3-D Screens Predict Breast Cancer Metastasis

Cell-ECM Interactions3-D culturing solutions and methods are increasingly being used to created more in vivo like assays.Here researchers use our Human Collagen IV extracellular matrix protein (ECM) to develop a simple biomaterial platform with systematic control over the ECM protein density and composition to determine if integrin binding governs how metastatic cells differentiate between secondary tissue sites: L.E. Barney, E.C. Dandley, L.E. Jansen, N.G. Reich, A.M. Mercurio, and S.R. Peyton. A cell–ECM screening method to predict breast cancer metastasis. Integr Biol (Camb). 2015 Feb 10; 7(2): 198–212. doi: 10.1039/c4ib00218k.This publication details the creation of an in vitro fingerprint that is predictive of in vivo metastasis.Figure: Biomaterial platform for integrin-mediated phenotyping. (a) Breast cancer cell lines with their known in vivo metastatic tropisms.  (b) Three distinct ECM microenvironments regulate integrin binding. (c) Adhesion and motility phenotypes of the MDA-MB-231 cell line. Black: ECM 1; blue: ECM 2; green: ECM 3.Figure: Correlations between adhesion and migration responses identify potent integrin antibodies in vitro. Pairwise comparisons between adhesion and migration measurements in the (a, b) MDA-MB-231 and (c, d) SkBr3 cell lines across normal, EGF-stimulated, and integrin antibody conditions. Arrows highlight conditions where integrin antibodies increased migration metrics. Spearman correlations are indicated on each plot with ...
Source: Neuromics - Category: Neuroscience Tags: 3D collagen scaffolds breast cancer ECMs Extracellular Matrix metastasis Source Type: news