Regulation of alternative splicing during epithelial-mesenchymal transition
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 8, 2021 Category: Cytology Source Type: research
Death-associated Protein Kinase 1 Regulates Oxidative Stress In Cardiac Ischemia Reperfusion Injury
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 8, 2021 Category: Cytology Source Type: research
The biography of specimen "09.04.1954, 3.4 mm" from the "Blechschmidt Collection of Human Embryos" at G öttingen University. With a special focus on the production and usage of enlarged 3D replicas of embryos in the anatomical research on human embryos.
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 8, 2021 Category: Cytology Source Type: research
Age-Related Alterations of Proteins in Albino Wistar Rat Retina
Imbalance of homeostasis causes permanent changes in the body with time. The central nervous system is especially prone to these changes since it possesses limited regenerative capacity. In the retina, neurons are damaged during the aging process, and this eventually leads to deterioration of vision. In our 2-year-long study, we examined genetically closely related rat individuals to disclose the hidden retinal causes of age-associated visual dysfunction. Morphometric analysis showed significant reduction of the retina thickness with aging, particularly that of the inner plexiform layer. To reveal changes between the age g...
Source: Cells Tissues Organs - July 2, 2021 Category: Cytology Source Type: research
Conservation of Epithelial-to-Mesenchymal Transition Process in Neural Crest Cells and Metastatic Cancer
Epithelial to mesenchymal transition (EMT) is a highly conserved cellular process in several species, from worms to humans. EMT plays a fundamental role in early embryogenesis, wound healing, and cancer metastasis. For neural crest cell (NCC) development, EMT typically results in forming a migratory and potent cell population that generates a wide variety of cell and tissue, including cartilage, bone, connective tissue, endocrine cells, neurons, and glia amongst many others. The degree of conservation between the signaling pathways that regulate EMT during development and metastatic cancer (MC) has not been fully establish...
Source: Cells Tissues Organs - July 2, 2021 Category: Cytology Source Type: research
New Endeavors of (Micro)Tissue Engineering: Cells Tissues Organs on-Chip and Communication Thereof
The development of new therapies is tremendously hampered by the insufficient availability of human model systems suitable for preclinical research on disease target identification, drug efficacy, and toxicity. Thus, drug failures in clinical trials are too common and too costly. Animal models or standard 2D in vitro tissue cultures, regardless of whether they are human based, are regularly not representative of specific human responses. Approaching near human tissues and organs test systems is the key goal of organs-on-chips (OoC) technology. This technology is currently showing its potential to reduce both drug developme...
Source: Cells Tissues Organs - July 1, 2021 Category: Cytology Source Type: research
Axolotls ’ and Mice’s Oral-Maxillofacial Trephining Wounds Heal Differently
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 1, 2021 Category: Cytology Source Type: research
Engineering Fiber-Based Nervous Tissue Constructs for Axon Regeneration
This study also evaluated the response of dorsal root ganglion neurons from adult rats to provide data more relevant to axon regenerative potential in nerve repair. We found that ECM treatments on fibers enhanced the regeneration of adult axons indicating that both the physical and biochemical presentation of the fibers are essential for enhancing axon guidance and growth.Cells Tissues Organs (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 1, 2021 Category: Cytology Source Type: research
Basis of Image Analysis for Evaluating Cell Biomaterial Interaction Using Brightfield Microscopy
We present the major imaging acquisition techniques that enable point-of-care testing when incorporated with microfluidic cells, discuss the constraints enforced by the geometry of the system and the material that is analyzed, and the challenges that rise in the image analysis when unstained cell imaging is employed. Emerging techniques such as utilization of machine learning and cell-specific pattern recognition algorithms and potential future directions are discussed. Automation and optimization of biomaterial assessment can facilitate the discovery of novel biomaterials together with making the validation of biomedical ...
