Acknowledgement to Referees
Cells Tissues Organs 2021;210:391 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - December 9, 2021 Category: Cytology Source Type: research
An electrochemical protocol for CRISPR-mediated gene-editing of sheep embryonic fibroblast cells
In conclusion, we developed an electrotransfection protocol using the OptiMEM-GlutaMAX medium supplemented with 3 % DMSO for sheep EF cells. The electroporation method can be used for cell-mediated gene-editing in sheep. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - November 25, 2021 Category: Cytology Source Type: research
Lentivirus-shRNA mediated prolyl hydroxylase 2 knockdown increases HIF-1 α and inhibits nucleus pulposus cells degeneration
In conclusion, interference with PHD2 can upregulate the expression of HIF-1α, accelerate anabolism, reduce catabolism, inhibit apoptosis of nucleus pulposus cells, and then these can inhibit degeneration of nucleus pulposus cells. Our results can provide an effective therapeutic target in intervertebral discs during intervertebral disc degeneration and this may have important clinical significance. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - November 15, 2021 Category: Cytology Source Type: research
Biographies of Contributors
Cells Tissues Organs (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - November 10, 2021 Category: Cytology Source Type: research
The effect of exosomes derived from unrestricted somatic stem cells on murine model of sepsis
In conclusion, we reported that direct injection of USSC- derived exosomes can be one of the important methods for the treatment of various aspects of sepsis due to their immunomodulatory properties. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - November 8, 2021 Category: Cytology Source Type: research
Downregulation of Pinkbar/pAKT and MMP2/MMP9 Expression in MDA-MB-231 Breast Cancer Cells as Potential Targets in Cancer Therapy by hAMSCs Secretome
Breast cancer is one of the leading causes of cancer-related deaths among women worldwide. Cancer therapy based on stem cells is considered as a novel and promising platform. In the present study, we explored the therapeutic effects of human amniotic mesenchymal stromal cells (hAMSCs) through Pinkbar (planar intestinal- and kidney-specific BAR domain protein), pAKT, and matrix metalloproteinases including MMP2 and MMP9 on MDA-MB-231 breast cancer cells. For this purpose, we employed a co-culture system using Transwell 6-well plates with a pore size of 0.4 μm. After 72 h, the hAMSCs-treated MDA-MB-231 breast cancer cells, ...
Source: Cells Tissues Organs - October 25, 2021 Category: Cytology Source Type: research
Down regulation of Pinkbar/pAKT and MMP2/MMP9 expression in MDA-MB-231 breast cancer cells as potential targets in cancer therapy by hAMSCs secretome
Breast cancer (BC) is one of the most causes of cancer-related death among women worldwide. Cancer therapy based on stem cells was considered as a novel and promising platform. In present study, we explored the therapeutic effects of human amniotic mesenchymal stromal cells (hAMSCs) through Pinkbar (planar intestinal-and kidney-specific BAR domain protein), pAKT, and matrix metalloproteinases including MMP2, MMP9 on MDA-MB-231 breast cancer cells. To do so, we employed a co-culture system using 6 well plates transwell with a diameter of 0.4 μm pore sized. After 72h hAMSCs-treated MDA-MB-231 breast cancer cells, the expres...
Source: Cells Tissues Organs - October 25, 2021 Category: Cytology Source Type: research
EphB3 as a potential mediator of developmental and reparative osteogenesis
The ephrin-B family of membrane-bound ligands is involved in skeletal patterning, osteogenesis, and bone homeostasis. Yet, despite the increasing collection of data affirming their importance in bone, the Eph tyrosine kinases that serve as the receptors for these ephrins in osteoblast stem cell niches remain unidentified. Here we report the expression of EphB3 at sites of bone growth in the embryo, especially at the calvaria suture fronts, periosteum, chondrocytes and trabeculae of developing long bones. Strong EphB3 expression persisted in the adult calvarial sutures and in the proliferative chondrocytes of long bones, bo...
Source: Cells Tissues Organs - October 25, 2021 Category: Cytology Source Type: research
Hemolymph Node – An Immunomorphological Organ: Modeling the Hemolymph Node by Allografting Renal Tissue in the Rat
In this study, the model of the HLN was established by transferring allogeneic renal tissue in the rat. Intrasinusoidal erythrocytes of the node were the component for producing a red macroscopic appearance, while macrophage-erythrocyte-lymphocyte rosettes were the major immunomorphological changes, reflecting the immune activity against the invasion of the allogeneic tissue within the node. Therefore, the HLN is an immunomorphological organ. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - October 25, 2021 Category: Cytology Source Type: research
Haemolymph node – an immunomorpholoigcal organ: modeling the haemolymph node by allografting renal tissue in the rat
In this study, the model of the haemolymph node was established by transferring the allogeneic renal tissue in the rat. Intrasinusoidal erythrocytes of the node were the component for producing a red macroscopic appearance, while macrophage-erythrocyte-lymphocyte rosettes were the major immunomorphological changes, which reflects the immune activity against the invasion of the allogeneic tissue within the node. Therefore the haemolymph node is an immunomorphological organ. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - October 25, 2021 Category: Cytology Source Type: research
Keratin 17 as a marker of UVB-induced stress in human epidermis and modulation by Vitis vinifera extract
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - October 13, 2021 Category: Cytology Source Type: research
Responsiveness of subcutaneous fat, intermuscular fat, and muscle anatomical cross-sectional area of the thigh to longitudinal body weight loss and gain – Data from the Osteoarthritis Initiative (OAI)
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - October 7, 2021 Category: Cytology Source Type: research
LncRNA HOTAIR promotes chemoresistance by facilitating epithelial to mesenchymal transition through miR-29b/PTEN/PI3K signaling in cervical cancer
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - September 27, 2021 Category: Cytology Source Type: research
Chimyl alcohol exhibits proinflammatory activity in vivo and in vitro
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - September 27, 2021 Category: Cytology Source Type: research
Intermittent Fasting Attenuates High-Fat Diet-Induced Cerebellar Changes in Rats: Involvement of TNF- α, Autophagy, and Oxidative Stress
This study examines the effect of IF on the cerebellum of HFD-fed rats. Male Wister Albino rats (n = 16) were fed HFD for 16 weeks (HFD group); half of them were subjected to IF alternating with HFD for 6 weeks starting at the 11th week till the end of the experiment (fasting + HFD group). The control group of rats (n = 8) was kept on a basal diet. The animals were euthanized after 16 weeks. Their tissue was harvested and processed for morphology using Hamp;E, cresyl violet, and luxol fast stains, and immunohistochemical staining was carried out for inflammatory marker (TNF- α), gliosis marker (GFAP), and autophagy marker...
Source: Cells Tissues Organs - September 22, 2021 Category: Cytology Source Type: research
