Autism Spectrum Disorder (ASD) Custom Array Results
Blood Serum Levels and Key MarkersWe have been testing immune/inflammatory response, oxidative stress and growth factor markers in ASD children from central Europe. Most showed high levels of  related cytokines and chemokines.From our initial testing and published results, we have developed a custom Quantibody Array to test ASD children. The markers in this array are: BDNF, HSP-70, Leptin, RAGE and TGF-beta1.Figure: Serum Levels of Key Markers in Tested ASD ChildrenHere's more on each marker:BDNF is a protein involved in making healthy new neurons. This protein is dis-regulated in autism. It is shown to be decreased i...
Source: Neuromics - May 11, 2014 Category: Neuroscience Tags: autism spectrum disorder Autistic Autsim BDNF Heavy metals HSP70 immune response inflammatory response neuroinflammatory cytokines Quantibody Arrays RAGE Source Type: news

Pancreatic Cancer Tumor Cell Assays
Providing Pancreatic Tumor Cells or Related Custom AssaysWe are pleased to announce the addition of Pancreatic Tumor Cells to our Cell Based Assay offerings. We are also generating a wealth of internal data with our Tumor Cells and Cancer Associated Fibroblasts. We have now opened up our capabilities to Cancer Researchers via our new CRO services. These included customized assays for studying toxicity using compounds/small molecules. Here's an example:In addition, we can do kinetic, live cell screens using these cells plus our hMSC and CAFs. To learn more call 612 -801-1007 e-mail me: pshuster@neuromics.com. We stand ready...
Source: Neuromics - May 2, 2014 Category: Neuroscience Tags: CAFs Cancer Associated Fibroblasts live cell imaging Live cell screening pancreas cancer pancreatic cancer Pancreatic tumor cells Source Type: news

Extracellular Matrix Environment and Chemotherapeutics
We report that the efficacy of a Raf kinase inhibitor, sorafenib, is reduced on stiff, collagen-rich microenvironments, independent of ROCK activity. Instead, sustained activation of JNK mediated this resistance, and combining a JNK inhibitor with sorafenib eliminated stiffness-mediated resistance in triple negative breast cancer cells. Surprisingly, neither ERK nor p38 appears to mediate sorafenib resistance, and instead, either ERK or p38 inhibition rescued sorafenib resistance during JNK inhibition, suggesting negative crosstalk between these signaling pathways on stiff, collagen-rich environments. Overall, we discovere...
Source: Neuromics - April 19, 2014 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Cell Culturing chemotherapies chemotherapy Collagen Drug Discovery Extracellular Matrix Highthroughput Screening Source Type: news

HIV-1R Viral Protein R and Memory Impairment
Using Synpatophysin as Marker for Synaptic LossOur Neuron-Glial Markers continue to shine in challenging applications. Here researchers examined whether infusion of the Vpr-expressing astrocytes affected synaptophysin expression in the hippocampus. The authors of the study, using Neuromics' Mouse Monoclonal Synaptophysin Antibody,  found a significant reduction in synaptophysin staining in CA3: Lilith Torres and Richard J Noel. Astrocytic expression of HIV-1 viral protein R in the hippocampus causes chromatolysis, synaptic loss and memory impairment. Journal of Neuroinflammation 2014, 11:53 doi:10.1186/1742-2094-11-53...
Source: Neuromics - April 18, 2014 Category: Neuroscience Tags: Astrocyte Markers HIV Neuron Markers Synaptic Loss Synaptic Markers Synaptophysin Antibody Source Type: news

SOX that Rocks
Excellent Adult, iPSC and eSC Markers Our SOX Markers consistently deliver in helping determine differentiation states of Stem Cells.Here are some recent pubs referencing use of these important antibodies:Xiaoling Wang, Yingjia Wang, He Huang, Buyuan Chen, Xinji Chen, Jianda Hu, Tammy Chang, Ren-Jang Lin, Jiing-Kuan Yee Precise Gene Modification Mediated by TALEN and Single-Stranded Oligodeoxynucleotides in Human Cells. Published: April 01, 2014DOI: 10.1371/journal.pone.0093575... Sox2 (1:100, Neuromics, Minneapolis, MN)... Megumi Kowno, Kanako Watanabe-Susaki, Hisako Ishimine, Shinji Komazaki, Kei Enomoto, Yasuhiro S...
Source: Neuromics - April 15, 2014 Category: Neuroscience Tags: SOX17 antibody sox2 antibody Stem cell markers Source Type: news

Osteoblast Activators and Musculoskeletal Disease Assays
We now have the ability to precisely test human Osteoblast activating agents. Tools available include: Live cell screeningQuantibody Bone Metabolism ArraysPCRFlow CytometryImmunostainingWestern BlottingThis means we can generate data that provides you a clear picture of the effects of small molecules/compounds on the activation, expansion and migration of Osteoblasts. Here's an example.Image: Activin A expression from Osteoblasts treated with Activators vs ControlsWe will soon be releasing a turn-key assay for Osteoporosis Drug Discovery. We can also run custom assays that fit your specific requirements. To learn more...
Source: Neuromics - April 4, 2014 Category: Neuroscience Tags: Activin A Bone Metabolism human osteobalsts live cell imaging Live cell screening Musculoskeletal Disease Osteoporosis Source Type: news

hN2 Primary Human Neurons and Toxicity Assays
Neuromics' hN2 Cells Continue to ShineI previously posted our Human hN2 Neurons being used for studying the mechanisms of  Nerve Agent VX: hN2 Human Neurons for Toxicity Screening. The versatility of these neurons enabled the researchers to perform a microarray study in which cultured human neural cells were exposed to 0.1 or 10 μM of VX for 1 h. Global gene expression changes were analyzed 6, 24, and 72 h post exposure. Solid primary cell based assay results start with healthy and well behaving cells.I am pleased to announce success in exposing the cells to a specific cytotoxicity inducing comp...
Source: Neuromics - March 27, 2014 Category: Neuroscience Tags: Cell Based Assays. Cytotoxicity Assays Dose-Reponse hN2 Neurons Cell Cultures live cell imaging Neurotoxicity Primary Human Neurons Source Type: news

