Synthetic Small Molecules to Induce Insulin Secretion and Pancreatic Beta Cell Specific Gene Expression

In this study, a family of new SMs that are structurally similar to glinides was synthesized through three different synthetic methods and categorized into three categories (C1-C3). Then, these novel SMs were characterized by testing their effects on cell viability, pβC s-specific gene expression, and insulin secretion of MIN6 in four different concentrations and three time points. According to our results, SMs of C1 (1j, 1k, and 1l) and two SMs of C3 (1f, 1I), at 200 μM concentration, were able to increase the expression levels of Pdx1, Neurod1, Ngn3, and Ins1 as well as the insulin secretion after 24 hours. However, C2 (1a, 1b, 1c and 1d) did not show significant bio-activity of MIN6 cells. These investigated molecules can provide a tool for exploring pseudo-islet functionality in MIN6 cells or provide a possible basis for future therapeutic interventions for diabetes.
Source: Cells Tissues Organs - Category: Cytology Source Type: research