Production and evaluation of egg derived hot start antibodies
ConclusionsEgg derived Hot Start polyclonal antibodies are the cheapest source of Hot start antibodies, from one immune egg we can isolate 0.36 mg IgY, this quantity is enough for producing 1800 U activity of Hot start Taq DNA Polymerase. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 20, 2020 Category: Biotechnology Source Type: research

Improved protease activity of Pixian broad bean paste with co-cultivation of Aspergillus oryzae QM-6 and Aspergillus niger QH-3
ConclusionsThe optimal conditions of koji-making in PBP were obtained using RSM. The co-cultivation of A. oryzae and A. niger increases the overall enzyme activities in the culture medium and the FAAs content which would thus have potential application in the PBP industry. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 18, 2020 Category: Biotechnology Source Type: research

Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica
ConclusionThe PA yield from Y. lipolytica can be improved by increasing its NaCl tolerance. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 17, 2020 Category: Biotechnology Source Type: research

Genome-wide development of polymorphic microsatellite markers and their application in peanut breeding program
ConclusionsThis study reported the development of genomic SSRs from assembled genomic sequences of the tetraploid Arachis Tifrunner, which will be useful for diversity analysis, genetic mapping and functional genomics studies in peanut. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 15, 2020 Category: Biotechnology Source Type: research

Diversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica
ConclusionsThis is first report on the sea anemone-derived fungi with antimicrobial and enzyme activities. Results indicated that sea anemone is a hot spot of fungal diversity as well as a rich resource of bioactive natural products. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 15, 2020 Category: Biotechnology Source Type: research

Development of a new protocol for freeze-drying preservation of Pseudoalteromonas nigrifaciens and its protective effect on other marine bacteria
ConclusionCollectively, our results supported that the protectant mixture and parameters were beneficial for lyophilization of marine bacteria. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - January 11, 2020 Category: Biotechnology Source Type: research

Antidiabetic effect of Momordica charantia saponins in rats induced by high-fat diet combined with STZ
ConclusionMCS has an antidiabetic effect, which may be related to its improving the lipid metabolism disorder, reducing oxidative stress level, and regulating the insulin signaling pathway. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 29, 2019 Category: Biotechnology Source Type: research

Development of new protocol for freeze-drying preservation of Pseudoalteromonas nigrifaciens and its protective effect on other marine bacteria
ConclusionCollectively, our results supported that the protectant mixture and parameters were beneficial for lyophilization of marine bacteria. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 26, 2019 Category: Biotechnology Source Type: research

Prognostic value of long non-coding RNA TP73-AS1 expression in different types of cancer: A systematic review and meta-analysis
Publication date: Available online 23 December 2019Source: Electronic Journal of BiotechnologyAuthor(s): Xiaoqing Wang, Kunpeng Shu, Zhifeng Wang, Degang Ding, Xing LiAbstractBackgroundTP73 antisense RNA 1 (TP73-AS1), a newly discovered long non-coding RNA (lncRNA), has been reported to be upregulated in various kinds of tumors, and shows a variable influence on living quality and prognosis of patients. Thus, we conducted a meta-analysis to evaluate the overall prognostic value of the lncRNA TP73-AS1 in cancer patients.ResultsA systematic literature retrieval was carried out using the PubMed, Cochrane Library, EMBASE, and ...
Source: Electronic Journal of Biotechnology - December 24, 2019 Category: Biotechnology Source Type: research

The luciferase reporter system of the MMP12 endogenous promoter for investigating transcriptional regulation of the human MMP12 gene
ConclusionsThis novel luciferase knock-in reporter system will be helpful for investigating the transcriptional regulation of MMP12 gene and screening the drugs targeting MMP12 gene. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 18, 2019 Category: Biotechnology Source Type: research

Structural-functional analyses of textile dye degrading azoreductase, laccase and peroxidase: A comparative in silico study
ConclusionPresent study aims to provide knowledge on industrial dye degrading enzymes, choosing the suitable enzyme for industrial set up and to help in understanding the experimental laboratory requirements as well. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 13, 2019 Category: Biotechnology Source Type: research

Anti-diabetic effect of Momordica charantia saponins in rats induced by high-fat diet combined with STZ
ConclusionMCS has an anti-diabetic effect, which may be related to its improving the lipid metabolism disorder, reducing oxidative stress level and regulating insulin signaling pathway. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 10, 2019 Category: Biotechnology Source Type: research