Autism, Inflammation and Stem Cell Enhancers
Proving the Therapeutic ValueWe have been running Quantibody® Antibody Arrays on blood serum of children diagnosed with Autism Spectrum Disorder (ASD). All reside in areas of heavy industry in Central Europe. All have elevated levels of one or several heavy metals.These assays are being run as part of our strategy of treating these children with natural stem cell enhancing supplements. Here are the average serum levels of 2 cytokines (IL-6 and TNF-alpha) and 1 related chemokine (CCL3). All of the children had elevated levels vs healthy controls:Figure: Serum ASD levels vs Healthy Controls (pg/ml)TNF-alpha and IL-6 prom...
Source: Neuromics - March 23, 2014 Category: Neuroscience Tags: ASD Autism autism spectrum disorder Chemokines. Cytokines hMSCs hN2 Neurons Cell Cultures Human Mesenchymal Stem Cells Human Neurons Source Type: news

Irritable Bowel Syndrome (IBS) and BDNF
In this study, Researchers used Neuromics'  i-FectTM Transfection Kit to deliver BDNF to determine effect on visceral hypersensitivity (VHS): J. H. Winston1 Q. Li1, S. K. Sarna1. Chronic prenatal stress epigenetically modifies spinal cord BDNF expression to induce sex-specific visceral hypersensitivity in offspring. Article first published online: 4 MAR 2014. Neurogastroenterology & Motility. DOI: 10.1111/nmo.12326. The Journal of Pain, Volume 14, Issue 11, November 2013, Pages 1485–1491 http://dx.doi.org/10.1016/j.jpain.2013.07.007 Intrathecal treatment with brain-derived neurotrophic factor (BDNF) ant...
Source: Neuromics - March 20, 2014 Category: Neuroscience Tags: BDNF i-Fect Source Type: news

Autism, Heavy Metals, BDNF and HSP-70
Autism Spectrum Disorder (ASD) Testing using Custom Quantibody® ArrayWe recently completed testing blood serum from ASD children. These children were all from Central Europe and lived in cities with heavy industries. All had elevated levels of heavy metals like Aluminum and Copper. We believe that these levels may be contributing to the pathogenesis of the ASD symptoms.Though the sample was small, the results were striking. The elevated levels of Heat Shock Protein 70 (HSP 70) is consistent with high heavy metal toxicity. Studies have reported both elevated and decreased levels of Brian Derived Neurotrophic Factor (BDN...
Source: Neuromics - February 28, 2014 Category: Neuroscience Tags: ASD Autism Autism Bio-markers autism spectrum disorder Autistic BDNF Heat Shock Proteins Quantibody Arrays Source Type: news

Kinetic Assays and Stem Cell Toxicology Studies
UCB Derived hMSCs and CobaltHere're results from a recent Kinetic Assay study we conducted using our Umbilical Cord Blood Derived Stem Cells:Images and Figure: Images: Dose-Response curve for Co++ toxicity to Hoechst-stained hMSCs (UCB Derived catalog number SC00A1-HC). The bar graph on the lower right shows cell counts verses [Co++] at 24, 48 & 72 hours exposure to Co++. Results at 144 hours showed massive cell death. The initial increase in cell counts at low concentration may reflect the well-known activation of HIFs by Co++. Counts were determined by Hoechst-stained MSCs and simultaneous propidium iodide staining s...
Source: Neuromics - February 26, 2014 Category: Neuroscience Tags: Cobalt Drug Discovery hMCS hMSCs human Mesenchymal Stem Cell Based Assays Stem cell cutlures Umbilical Cord Blood. Kinetic Cell Based Assays Source Type: news

More MOR Publications
Latest Mu Opioid PublicationsOur Mu Opioid Receptor Antibodies have set the standard for the study of Pain Mechanisms. We have posted>40 publications referencing use of these antibodies.Here's several published in 2014:Charlie H.T. Kwok, Ian M. Devonshire, Andrew J. Bennett, Gareth J. Hathway. Postnatal maturation of endogenous opioid systems within the periaqueductal grey and spinal dorsal horn of the rat. PAIN - January 2014 (Vol. 155, Issue 1, Pages 168-178, DOI: 10.1016/j.pain.2013.09.0220. ...rabbit anti-MOR (Neuromics, Edina, MN, USA; 1:1000 with tyramide signal amplification protocol)... Images: Immunohistochemi...
Source: Neuromics - February 20, 2014 Category: Neuroscience Tags: chronic pain Inflammatory pain MOR antibodies MOR Antibody Mu Opioid Receptor Antibodies Mu Opioid Receptor antibody MuOpioid Receptors Antibodies Nociception Pain Research Source Type: news

