Dietary conjugated linoleic acid downregulates the AlCl < sub > 3 < /sub > -induced hyperactivation of compensatory and maladaptive signalling in the mouse brain cortex
Free Radic Biol Med. 2024 Mar;213:102-112. doi: 10.1016/j.freeradbiomed.2024.01.006. Epub 2024 Jan 12.ABSTRACTOxidative stress, hyperactivation of compensatory mechanisms (unfolded protein response, UPR; nuclear factor erythroid 2-related factor 2, Nrf2) and the stimulation of maladaptive response (inflammation/apoptosis) are interconnected pathogenic processes occurring during Alzheimer's disease (AD) progression. The neuroprotective ability of dietary Conjugated linoleic acid (CLAmix) in a mouse model of AlCl3-induced AD was recently described but, the effects of AlCl3 or CLAmix intake on these pathogenic processes are s...
Source: Free Radical Biology and Medicine - January 13, 2024 Category: Biology Authors: R Cuciniello D Luongo F Maurano S Crispi P Bergamo Source Type: research
Engineering M2-type macrophages with a metal polyphenol network for peripheral artery disease treatment
Free Radic Biol Med. 2024 Mar;213:138-149. doi: 10.1016/j.freeradbiomed.2024.01.014. Epub 2024 Jan 11.ABSTRACTFunctional cell treatment for critical limb ischemia is limited by cell viability loss and dysfunction resulting from a harmful ischemic microenvironment. Metal-polyphenol networks have emerged as novel cell delivery vehicles for protecting cells from the detrimental ischemic microenvironment and prolonging the survival rate of cells in the ischemic microenvironment. M2 macrophages are closely related to tissue repair, and they secrete anti-inflammatory factors that contribute to lesion repair. However, these cells...
Source: Free Radical Biology and Medicine - January 13, 2024 Category: Biology Authors: Jianwei Duan Zuoguan Chen Xiaoyu Liang Youlu Chen Huiyang Li Kaijing Liu Liang Gui Xiaoli Wang Yongjun Li Jing Yang Source Type: research
Sensitive simultaneous measurements of oxygenation and extracellular pH by EPR using a stable monophosphonated trityl radical and lithium phthalocyanine
Free Radic Biol Med. 2024 Mar;213:11-18. doi: 10.1016/j.freeradbiomed.2024.01.012. Epub 2024 Jan 11.ABSTRACTThe monitoring of acidosis and hypoxia is crucial because both factors promote cancer progression and impact the efficacy of anti-cancer treatments. A phosphonated tetrathiatriarylmethyl (pTAM) has been previously described to monitor both parameters simultaneously, but the sensitivity to tackle subtle changes in oxygenation was limited. Here, we describe an innovative approach combining the pTAM radical and lithium phthalocyanine (LiPc) crystals to provide sensitive simultaneous measurements of extracellular pH (pHe...
Source: Free Radical Biology and Medicine - January 13, 2024 Category: Biology Authors: Chloe Buyse Lionel Mignion Nicolas Joudiou Samia Melloul Benoit Driesschaert Bernard Gallez Source Type: research
Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes
In conclusion, Quer targets SLC1A5 in GC cells, inhibiting the NRF2/xCT pathway, activating the p-Camk2/p-DRP1 pathway, and accelerating iron deposition. Ultimately, Quer promotes ferroptosis in GC cells, inhibiting GC progression. Overall, our study reveals that Quer can potentially impede GC progression by targeting SLC1A5, offering novel therapeutic avenues through the modulation of ferroptosis and iron homeostasis.PMID:38190923 | DOI:10.1016/j.freeradbiomed.2024.01.002 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - January 8, 2024 Category: Biology Authors: Lixian Ding Shuwei Dang Mingjun Sun Dazhi Zhou Yanyan Sun Encheng Li Shuqi Peng Jinxing Li Guodong Li Source Type: research
Breviscapine protects against pathological cardiac hypertrophy by targeting FOXO3a-mitofusin-1 mediated mitochondrial fusion
In conclusion, nuclear FOXO3a translocation induced by BRE presents a successful therapeutic avenue for addressing cardiac hypertrophy and HF through promoting MFN-1-dependent mitochondrial fusion.PMID:38190924 | DOI:10.1016/j.freeradbiomed.2024.01.007 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - January 8, 2024 Category: Biology Authors: Xiaobing Lin Ming-Zhou Fei An-Xian Huang Liu Yang Ze-Jie Zeng Wen Gao Source Type: research
Verteporfin induces lipid peroxidation and ferroptosis in pancreatic cancer cells
In this study, we examined the effect of VP on reactive oxygen species (ROS) and lipid peroxidation in pancreatic cancer cells, by using fluorescent molecular probes and by measuring the levels of malondialdehyde, a metabolic byproduct and marker of lipid peroxidation. We found that VP causes rapid increase of both overall ROS and lipid peroxide levels, independent of light activation. These effects were not dependent on YAP, as knockdown of YAP did not cause ROS or lipid peroxidation or enhance VP-induced ROS production. Temoporfin, another photodynamic drug, did not show similar activities. In addition, VP treatment led ...
