A Biomaterial Model to Assess the Effects of Age in Vascularization
(Source: Cells Tissues Organs)
Source: Cells Tissues Organs - March 4, 2022 Category: Cytology Source Type: research
Three-dimensional interactive graphical model of the hindlimb muscles of the rat
Many questions in human movement sciences are addressed by exploiting the advantages of animal models. However, a 3D model of the musculoskeletal system of the frequently used rat model that includes a sufficient level of detail does not exist. Therefore, the aim of the present work was to develop an freely accessible 3D model of the rat hindlimb. Using the anatomical data of the Wistar rat (Mus norveqicus albinus) published by Green [Greene, 1935], a 3D representation of 34 muscles of the hindlimb was drawn. Two models were created, one using muscle like appearances and one using different colors. Each muscle can be viewe...
Source: Cells Tissues Organs - February 24, 2022 Category: Cytology Source Type: research
Brain cancer cell-derived matrices and effects on astrocyte migration
We report the use of macromolecular crowding (MMC) alone as an effective strategy to generate brain cancer cell-derived matrices for downstream analyses and cell migration studies. We cultured the mouse glioblastoma cell line GL261 for 1 week in the presence of three previously-reported MMC agents (carrageenan, Ficoll 70/400, and hyaluronic acid). We measured the resulting deposition of collagens and sulfated glycosaminoglycans using quantitative assays, as well as other matrix components by immunostaining. Both carrageenan and Ficoll promoted significantly more accumulation of total collagen content, sulfated glycosaminog...
Source: Cells Tissues Organs - February 15, 2022 Category: Cytology Source Type: research
Matrix Bound Nanovesicles: What are they and what do they do?
Over the past 50 years, several different types of extracellular vesicles have been discovered including: exosomes, microvesicles, and matrix vesicles. These vesicles are secreted by cells for specific purposes and contain cargo such as micro-RNA, cytokines, and lipids. A novel extracellular vesicle, the matrix bound nanovesicle (MBV), has been recently discovered. The MBV is similar to the microvesicle however, it is attached to the extracellular matrix, instead of being secreted. This review compares MBVs to other types of extracellular vesicles to try and better understand their origin and function. Further, this review...
Source: Cells Tissues Organs - February 15, 2022 Category: Cytology Source Type: research
Silk-based matrices and c-Kit positive cardiac progenitor cells for a cellularized silk fibroin scaffold: study of an in vivo model
In conclusion, c-kit-positive CPCs, although expressing a good level of cardiac diffe rentiation markers in vitro with or without fibroin meshes, are not suitable for an in vivo model of cardiac organoids because they are degraded by a T-cell-mediated immune response. Even scaffolds, which may preserve the survival of these cells in vivo, also induced a host response. However, among the tested scaffolds, the electrospun meshes (F-scaffold) induced a lower response compared to all the other tested structures. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - February 14, 2022 Category: Cytology Source Type: research
Role and mechanism of miR-181a-5p in mice with chronic obstructive pulmonary disease by regulating HMGB1 and the NF- κB pathway
This study explored the mechanism of miR-181a-5p in the inflammatory response in COPD mice. COPD mouse models were established by cigarette smoke (CS) exposure following pretreatment with recombinant adeno-associated virus (rAAv)-miR-181a-5p, si-HMGB1 (high mobility group box 1), and NF- κB pathway inhibitor PDTC, respectively. Pathological changes of lung tissues were determined by HE staining. BALF was collected to count total cells, neutrophils and lymphocytes using a Countess II automatic cell counter. Expressions of NE and inflammatory factors (TNF-α, IL-6, IL-8 and IFN-γ) w ere detected by ELISA. Binding relations...
Source: Cells Tissues Organs - January 24, 2022 Category: Cytology Source Type: research
Synthetic Small Molecules Induce Insulin Secretion and Pancreatic Beta-Cell-Specific Gene Expression
In this study, a family of new SMs that are structurally similar to glinides was synthesized through 3 different synthetic methods and categorized into 3 categories (C1 –C3). Then, these novel SMs were characterized by testing their effects on cell viability, pβC-specific gene expression, and insulin secretion in MIN6 in 4 different concentrations and at 3 time points (24, 48, and 72 h). According to our results, SMs of C1 (1j, 1k, and 1l) and 2 SMs of C3 (1f, 1 i), at 200 μM concentration, were able to increase the expression levels ofPdx1,Neurod1,Ngn3, andIns1 as well as the insulin secretion after 24 h. However, C2 ...