Quantibody Arrays for Tracking Patient Health
Neuromics is working with Dr. Joe Smarda, a renowned Immunologist, to track levels of cytokines in the blood serum of his clients. We have selected RayBiotech's Quantibody® Arrays for these assays. The Clinics in Joe's network treat his clients for autoimmune related disorders.Our regime is:Test clients pre-treatmentTreatTestRefine treatmentTest The specified treatment regime is continued until clients have blood serum cytokine levels that are in the range of our healthy controls. Here's data from our Quantibody® T-helper cell Cytokine Arrays (pre-treatment).Figures IL-6, IL-1 beta, MCP-1 and PAI1 Array r...
Source: Neuromics - February 10, 2014 Category: Neuroscience Tags: autoimmune disease. Rheumatoid Arthritis immune response inflammatory response Quantibody Arrays Raybiotech Source Type: news

Pot and Pain
The pressure for states to legalize Marijuana for medical and recreational use is building. The tax benefits are self evident.The debate for many centers of "true medical benefits". That's why research on understanding analgesic pathways is so important. Ironically, this study was conducted by my friends at Université de Montréal and Université de Sherbrooke in Quebec Canada: J. Desroches, J.-F. Bouchard, L. Gendron, P. Beaulieu. Involvement of cannabinoid receptors in peripheral and spinal morphine analgesia ☆ Neuroscience, Volume 261, 7 March 2014, Pages 23–42. http://dx.doi.org/10....
Source: Neuromics - January 28, 2014 Category: Neuroscience Tags: Cannabinoid Type-1 Receptors Cannabinoid Type-2 Receptors chronic pain Marijuana Mu Opioid Receptor antibody Nociceptive Pain Opioid Receptors Source Type: news

FGF and Stem Cells-Options Matter
Proven, Potent and Cost Effective Fibroblast Growth Factors (FGF).Neuromics have a wealth of expertise in Stem Cells, Media and Growth Factors. FGF is an important component of Stem Cell Based Assays. Our goal is to provide an FGF that fits your requirements like "hand in glove".Here's a small sampling:ISO-kine bFGF-100% animal free and serum free-is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.Images A: Exp...
Source: Neuromics - January 21, 2014 Category: Neuroscience Tags: Cell Cultures FGF Basic FGF Basic Recombinant Protein FGF2 Fibroblast Growth Factor 2 Human Mesenchymal Stem Cells Stem Cell Research Source Type: news

TRPV1 and Osteoarthritis Related Pain
Our TRPV Antibodies are widely used and frequently published. Many of these feature TRPVs' role in nociceptive pain. Specifically they play important roles in the detection of noxious stimuli and inflammatory hyperalgesia.TRPV1 has been implicated in OA pain, both in animal models and by the finding that TRPV1 genetic variants are associated with the risk of symptomatic knee OA in humans: S Kelly, R J Chapman, S Woodhams, D R Sagar, J Turner, J J Burston, C Bullock, K Paton, J Huang, A Wong, D F McWilliams, B N Okine, D A Barrett, G J Hathway, D A Walsh, V Chapman. Increased function of pronociceptive TRPV1 at the level of...
Source: Neuromics - January 13, 2014 Category: Neuroscience Tags: inflammatory nociception Osteoarthritis TRPV1 antibodies TRPV1s Vanilloid Receptor Antibodies VR1-C antibody Source Type: news

Autism Spectrum Disorder and Biomarkers
We used our IFN-gamma and BDNF Sandwich Elisa Kits to test the levels of these biomarkers in the blood serum of 2 individuals with Austism Spectrum Disorder. We anticipated  that BDNF would be significantly up or down regulated and IFN-gamma up regulated as confirmed by the literature. see 10.1371/journal.pone.0020470 , DOI: 10.1371/journal.pone.0020470 and DOI: 10.1111/acps.12071.Our testing results showed:Figure 1: ASD and Biomarker LevelsBased on these initial findings we are developing a custom Quantibody® Antibody Array designed for the fine tuned testing of individuals with ASD. The biomarkers incl...
Source: Neuromics - December 30, 2013 Category: Neuroscience Tags: Antibody Array ASD autism spectrum disorder Autsim BDNF biomarkers Caspase 7 ELISA Heat Shock Proteins IFN-gamma INF-gamma TGF-beta Source Type: news

hMSCs and Mesen-X Media in Actions
Customer Feedback on Neuromics' Human Mesenchymal Stem CellsThe demand for these UCB derived hMSCs continues to grow. We do everything we can to insure users' success. This includes replacing cells if there are any issues.It is always great to get documented feedback. Dr. Rodney Nash, CEO of Javeen Biosciences used their new Mesen-X media to grow the cells. ThisGMP manufactured media is TOTALLY serum and animal free. It is shipped at room temperature and requires no attachment agents.Here's Dr. Nash's feedback: We recently used the hMSCs derived from Umbilical Cord Blood. Their performance was nothing less than excell...
Source: Neuromics - December 17, 2013 Category: Neuroscience Tags: Growth Media hMSCs Human Mesenchymal Stem Cells Javeen Biosciences Serum free media Source Type: news

Biochemistry of Hair Loss
In this study, Researchers use Tbx18Cre knockin mouse line to ablate the Wnt-responsive transcription factor β-catenin specifically in  at E14.5 during the first wave of guard hair follicle formation. In the absence of β-catenin, canonical Wnt signaling is effectively abolished in cell clusters of precursors for the hair follicle dermal papilla (DP). Sox2+ dermal condensates initiate normally; however by E16.5 guard hair follicle numbers are strongly reduced and by E18.5 most whiskers and guard hair follicles are absent, suggesting that active Wnt signaling in dermal condensates is important for hair follicl...
Source: Neuromics - December 7, 2013 Category: Neuroscience Tags: GFRA1 Antibody Wnt signaling; Dermal papilla cells; Stem cell niche; Hair follicle morphogenesis; Hair follicle stem cells Source Type: news

