Supplementation of nicotinamide mononucleotide diminishes COX-2 associated inflammatory responses in macrophages by activating kynurenine/AhR signaling
Free Radic Biol Med. 2024 Feb 8;214:69-79. doi: 10.1016/j.freeradbiomed.2024.01.046. Online ahead of print.ABSTRACTCyclooxygenase-2 (COX-2) is an inducible enzyme responsible for prostaglandin synthesis during inflammation and immune responses. Our previous results show that NAD+ level decreased in activated macrophages while nicotinamide mononucleotide (NMN) supplementation suppressed the inflammatory responses via restoring NAD+ level and downregulating COX-2. However, whether NMN downregulates COX-2 in mouse model of inflammation, and its underlying mechanism needs to be further explored. In the present study, we establ...
Source: Free Radical Biology and Medicine - February 9, 2024 Category: Biology Authors: Jing Liu Wenxuan Hou Zhaoyun Zong Yuling Chen Xiaohui Liu Ran Zhang Haiteng Deng Source Type: research
Sterile inflammation induces vasculopathy and chronic lung injury in murine sickle cell disease
Free Radic Biol Med. 2024 Feb 7:S0891-5849(24)00060-1. doi: 10.1016/j.freeradbiomed.2024.01.052. Online ahead of print.ABSTRACTMurine sickle cell disease (SCD) results in damage to multiple organs, likely mediated first by vasculopathy. While the mechanisms inducing vascular damage remain to be determined, nitric oxide bioavailability and sterile inflammation are both considered to play major roles in vasculopathy. Here, we investigate the effects of high mobility group box-1 (HMGB1), a pro-inflammatory damage-associated molecular pattern (DAMP) molecule on endothelial-dependent vasodilation and lung morphometrics, a struc...
Source: Free Radical Biology and Medicine - February 9, 2024 Category: Biology Authors: Kevin R Rarick Keguo Li Ru-Jeng Teng Xigang Jing Dustin P Martin Hao Xu Deron W Jones Neil Hogg Cheryl A Hillery Guilherme Garcia Billy W Day Stephen Naylor Kirkwood A Pritchard Source Type: research
Sestrin2 reduces ferroptosis via the Keap1/Nrf2 signaling pathway after intestinal ischemia-reperfusion
Free Radic Biol Med. 2024 Feb 6:S0891-5849(24)00065-0. doi: 10.1016/j.freeradbiomed.2024.02.003. Online ahead of print.ABSTRACTSestrins are metabolic regulators that respond to stress by reducing the levels of reactive oxygen species (ROS) and inhibiting the activity of target of rapamycin complex 1 (mTORC1). Previous research has demonstrated that Sestrin2 mitigates ischemia-reperfusion (IR) injury in the heart, liver, and kidneys. However, its specific role in IIR injury remains unclear. To elucidate the role of Sestrin2 in intestinal ischemia-reperfusion injury, we conducted an experimental study using a C57BL/6J mouse ...
Source: Free Radical Biology and Medicine - February 8, 2024 Category: Biology Authors: Le-le Zhang Ke Ding Shi-Shi Liao Yi-Guo Zhang Hui-Yang Liao Rong Chen Qing-Tao Meng Source Type: research
Corrigendum to "NADPH oxidases mediate a cellular "memory" of angiotensin II stress in hypertensive cardiac hypertrophy" [Free Radic. Biol. Med. 65 (2013) 897-907]
Free Radic Biol Med. 2024 Feb 6;214:1. doi: 10.1016/j.freeradbiomed.2024.01.051. Online ahead of print.NO ABSTRACTPMID:38325065 | DOI:10.1016/j.freeradbiomed.2024.01.051 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - February 7, 2024 Category: Biology Authors: Hong-Xia Wang Hui Yang Qiu-Yue Han Nan Li Xue Jiang Cui Tian Jie Du Hui-Hua Li Source Type: research
Elaborate cooperation of poly(rC)-binding proteins 1/2 and glutathione in ferroptosis induced by plasma-activated Ringer's lactate
In conclusion, PAL can selectively sensitize MM cells to ferroptosis by remodeling cytoplasmic iron homeostasis, where glutathione and PCBPs play distinct roles, resulting in lethal ferritinophagy and mitochondrial dysfunction. Our findings indicate the clinical application of PAL as a ferroptosis-inducer and the potential of PCBPs as novel targets in cancer therapeutics.PMID:38325565 | DOI:10.1016/j.freeradbiomed.2024.02.001 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - February 7, 2024 Category: Biology Authors: Li Jiang Hao Zheng Moe Ishida Qinying Lyu Shinya Akatsuka Yashiro Motooka Kotaro Sato Yoshitaka Sekido Kae Nakamura Hiromasa Tanaka Kenji Ishikawa Hiroaki Kajiyama Masaaki Mizuno Masaru Hori Shinya Toyokuni Source Type: research
Bisphenol A induces placental ferroptosis and fetal growth restriction via the YAP/TAZ-ferritinophagy axis
Free Radic Biol Med. 2024 Mar;213:524-540. doi: 10.1016/j.freeradbiomed.2024.01.033. Epub 2024 Feb 6.ABSTRACTExposure to bisphenol A (BPA) during gestation leads to fetal growth restriction (FGR), whereby the underlying mechanisms remain unknown. Here, we found that FGR patients showed higher levels of BPA in the urine, serum, and placenta; meanwhile, trophoblast ferroptosis was observed in FGR placentas, as indicated by accumulated intracellular iron, impaired antioxidant molecules, and increased lipid peroxidation products. To investigate the role of ferroptosis in placental and fetal growth, BPA stimulation was performe...
