Extraction, degree of polymerization determination and prebiotic effect evaluation of inulin from Jerusalem artichoke
Publication date: 5 May 2015 Source:Carbohydrate Polymers, Volume 121 Author(s): Wancong Li , Jun Zhang , Chunwei Yu , Qing Li , Fang Dong , Gang Wang , Guodong Gu , Zhanyong Guo The tubers of Jerusalem artichoke are rich of inulin, which makes the plant one of primary inulin resources in China. The aim of this study was to extract inulin from tubers and test the degree of polymerization (DP) 10 days before flowering to 80 days after flowering. The DP of inulin reaches a maximum of 19 at 50 days after flowering. The variation tendencies of inulin content and DP were almost the same, which increase rapidly at th...
Source: Carbohydrate Polymers - January 14, 2015 Category: Biomedical Science Source Type: research
Jerusalem artichoke as low‐cost fructose‐rich feedstock for fossil fuels desulphurization by a fructophilic bacterium
ConclusionsThese results highlight the efficacy of the treatment applied to JAJ in making this agromaterial a promising low‐cost renewable feedstock for improved BDS by the fructophilic strain 1B.
Significance and Impact of the StudyThis study is a fundamental step viewing BDS application at the industrial level as it accounts a cost‐effective production of the biocatalysts, one of the main drawbacks for BDS scale‐up. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - January 14, 2015 Category: Microbiology Authors: T.P. Silva, S.M. Paixão, J.C. Roseiro, L. Alves Tags: Original Article Source Type: research
The use of dry Jerusalem artichoke as a functional nutrient in developing extruded food with low glycaemic index
This study considers the use of dry Jerusalem artichoke (JA) as a functional nutrient in developing food products with enhanced nutritional characteristics and low glycaemic index (GI). Three different formulations based on buckwheat and JA were developed and processed using extrusion technology. Nutritional properties including the levels of total dietary fibre (TDF), protein, inulin, total carbohydrates and lipids were analysed. A clinical study was performed on ten healthy volunteers (aged between 21 and 56) to determine the level of GI and glycaemic load (GL). The results revealed that JA significantly (P<0.05) ...
Source: Food Chemistry - January 5, 2015 Category: Food Science Source Type: research
Fibre enrichment of wheat bread with Jerusalem artichoke inulin: effect on dough rheology and bread quality
Publication date: Available online 30 December 2014 Source:Food Structure Author(s): I.A. Rubel , E.E. Pérez , G.D. Manrique , D.B. Genovese Dietary fibre enrichment of white bread with inulin-rich carbohydrate (IRC) powder extracted from Jerusalem artichoke tubers (IRC-J) was studied. Previously, it was shown that this IRC-J powder had higher prebiotic activity score than a commercial chicory inulin (IRC-C) powder, used for comparison. For bread making, 2.5 and 5.0g of either IRC-J or IRC-C were added to 100g of wheat flour, and the effects on dough viscoelastic properties and bread quality properties were analyz...
Source: Food Structure - January 1, 2015 Category: Food Science Source Type: research
The prospects of Jerusalem artichoke in functional food ingredients and bioenergy production
Publication date: Available online 13 December 2014 Source:Biotechnology Reports Author(s): Linxi Yang , Quan Sophia He , Kenneth Corscadden , Chibuike C. Udenigwe Jerusalem artichoke, a native plant to North America has recently been recognized as a promising biomass for bioeconomy development, with a number of advantages over conventional crops such as low input cultivation, high crop yield, wide adaptation to climatic and soil conditions and strong resistance to pests and plant diseases. A variety of bioproducts can be derived from Jerusalem artichoke, including inulin, fructose, natural fungicides, antioxidant ...
Source: Biotechnology Reports - December 13, 2014 Category: Biotechnology Source Type: research
Jerusalem artichoke as low‐cost fructose‐rich feedstock for fossil fuels desulfurization by a fructophilic bacterium
ConclusionsThese results highlight the efficacy of the treatment applied to JAJ in making this agro‐material a promising low‐cost renewable feedstock for improved BDS by the fructophilic strain 1B.
