Effect of Cell Derived Matrices on growth and differentiation of human Wharton ’s jelly derived Mesenchymal Stem Cells
In conclusion, the present study revea ls that the WJ-MSCs cultured on WJ-MSC-CDM may augment osteogenic and adipogenic differentiation. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 29, 2022 Category: Cytology Source Type: research
Aneuploidy rate and Stemness in Low-level Mosaic Human Embryonic Stem Cells in the Presence/Absence of Bortezomib, Paclitaxel and Lapatinib
Human embryonic stem cells (hESCs) are predisposed to aneuploidy through continual passages. Some reports indicate more sensitivity of aneuploid hESCs cells to anticancer drugs. The present study was designed to investigate the cytotoxicity of three anticancer drugs (including bortezomib, paclitaxel and lapatinib) and their effect on aneuploidy rate in hESCs.
To create a low-level mosaic cell line, normal hESCs (80%) and trisomic hESCs for chromosomes 12 and 17 (20%) were mixed. The effect of the 3 mentioned anticancer drugs on the chromosomal status was assessed by metaphase spread analysis after selection of the nontoxi...
Source: Cells Tissues Organs - July 28, 2022 Category: Cytology Source Type: research
Mesenchymal stromal cell-secretome for therapeutic application in skin wound healing: a systematic review of preclinical studies
In conclusion, despite the need for alignment of methodological protocols and transparent reports in future studies, results show that the secretome of MSCs from different tissue sources corresponds to a promising tool of regenerative medicine for the treatment of skin wounds. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 22, 2022 Category: Cytology Source Type: research
Strategies and challenges in the treatment of dental enamel
This article aims to discuss current attempts to regenerate enamel using synthetic materials methods, recent advances in enamel tissue engineering, and the prospects of enamel biomimetics to find unique insights into future possibilities for repairing enamel tissue, perhaps the most fascinating of all tooth tissues. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - July 1, 2022 Category: Cytology Source Type: research
Circ_0062582 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro by elevating SMAD5 expression through sponging miR-197-3p
In conclusion, circ_0062582 sponged miR-197-3p to elevate SMAD5 expression, thereby inducing hBMSC proliferation and osteogenic differentiation in vitro. (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - June 28, 2022 Category: Cytology Source Type: research
Author Index
Cells Tissues Organs 2022;211:360 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - June 21, 2022 Category: Cytology Source Type: research
Prelims
Cells Tissues Organs 2022;211:263 –266 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - June 21, 2022 Category: Cytology Source Type: research
miR-107 attenuates sepsis-induced myocardial injury by targeting PTEN and activating the PI3K/AKT signaling pathway
This study investigated the mechanism of miR-107 on sepsis-induced myocardial injury. Sepsis rat models were established by cecal ligation and puncture, and the cell model was established using lipopolysaccharide (LPS)-induced cardiomyocytes. Cardiac function indexes of rats were measured using echocardiography. Pathological changes in the rat myocardium were observed using histological staining. Expressions of miR-107 in the serum of rats and cardiomyocyte were detected after the treatment of miR-107 mimic or/and pcDNA3.1-PTEN, followed by assessment of cell cycle, proliferation, and apoptosis. Binding sites of miR-107 an...
Source: Cells Tissues Organs - June 17, 2022 Category: Cytology Source Type: research
miR-181a-5p restrains non-small cell lung cancer cell invasion and migration by regulating the GTSE1/p53/NF- κB axis
This study investigated the role of miR-181a-5p in lung cancer. Expression patterns of miR-181a-5p and GTSE1 in the human NSCLC cell line A549 and normal lung epithelial cell line BASE-2B were detected. miR-181a-5p mimic was delivered into A549 cells utilizing Lipofectamine 2000 to overexpress miR-181a-5p, followed by analysis of cell viability, proliferation, apoptosis, invasion, and migration. GTSE1 (G2 and S phase-expressed-1) was predicted as the downstream target gene of miR-181a-5p using bioinformatics analysis software. Targeting relationship between miR-181a-5p and GTSE1 was validated via dual-luciferase assay, RIP...
Source: Cells Tissues Organs - June 14, 2022 Category: Cytology Source Type: research
Erratum
Cells Tissues Organs (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - June 10, 2022 Category: Cytology Source Type: research
Cadherin-11 and its role in tissue fibrosis
Fibrosis is the excessive deposition of extracellular matrix that results from chronic inflammation and injury, leading the loss of tissue integrity and function. Cadherins are important adhesion molecules that classically mediate calcium-dependent cell-to-cell adhesion and play important roles in tissue development and cellular migration, but likely have functions beyond these important roles. Cadherin-11 (CDH11), a member of the cadherin family, has been implicated in several pathological processes including cancer. More recent evidence suggests that CDH11 is a central mediator of tissue fibrosis. CDH11 expression is inc...
Source: Cells Tissues Organs - June 3, 2022 Category: Cytology Source Type: research
Influence of wound fluid on the transdifferentiation of human mesenchymal bone marrow stem cells into cancer-associated fibroblasts
Cancer-associated fibroblasts (CAF) in the tumor microenvironment have a decisive influence on tumor growth and metastatic behavior. The cellular origins as well as the stimuli leading to CAF formation are heterogenous, impeding a precise characterization. Aim of this study was to analyze the influence of cytokines secreted in the process of wound healing, tumor cell-associated paracrine-secreted factors, and direct cell-cell contact on the expression of the CAF-associated markers fibroblast activation protein (FAP), α-smooth muscle actin (α-SMA), thrombospondin-1 (THBS1) and tenascin-c (TNC) by rt-PCR in mesenchymal ste...
Source: Cells Tissues Organs - June 3, 2022 Category: Cytology Source Type: research
Improving fat transplantation survival and vascularization with adenovirus E4+ endothelial cell-assisted lipotransfer
Autologous fat transplantation is plagued by an unpredictable and often significant degree of graft loss. AdE4+ endothelial cells (ECs), are human endothelial cells that have been transduced with the E4ORF1 region of human adenovirus Type 5, resulting in long-term preservation of EC proliferation and angiogenic capability without immortalization. We hypothesized that AdE4+ EC-enriched fat grafts would demonstrate improved volume retention secondary to enhanced angiogenesis. Three experimental groups were prepared by admixing 400 µL of patient lipoaspirate with 100µL of AdE4+ EC suspensions (“high” AdE4+ EC concentrat...
Source: Cells Tissues Organs - May 31, 2022 Category: Cytology Source Type: research
Acute Response of Engineered Cardiac Tissue to Pressure and Stretch
This study focused on creating a system to subject engineered cardiac tissue to either pressure or stretch stimuli in isolation and the subsequent evaluation of acute tissue remodeling. We developed a cardiac tissue chip containing three-dimensional (3-D) cell-laden hydrogel constructs and cultured them within systems where we could expose them to either pressure changes or volume changes as seen in the left ventricle. Acute cellular remodeling with each condition was qualitatively and quantitatively assessed using histology, immunohistochemistry, gene expression studies, and soluble factor analysis. Using our unique model...
Source: Cells Tissues Organs - May 31, 2022 Category: Cytology Source Type: research