In vivo Like Cell Based Assays!
Save 100 USD on our Potent, Pure and Easy to Culture Stem and Progenitor CellsWe continue to design in vivo like behaviors into our  Cell Based Assays.Our goal is to enable you to make better research decisions earlierin your discovery process.These assays are engineered using our proven, potent and easy to culture eSC and Adult Human Stem Cells and Progenitors.UCB Derived hMSC differentiated to Osteoblast. Note the calcium depositions.***December Promotions-New Products-Save 100 USD+ on Primary and Stem Cells, Media, Markers and Growth Factors***Human Mesenchymal Stem Cells-Passage up to 12X-395 USD&nb...
Source: Neuromics - December 2, 2013 Category: Neuroscience Tags: CAFs hNP1 Neural Progenitors human Mesenchymal Stem Cell Based Assays human osteobalsts Osteogenesis Source Type: news

Microelectrodes for On-Chip Manipulation of Human Mesenchymal Stem Cells
Conclusion: Authors explored the effect of the dimensions of the rGO–PEDOT microelectrode arrays on the cell spreading and expression of differentiation; whereas the smaller rGO–PEDOT-20 array exhibited better cell alignment ability, the larger rGO–PEDOT-100 exhibited better performance at inducing osteocalcin expression in hMSCs. They also found that using three-zone parallel devices made it possible to release drugs at three points in time. This concept could presumably be extended to release different types of drugs at different lineages on defined patterns. Eventually, such devices might be appli...
Source: Neuromics - November 24, 2013 Category: Neuroscience Tags: 4-ethylenedioxythiophene) (PEDOT); drug release bioelectronics; graphene; poly(3 hMSCs human Mesenchymal Stem Cell Based Assays human osteobalsts Osteogenesis Assays Source Type: news

Neurotrack Player, Essen Bioscience & Neuromics Neurons
I claim that our solutions are thoroughly tested, validated and research ready. We measure our performance by making sure this claim rings true with our customers.One way this is confirmed to me is when a partner has success using one of our solutions in a new way. With that I am pleased to update you on Essen Bioscience's application for our primary neurons. Please note the related assays are being demonstrated in their most current webinar. We will be featuring kinetic and migration assays using our hMSCs, hOsteoblasts and hChondrocytes in the near future. So stay tuned. (Source: Neuromics)
Source: Neuromics - November 20, 2013 Category: Neuroscience Tags: e-18 Primary Rat Neurons Essen Bioscience Human Primary Neurons Kinentic NeuroTrack Assays Source Type: news

SMG-1 and Parkinson's Disease (PD)
Absence of SMG1 protein could lead to PDA new study has suggested that the absence of a protein called SMG1 - identified as a Regulator of Parkinson's disease-associated alpha-Synuclein-could aid in the development of Parkinson's and other related neurological disorders.In light of these findings, we believe SMG1 will have increasing importance for PD Researchers. We now have a solid marker for this protein.Image: Immunoperoxidase of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml inset: : Western blot of SMG1 expression in HeLa NE. Please check out our...
Source: Neuromics - November 1, 2013 Category: Neuroscience Tags: alpha-Synuclein Antibodies alpha-Synuclein Markers Parkinson ' s Disease SMG1 antibody Source Type: news

Our Antibody Arrays and ELISA Manufacturing Partner Receives ISO Certification
RayBiotech Awarded ISO 13485: 2003 Certification  Our partner, RayBiotech, provides us Antibody Arrays and ELISA Kits. This should give potential customers confirmation that this kit are rock solid. NORCROSS, GA -- (Marketwired) -- 09/11/13 -- RayBiotech, Inc. announced today that the company has been awarded ISO 13485: 2003 certification with respect to its compliance in the manufacture of in vitro diagnostics kits to be provided to the research community. The ISO 13485 award applies to multivariant antibody arrays, enzyme-linked immunosorbent assays (ELISAs) as well as membrane and glass format arrays and reage...
Source: Neuromics - October 26, 2013 Category: Neuroscience Tags: C-Series Antibody Arrays immune response inflammatory response Quantibody Arrays Raybiotech Sandwich ELISA Kits Source Type: news

Neuromics | suhani desai
Neuromics Website (Source: Neuromics)
Source: Neuromics - October 25, 2013 Category: Neuroscience Source Type: news

CIRM Stem Cell Research Updates: Autism syndrome modeled in lab dish points to futu...
: Here's a major stumbling block in developing therapies for human diseases -- ..Here's a major stumbling block in developing therapies for human diseases -- it's hard to find a fix if you don't really know what's wrong. Take autism spectrum disorders. By now doctors are pretty good at identifying signs of the disease, but without access to brain cells researchers don't really know what's going wrong. This work is being done by Dr. Ricardo Dolmetsch and his team at Stanford. They are particularly interested in understanding how electrical activity and calcium signals control the development of the brain and how this is alt...
Source: Neuromics - October 23, 2013 Category: Neuroscience Tags: autism spectrum disorder calcium channel Human Neurons Source Type: news

