Organoids in the clinic: a systematic review of outcomes
This article presents a systematic review and appraisal of the published literature in line with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines together with a synopsis of recent relevant reviews. Surprisingly, no randomized controlled trials have reported clinical outcomes with any types of organoids. We found very few ongoing and registered studies that may provide clinically relevant results within this decade. Our screening and interpretation of the literature, including review articles, indicate a focus on technical and pre-clinical aspects of organoid research. This is the ...
Source: Cells Tissues Organs - September 28, 2022 Category: Cytology Source Type: research
Hepatocyte Growth Factor Promotes Differentiation Potential and Stress Response of Human Stem Cells from Apical Papilla
In this study, HGF was transgenic expressed in SCAP, and it was found that HGF enhanced osteo/dentinogenic differentiation capacity of SCAP compared with those non-treated control in an ectopic mineralization model. Moreover, HGF reduced apoptosis of SCAP under both normoxia and hypoxia condition, whereas combination of HGF and hypoxia exposure had inhibitory effects on cell proliferation during an eight-day in vitro culture period. Transcriptome analysis further revealed that suppressed cell cycle progression and activated BMP/ TGFβ, Hedge hog, WNT, FGF, HOX and other morphogen family members upon HGF overexpression, whi...
Source: Cells Tissues Organs - September 28, 2022 Category: Cytology Source Type: research
Altered expression of pulmonary epithelial cell markers in fetal and adult mice generated by in vitro embryo culture and embryo transfer
Lung development is impaired in mice generated through transfer of in vitro-derived blastocysts. The main objective of the current study was to determine if the composition of epithelial cells in the fetal and adult lung tissue is altered in mice generated through transfer of in vitro-derived blastocysts. The study comprised of two experimental (EGs) and two control (CGs) groups. Fetuses (18.5 d.p.c.) and adult mice (8-week-old) of the EGs (EGfetus , n=18, EGadult, n=15) were produced by the transfer of day-5 F2 blastocysts to pseudo-pregnant females. F2 fetuses and adult mice derived from naturally-ovulating females serve...
Source: Cells Tissues Organs - September 14, 2022 Category: Cytology Source Type: research
Author Index
Cells Tissues Organs 2022;211:537 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - September 2, 2022 Category: Cytology Source Type: research
Artificial microvesicles: new perspective on healing tendon wounds
Tendons have a limited capacity to repair both naturally and following clinical interventions. Damaged tissue often presents with structural and functional differences, adversely affecting animal performance, mobility, health and welfare. Advances in cell therapies have started to overcome some of these issues, however complications such as the formation of ectopic bone remain a complication of this technique. Regenerative medicine is therefore looking towards future therapies such as the introduction of microvesicles (MVs) derived from stem cells (SCs). The aim of the present study was to assess the characteristics of art...
Source: Cells Tissues Organs - September 1, 2022 Category: Cytology Source Type: research
Molecular characterization and differentiation of mesenchymal progenitor cells from human rheumatoid arthritis cartilage
The presence of mesenchymal progenitor cells (MPCs) in rheumatoid arthritis (RA) articular cartilage is sparsely investigated largely owing to the persistent pathogenic disease condition and lack of specific biomarkers. Considering the recent advancements for potential cell-based therapies in immunomodulatory diseases, such as RA, this in vitro study was aimed at investigating the cellular, molecular and differentiation characteristics of human RA cartilage-derived MPCs. Articular cartilage fragments from RA patients were obtained for the isolation and characterization of MPCs on their cellular and biological properties, c...
Source: Cells Tissues Organs - August 26, 2022 Category: Cytology Source Type: research
Apoptosis-Decellularized Peripheral Nerve Scaffold Allows Regeneration across Nerve Gap
This study examines the utility of apoptosis-decellularized peripheral nerve scaffolds compared to detergent-decellularized peripheral nerve scaffolds an d isograft controls in a rat nerve gap model. Results indicate that, at 8 weeks post-injury, apoptosis-decellularized peripheral nerve scaffolds perform similarly to detergent-decellularized and isograft controls in both functional (muscle weight recovery, gait analysis) and histological measures (n eurofilament staining, macrophage infiltration). These new apoptosis-decellularized scaffolds hold great promise to provide a less expensive scaffold for nerve injury repair, ...
Source: Cells Tissues Organs - August 26, 2022 Category: Cytology Source Type: research
A Multistep Tumor Growth Model of High Grade Serous Ovarian Carcinoma Identifies Hypoxia Associated Signatures
High-grade serous ovarian carcinoma (HGSC) is associated with late-stage disease presentation and poor prognosis, with limited understanding of early transformation events. Our study presents a comprehensive analysis of tumor progression and organ-specific metastatic dissemination to identify hypoxia-associated molecular, cellular, and histological alterations during HGSC tumor growth. HE staining and subsequent histological assessment of tumor volume-based categories revealed recapitulation of numerous clinical features, including the prevalence of>0.0625 ≤0.5cm3 volume tumors and metastatic spread by orthotopic xenogra...
Source: Cells Tissues Organs - August 15, 2022 Category: Cytology Source Type: research