Source: Free Radical Biology and Medicine - January 6, 2024 Category: Biology Authors: Wei Zhou Adrian Lim Omer Elmadbouh Mouad Edderkaoui Arsen Osipov Angela Mathison Raul Urrutia Tao Liu Qiang Wang Stephen J Pandol Source Type: research
Verteporfin induces lipid peroxidation and ferroptosis in pancreatic cancer cells
In this study, we examined the effect of VP on reactive oxygen species (ROS) and lipid peroxidation in pancreatic cancer cells, by using fluorescent molecular probes and by measuring the levels of malondialdehyde, a metabolic byproduct and marker of lipid peroxidation. We found that VP causes rapid increase of both overall ROS and lipid peroxide levels, independent of light activation. These effects were not dependent on YAP, as knockdown of YAP did not cause ROS or lipid peroxidation or enhance VP-induced ROS production. Temoporfin, another photodynamic drug, did not show similar activities. In addition, VP treatment led ...
Source: Free Radical Biology and Medicine - January 6, 2024 Category: Biology Authors: Wei Zhou Adrian Lim Omer Elmadbouh Mouad Edderkaoui Arsen Osipov Angela Mathison Raul Urrutia Tao Liu Qiang Wang Stephen J Pandol Source Type: research
Lipid peroxidation products' role in autophagy regulation
Free Radic Biol Med. 2024 Jan 4;212:375-383. doi: 10.1016/j.freeradbiomed.2024.01.001. Online ahead of print.ABSTRACTAutophagy, which is responsible for removing damaged molecules, prevents their accumulation in cells, thus maintaining intracellular homeostasis. It is also responsible for removing the effects of oxidative stress, so its activation takes place during increased reactive oxygen species (ROS) generation and lipid peroxidation. Therefore, the aim of this review was to summarize all the available knowledge about the effect of protein modifications by lipid peroxidation products on autophagy activation and the im...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Agnieszka G ęgotek El żbieta Skrzydlewska Source Type: research
Clozapine-N-oxide protects dopaminergic neurons against rotenone-induced neurotoxicity by preventing ferritinophagy-mediated ferroptosis
This study aimed to confirm the neuroprotective effects of CLZ and CNO in a rotenone-induced mouse model and further explore the underlying mechanisms of CNO-afforded protection. Gait pattern and rotarod activity evaluations showed motor impairments in rotenone-exposed mice, with CLZ or CNO administration ameliorating behavioral deficits. Cell counts and biochemical analysis demonstrated CLZ and CNO's effectiveness in reducing rotenone-induced neurodegeneration of dopaminergic neurons in the nigrostriatal system in mice. Mechanistic investigations revealed that CNO suppressed rotenone-induced ferroptosis of dopaminergic ne...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Qingquan Sun Yan Wang Liyan Hou Sheng Li Jau-Shyong Hong Qingshan Wang Jie Zhao Source Type: research
Ascorbate and its transporter SVCT2: The dynamic duo's integrated roles in CNS neurobiology and pathophysiology
Free Radic Biol Med. 2024 Jan 3:S0891-5849(23)01202-9. doi: 10.1016/j.freeradbiomed.2023.12.040. Online ahead of print.ABSTRACTAscorbate is a small antioxidant molecule essential for the proper development and function of the brain. Ascorbate is transported into the brain and between brain cells via the Sodium vitamin C co-transporter 2 (SVCT2). This review provides an in-depth analysis of ascorbate's physiology, including how ascorbate is absorbed from food into the CNS, emphasizing cellular mechanisms of ascorbate recycling and release in different CNS compartments. Additionally, the review delves into the various functi...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Camila C Portugal Source Type: research
Lipid peroxidation products' role in autophagy regulation