Dynamic changes of total acid and bacterial communities during the traditional fermentation of Hong Qu glutinous rice wine
Publication date: Available online 10 December 2019Source: Electronic Journal of BiotechnologyAuthor(s): Zhangcheng Liang, Xiaozi Lin, Zhigang He, Weixin Li, Xiangyun Ren, Xiaojie LinAbstractBackgroundHong Qu glutinous rice wine (HQGRW) is brewed under non-aseptic fermentation conditions, so it usually has a relatively high total acid content. The aim of this study was to investigate the dynamics of the bacterial communities and total acid during the fermentation of HQGRW and elucidate the correlation between total acid and bacterial communities.ResultsThe results showed that the period of rapid acid increase during fermen...
Source: Electronic Journal of Biotechnology - December 10, 2019 Category: Biotechnology Source Type: research

Composition of the bacterial community in the gastrointestinal tract of Kunming mice
ConclusionsTo our knowledge, ours is the first study to systematically describe the gastrointestinal bacterial composition of Kunming mice. Our findings provide a better understanding of the bacterial composition of Kunming mice and serves as a foundation for the study of precision medicine. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - December 7, 2019 Category: Biotechnology Source Type: research

Ellagic acid production using polyphenols from orange peel waste by submerged fermentation
ConclusionThe submerged fermentation process is an effective methodology for the biotransformation of molecules present in orange waste to obtain high value-added as ellagic acid that can be used as powerful antioxidants, antibacterial and other applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - November 30, 2019 Category: Biotechnology Source Type: research

Improvement of anther cultures conditions using the Taguchi method in three cereal crops
ConclusionThe statistic Taguchi approach could be successfully used for various factors (here components of induction media, time of incubation on induction media) at a one time, that may impact on cereals anther cultures to improve the regeneration efficiency. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - November 30, 2019 Category: Biotechnology Source Type: research

Twin-arginine signal peptide of Bacillus licheniformis GlmU efficiently mediated secretory expression of protein glutaminase
ConclusionsPGA can be produced and secreted efficiently in active form via Tat pathway of B. licheniformis, an alternative expression system for the industrial-scale production of PGA. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 27, 2019 Category: Biotechnology Source Type: research

Acetone–butanol–ethanol fermentation from sugarcane bagasse hydrolysates: Utilization of C5 and C6 sugars
ConclusionsThe results described herein open new perspectives for the production of important molecules from residual lignocellulosic biomass for the fuel and chemical industries within the context of second-generation biorefinery. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 26, 2019 Category: Biotechnology Source Type: research

Molecular phylogenetics of black cobra (Naja naja) in Pakistan
ConclusionIt was confirmed genetically that there are two cobra species i.e., black and brown cobras in Pakistan. The study will not only help in genetic conservation but also in developing the anti-venom against snake species. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 25, 2019 Category: Biotechnology Source Type: research

Enhanced production of glutaminase-free L-asparaginase by marine Bacillus velezensis and cytotoxic activity against breast cancer cell lines
ConclusionThe study provides the first potential of glutaminase free L-asparaginase production from marine bacterium, Bacillus velezensis as a prospect anti-cancer pharmaceutical agent for two different breast cancer cell lines. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 25, 2019 Category: Biotechnology Source Type: research

Acetone-butanol-ethanol fermentation from sugarcane bagasse hydrolysates: Utilization of C5 and C6 sugars
ConclusionsThe results described herein open new perspectives for the production of important molecules from residual lignocellulosic biomass for the fuel and chemical industries within the context of second-generation biorefinery. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 25, 2019 Category: Biotechnology Source Type: research

Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. Citri
ConclusionsExpression patterns of six differentially expressed genes were validated by quantitative reverse transcription polymerase chain reaction, thus demonstrating the effectiveness of this tool in global gene expression analysis in Xac. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 25, 2019 Category: Biotechnology Source Type: research

Survival of microencapsulated Lactobacillus casei (prepared by vibration technology) in fruit juice during cold storage
ConclusionLow pH affected the survival of Lactobacillus casei under refrigeration; even when Lactobacillus casei were microencapsulated, acidic conditions impacted viability. Although pH affects viability, its value is very sensitive and will depend on the fruit juice and its composition. Some fruit juices contain compounds used as substrates for Lactobacillus and other compounds with antimicrobial effects. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - October 23, 2019 Category: Biotechnology Source Type: research

Effective diffusion coefficients and bioconversion rates of inhibitory compounds in flocs of Saccharomyces cerevisiae
ConclusionsThe obtained values for the Thiéle moduli and internal effectiveness factors showed that the bioconversion rate of the inhibitory compounds is the dominant phenomenon over the mass transfer inside the flocs. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - September 6, 2019 Category: Biotechnology Source Type: research