P2X3 Receptor and Inflammatory Nociception
Conclusions: Nociceptive response intensity was measured by observing the rat's behavior and considering the number of times the animal reflexively raised its hind paw (flinches) in 60 min. Local subcutaneous administration of the selective TRPA1, 5-HT3 or 5-HT1A receptor antagonists HC 030031, tropisetron and WAY 100,135, respectively, prevented the nociceptive responses induced by the administration in the same site of the non-selective P2X3 receptor agonist αβmeATP. Administration of the selective P2X3 and P2X2/3 receptor antagonist A-317491 or pretreatment with oligonucleotides antisense against P2X3 recepto...
Source: Neuromics - October 12, 2013 Category: Neuroscience Tags: Purinergic Receptor Antibodies inflammatory nociception Western Blot Nociceptors Pain Research P2X3 Receptor Antibody WB P2X3 Antibody Western Blotting Source Type: news

From Zero to 3-D Cell Based Assays in 15 Minutes
Collagel Hydrogels are Designed to Match Your Cell Types.We are pleased to add Collagel Hydrogels to our 3-D Cell Based Assay Solutions.The are 3 different gel types that mimic the different in vivo extracellular environment that your cells experience. You will be able to get really nice adipogenesis with MSCs using our CollaGel Hydrogel Standard. CollaGel Hydrogel Soft is an ideal matrix for growing fibroblast; primary hepatocyte cultures and great for growing smooth muscle cells. CollaGel Hydrogel Soft+ is an ideal matrix for growing nervous cells, veins cells and Hydroxyapatite crystals.Image: Primary hep...
Source: Neuromics - September 28, 2013 Category: Neuroscience Tags: 3-D Cell based Assays 3-D Cell Culturing Collagen ECMs Extracellular Matrix Hydrogel Type 1 Bovine Collagen Type 1 Human Collagen Type 1 Rat Collagen Source Type: news

New Astrocyte-Glial Markers
More Options!We get a lot of request for markers that will more specifically stain astrocytes, glia and microglia. Let's say, for example, you want to pinpoint these cell types in a mixed neuron-glial culture. You can now do a dual label and generate these results.Image: Neuron-glial cell mixture cultures stained with  ALDH1L1 (red) and our monoclonal antibody against GFAP (green). Blue is a DNA stain. ALDH1L1 stains astrocytes and excludes from neuron cells. ALDH1L1 stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes. Astrocytes are ...
Source: Neuromics - September 20, 2013 Category: Neuroscience Tags: Glial Fibrillary Acidic Protein Antibody Microglial Markers GFAP Antibody 10-formyltetrahydrofolate dehydrogenase antibody Astrocyte Markers FTHFDH antibody ALDH1L1 Antibody Source Type: news

Antibody Array-ELISA Kits for Cancer Researchers
We continue to build our catalog of Antibody Arrays & ELISA Kitsfor Cancer Researchers. Our RayBio® C-Series Ctyokine Arraysenable you to analyze the expression of up to 120 Cytokines for aboutthe price of a single ELISA (585 USD). Figure 3. PTEN Downregulation in HER2-Overexpressing Cells Activates an IL6/NF-kB-Mediated Inflammatory Feedback Loop (A–E) MCF7-HER2+PTEN cells secreted 3- to 5-fold higher levels of IL6, IL8, and CCL5 compared to MCF7-HER2+ or MCF7-PTEN cells as determined by RayBio human cytokine antibody Array 5 (A). The intensity of each blot compared to control was determined by Kodak im...
Source: Neuromics - September 15, 2013 Category: Neuroscience Tags: C-Series Antibody Arrays cancer markers Cancer Research Cytokines ELISA Kits. VEGFs Interleukins Raybio Raybiotech Source Type: news

Stem Cell Markers
Our Stem Cell Reagents our widely used and frequently published. We are proud of the positive feedback on our Stem Cell Markers.Image: Tuj-1 staining of Neuron-specific class III β-tubulin in differentiated human neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 (green) secondary antibody (Molecular Probe, A-11001) and counterstained with PI (red).Image: Neural progenitors were labeled with anti-rat Nestin polyclonal antibody (Cat#:GT15114) and stained with conjugated donkey anti-goat secondary anti-body (green). Differentiated neurons were labeled with neuron-specific mouse anti-&...
Source: Neuromics - September 9, 2013 Category: Neuroscience Tags: Stem Cell Research hN2 Neurons Cell Cultures Tuj 1 (Neuron-specific class III beta-tubulin) antibody hNP1 Neural Progenitors nestin antibodies Stem cell markers Source Type: news

Proud Distributor of RayBiotech's Antibody Arrays and ELISAs
Pioneer and Market LeaderI am pleased to announce we are a distribution partner for RayBiotech. The solutions we offer include Antibody Arrays and ELISA Kits. With these additions, you now have many options for the either the qualitative, semi-quantitative or quantitative measurement of protein expression. From single antibody pair ELISAs to multiplexed arrays. The arrays enable you to simultaneously analyze up to 80 cytokine, inflammatory response, growth factor or angiogenesis related proteins.In this posting, I focus on our new C-Series Antibody Array Kits. The kits utilize the sandwich immunoassay principle, wherein a ...
Source: Neuromics - August 21, 2013 Category: Neuroscience Tags: sandwich ELISA Cytokine signaling Cytokine Antibody Arrays Inflammatory Response Antibody Arrays Angiogenesis Antibody Arrays. Quantibody Arrays C-Series Antibody Arrays Source Type: news