Free Radic Biol Med. 2024 Jan 3:S0891-5849(24)00001-7. doi: 10.1016/j.freeradbiomed.2024.01.001. Online ahead of print.ABSTRACTAutophagy, which is responsible for removing damaged molecules, prevents their accumulation in cells, thus maintaining intracellular homeostasis. It is also responsible for removing the effects of oxidative stress, so its activation takes place during increased reactive oxygen species (ROS) generation and lipid peroxidation. Therefore, the aim of this review was to summarize all the available knowledge about the effect of protein modifications by lipid peroxidation products on autophagy activation ...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Agnieszka G ęgotek El żbieta Skrzydlewska Source Type: research
Ascorbate and its transporter SVCT2: The dynamic duo's integrated roles in CNS neurobiology and pathophysiology
Free Radic Biol Med. 2024 Jan 3:S0891-5849(23)01202-9. doi: 10.1016/j.freeradbiomed.2023.12.040. Online ahead of print.ABSTRACTAscorbate is a small antioxidant molecule essential for the proper development and function of the brain. Ascorbate is transported into the brain and between brain cells via the Sodium vitamin C co-transporter 2 (SVCT2). This review provides an in-depth analysis of ascorbate's physiology, including how ascorbate is absorbed from food into the CNS, emphasizing cellular mechanisms of ascorbate recycling and release in different CNS compartments. Additionally, the review delves into the various functi...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Camila C Portugal Source Type: research
Clozapine-N-oxide protects dopaminergic neurons against rotenone-induced neurotoxicity by preventing ferritinophagy-mediated ferroptosis
This study aimed to confirm the neuroprotective effects of CLZ and CNO in a rotenone-induced mouse model and further explore the underlying mechanisms of CNO-afforded protection. Gait pattern and rotarod activity evaluations showed motor impairments in rotenone-exposed mice, with CLZ or CNO administration ameliorating behavioral deficits. Cell counts and biochemical analysis demonstrated CLZ and CNO's effectiveness in reducing rotenone-induced neurodegeneration of dopaminergic neurons in the nigrostriatal system in mice. Mechanistic investigations revealed that CNO suppressed rotenone-induced ferroptosis of dopaminergic ne...
Source: Free Radical Biology and Medicine - January 5, 2024 Category: Biology Authors: Qingquan Sun Yan Wang Liyan Hou Sheng Li Jau-Shyong Hong Qingshan Wang Jie Zhao Source Type: research
P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability
Free Radic Biol Med. 2024 Jan 2;212:336-348. doi: 10.1016/j.freeradbiomed.2023.12.047. Online ahead of print.ABSTRACTFerroptosis is involved in the pathogenesis of osteoarthritis (OA) while suppression of chondrocyte ferroptosis has a beneficial effect on OA. However, the molecular mechanism of ferroptosis in OA remains to be elucidated. P21, an indicator of aging, has been reported to inhibit ferroptosis, but the relationship between P21 and ferroptosis in OA remains unclear. Here, we aimed to investigate the expression and function of P21 in OA chondrocytes, and the involvement of P21 in the regulation of ferroptosis in ...
Source: Free Radical Biology and Medicine - January 4, 2024 Category: Biology Authors: Zehang Zheng Xingru Shang Kai Sun Yanjun Hou Xiong Zhang Jingting Xu Haigang Liu Zhaoxuan Ruan Liangcai Hou Zhou Guo Genchun Wang Fei Xu Fengjing Guo Source Type: research
P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability
Free Radic Biol Med. 2024 Jan 2:S0891-5849(23)01209-1. doi: 10.1016/j.freeradbiomed.2023.12.047. Online ahead of print.ABSTRACTFerroptosis is involved in the pathogenesis of osteoarthritis (OA) while suppression of chondrocyte ferroptosis has a beneficial effect on OA. However, the molecular mechanism of ferroptosis in OA remains to be elucidated. P21, an indicator of aging, has been reported to inhibit ferroptosis, but the relationship between P21 and ferroptosis in OA remains unclear. Here, we aimed to investigate the expression and function of P21 in OA chondrocytes, and the involvement of P21 in the regulation of ferro...
Source: Free Radical Biology and Medicine - January 4, 2024 Category: Biology Authors: Zehang Zheng Xingru Shang Kai Sun Yanjun Hou Xiong Zhang Jingting Xu Haigang Liu Zhaoxuan Ruan Liangcai Hou Zhou Guo Genchun Wang Fei Xu Fengjing Guo Source Type: research
