Lactoferrin ameliorated obesity-induced endothelial dysfunction by inhibiting the Tak1/IL-18/eNOS pathway between PVAT and vascular endothelium
In this study, we examined the influence of LF on ED and PVAT using high-fat diet mice as well as MAEC cells and 3T3-L1 adipocytes. Finally, LF supplementation decreases the systolic blood pressure (SBP), serum adhesion molecule (ICAM-1 and VCAM-1), and aorta ROS levels, and improves endothelium-dependent relaxation function in high-fat diet mice. Moreover, LF supplementation down-regulates the Tak1/IL-18/eNOS pathway between PVAT and aorta and enhances the NO generation in high-fat diet mice. In addition, we observe that LF decreases the expression levels of IL-18 and p-Tak1 in 3T3-L1 adipocytes, but fails to influence th...
Source: Free Radical Biology and Medicine - December 30, 2023 Category: Biology Authors: Cailong Chen Yilin Yan Yunxuan Wu Menglan Lu Yifei Xing Yujie Bai Haodong Zhao Li Ding Ying Wu Jiaying Xu Liqiang Qin Haitao Lv Zheng Zhang Source Type: research
SYNJ2BP ameliorates intervertebral disc degeneration by facilitating mitochondria-associated endoplasmic reticulum membrane formation and mitochondrial Zn < sup > 2+ < /sup > homeostasis
Free Radic Biol Med. 2023 Dec 27;212:220-233. doi: 10.1016/j.freeradbiomed.2023.12.028. Online ahead of print.ABSTRACTNucleus pulposus (NP) cell function-loss is one main contributor during intervertebral disc degeneration (IDD) progression. Both mitochondria and endoplasmic reticulum (ER) play vital roles in sustaining NP cell homeostasis, while the precise function of ER-mitochondria tethering and cross talk in IDD remain to be clarified. Here, we demonstrated that a notable disruption of mitochondria-associated ER membrane (MAM) was identified in degenerated discs and TBHP-induced NP cells, accompanied by mitochondrial ...
Source: Free Radical Biology and Medicine - December 29, 2023 Category: Biology Authors: Yu Song Wen Geng Dingchao Zhu Huaizhen Liang Zhi Du Bide Tong Kun Wang Shuai Li Yong Gao Xiaobo Feng Zhiwei Liao Rongcheng Mei Cao Yang Source Type: research
Hydrogen peroxide is not generated intracellularly in human neural spheroids during ischemia-reperfusion
Free Radic Biol Med. 2023 Dec 27;212:234-240. doi: 10.1016/j.freeradbiomed.2023.12.039. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are considered a primary source of damage during ischemic stroke. However, the precise timing of ROS production (during hypoxia or reperfusion) remains unclear. Cellular 3D spheroids are often proposed as an optimal alternative to both 2D cell cultures and animal models in modeling disease conditions. Here we report live imaging of hydrogen peroxide dynamics during the acute phase of hypoxia and reperfusion in human iPSC-derived neural spheroids, stably expressing fluorescent b...
Source: Free Radical Biology and Medicine - December 29, 2023 Category: Biology Authors: Veronika S Usatova Natalie M Mishina Mikhail A Berestovoy Alexander V Ivanenko David Jappy Viktoriya G Krut' Rostislav A Sokolov Aleksandr A Moshchenko Andrei Rozov Evgeny K Shevchenko Vsevolod V Belousov Source Type: research
Overexpression of manganese superoxide dismutase mitigates ACL injury-induced muscle atrophy, weakness and oxidative damage
Free Radic Biol Med. 2023 Dec 26;212:191-198. doi: 10.1016/j.freeradbiomed.2023.12.037. Online ahead of print.ABSTRACTOxidative stress has been implicated in the etiology of skeletal muscle weakness following joint injury. We investigated longitudinal patient muscle samples following knee injury (anterior cruciate ligament tear). Following injury, transcriptomic analysis revealed downregulation of mitochondrial metabolism-related gene networks, which were supported by reduced mitochondrial respiratory flux rates. Additionally, enrichment of reactive oxygen species (ROS)-related pathways were upregulated in muscle following...
Source: Free Radical Biology and Medicine - December 28, 2023 Category: Biology Authors: Christine M Latham Peyton J Balawender Nicholas T Thomas Alexander R Keeble Camille R Brightwell Ahmed Ismaeel Yuan Wen Jean L Fry Patrick G Sullivan Darren L Johnson Brian Noehren Allison M Owen Christopher S Fry Source Type: research
Overexpression of manganese superoxide dismutase mitigates ACL injury-induced muscle atrophy, weakness and oxidative damage
Free Radic Biol Med. 2023 Dec 26:S0891-5849(23)01193-0. doi: 10.1016/j.freeradbiomed.2023.12.037. Online ahead of print.ABSTRACTOxidative stress has been implicated in the etiology of skeletal muscle weakness following joint injury. We investigated longitudinal patient muscle samples following knee injury (anterior cruciate ligament tear). Following injury, transcriptomic analysis revealed downregulation of mitochondrial metabolism-related gene networks, which were supported by reduced mitochondrial respiratory flux rates. Additionally, enrichment of reactive oxygen species (ROS)-related pathways were upregulated in muscle...