Purification and biochemical characterization of a novel thermophilic exo-β-1,3-glucanase from the thermophile biomass-degrading fungus Thielavia terrestris Co3Bag1
ConclusionsThis report thus describes the purification and characterization of TtBgnA, a novel exo-β-1,3-glucanase of the GH-31 family from the thermophilic fungus T. terrestris Co3Bag1. Based on the biochemical properties displayed by TtBgnA, the enzyme could be considered as a candidate for potential biotechnological applications.How to cite: Rodríguez-Mendoza J, Santiago-Hernández A, Alvarez-Zúñiga MT, et al. Purification and biochemical characterization of a novel thermophilic exo-β-1,3-glucanase from the thermophile biomass-degrading fungus Thielavia terrestris Co3Bag1. Electron J...
Source: Electronic Journal of Biotechnology - August 25, 2019 Category: Biotechnology Source Type: research

Improvement of ethanol production from birch and spruce pretreated with 1-H-3-methylmorpholinium chloride
ConclusionsThis study demonstrated the ability of [HMMorph][Cl] as an inexpensive agent to pretreat softwood as well as hardwood. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Synthesis, characterization, and in vitro evaluation of gamma radiation-induced PEGylated isoniazid
ConclusionsIn summary, the research suggests that it is possible to covalently attach the PEG onto INH by the proposed method and to obtain a slow-acting isoniazid derivative with little toxicity in vitro and higher anti-mycobacterial potency than the neat drug. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Cloning and molecular characterization of a putative habanero pepper SERK1 cDNA expressed during somatic and zygotic embryogenesis
ConclusionDNA sequence alignments and gene expression patterns suggest that CchSERK1 is the C. chinense SERK1 homolog. Significant levels of CchSERK1 transcripts were found in tissues with cell differentiation activities like vascular axes and during the development of zygotic and somatic embryos. These results suggest that CchSERK1 might have regulatory functions in cell differentiation and could be used as a biotechnological tool to study the peppers recalcitrance to proliferate in vitro. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
ConclusionsThis is the first report describing the bacterial community structure of composite soil samples of e-waste contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria having potential bioremediating properties may be present in the e-waste contaminated sites and need to be explored further. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 7, 2019 Category: Biotechnology Source Type: research

Retrovectors packaged in CHO cells to generate GLP-1-fc stable expression CHO cell lines
ConclusionsSuspension CHO cells are clean, safe, and reliable cells for retrovector packaging. Retrovectors packaged from this system could be used to generated CHO stable cell lines for recombinant protein expression. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - August 3, 2019 Category: Biotechnology Source Type: research

Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high-gravity bioethanol fermentation
ConclusionsA multiple-stress-tolerant yeast clone was obtained using adaptive evolution by a freeze–thaw method coupled with stress shock selection. The selected robust yeast strain exhibits potential for bioethanol production through VHG fermentation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 20, 2019 Category: Biotechnology Source Type: research

A novel thermophilic exo-β-1,3-glucanase from the thermophile biomass-degrading fungus Thielavia terrestris Co3Bag1. Purification and biochemical characterization of the enzyme
ConclusionsThis report thus describes the purification and characterization of TtBgnA, a novel exo-β-1,3-glucanase GH-31 family from the thermophilic fungus T. terrestris Co3Bag1. Based on the biochemical properties displayed by TtBgnA, the enzyme could be considered as a candidate for potential biotechnological applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 18, 2019 Category: Biotechnology Source Type: research

Discovery and molecular analysis of conserved circRNAs from cashmere goat reveal their integrated regulatory network and potential roles in secondary hair follicle
ConclusionsA total of eighteen circular RNAs were identified and characterized from skin tissue of cashmere goat. The eight up-regulated circular RNAs at anagen might have significant roles in secondary hair follicle of cashmere goat. Our results would provide novel regulatory layer to elucidating the molecular mechanisms underlying the development of secondary hair follicle and the growth of cashmere fiber in cashmere goat. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 10, 2019 Category: Biotechnology Source Type: research

Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high gravity bioethanol fermentation
ConclusionsA multiple-stress-tolerant yeast clone was obtained using adaptive evolution via a freeze–thaw method coupled with stress shock selection. The selected robust yeast strain exhibits potential for bioethanol production via VHG fermentation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 10, 2019 Category: Biotechnology Source Type: research

An efficient method of Yarrowia lipolytica immobilization using oil- and emulsion-modified bacterial cellulose carrier
ConclusionThe application of oil- or emulsion-modified BC as carriers significantly increased efficiency of Y. lipolytica immobilization as compared to unmodified BC. The addition of oil or emulsion to the culture medium can be a simple but effective method to modify the porosity of BC-based carriers. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - July 3, 2019 Category: Biotechnology Source Type: research

