Journal of Genetic Engineering and Biotechnology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Plant regeneration, developmental pattern and genetic fidelity of somatic embryogenesis derived Musa spp.
Publication date: December 2018Source: Journal of Genetic Engineering and Biotechnology, Volume 16, Issue 2Author(s): Natarajan Nandhakumar, Krish Kumar, Duraialagaraja Sudhakar, K. SoorianathasundaramAbstractMultiplication of banana cvs. Grand Naine (Musa AAA, Cavendish-sub group) and Rasthali (Musa AAB, Silk-sub group) were attempted through somatic embryogenesis. The influence of position of male flower buds, amino acid supplements in the induction of somatic embryogenesis and field performance of embryogenic cell suspension (ECS) derived banana plants were studied. Differentiated immature male flower buds positioned at...
Source: Journal of Genetic Engineering and Biotechnology - December 24, 2018 Category: Biotechnology Source Type: research
PNME – A gene-gene parallel network module extraction method
Publication date: Available online 11 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Bikash Jaiswal, Kumar Utkarsh, D.K. BhattacharyyaAbstractIn the domain of gene-gene network analysis, construction of co-expression networks and extraction of network modules have opened up enormous possibilities for exploring the role of genes in biological processes. Through such analysis, one can extract interesting behaviour of genes and would help in the discovery of genes participating in a common biological process. However, such network analysis methods in sequential processing mode often have been ...
Source: Journal of Genetic Engineering and Biotechnology - December 12, 2018 Category: Biotechnology Source Type: research
Recent advances in stem cells therapy: A focus on cancer, Parkinson’s and Alzheimer’s
Publication date: Available online 10 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Dalia Fleifel, Mai Atef Rahmoon, Abdelrahman AlOkda, Mostafa Nasr, Menattallah Elserafy, Sherif F. El-KhamisyAbstractStem cells serve as potential therapeutics due to their high proliferative capacity, low immunogenic reactivity and their differentiating capabilities. Several pre-clinical and early-stage clinical studies are carried out to treat genetic diseases, cancers and neurodegenerative disorders with promising preliminary results. However, there are still many challenges that scientists are trying to...
Source: Journal of Genetic Engineering and Biotechnology - December 11, 2018 Category: Biotechnology Source Type: research
Plant regeneration, developmental pattern formation and genetic fidelity of somatic embryogenesis derived Musa spp.
Publication date: Available online 10 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Natarajan Nandhakumar, Krish Kumar, Duraialagaraja Sudhakar, SoorianathasundaramAbstractMultiplication of banana cvs. Grand Naine (Musa AAA, Cavendish-sub group) and Rasthali (Musa AAB, Silk-sub group) were attempted through somatic embryogenesis. The influence of position of male flower buds, amino acid supplements in the induction of somatic embryogenesis and field performance of embryogenic cell suspension (ECS) derived banana plants were studied. Differentiated immature male flower buds positioned at 6â...
Source: Journal of Genetic Engineering and Biotechnology - December 11, 2018 Category: Biotechnology Source Type: research
Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth
Publication date: Available online 8 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Sheela Chandra, Kazim Askari, Madhumita KumariAbstractThe ability to synthesize Indole-3-acetic acid (IAA) is widely associated with the plant growth promoting rhizobacteria (PGPR). The present work deals with isolation and characterization of such bacteria from the rhizosphere of medicinal plant Stevia rebaudiana and optimization of IAA production from its isolates. The optimization of IAA production was carried out at different pH and temperature with varied carbon and nitrogen sources of culture media. Ou...
Source: Journal of Genetic Engineering and Biotechnology - December 9, 2018 Category: Biotechnology Source Type: research
Transgenic approaches for genetic improvement in groundnut (Arachis hypogaea L.) against major biotic and abiotic stress factors
Publication date: Available online 7 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Saikat Gantait, Suvendu MondalAbstractCultivated groundnut (Arachis hypogaea L.) is considered as one of the primary oilseed crops and a major fodder for cattle industry in most of the developing countries, owing to its rich source of protein. It is due to its geocarpic nature of growth that the overall yield performance of groundnut is hindered by several biotic and abiotic stress factors. Multidimensional attempts were undertaken to combat these factors by developing superior groundnut varieties, modified ...
Source: Journal of Genetic Engineering and Biotechnology - December 8, 2018 Category: Biotechnology Source Type: research
Cloning, transformation and expression of cell cycle-associated protein kinase OsWee1 in indica rice (Oryza sativa L.)
Publication date: Available online 7 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Frengky H.H. Prasetyo, Bambang Sugiharto, Netty ErmawatiAbstractThe development process of seed in plants is a cycle of cells which occur gradually and regularly. One of the genes involved in controling this stage is the Wee1 gene. Wee1 encode protein kinase which plays an important role in phosphorylation, inactivation of cyclin-dependent kinase 1 (CDK1)-cyclin (CYC) and inhibiting cell division at mitotic phase. The Overexpression of Wee1 leads to delaying entry into mitotic phase, resulting in enlargement...