Source: Cells Tissues Organs - January 24, 2022 Category: Cytology Source Type: research
Synthetic Small Molecules to Induce Insulin Secretion and Pancreatic Beta Cell Specific Gene Expression
In this study, a family of new SMs that are structurally similar to glinides was synthesized through three different synthetic methods and categorized into three categories (C1-C3). Then, these novel SMs were characterized by testing their effects on cell viability, pβC s-specific gene expression, and insulin secretion of MIN6 in four different concentrations and three time points. According to our results, SMs of C1 (1j, 1k, and 1l) and two SMs of C3 (1f, 1I), at 200 μM concentration, were able to increase the expression levels of Pdx1, Neurod1, Ngn3, and Ins1 as well as the insulin secretion after 24 hours. However, C2...
Source: Cells Tissues Organs - January 24, 2022 Category: Cytology Source Type: research
Controlling Morphology and Functions of Cardiac Organoids by Two-Dimensional Geometrical Templates
This study will shed a light on “organogenesis-by-design” by increasing the intricacy of starting templates from external geometrical cues to improve the organoid morphogenesis and functionality. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - January 10, 2022 Category: Cytology Source Type: research
Rapid Prototypable Biomimetic Peristalsis Bioreactor Capable of Concurrent Shear and Multi-axial Strain
Peristalsis is a nuanced mechanical stimulus comprised of multi-axial strain (radial and axial strain) and shear stress. Forces associated with peristalsis regulate diverse biological functions including digestion, reproductive function, and urine dynamics. Given the central role peristalsis plays in physiology and pathophysiology, we were motivated to design a bioreactor capable of holistically mimicking peristalsis. We engineered a novel rotating screw-drive based design combined with a peristaltic pump, in order to deliver multiaxial strain and concurrent shear stress to a biocompatible polydimethylsiloxane (PDMS) membr...
Source: Cells Tissues Organs - January 10, 2022 Category: Cytology Source Type: research
Crosslinked Layered Surfaces of Heparin and Poly(L-Lysine) Enhance Mesenchymal Stromal Cell Behavior in the Presence of Soluble Interferon Gamma
This study suggests that crosslinked (HEP/PLL) films can modulate hMSCs response to soluble factors, which may improve hMSCs-based therapies aimed at treating several immune disea ses. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - December 22, 2021 Category: Cytology Source Type: research
Crosslinked layered surfaces of heparin and poly(L-lysine) enhance mesenchymal stromal cells behavior in the presence of soluble interferon gamma
This study suggests that crosslinked (HEP/PLL) films can modulate hMSCs response to soluble factors, which may improve hMSCs-based therapie s aimed at treating several immune diseases. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - December 22, 2021 Category: Cytology Source Type: research
Decellularized Extracellular Matrix Powder Accelerates Metabolic Maturation at Early Stages of Cardiac Differentiation in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes
During fetal development, cardiomyocytes switch from glycolysis to oxidative metabolism to sustain the energy requirements of functional cells. State-of-the-art cardiac differentiation protocols yield phenotypically immature cardiomyocytes, and common methods to improve metabolic maturation require multistep protocols to induce maturation only after cardiac specification is completed. Here, we describe a maturation method using ventricle-derived decellularized extracellular matrix (dECM) that promoted early-stage metabolic maturation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). Chemi...
Source: Cells Tissues Organs - December 21, 2021 Category: Cytology Source Type: research
Immunofluorescence studies on the expression of the SARS-CoV-2 receptors in human term placenta
Until September 2021, the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2; COVID-19) pandemic caused over 217 million infections and over 4.5 million deaths. In pregnant women the risk factors for the need of intensive care treatment are generally the same as in the overall population. Of note, COVID-19+ women deliver earlier than COVID-19- women, and the risk for severe neonatal and perinatal morbidity and mortality is significantly higher. The probability and pathways of vertical transmission of the virus from the pregnant woman to the fetus are highly controversial. Recent data have shown that 54 (13%) of 41...
Source: Cells Tissues Organs - December 16, 2021 Category: Cytology Source Type: research
Contents Vol. 210, 2021
Cells Tissues Organs 2021;210:I –IV (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - December 9, 2021 Category: Cytology Source Type: research