A Method for 3D Immunostaining and Optical Imaging of the Mouse Brain
In this study our Chicken Nestin Antibody is used to stain these progenitors in dissected mouse brains: Gleave JA, Lerch JP, Henkelman RM, Nieman BJ (2013) A Method for 3D Immunostaining and Optical Imaging of the Mouse Brain Demonstrated in Neural Progenitor Cells. PLoS ONE 8(8): e72039. doi:10.1371/journal.pone.0072039. 3D antibody staining of adult mouse brains: 1% PFA perfused adult mouse brains were removed from the skull and then divided by removing the cerebellum and separating the hemispheres if desired. The samples were immediately dehydrated in a gradient of methanol solutions to 100% methanol over the ...
Source: Neuromics - August 12, 2013 Category: Neuroscience Tags: Immunostaining 3-D Assays. 3-D imaging mouse brain Immunofluorescence nestin antibody Neural Progenitor Cells Source Type: news

Healthy and Happy Neuron/Astrocytes Cultures
A Track Record of Customer SuccessNeuromics is  recognized for the quality of  hNP1™ Human Neural Progenitor,  hN2™ Neuron Discovery Kits, E18 and E20 Rat Primary Neurons and E18 Rat Primary Astroglia. As the company owner, it is important that I keep my finger on the pulse of how well they work for each and every unique application. I personally follow up with each user and if there are any issues, we replace the cells once free of charge. Your success is critical to our growth.I wanted to share with you recent references. These give and excellent snapshot of the exciting ways our cells can used...
Source: Neuromics - August 7, 2013 Category: Neuroscience Tags: Astrocytes Astrocytic differentiation Primary Human Neurons hNP1 Neural Progenitors e-18 Primary Rat Cortical Neurons E18 Primary Hippocampal Neurons culturing hNP1 Neural Progenitors Source Type: news

eSC Derived hNP1 Neural Progenitors Astrocytic Differentiation
Protocol for Driving hNP1TM Human Neural Progenitors to AstrocytesThere is a great demand for an easy way to generate human astrocytes in culture. I am pleased to present a protocol for differentiating our hNP1 Cells to Astrocytes. This comes from my friend Dr. Steve Stice and his team at ArunA Biomedical and University of Georgia: Majumder A, Dhara SK, Swetenburg R, Mithani M, Cao K, Medrzycki M, Fan Y, Stice SL. Inhibition of DNA methyltransferases and histone deacetylases induces astrocytic differentiation of neural progenitors. Stem Cell Res. 2013 Jul;11(1):574-86. doi: 10.1016/j.scr.2013.03.003. Epub 2013 Apr 2.These ...
Source: Neuromics - July 28, 2013 Category: Neuroscience Tags: Astrocytes Astrocytic differentiation BMP Primary Human Neurons hNP1 Neural Progenitors LIF cell culturing protocol culturing hNP1 Neural Progenitors GFAP Source Type: news

MAP-2-A Versatile Neuron Marker
Neuromics is a leader in providing Neuron-Glial Markers for Neuroscientists.We are constantly on the search for publications that reference use of these markers in unique applications. In this posting I would like to share a publication where researchers used on of our MAP-2 antibodies to stain medial superior olive (MSO) neurons. Baumann Veronika, Lehnert Simon, Leibold Christian, Koch Ursula. Tonotopic Organization of the Hyperpolarization-activated Current (Ih) in the Mammalian Medial Superior Olive. Front. Neural Circuits 7:117. doi: 10.3389/fncir.2013.00117. ...Following recording, slices were fixed in 4% pa...
Source: Neuromics - July 16, 2013 Category: Neuroscience Tags: MAP2 antibody Immunostaining Neuron Markers Microtubule-Associated Protein2 Antibody medial superior olive Glial Markers Immunfluorescence MSO Neurons Source Type: news

Using hESCs to Understand Vasculogenesis Processes
The Role of BMP4 Plays in Regulating Vascular DevelopmentMy friend, Dr. Steve Stice and team at UGA have published excellent findings on the process of vasculogenesis: N. L. Boyd, S. K. Dhara, R. Rekaya, E. A. Godbey, K. Hasneen, R. R. Rao, F. D. West III, B. A. Gerwe, S. L. Stice. BMP4 Promotes Formation of Primitive Vascular Networks in Human Embryonic Stem Cell–Derived Embryoid Bodies. Exp Biol Med (Maywood) June 2007 vol. 232 no. 6 833-843. Highlights:Human embryonic stem cells (hESC) have the capability to produce all of the cells of the body and have been used as in vitro models to study the...
Source: Neuromics - July 3, 2013 Category: Neuroscience Tags: human embryonic stem cell markers hESCs Regulating Vascular Development Vasculogenesis BMP-4 angiogenesis bone morphogenetic protein-4 Source Type: news

Protocol for Flow Cytometric Sorting of Enriched Neuronal Cultures from iPSCs
Surface molecule profiles undergo dynamic changes in physiology and pathology, serve as markers of cellular state and phenotype and can be exploited for cell selection strategies and diagnostics. The isolation of well-defined cell subsets is needed for in vivo and in vitro applications in stem cell biology. In this technical report, the authors present an approach for defining a subset of interest in a mixed cell population by flow cytometric detection of intracellular antigens. They have developed a fully validated protocol that enables the co-detection of cluster of differentiation (CD) surface antigens on...
Source: Neuromics - June 25, 2013 Category: Neuroscience Tags: Stem Cell Research neuronal cultures induced pluripotent stem (iPS) cells CD Markers. Flow Cytometry cell sorting Neuronal Markers Neuronal Differentation nestin antibody human Source Type: news

