Bioactive FGF Basic Recombinant Protein in Action

Maintaining Sheep Mesenchymal Stem Cell MultipotencyWe have been very selective in the Bioactive FGF2 or FGF-basic Recombinant Proteins we make available to Stem Cell Researchers. They are many options available so we are always encouraged when we see ours referenced in publications.Here researchers used our FGF2 to expand Sheep Bone Marrow Stem Cells (BMSCs) in culture: Troy D Bornes, Nadr M Jomha, Aillette Mulet-Sierra and Adetola B Adesida. Hypoxic culture of bone marrow-derived mesenchymal stromal stem cells differentially enhances in vitro chondrogenesis within cell-seeded collagen and hyaluronic acid porous scaffolds.Stem Cell Research & Therapy 2015, 6:84 doi:10.1186/s13287-015-0075-4.These cells were used for chondrogenesis studies.Expansion of BMSCs: Bone marrow aspirate collections containing 8 x 107 MNCs were seeded within each 150-cm2 tissue culture flask. Culture medium composed of alpha-minimal essential medium (α-MEM) supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS), penicillinstreptomycin-glutamine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and sodium pyruvate (all from Life Technologies, Burlington, Canada) was pipetted into each flask. Fibroblast growth factor-2 (FGF-2; Neuromics Inc., Edina, USA) was added at a concentration of 5 ng/ml in order to maintain cell multipotency. Nucleated cells were allowed to adhere and grow for seven days before the first media change under normoxia (ambient 21% O2) or hypoxia (l...
Source: Neuromics - Category: Neuroscience Tags: Chrondrocytes FGF Basic FGF Basic Recombinant Protein Fibroblast Growth Factor Basic Human Mesenchymal Stem Cells MSCs Source Type: news