Source: Free Radical Biology and Medicine - February 7, 2024 Category: Biology Authors: Yanan Sun Menghan Sha Yu Qin Juan Xiao Wei Li Shufang Li Suhua Chen Source Type: research
BRG1 accelerates mesothelial cell senescence and peritoneal fibrosis by inhibiting mitophagy through repression of OXR1
In conclusion, BRG1 accelerates mesothelial cell senescence and peritoneal fibrosis by inhibiting mitophagy through repression of OXR1. This indicates that modulating BRG1-OXR1-mitophagy signaling may represent an effective treatment for PD-related peritoneal fibrosis.PMID:38311259 | DOI:10.1016/j.freeradbiomed.2024.01.054 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - February 4, 2024 Category: Biology Authors: Shuting Li Yiyi Zhuang Yue Ji Xiaowen Chen Liying He Sijia Chen Yating Luo Lingyu Shen Jing Xiao Huizhen Wang Congwei Luo Fenfen Peng Haibo Long Source Type: research
Targeting ZIP8 mediated ferroptosis as a novel strategy to protect against the retinal pigment epithelial degeneration
In this study, we identified that ZIP8, a metal-ion transporter, plays a crucial role in the degeneration of RPE cells mediated by ferroptosis. ZIP8 was found to be upregulated in patients with AMD through transcriptome analysis. Upregulated ZIP8 was also observed in both oxidative-stressed RPE cells and AMD mouse model. Importantly, knockdown of ZIP8 significantly inhibited ferroptosis in RPE cells induced by sodium iodate-induced oxidative stress. Blocking ZIP8 with specific antibodies reversed RPE degeneration and restored retinal function, improving visual loss in a mouse model of NaIO3-induced. Interestingly, the modi...
Source: Free Radical Biology and Medicine - February 3, 2024 Category: Biology Authors: Ziling Liu Jianguo Huang Deshuang Li Chuanhe Zhang Huan Wan Bing Zeng Yao Tan Fuhua Zhong Hongxia Liao MuYun Liu Zhe-Sheng Chen Chang Zou Dongcheng Liu Bo Qin Source Type: research
The opposite role of lactate dehydrogenase a (LDHA) in cervical cancer under energy stress conditions
Free Radic Biol Med. 2024 Feb 1;214:2-18. doi: 10.1016/j.freeradbiomed.2024.01.043. Online ahead of print.ABSTRACTDue to insufficient and defective vascularization, the tumor microenvironment is often nutrient-depleted. LDHA has been demonstrated to play a tumor-promoting role by facilitating the glycolytic process. However, whether and how LDHA regulates cell survival in the nutrient-deficient tumor microenvironment is still unclear. Here, we sought to investigate the role and mechanism of LDHA in regulating cell survival and proliferation under energy stress conditions. Our results showed that the aerobic glycolysis leve...
