Live-cell imaging Unveils stimulus-specific dynamics of Nrf2 activation in UV-exposed melanoma cells: Implications for antioxidant compound screening
Free Radic Biol Med. 2023 Dec 11;211:1-11. doi: 10.1016/j.freeradbiomed.2023.12.007. Online ahead of print.ABSTRACTThe transcription factor Nuclear factor e2-related factor 2 (Nrf2) is pivotal in orchestrating cellular antioxidant defense mechanisms, particularly in skin cells exposed to ultraviolet (UV) radiation and electrophilic phytochemicals. To comprehensively investigate Nrf2's role in maintaining cellular redox equilibrium following UV-induced stress, we engineered a novel Nrf2 fusion-based reporter system for real-time, live-cell quantification of Nrf2 activity in human melanoma cells. Utilizing live quantitative ...
Source: Free Radical Biology and Medicine - December 13, 2023 Category: Biology Authors: Nilar Win Htut Tasanee Onkoksoong Malinee Saelim Patipark Kueanjinda Somponnat Sampattavanich Uraiwan Panich Source Type: research
Acute and short-term beetroot juice nitrate-rich ingestion enhances cardiovascular responses following aerobic exercise in postmenopausal women with arterial hypertension: A triple-blinded randomized controlled trial
CONCLUSIONS: Acute and short-term BRJ-NO3-rich ingestion may enhance cardiovascular and autonomic behavior in response to aerobic exercise in postmenopausal women diagnosed with SAH.CLINICAL TRIAL REGISTRY NUMBER: https://clinicaltrials.gov/ct2/show/NCT05384340.PMID:38092272 | DOI:10.1016/j.freeradbiomed.2023.11.039 (Source: Free Radical Biology and Medicine)
Source: Free Radical Biology and Medicine - December 13, 2023 Category: Biology Authors: Cicero Jonas R Benjamim Leonardo Santos L da Silva Yaritza B Alves Sousa Guilherme da Silva Rodrigues Yasmim M de Moraes Pontes Macario Arosti Rebelo Leonardo da Silva Gon çalves Simone Sakagute Tavares Carolina S Guimar ães Andressa C da Silva Sobrinho Source Type: research
Inhibition of ferroptosis underlies EGCG mediated protection against Parkinson's disease in a Drosophila model
In this study, we established a PD model using PINK1 mutant Drosophila. Iron accumulation, lipid peroxidation and decreased activity of GPX, were detected in the brains of PD flies. Additionally, phenotypes of PD, including behavioral defects and dopaminergic neurons loss, were ameliorated by ferroptosis inhibitor ferrostatin-1 (Fer-1). Notably, the increased iron level, lipid peroxidation and decreased GPX activity in the brains of PD flies were relieved by EGCG. We found that EGCG exerted neuroprotection mainly by restoring iron homeostasis in the PD flies. EGCG inhibited iron influx by suppressing Malvolio (Mvl) express...
Source: Free Radical Biology and Medicine - December 13, 2023 Category: Biology Authors: Yanzhou Xia Hongyan Wang Zhongwen Xie Zhi-Hua Liu Hui-Li Wang Source Type: research
UCHL3 inhibits ferroptosis by stabilizing β-catenin and maintains stem-like properties of hepatocellular carcinoma cells
In this study, we find that UCHL3 is overexpressed in HCC tissues and correlated with β-catenin protein level. High expression of UCHL3 is associated with poor prognosis. UCHL3 knockdown markedly reduces the protein level of β-catenin in HCC cells. TOP-luciferase activity and β-catenin target genes expression are also decreased upon UCHL3 depletion. We find that the ARM domain of β-catenin is required for the interaction with UCHL3. UCHL3 increases β-catenin protein stability via removing K48-specific poly-ubiquitin chains from β-catenin protein. Furthermore, the depletion of UCHL3 induces ferroptosis and hinders the...
Source: Free Radical Biology and Medicine - December 13, 2023 Category: Biology Authors: Guo Long Zheyu Wu Dong Wang Xingyu Mi Kuan Hu Ledu Zhou Jianing Tang Source Type: research
"ATAD3C regulates ATAD3A assembly and function in the mitochondrial membrane"
Free Radic Biol Med. 2023 Dec 11:S0891-5849(23)01151-6. doi: 10.1016/j.freeradbiomed.2023.12.006. Online ahead of print.ABSTRACTMitochondrial ATAD3A is an ATPase Associated with diverse cellular Activities (AAA) domain containing enzyme, involved in the structural organization of the inner mitochondrial membrane and of increasing importance in childhood disease. In humans, two ATAD3A paralogs arose by gene duplication during evolution: ATAD3B and ATAD3C. Here we investigate the cellular activities of the ATAD3C paralog that has been considered a pseudogene. We detected unique ATAD3C peptides in HEK 293T cells, with express...
Source: Free Radical Biology and Medicine - December 13, 2023 Category: Biology Authors: Paula Gaud ó Elena de Tom ás-Mateo Nuria Garrido-P érez Alfredo Santana Eduardo Ruiz-Pesini Julio Montoya Pilar Bayona-Bafaluy Source Type: research