Le arning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection
Publication date: Available online 27 June 2019Source: Electronic Journal of BiotechnologyAuthor(s): Ariel D. Arencibia, Vívian D'Afonseca, Mohan Chakravarthi, Stefano CastiglioneAbstractWe highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants...
Source: Electronic Journal of Biotechnology - June 28, 2019 Category: Biotechnology Source Type: research

Learning from transgenics: Advanced gene editing technologies should also bridge the gap with traditional genetic selection
Publication date: Available online 27 June 2019Source: Electronic Journal of BiotechnologyAuthor(s): Ariel D. Arencibia, Vívian D'Afonseca, Mohan Chakravarthi, Stefano CastiglioneAbstractWe highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants...
Source: Electronic Journal of Biotechnology - June 27, 2019 Category: Biotechnology Source Type: research

Co-production of ethanol and biodiesel from sweet sorghum juice in two consecutive fermentation steps
ConclusionThe two step process is viable and could be considered an integral part of a consolidated biorefinery from sweet sorghum. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - June 25, 2019 Category: Biotechnology Source Type: research

Assessment of wasteland derived biomass for bioethanol production
ConclusionsThe plants such as fireweed, common broom, hay and goldenrod may be useful for the production of liquid biofuels and it would be necessary in the further stage of research to establish and optimize the conditions for the technology of ethyl alcohol producing from these plant species. Enzymatic hydrolysis of biomass from agricultural wastelands results in a large increase in fermentable sugars, comparable to the enzymatic hydrolysis of rye, wheat, rice or maize straw. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 24, 2019 Category: Biotechnology Source Type: research

Enzymatic preparation of fructooligosaccharides-rich burdock syrup with enhanced antioxidative properties
ConclusionsOur results could be applied to the development of efficient extraction of valuable products from agricultural materials using enzyme-mediated methods. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 15, 2019 Category: Biotechnology Source Type: research

Productive infection and transduction by bacteriophage P1 in the species Salmonella bongori
ConclusionTherefore, bacteriophage P1 can be used as a tool for the genetic manipulation in the species S. bongori. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 12, 2019 Category: Biotechnology Source Type: research

Expression and purification of the transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli
ConclusionsA new gene, named StMSN2, with 1617 bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody, named Msn2-2, against StMsn2 with high specificity was identified. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 9, 2019 Category: Biotechnology Source Type: research

Modeling of lactose enzymatic hydrolysis using Monte Carlo method
ConclusionsMonte Carlo modeling method can be used to model enzymatic reactions in a simple way for real applications. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - May 4, 2019 Category: Biotechnology Source Type: research

Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets
ConclusionsThis is a promising strain for producing prodigiosin. The prodigiosin hase potential in anticancer medicine exploration. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 27, 2019 Category: Biotechnology Source Type: research

A comparison of callus induction in 4 Garcinia species
ConclusionsFor all 4 species, the greatest amount of large nodular callus was observed in the TDZ treatments. White, friable callus was observed on most of the 2,4-D and picloram treatment groups. Most meta-topolin treatments resulted in minimal callus formation. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 27, 2019 Category: Biotechnology Source Type: research

Expression and purification of a transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli
ConclusionsA new gene name StMSN2 with 1617 bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody named Msn2–2 against StMsn2 with high specificity was identified. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 26, 2019 Category: Biotechnology Source Type: research

Development of biodegradation process for poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16–1
ConclusionsThis is the first report demonstrating the scaling up of PLA degradation in a 5 L bioreactor and the evaluation of a potential method for enhancing PLA degradation efficiency. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 24, 2019 Category: Biotechnology Source Type: research

Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115
ConclusionsFormaldehyde is produced inside a biofilter when methanol is treated in a biofiltration system inoculated with Pichia pastoris. Biomass present in the reactor is capable of degrading the formaldehyde generated as the concentration of methanol decreases. However, high methanol loads can lead to the generation and release of formaldehyde into the environment. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Polymorphism of bovine lipocalin-2 gene and its impact on milk production traits and mastitis in Holstein Friesian cattle
ConclusionsAlthough, the identified SNPs were not found to have any impact on the somatic cell count or the incidence of mastitis in cows, it seems that further research is necessary, covering a larger population of cattle, to confirm the association between lipocalin-2 and milk production traits and mastitis. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Chlorella sorokiniana and Chlorella minutissima exhibit antioxidant potentials, inhibit cholinesterases and modulate disaggregation of β-amyloid fibrils
This study reveals the modulatory effects of C. sorokiniana and C. minutissima extracts on some mediators of Alzheimer's disease and provides insights into their potential benefits as functional food, nutraceutics or therapeutic agent for the management of this neurodegenerative disease. (Source: Electronic Journal of Biotechnology)
Source: Electronic Journal of Biotechnology - April 12, 2019 Category: Biotechnology Source Type: research