Source: Cells Tissues Organs - June 29, 2021 Category: Cytology Source Type: research
Establishment of a Systemic Inflammatory Response Syndrome Model and Evaluation of the Efficacy of Umbilical Cord Mesenchymal Stem Cell Transplantation
Based on the characteristics of modern weapon injury, a repetitive model of traumatic systemic inflammatory response syndrome (SIRS) and an evaluation system were established. The models were treated with GFP-labeled tree shrew umbilical cord mesenchymal stem cells (UCMSCs). Forty out of 50 tree shrews were used to make a unilateral femoral comminuted fracture. Lipopolysaccharide was injected intravenously to create a traumatic SIRS model. The other 10 shrews were used as normal controls. After the model was established for 10 days, 20 tree shrews were injected intravenously with GFP-labeled UCMSCs, and 18 tree shrews were...
Source: Cells Tissues Organs - June 28, 2021 Category: Cytology Source Type: research
Biomaterial Strategies to Bolster Neural Stem Cell-Mediated Repair of the Central Nervous System
Stem cell therapies have the potential to not only repair, but to regenerate tissue of the central nervous system (CNS). Recent studies demonstrate that transplanted stem cells can differentiate into neurons and integrate with the intact circuitry after traumatic injury. Unfortunately, the positive findings described in rodent models have not been replicated in clinical trials, where the burden to maintain the cell viability necessary for tissue repair becomes more challenging. Low transplant survival remains the greatest barrier to stem cell-mediated repair of the CNS, often with fewer than 1 –2% of the transplanted cel...
Source: Cells Tissues Organs - June 11, 2021 Category: Cytology Source Type: research
Sex Differences in the Temporalis Tendon-Aponeurotic Complex: An in vivo MRI Macroscopic Analysis in Children and Adolescents
The tendon-aponeurosis complex (TAC) of the temporalis dissipates forces produced during function. Abnormally reduced temporalis TACs have been found in individuals with chronic muscular temporomandibular disorders – a painful musculoskeletal condition that is more frequent in women than men. Whether there are sex differences in the temporalis TAC in healthy individuals is currently unknown. Here, we characterized and measured the temporalis TAC in healthy male and female young individuals between 5 and 15 y ears old to determine whether the volume of the temporalis TAC and the TAC-to-muscle ratio are different between m...
Source: Cells Tissues Organs - June 4, 2021 Category: Cytology Source Type: research
Advances in Extracellular Matrix-Mimetic Hydrogels to Guide Stem Cell Fate
In the fields of regenerative medicine and tissue engineering, stem cells offer vast potential for treating or replacing diseased and damaged tissue. Much progress has been made in understanding stem cell biology, yielding protocols for directing stem cell differentiation toward the cell type of interest for a specific application. One particularly interesting and powerful signaling cue is the extracellular matrix (ECM) surrounding stem cells, a network of biopolymers that, along with cells, makes up what we define as a tissue. The composition, structure, biochemical features, and mechanical properties of the ECM are varie...
Source: Cells Tissues Organs - June 3, 2021 Category: Cytology Source Type: research
Relationship between Stemness, Reactive Oxygen Species, and Epithelial-to-Mesenchymal Transition in Model Circulating Tumor Cells
Most cancer deaths are caused by secondary metastasized tumors. The cells that spread these tumors are known as circulating tumor cells (CTCs). They exist in a dynamic environment, including exposure to fluid shear stress (FSS) that makes them susceptible to reactive oxygen species (ROS) generation. There are questions about the similarities of CTCs to cancer stem cells (CSCs) and whether the stem cell-like characteristics of CTCs allow them to proliferate and spread despite the biophysical obstacles during the metastatic process. One of those qualities is the ability to undergo the epithelial-to-mesenchymal transition (EM...
Source: Cells Tissues Organs - June 2, 2021 Category: Cytology Source Type: research
Self-Standing Photo-Crosslinked Hydrogel Construct: in vitro Microphysiological Vascular Model
Modeling of the human vascular microphysiological system (MPS) has gained attention due to precise prediction of drug response and toxicity during drug screening process. Developing a physiologically equivalent vascular MPS still remains complex as it demands the recapitulation of dynamic structural and biological microenvironment similar to native vasculature. Hence, an ideal MPS would involve developing perfusable 3D in vitro models with multilayered human vascular cells encapsulated in a matrix to regulate the vascular tone resembling the native. Several attempts to model such anatomically accurate physiological and pat...
Source: Cells Tissues Organs - May 31, 2021 Category: Cytology Source Type: research
