Hydrogen peroxide is not generated intracellularly in human neural spheroids during ischemia-reperfusion

Free Radic Biol Med. 2023 Dec 27;212:234-240. doi: 10.1016/j.freeradbiomed.2023.12.039. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are considered a primary source of damage during ischemic stroke. However, the precise timing of ROS production (during hypoxia or reperfusion) remains unclear. Cellular 3D spheroids are often proposed as an optimal alternative to both 2D cell cultures and animal models in modeling disease conditions. Here we report live imaging of hydrogen peroxide dynamics during the acute phase of hypoxia and reperfusion in human iPSC-derived neural spheroids, stably expressing fluorescent biosensor HyPer7. Contrary to previous reports, we did not observe a hydrogen peroxide production burst neither during hypoxia nor in course of reperfusion. Our data suggest either lack of oxidative stress during ischemia-reperfusion in spheroids or existence of different mechanisms of oxidative damage.PMID:38158053 | DOI:10.1016/j.freeradbiomed.2023.12.039

Source: Free Radical Biology and Medicine - December 29, 2023 Category: Biology Authors: Veronika S Usatova Natalie M Mishina Mikhail A Berestovoy Alexander V Ivanenko David Jappy Viktoriya G Krut' Rostislav A Sokolov Aleksandr A Moshchenko Andrei Rozov Evgeny K Shevchenko Vsevolod V Belousov Source Type: research