Source: Free Radical Biology and Medicine - February 2, 2024 Category: Biology Authors: Chaoran Jia Yulun Wu Feng Gao Wei Liu Na Li Yao Chen Luguo Sun Shuyue Wang Chunlei Yu Yongli Bao Zhenbo Song Source Type: research
Mitochondria: A new member involved in stem cell senescence. An extended commentary on "Mitochondria pleiotropism in stem cell senescence: Mechanisms and therapeutic approaches by Mas-Bargues C. [Free Radic. Biol. Med. (2023) Nov 1;208:657-671. doi:10.1016/j.freeradbiomed.2023.09.019]
Free Radic Biol Med. 2024 Mar;213:426-429. doi: 10.1016/j.freeradbiomed.2024.01.048. Epub 2024 Jan 30.NO ABSTRACTPMID:38301973 | DOI:10.1016/j.freeradbiomed.2024.01.048 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Matilde Alique Cristina Mas-Bargues Source Type: research
ULP-2 SUMO protease regulates UPR < sup > mt < /sup > and mitochondrial homeostasis in Caenorhabditis elegans
In this study, we show that the SUMO protease ULP-2 has a key role in regulating mitochondrial function and UPRmt. Specifically, down-regulation of ulp-2 suppresses UPRmt and reduces mitochondrial membrane potential without significantly affecting cellular ROS. Mitochondrial networks are expanded in ulp-2 null mutants with larger mitochondrial area and increased branching. Moreover, the amount of mitochondrial DNA is increased in ulp-2 mutants. Downregulation of ULP-2 also leads to alterations in expression levels of mitochondrial genes involved in protein import and mtDNA replication, however, mitophagy remains unaltered....
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Lirin Michaeli Eyal Spector Simon Haeussler C átia A Carvalho Hanna Grobe Ulrike Bening Abu-Shach Hen Zinger Barbara Conradt Limor Broday Source Type: research
Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma
This study aimed to determine whether zinc is associated with the ESCC and underlying molecular events involving malignant progression. Based on a case-control study, we found serum and urine zinc were decreased and correlated with ESCC progression. Thus, an in vitro model for zinc deficiency (ZD) was established, and we found that ZD contributed to the proliferation, migration, and invasion of EC109 cells. Untargeted metabolomics identified 59 upregulated metabolites and 6 downregulated metabolites, among which glycolysis and ferroptosis-related oxidation of chain fatty acids might play crucial steps in ZD-treated molecul...
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Peiyan Yang Hui Li Mingjun Sun Xinxin Guo Yinghao Liao Mohan Hu Ping Ye Ran Liu Source Type: research
Sulforaphane impedes mitochondrial reprogramming and histone acetylation in polarizing M1 (LPS) macrophages
This study investigated whether the phytochemical sulforaphane (Sfn) influences mitochondrial reprogramming during M1 polarization, as well as to what extent this can contribute to Sfn-mediated inhibition of M1 marker expression in murine macrophages. The use of extracellular flux-, metabolite-, and immunoblot analyses as well as fluorescent dyes indicative for mitochondrial morphology, membrane potential or superoxide production, demonstrated that M1 (LPS/Sfn) macrophages maintain an unbroken TCA cycle, higher OXPHOS rate, boosted fusion dynamics, lower membrane potential, and less superoxide production in their mitochond...
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Sheyda Bahiraii Martin Brenner Wolfram Weckwerth Elke H Heiss Source Type: research
Met-Exo attenuates mitochondrial dysfunction after hepatic ischemia-reperfusion injury in rats by modulating AMPK/SIRT1 signaling pathway
In this study, firstly, we detected that mitochondrial morphology and function were severely affected in hepatic tissues after hepatic IRI combined with partial resection, and then verified by in vitro experiments that Met-Exo could promote mitochondrial biosynthesis and fusion-associated protein expression and inhibit mitochondrial fission-related protein expression by modulating the AMPK/SIRT1 signalling pathway. This indicates that ADSCs-Exo can not only play a targeting role as a drug carrier but also has a great potential to act as a vehicle to act synergistically with drugs in the treatment of tissue and organ damage...
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Chenxi Piao Yue Wang Xiangyu Lu Tao Liu Yajun Ma Yuepeng Li Jiantao Zhang Hongbin Wang Source Type: research
Enhanced glutathione levels confer resistance to apoptotic and ferroptotic programmed cell death in NEIL DNA glycosylase deficient HAP1 cells
This study reveals an unforeseen role of NEIL glycosylases in regulation of resistance to oxidative stress, suggesting that modulation of NEIL glycosylase activities is a potential approach to improve the efficacy of e.g. anti-inflammatory therapies.PMID:38301978 | DOI:10.1016/j.freeradbiomed.2024.01.037 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - February 1, 2024 Category: Biology Authors: Christine Gran Neurauter Marco Pannone Mirta Mittelstedt Leal de Sousa Wei Wang Anna Ku śnierczyk Luisa Luna P ål Sætrom Katja Scheffler Magnar Bj ørås Source Type: research
