An electrochemical protocol for CRISPR-mediated gene-editing of sheep embryonic fibroblast cells

In conclusion, we developed an electrotransfection protocol using the OptiMEM-GlutaMAX medium supplemented with 3 % DMSO for sheep EF cells. The electroporation method can be used for cell-mediated gene-editing in sheep.

Source: Cells Tissues Organs - November 25, 2021 Category: Cytology Source Type: research