Super-Charging Pluripotent Stem Cells
Adapting Stem Cells to Cellular Stress for Regenerative Medicine and Cell-Based Therapies This approach for the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells could represent a breakthrough. The authors have identified these cells as "Multilineage Differentiating Stress-Enduring (MUSE) Cells": Heneidi S, Simerman AA, Keller E, Singh P, Li X, et al. (2013) Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE 8(6): e64752. doi:10.1371/journal.pone.0064752.Highl...
Source: Neuromics - June 18, 2013 Category: Neuroscience Tags: MUSE hypoxia preconditioning Multilineage Differentiating Stress-Enduring Cells MSCs HPC regenerative medicine cell based therapies Human Mesenchymal Stem Cells Human Adipose Tissue Progenitors Source Type: news

mGluR5 and Fragile-X Syndrome (FXS)
Conclusions:This study was the first to identify upregulation of mGluR5 density and expression in the prefrontal cortex of FXS patients or carriers compared to an age- and sex-matched control group. This is consistent with several studies in FXS model mice that postulate that the syndromic features of FXS are caused by an upregulated mGluR5 signaling pathway. Although the sample size was relatively small and the results could be secondary to prior medication treatment, these initial findings provide strong rationale for measuring mGluR5 in live patients using PET. Such in-vivo studies could measure mGluR5 in all brain regi...
Source: Neuromics - June 7, 2013 Category: Neuroscience Tags: ; mGluR Human Cortex Metabotropic glutamate receptor type 5 Western Blot Fragile X mental retardation Metabotropic glutamate receptor; mGluR5 Western Blotting FXS Source Type: news

Quantibody® Cytokine Arrays
Cytokines, Adipokines, Growth Factors and NeurotrophinsWe are pleased to announce addition of Antibody Arrays to our catalog. These are excellent tools for study apoptosis, inflammation, angiogenesis and  immune response and related autoimmune, inflammatory and degenerative diseases plus many forms of cancers. Check out this video.Specific Research Applications Include:•High-throughput profiling of cytokine expression•Validation of semi-quantitative antibody array results•Identifying potential molecular targets for drug development•Identifying the molecular mechanisms of drug action•Identifyin...
Source: Neuromics - May 27, 2013 Category: Neuroscience Tags: inflammatory response neuroinflammatory cytokines Cytokine signaling Adipokines Neurotrophins Antibody Arrays Growth factors Autommune disease Neurodegenerative Disease multiplex assays Source Type: news

Schwann Cell-Sensory Neurons-PNS Markers
Data-PublicationsThese markers a important tools for the study of Neuro-muscular diseases like Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS).For Neuromuscular Disease Researchers, we have some of the best Schwann Cell and Sensory/Peripheral Neuron Markers in the business.Images: Rat mixed neuron/glial cultures stained with Peripherin (green channel) and Neurofilament alpha-internexin/NF66 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since th...
Source: Neuromics - May 25, 2013 Category: Neuroscience Tags: sensory neurons Neurofilament Markers PGP9.5 Antibody neurolemmocytes Multiple Sclerosis Schwann Cell Markers Alpha 2a Adrenergic Receptor p75 antibody NGF marker Amyotrophic Lateral Sclerosis Source Type: news

The Role of Obesity in Obstructive Sleep Apnea (OSA)
This study shows the increase in the expression of leptins like Ob-Rb in Carotid .Images: Fluorescent (a-c) photomicrographs showing the effect of IH on Ob-Rb (a) and ERK 1/2 (b) expression in carotid body glomus cells. Note that Ob-Rb and ERK 1/2 are co-expressed in the same cells (c). Calibration mark in (a) represents 100 μm and applies to (a-c).Images: Western blots showing the presence of Ob-Rb (a) and Ob-R100 (b) in carotid bodies after IH. Note that IH significantly (*) decreases the protein level of Ob-Rb (a), while significantly (*) increasing the protein level of Ob-R100 (b) compared to normoxic controls (b). ...
Source: Neuromics - May 18, 2013 Category: Neuroscience Tags: immunoblotting Immunhistochemistry Obstructive sleep apnea LepRb antibody obesity Leptin Expression Ob-Rb antibody Western Blotting Immunfluorescence Source Type: news

Tissue Acidosis and Inflammation Related Nociceptive Pain
Important Implications for Inflammatory Pain StatesInflammatory pain is often accompanied by a drop in pH (Acidosis). Here investigators hypothesized that modest drops in extracellular pH leading to calcium fluxes acts like a dynamic switch to rapidly mobilize trkA to the cell membrane surface of adult sensory neurons, which in turn serves to increase the sensitivity of these neurons to NGF. The findings reveal a cellular mechanism whereby even small changes in pH can rapidly shift sensitivity to a critical driver of the inflammatory pain state—NGF. They way they demonstrate this shift is quite ingenious: Geoffrey E....
Source: Neuromics - May 11, 2013 Category: Neuroscience Tags: Inflammatory pain CGRP NGF ACIC3 phospho-TrkA antibody Substance P P2X3 TRPV1 Tissue Acidosis Source Type: news