Source: Free Radical Biology and Medicine - December 28, 2023 Category: Biology Authors: Christine M Latham Peyton J Balawender Nicholas T Thomas Alexander R Keeble Camille R Brightwell Ahmed Ismaeel Yuan Wen Jean L Fry Patrick G Sullivan Darren L Johnson Brian Noehren Allison M Owen Christopher S Fry Source Type: research
Overexpression of manganese superoxide dismutase mitigates ACL injury-induced muscle atrophy, weakness and oxidative damage
Free Radic Biol Med. 2023 Dec 26:S0891-5849(23)01193-0. doi: 10.1016/j.freeradbiomed.2023.12.037. Online ahead of print.ABSTRACTOxidative stress has been implicated in the etiology of skeletal muscle weakness following joint injury. We investigated longitudinal patient muscle samples following knee injury (anterior cruciate ligament tear). Following injury, transcriptomic analysis revealed downregulation of mitochondrial metabolism-related gene networks, which were supported by reduced mitochondrial respiratory flux rates. Additionally, enrichment of reactive oxygen species (ROS)-related pathways were upregulated in muscle...
Source: Free Radical Biology and Medicine - December 28, 2023 Category: Biology Authors: Christine M Latham Peyton J Balawender Nicholas T Thomas Alexander R Keeble Camille R Brightwell Ahmed Ismaeel Yuan Wen Jean L Fry Patrick G Sullivan Darren L Johnson Brian Noehren Allison M Owen Christopher S Fry Source Type: research
Overexpression of manganese superoxide dismutase mitigates ACL injury-induced muscle atrophy, weakness and oxidative damage
Free Radic Biol Med. 2023 Dec 26:S0891-5849(23)01193-0. doi: 10.1016/j.freeradbiomed.2023.12.037. Online ahead of print.ABSTRACTOxidative stress has been implicated in the etiology of skeletal muscle weakness following joint injury. We investigated longitudinal patient muscle samples following knee injury (anterior cruciate ligament tear). Following injury, transcriptomic analysis revealed downregulation of mitochondrial metabolism-related gene networks, which were supported by reduced mitochondrial respiratory flux rates. Additionally, enrichment of reactive oxygen species (ROS)-related pathways were upregulated in muscle...
Source: Free Radical Biology and Medicine - December 28, 2023 Category: Biology Authors: Christine M Latham Peyton J Balawender Nicholas T Thomas Alexander R Keeble Camille R Brightwell Ahmed Ismaeel Yuan Wen Jean L Fry Patrick G Sullivan Darren L Johnson Brian Noehren Allison M Owen Christopher S Fry Source Type: research
Remote ischemic preconditioning reduces mitochondrial apoptosis mediated by calpain 1 activation in myocardial ischemia-reperfusion injury through calcium channel subunit Cacna2d3
Free Radic Biol Med. 2023 Dec 25;212:80-93. doi: 10.1016/j.freeradbiomed.2023.12.030. Online ahead of print.ABSTRACTRemote Ischemic Preconditioning (RIPC) can reduce myocardial ischemia-reperfusion injury, but its mechanism is not clear. In order to explore the mechanism of RIPC in myocardial protection, we collected myocardial specimens during cardiac surgery in children with tetralogy of Fallot for sequencing. Our study found RIPC reduces the expression of the calcium channel subunit cacna2d3, thereby impacting the function of calcium channels. As a result, calcium overload during ischemia-reperfusion is reduced, and the...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Guoyang Liu Yong Lv Yanting Wang Zhenzhen Xu Lu Chen Shiqiang Chen Wanli Xie Yiqi Feng Jie Liu Yunxiao Bai Yuyao He Xia Li Qingping Wu Source Type: research
Potassium ion efflux induces exaggerated mitochondrial damage and non-pyroptotic necrosis when energy metabolism is blocked
Free Radic Biol Med. 2023 Dec 25:S0891-5849(23)01186-3. doi: 10.1016/j.freeradbiomed.2023.12.029. Online ahead of print.ABSTRACTDamage-associated molecular patterns (DAMPs) such as extracellular ATP and nigericin (a bacterial toxin) not only act as potassium ion (K+) efflux inducers to activate NLRP3 inflammasome, leading to pyroptosis, but also induce cell death independently of NLRP3 expression. However, the roles of energy metabolism in determining NLRP3-dependent pyroptosis and -independent necrosis upon K+ efflux are incompletely understood. Here we established cellular models by pharmacological blockade of energy met...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Rong Xu Li-Sha Yuan Ying-Qing Gan Na Lu Ya-Ping Li Zhi-Ya Zhou Qing-Bing Zha Xian-Hui He Tak-Sui Wong Dong-Yun Ouyang Source Type: research
Serum peroxiredoxin-4, a biomarker of oxidative stress, is associated with the development of nephropathy in patients with type 2 diabetes (Zodiac-65)