Source: Journal of Genetic Engineering and Biotechnology - December 8, 2018 Category: Biotechnology Source Type: research
In silico structural and functional modelling of Antifreeze protein (AFP) sequences of Ocean pout (Zoarces americanus, Bloch & Schneider 1801)
Publication date: Available online 6 December 2018Source: Journal of Genetic Engineering and BiotechnologyAuthor(s): Manojit Bhattacharya, Arpita Hota, Avijit Kar, Deep Sankar Chini, Ramesh Chandra Malick, Bidhan Chandra Patra, Basanta Kumar DasAbstractAntifreeze proteins (AFPs) are known to polypeptide components formed by certain plants, animals, fungi and bacteria which support to survive in sub-zero temperature. Current study highlighted the seven different antifreeze proteins of fish Ocean pout (Zoarces americanus), in which protein (amino acids sequence) were collected from National Centre for Biotechnology Informati...
Source: Journal of Genetic Engineering and Biotechnology - December 7, 2018 Category: Biotechnology Source Type: research
First study of sperm mediated gene transfer in Egyptian river buffalo
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Mohamed S. Hassanane, Aida I. El Makawy, Sahar M. Helalia, Ahmed S. Abdoon, Kamal M.A. Khalil, Tharwat A. Ghanem, Amany M. Tohamy, Xiao-Feng Sun, Wei ShenAbstractThe present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfec...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Genetic vs environment influences on house mouse hybrid zone in Iran
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Nima Hashemian, Hassan Rajabi-Maham, Maryam EdrisiAbstractGenetic divergence and environment influence on speciation process are the great deal studies over recent decades. One of the best ways for exploring the interaction of geography and genetics is the evaluation of hybrids in a contact zone. To understand if there is one or more hybrid zone between house mouse subspecies in Iran and what are the differences comparing these zones with European well-known hybrid zone, we performed this approach. Samples ...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Genetic diversity of indigenous chickens from selected areas in Kenya using microsatellite markers
This study found a lot of genetic variation and relatedness within and among populations. Based on the phylogenetic tree result, it is concluded that the clustering of the chicken populations in the present study is not based on geographical proximity. The microsatellite markers used in this study were suitable for the measurement of the genetic biodiversity and relationship of Kenyan chicken populations. These results can therefore serve as an initial step to plan the conservation of indigenous chickens in Kenya. (Source: Journal of Genetic Engineering and Biotechnology)
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Application of response surface methodology to optimize the extracellular fungal mediated nanosilver green synthesis
This study aims to optimize the biosynthesis of nanosilver particles mediated by Trichoderma viride ATCC36838 using response surface methodology (RSM). Silver nanoparticles (AgNPs) were biosynthesized effectively in terms of the factors impacting silver ion (Ag+) reduction to metallic nanosilver (Ag0) using culture filtrate under shaking condition. The results of statistics calculations revealed that 2 mM silver nitrate and 28% (v/v) of culture filtrate at pH 7.0 for 34 h were the optimum values for AgNPs biosynthesis. The characterization of the produced AgNPs was conducted using electron microscopy, energy disp...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Characterization, synergistic antibacterial and free radical scavenging efficacy of silver nanoparticles synthesized using Cassia roxburghii leaf extract
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Pooja Moteriya, Hemali Padalia, Sumitra ChandaAbstractIn this work, we report for the first time, synthesis of silver nanoparticles using Cassia roxburghii leaf extract and evaluate its synergistic antibacterial efficacy and antioxidant potential. The synergistic antimicrobial activity of silver nanoparticles with commercial antibiotics against Gram-positive, Gram-negative bacteria and fungi was evaluated. The antioxidant potential of synthesized silver nanoparticles was evaluated by FRAP and ABTS radical s...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Optimization of expression JTAT protein with emphasis on transformation efficiency and IPTG concentration
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Endang Tri Margawati, Asrul Muhamad Fuad, Indriawati, Muhamad Ridwan, Slamet Diah VolkandariAbstractOne of small accessory genes between pol and env is tat gene encoding TAT protein. This research was aimed to optimize the expression of Jembrana TAT (JTAT) protein with preparing Escherichia coli (E. coli) in advance using adopted methods of M1 (MgCl2 + CaCl2) and M2 (CaCl2 + Glycerol). The best transformation efficiency resulting from a better transformation method was used to subsequent...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
Comparative effect of selenium and glycine on hydrogen peroxide-induced cell death and activation of macrophage U937 cells
Publication date: December 2017Source: Journal of Genetic Engineering and Biotechnology, Volume 15, Issue 2Author(s): Solomon A Ndoni, Tebekeme OkokoAbstractThe effects of selenium and glycine (either separately or in combination) on hydrogen peroxide-induced cell death on U937 cells and activation of U937-derived macrophages were investigated. In the first instance, U937 cells were incubated with or without selenium (Se) or glycine (GLY) or both (Se + GLY) for 24 h before exposure to hydrogen peroxide. Control cells were not incubated with Se, GLY or exposed to hydrogen peroxide. Cell viability was later assesse...
Source: Journal of Genetic Engineering and Biotechnology - December 6, 2018 Category: Biotechnology Source Type: research