More on the Neurobiology of Itch
I have an earlier posting on neuro-transmission of itch or pruritis. This focused on the role of Toll-like Receptor 3 (TLR 3). This posting looks at the role of Gastrin-Releasing Protein (GRP) and the Gastrin-Releasing Protein Receptor (CRPR) in chronic itch. The findings are important because this condition affects millions worldwide and results in a costly erosion of quality of life.Here chronic itch was studied in Macque Monkeys over a period of 4 years. The expression patterns of GRP, GRPR and PGP9.5 were accessed by immunohistochemistry: Leigh A Nattkemper, Zhong-Qiu Zhao, Anna J Nichols, Alexandru D P Papoiu, Carol A...
Source: Neuromics - May 7, 2013 Category: Neuroscience Tags: Protein Gene Product 9.5 Pruritus PGP9.5 Antibody Immunohistochemistry gastrin-releasing peptide; GRPR Chronic itch gastrin-releasing peptide receptor Source Type: news

Neuron-Glial Cultures-Setting a Higher Bar!
Improved Methods for Long Term, High Denisty Cultures Dr. Randen Patterson and his team at UC Davis have developed new culturing techniques using our e18 Rat Primary Hippocampal Neurons. They have developed a protocol that allows for culturing of E18 hippocampal neurons at high densities for more than 120 days. These cultured hippocampal neurons are (i) well differentiated with high numbers of synapses, (ii) anchored securely to their substrate, (iii) have high levels of functional connectivity, and (iv) form dense multi-layered cellular networks. We propose that our culture methodology is likely to be effective for m...
Source: Neuromics - April 29, 2013 Category: Neuroscience Tags: MAP2 antibody neuronal cultures Long term cell cultures. high denisty cultures neuron-glial based assays Neuronal Markers cell culturing protocol Hippocampal Neurons E18 Primary Hippocampal Neurons Source Type: news

Small Molecules-Peptides for Neuroscience Research
Agonists, Antagonists, Inhibitors and Ligands for Studying NeuromodulationOur friends at R and D Systems/Tocris Bioscience have made available to us select Small Molecules/Peptides. Our focus will be on providing agonists, antagonists, inhibitors and ligands that complement our Neuroscience and Pain Research products and expertise.2-APB=TRP Channel ModulatorWe will be adding about 10 new molecules/peptides per month. Here's a sampling our our most recent additions:NameTypeBioactivity(±)-trans-ACPDAgonistPotent NMDA agonist. Also group II mGluR agonist(S)-(-)-5-FluorowillardiineAgonistVery potent AMPA agonist(S)-4-Ca...
Source: Neuromics - April 22, 2013 Category: Neuroscience Tags: agonist Mu Opioid Receptor Neuromodulation P2X3 Synaptic Plasticity ligands antagonists Opioid Receptors neuroscience inhibitors TRPV1 logands Nociception Neurotransmission Source Type: news

P2X3 Receptors and Migraine
P2X3 Receptors of Trigeminal Sensory Neurons and Familial Hemiplegic Migraine Type 1 (FHM-1). Our P2X Receptor Markers continue to be used in interesting and novel ways. Here researchers use our P2X3 Receptor Antibody to study expression using primary rat ganlia cultures: Swathi K. Hullugundi,Michel D. Ferrari, Arn M. J. M. van den Maagdenberg, Andrea Nistri. Andrea Nistri. The Mechanism of Functional Up-Regulation of P2X3 Receptors of Trigeminal Sensory Neurons in a Genetic Mouse Model of Familial Hemiplegic Migraine Type 1 (FHM-1). PLoS ONE 8(4): e60677. doi:10.1371/journal.pone.0060677 Abstract: A knock-in (KI) mouse mo...
Source: Neuromics - April 14, 2013 Category: Neuroscience Tags: sensory neurons Immunhistochemistry Purinergic Receptor Antibodies Familial Hemiplegic Migraine Type 1 Dorsal Root Ganglia Nociceptive Pain Nociception P2X3 Antibody Source Type: news

Science Behind Elecroacupuncture for Treating Shingles
Conclusions: EA treatment improves thermal perception by recovering TRPV1-positive sensory neuronsand nerve terminals damaged by RTX. EA Also reduces RTX-induced tac tile allodynia by attenuating the damage of myelinated afferent nerves and their abnormal sprouting into the spinal lamina II. Our study provides new information about the mechanisms of the therapeutic actions of EA in the treatment of PHN. Figure 2:  Effect of EA on RTX-induced deletion of TRPV1-immunoreactive neuron s in the DRG. A, Representative images showing TRPV1-immunoreactive neurons in the lumbar DRG of vehicle ( a ) , RTX ( b ), RTX plus 2 Hz E...
Source: Neuromics - April 4, 2013 Category: Neuroscience Tags: TRPV1 antibodies Immunhistochemistry postherpetic neuralgia Dorsal Root Ganglia Dorsal horn shingles TRPV1-N Antibody Immunfluorescence VR1-N Antibody Source Type: news

hN2 Human Neurons for Toxicity Screening
Our Human hN2 Neurons proving excellent platforms for Neurotoxicology Studies. I would like to share recent publication and that confirms the potential of these solutions for use in your toxicology assays. Xiugong Gao, Hsiuling Lin, Radharaman Ray, Prabhati Ray. Toxicogenomic Studies of Human Neural Cells Following Exposure to Organophosphorus Chemical Warfare Nerve Agent VX. Neurochemical Research. February 2013.Abstract: Organophosphorus (OP) compounds represent an important group of chemical warfare nerve agents that remains a significant and constant military and civilian threat. OP compounds are considered acting...
Source: Neuromics - March 26, 2013 Category: Neuroscience Tags: hN2 Neurons Cell Cultures Neurotoxicity Highthroughput Toxicity Screening Nerve agent VX hN2 Kits Human Primary Neurons Human Neurons Source Type: news