source Type: news
Neuron Astrocyte Glial Markers
Potent and Frequently Published!We have a stout catalog of Neuron-Astrocyte and Glial Markers.They are widely used and frequently published. Here're some recent examples:Mouse Monoclonal GFAP: Chelsea M. Larabee, Constantin Georgescu, Jonathan D. Wren and Scott M. Plafke. Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neuroscience201516:76 DOI: 10.1186/s12868-015-0194-y© Larabee et al. 2015.Image: Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L (green). The GFAP antibody...
Source: Neuromics - November 28, 2015 Category: Neuroscience Tags: Astrocyte-Glial-Neuron Co-cultures GFAP Antibody Immunfluorescence immunoblotting Immunohistochemistry Neurofilament Markers Neuron Markers Source Type: news
Staining Neurons and 3-D
In this study researchers use our Tuj-1/Beta Tubulin Antibody to stain neurons in 3-D Cultures: Gaoying Sun, Wenwen Liu, Zhaomin Fan, Daogong Zhang, Yuechen Han, Lei Xu1, Jieyu Qi, Shasha Zhang, Bradley T. Gao, Xiaohui Bai,Jianfeng Li,Renjie Chai, Haibo Wang. The three-dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro...Tuj-1-β-tubulin (1:1000, Neuromics, USA)... Images: Morphology of the SGNs growth cone cultured in 2D and 3D systems. Phalloidin, green; β-tubulin, red.I will continue to post positive developments concerning use of ou...
Source: Neuromics - November 20, 2015 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Cell Culturing Immunofluorescence Neurons Tuj 1 (Neuron-specific class III beta-tubulin) antibody Tuj 1 antibody Tuj-1 antibodies Source Type: news
Reconstructing 3D Living Biomaterials
In this study, researchers use our Collagen Hydrogel, Collagen Hydrogel Soft and Collagen Hydrogel Soft+ to describe in vivo applications using a murine model to interrogate biocompatibility and cellular behavior post-transfer.Luc macrophages (CC or BES) were then incorporated into a fl uid mixture containing one of three alternative collagen-based hydrogels, namely, Collagen Hydrogel, Collagen Hydrogel Soft, or Collagen Hydrogel Soft+ (resulting in six possible combinations of macrophages and biopolymer).Overview of ResultsFigure: Bioluminescent imaging of implanted macrophage–biopolymer mixes. IC-21-L...
Source: Neuromics - November 11, 2015 Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Cell based Assays 3-D Cell Culturing 3-D collagen scaffolds Hydrogel regenerative medicine tissue engineering tissue regeneration tissue repair Source Type: news
Human alpha-Sensory Neurons
Put Them to Work!We continue to add unique primary Human Neurons to our offerings.We have discounted our new alpha-Sensory Neurons to make it easier to buy and try-only 850 USD/1,000,000 cells.Just thaw and culture. Simply culture on ECM- Laminin or Fibronectin coatings with our alpha Motor Neuron Maintaining Media. Questions? Please contact me directly 612-801-1007 or pshuster@neuromics.com. Pete Shuster, CEO and Owner, Neuromics. (Source: Neuromics)
Source: Neuromics - November 7, 2015 Category: Neuroscience Tags: Human Primary Neurons human sensory neurons. SNs neuronal cultures primary human cells Source Type: news
Solutions for Blood Brain Barrier Research
Neuromics Has the Key Components!We are pleased to add Human Brain Microvascular Pericytes (HBMVPCs) to our solution set for Blood Brain Barrier (BBB) Researchers. These join our Human Brain Microvascular Endothelial Cells and hAstroPro Human Astrocyte+Astroglial-Neurons Co-Culturing Kits to round out our offerings of cells involved in BBB regulation. Image: Human Brain Pericytes cultured with AlphaBioCoat (AC001).Pericytes function at the BBB in at least two ways: by regulating BBB-specific gene expression patterns in endothelial cells, and by inducing polarization of astrocyte end-feet surrounding CNS blood vessels ...
Source: Neuromics - October 29, 2015 Category: Neuroscience Tags: BBB Blood Brain Barrier Blood Brain Barrier Models brain microvascular endothelial cells human brain microvascular cells human brain microvascular endothelial cells Source Type: news