Significance and Impact of StudyThis study is a fundamental step viewing BDS application at the industrial level since it accounts a cost‐effective production of the biocatalysts, one of the main drawbacks for BDS scale‐up.This article is protected by copyright. All rights reserved. (Source: Journal of Applied Microbiology)
Source: Journal of Applied Microbiology - December 11, 2014 Category: Microbiology Authors: T. P. Silva, S. M. Paixão, J. C. Roseiro, L. Alves Tags: Original Article Source Type: research
Determination of fructan exohydrolase activity in the crude extracts of plants
Conclusion Enzymatic method is applicable for the routine analysis and will allow performing the investigations without special equipment on the inulin degrading enzyme in biotechnologically important crops. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - November 1, 2014 Category: Biotechnology Source Type: research
Plant prebiotics and human health: Biotechnology to breed prebiotic-rich nutritious food crops
Publication date: September 2014 Source:Electronic Journal of Biotechnology, Volume 17, Issue 5 Author(s): Sangam Dwivedi , Kanwar Sahrawat , Naveen Puppala , Rodomiro Ortiz Microbiota in the gut play essential roles in human health. Prebiotics are non-digestible complex carbohydrates that are fermented in the colon, yielding energy and short chain fatty acids, and selectively promote the growth of Bifidobacteria and Lactobacillae in the gastro-intestinal tract. Fructans and inulin are the best-characterized plant prebiotics. Many vegetable, root and tuber crops as well as some fruit crops are the best-known source...
Source: Electronic Journal of Biotechnology - November 1, 2014 Category: Biotechnology Source Type: research
A new approach for achievement of inulin accumulation in suspension cultures of Jerusalem artichoke (Helianthus tuberosus) using biotic elicitors
This study clearly indicates that combining of A. niger and Methyl-Jasmonate elicitors plays a critical role on inulin process and its accumulation in Jerusalem artichoke cell cultures. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - October 12, 2014 Category: Biotechnology Source Type: research
Cold-active and NaCl-tolerant exo-inulinase from a cold-adapted Arthrobacter sp. MN8 and its potential for use in the production of fructose at low temperatures
Publication date: Available online 27 September 2014 Source:Journal of Bioscience and Bioengineering Author(s): Junpei Zhou , Qian Lu , Mozhen Peng , Rui Zhang , Minghe Mo , Xianghua Tang , Junjun Li , Bo Xu , Junmei Ding , Zunxi Huang An exo-inulinase gene was cloned from Arthrobacter sp. MN8, a cold-adapted bacterium isolated from lead–zinc-rich soil. The gene was expressed in Escherichia coli BL21(DE3). The resultant 505-residue polypeptide (InuAMN8) showed the highest identity (81.1%) with the putative levanase from Arthrobacter phenanthrenivorans Sphe3 (ADX73279) and shared 57.8% identity with the exo-...
Source: Journal of Bioscience and Bioengineering - October 12, 2014 Category: Biomedical Science Source Type: research
Production and Characterization of a Novel Yeast Extracellular Invertase Activity Towards Improved Dibenzothiophene Biodesulfurization.
Abstract
The main goal of this work was the production and characterization of a novel invertase activity from Zygosaccharomyces bailii strain Talf1 for further application to biodesulfurization (BDS) in order to expand the exploitable alternative carbon sources to renewable sucrose-rich feedstock. The maximum invertase activity (163 U ml(-1)) was achieved after 7 days of Z. bailii strain Talf1 cultivation at pH 5.5-6.0, 25 °C, and 150 rpm in Yeast Malt Broth with 25 % Jerusalem artichoke pulp as inducer substrate. The optimum pH and temperature for the crude enzyme activity were 5.5 and 50 °C, r...
