Author Index
Cells Tissues Organs 2022;211:754 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - January 4, 2023 Category: Cytology Source Type: research
Prelims
Cells Tissues Organs 2022;211:631 –634 (Source: Cells Tissues Organs)
Source: Cells Tissues Organs - January 4, 2023 Category: Cytology Source Type: research
Nuclear biophysical changes during human melanoma plasticity
Tumor plasticity is an emerging property of tumor cells which allows them to change their phenotype in dependence on the environment. The epithelial-mesenchymal transition plays a crucial role in helping cells to acquire a more aggressive phenotype when they are in the mesenchymal state. Herein we investigated the biophysical changes occurring during phenotypic switching in human melanoma cells considering the blebbines of the nuclei, their stiffness and the involvement of polycombs with lamins. We show that the formation of cellular heterogeneity involves many crucial nuclear changes including the interaction between diff...
Source: Cells Tissues Organs - December 12, 2022 Category: Cytology Source Type: research
Prediction of Golgi Polarity in Collectively Migrating Epithelial Cells Using Graph Neural Network
In the stationary epithelium, the Golgi apparatus assumes an apical position, above the cell nucleus. However, during wound healing and morphogenesis, as the epithelial cells starts migrating, it relocalizes closer to the basal plane. On this plane, the position of Golgi with respect to the cell nucleus defines the organizational polarity of a migrating epithelial cell, which is crucial for an efficient collective migration. Yet, factors influencing the Golgi polarity remain elusive. Here we constructed a graph neural network-based deep learning model to systematically analyze the dependency of Golgi polarity on multiple g...
Source: Cells Tissues Organs - December 1, 2022 Category: Cytology Source Type: research
Protocol-dependent morphological changes in human embryonic stem cell aggregates during differentiation towards early pancreatic fate
Cell therapy is one of the promising approaches used against type1 diabetes. Efficient generation of Human embryonic stem cell (hESC)-derived pancreatic progenitors (PPs) is of great importance. Since signaling pathways underlying human pancreas development is not yet fully understood, various differentiation protocols are conducted each considering variable duration, timing and concentrations of growth factors and small molecules. Therefore, we compared two PP differentiation protocols in static suspension culture. We tested modified protocols developed by Pagliuca et al. (protocol-1) and Royan researchers (protocol-2) un...
Source: Cells Tissues Organs - November 14, 2022 Category: Cytology Source Type: research
On the variability in cell and nucleus shape
Cell morphology is an important regulator of cell function. Many abnormalities in cellular behavior can be discerned from changes in the shape of the cell and its organelles, typically the nucleus. Two major challenges for developing such phenotypic assays are reconstructing 3D surfaces of individual cells and nuclei from confocal images and developing characterizations of these surfaces for comparisons. We demonstrate two algorithms - 3D Active Contours and 3D Condensed-Attention UNet to segment cells and nuclei from confocal images. The cell and nuclear surfaces are then converted into vectors using a reversible, spheric...
Source: Cells Tissues Organs - October 31, 2022 Category: Cytology Source Type: research
MicroRNA-133a-3p inhibits lung adenocarcinoma development and cisplatin resistance through targeting GINS4
GINS subunit complex 4 (GINS4) is fundamental to DNA replication and G1/S phase transition of the cell cycle in eukaryotes. Further, recent studies implied that GINS4 can mediate the progression of several tumors, but its mechanism in lung adenocarcinoma (LUAD) is not clarified. Therefore, the role of GINS4 in LUAD was explored. MiR-133a-3p and GINS4 mRNA expression were tested through qRT-PCR. Protein levels of the two genes were assayed by western blot. Their targeting relationship was predicted and verified by bioinformatics prediction and dual-luciferase analysis. The functions of miR-133a-3p and GINS4 in LUAD were eva...
Source: Cells Tissues Organs - October 21, 2022 Category: Cytology Source Type: research
Assessing combinatorial diversity of aureochrome bZIPs through genome-wide screening
Aureochromes are unique blue light-responsive LOV (Light Oxygen Voltage) photoreceptors cum basic leucine zipper (bZIP) transcription factors (TFs), present exclusively in photosynthetic marine stramenopiles. Considering the availability of the complete genome sequence, this study focuses on aureochromes from Ectocarpus siliculosus. Aureochromes mediate light-regulated developmental responses in this brown photosynthetic algae. Both the LOV sensor and the bZIP effector shows overall sequence-structure conservation. The structurally similar LOV+bZIP modules of aureochrome homologs/paralogs prefer a dimeric state. Besides a ...
Source: Cells Tissues Organs - October 19, 2022 Category: Cytology Source Type: research
Umbilical cord blood cell clearance post-infusion in immune competent children with cerebral palsy
This study aimed to determine how quickly donor cord blood cells were cleared from the circulation in children with cerebral palsy who received a single intravenous infusion of 12/12 human leucocyte antigen (HLA) matched sibling cord blood cells. Twelve participants with cerebral palsy aged 2-12 years received cord blood cell infusions as part of a phase I trial of umbilical blood infusion for cerebral palsy. Digital droplet PCR analysis of DNA copy number variants specific to donor and recipient was used to assess donor DNA clearance at five timepoints post infusion, a surrogate measure of cell clearance. Donor cells were...
Source: Cells Tissues Organs - October 19, 2022 Category: Cytology Source Type: research