In conclusion, circulating peroxiredoxin-4 associates positively associates with an increased risk of developing nephropathy in T2D independent and irrespective of low-grade inflammation.PMID:38151214 | DOI:10.1016/j.freeradbiomed.2023.12.025 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Arno R Bourgonje Harry van Goor Stephan J L Bakker Jan-Luuk Hillebrands Henk J G Bilo Robin P F Dullaart Peter R van Dijk Source Type: research
Oxidative stress related effect of xenobiotics on eukaryotic model organism, Saccharomyces cerevisiae
Free Radic Biol Med. 2023 Dec 25:S0891-5849(23)01189-9. doi: 10.1016/j.freeradbiomed.2023.12.033. Online ahead of print.ABSTRACTEcotoxicological assays have traditionally focused on the effects of chemicals at the individual level by exploiting mortality and reproduction as endpoints. Although these two parameters are ecologically relevant, they rarely provide information regarding the elemental toxic mechanisms. Obviously, the number of xenobiotics used has been rapidly increased. Thus, any established measurement that shortens the actual outcome and, simultaneously provides information about toxic mechanisms is desirable...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Panwad Sillapawattana Martin Clemen Horst Gruhlke Thomas-Benjamin Seiler Prapaipat Klungsupya Wasin Charerntantanakul Source Type: research
Remote ischemic preconditioning reduces mitochondrial apoptosis mediated by calpain 1 activation in myocardial ischemia-reperfusion injury through calcium channel subunit Cacna2d3
Free Radic Biol Med. 2023 Dec 25:S0891-5849(23)01185-1. doi: 10.1016/j.freeradbiomed.2023.12.030. Online ahead of print.ABSTRACTRemote Ischemic Preconditioning (RIPC) can reduce myocardial ischemia-reperfusion injury, but its mechanism is not clear. In order to explore the mechanism of RIPC in myocardial protection, we collected myocardial specimens during cardiac surgery in children with tetralogy of Fallot for sequencing. Our study found RIPC reduces the expression of the calcium channel subunit cacna2d3, thereby impacting the function of calcium channels. As a result, calcium overload during ischemia-reperfusion is redu...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Guoyang Liu Yong Lv Yanting Wang Zhenzhen Xu Lu Chen Shiqiang Chen Wanli Xie Yiqi Feng Jie Liu Yunxiao Bai Yuyao He Xia Li Qingping Wu Source Type: research
Potassium ion efflux induces exaggerated mitochondrial damage and non-pyroptotic necrosis when energy metabolism is blocked
Free Radic Biol Med. 2023 Dec 25:S0891-5849(23)01186-3. doi: 10.1016/j.freeradbiomed.2023.12.029. Online ahead of print.ABSTRACTDamage-associated molecular patterns (DAMPs) such as extracellular ATP and nigericin (a bacterial toxin) not only act as potassium ion (K+) efflux inducers to activate NLRP3 inflammasome, leading to pyroptosis, but also induce cell death independently of NLRP3 expression. However, the roles of energy metabolism in determining NLRP3-dependent pyroptosis and -independent necrosis upon K+ efflux are incompletely understood. Here we established cellular models by pharmacological blockade of energy met...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Rong Xu Li-Sha Yuan Ying-Qing Gan Na Lu Ya-Ping Li Zhi-Ya Zhou Qing-Bing Zha Xian-Hui He Tak-Sui Wong Dong-Yun Ouyang Source Type: research
Serum peroxiredoxin-4, a biomarker of oxidative stress, is associated with the development of nephropathy in patients with type 2 diabetes (Zodiac-65)
In conclusion, circulating peroxiredoxin-4 associates positively associates with an increased risk of developing nephropathy in T2D independent and irrespective of low-grade inflammation.PMID:38151214 | DOI:10.1016/j.freeradbiomed.2023.12.025 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Arno R Bourgonje Harry van Goor Stephan J L Bakker Jan-Luuk Hillebrands Henk J G Bilo Robin P F Dullaart Peter R van Dijk Source Type: research
Oxidative stress related effect of xenobiotics on eukaryotic model organism, Saccharomyces cerevisiae
Free Radic Biol Med. 2023 Dec 25:S0891-5849(23)01189-9. doi: 10.1016/j.freeradbiomed.2023.12.033. Online ahead of print.ABSTRACTEcotoxicological assays have traditionally focused on the effects of chemicals at the individual level by exploiting mortality and reproduction as endpoints. Although these two parameters are ecologically relevant, they rarely provide information regarding the elemental toxic mechanisms. Obviously, the number of xenobiotics used has been rapidly increased. Thus, any established measurement that shortens the actual outcome and, simultaneously provides information about toxic mechanisms is desirable...
Source: Free Radical Biology and Medicine - December 27, 2023 Category: Biology Authors: Panwad Sillapawattana Martin Clemen Horst Gruhlke Thomas-Benjamin Seiler Prapaipat Klungsupya Wasin Charerntantanakul Source Type: research