Source: Applied Biochemistry and Biotechnology - August 28, 2014 Category: Biochemistry Authors: Arez BF, Alves L, Paixão SM Tags: Appl Biochem Biotechnol Source Type: research
Improvement of the Fermentative Activity of Lactic Acid Bacteria Starter Culture by the Addition of Mn(2+)
Abstract
Production of lactic acid bacteria (LAB) starter with raw material has received much scientific investigation, but little information is available on the influences of some trace elements on the growth and fermentative activity of LAB. Based on this fact, this paper aimed to investigate the effects of Mn(2+) on the performance of Lactobacillus plantarum CX-15 starter with Jerusalem artichoke (JA) as the main medium substrate. The results showed that Mn(2+) addition had a significant beneficial affect on the fermentative activity of L. plantarum CX-15 starter. In contrast, the lack of Mn(2+) would ...
Source: Applied Biochemistry and Biotechnology - August 22, 2014 Category: Biochemistry Authors: Cheng X, Dong Y, Su P, Xiao X Tags: Appl Biochem Biotechnol Source Type: research
Optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant Saccharomyces cerevisiae carrying Kluyveromyces marxianus inulinase.
Abstract
Inulin is a polyfructan that is abundant in plants such as Jerusalem artichoke, chicory and dahlia. Inulinase can easily hydrolyze inulin to fructose, which is consumed by microorganisms. Generally, Saccharomyces cerevisiae, an industrial workhorse strain for bioethanol production, is known for not having inulinase activity. The inulinase gene from Kluyveromyces marxianus (KmINU), with the ability of converting inulin to fructose, was introduced into S. cerevisiae D452-2. The inulinase gene was fused to three different types of promoter (GPD, PGK1, truncated HXT7) and secretory signal sequence (Km...
Source: Bioprocess and Biosystems Engineering - August 21, 2014 Category: Biomedical Engineering Authors: Hong SJ, Kim HJ, Kim JW, Lee DH, Seo JH Tags: Bioprocess Biosyst Eng Source Type: research
Chronic botulism in a Saxony dairy farm: sources, predisposing factors, development of the disease and treatment possibilities.
This study is of C. botulinum types C and D at a Saxony dairy farm with 159 cows and 18 heifers. The animals exhibited clinical symptoms of chronic botulism. To determine the source of the infection, feces, blood, organs, and gastrointestinal fluids of dead or euthanized cows; as well as soil, water, silage and manure were and tested for C. botulinum spores and BoNTs using ELISA. BoNT/C and C. botulinum type C were detected in 53% and 3% of tested animals, respectively while BoNT/D and C. botulinum type D were detected in 18% of the animals. C. botulinum also was detected in organs, gastrointestinal fluids, drinking water ...
Source: Anaerobe - July 2, 2014 Category: Microbiology Authors: Krüger M, Neuhaus J, Herrenthey AG, Gökce K, Schrödl W, Shehata AA Tags: Anaerobe Source Type: research
Beneficial effects of soluble dietary Jerusalem artichoke (Helianthus tuberosus) in the prevention of the onset of type 2 diabetes and non-alcoholic fatty liver disease in high-fructose diet-fed rats.
Abstract
Jerusalem artichoke (JA) has the potential to attenuate lipid disturbances and insulin resistance (IR), but the underlying mechanisms are not well understood. In the present study, we elucidated the physiological responses and mechanisms of JA intervention with a comprehensive transcriptome analysis. Wistar rats were fed a control diet, a 60 % fructose-enriched diet (FRU), or a FRU with 10 % JA (n 6-7) for 4 weeks. An oral glucose tolerance test was carried out on day 21. Liver samples were collected for biochemical and global gene expression analyses (GeneChip® Rat Genome 230 2.0 Array, Affyme...
Source: The British Journal of Nutrition - June 26, 2014 Category: Nutrition Authors: Chang WC, Jia H, Aw W, Saito K, Hasegawa S, Kato H Tags: Br J Nutr Source Type: research
