Gene Cloning, Expression, and Characterization of an Exo-inulinase from Paenibacillus polymyxa ZJ-9.
Abstract
An inulinase-producing strain, Paenibacillus polymyxa ZJ-9, was isolated from natural sources to produce R,R-2,3-butanediol via one-step fermentation of raw inulin extracted from Jerusalem artichoke tubers. The inulinase gene from P. polymyxa ZJ-9 was cloned and overexpressed in Escherichia coli BL21 (DE3), and the purified recombinant inulinase was estimated to be approximately 56 kDa by both sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography. This result suggests that the active form of the inulinase is probably a monomer. Terminal hydrolysis ...
Source: Applied Biochemistry and Biotechnology - May 8, 2014 Category: Biochemistry Authors: Gao J, Xu YY, Yang HM, Xu H, Xue F, Li S, Feng XH Tags: Appl Biochem Biotechnol Source Type: research
Functional analyses of a putative plasma membrane Na(+)/H (+) antiporter gene isolated from salt tolerant Helianthus tuberosus.
Abstract
Jerusalem artichokes (Helianthus tuberosus L.) can tolerate relatively higher salinity, drought and heat stress. In this paper, we report the cloning of a Salt Overly Sensitive 1 (SOS1) gene encoding a plasma membrane Na(+)/H(+) antiporter from a highly salt-tolerant genotype of H. tuberosus, NY1, named HtSOS1 and characterization of its function in yeast and rice. The amino acid sequence of HtSOS1 showed 83.4 % identity with the previously isolated SOS1 gene from the Chrysanthemum crassum. The mRNA level in the leaves of H. tuberosus was significantly up-regulated by presence of high concentrati...
Source: Molecular Biology Reports - April 26, 2014 Category: Molecular Biology Authors: Li Q, Tang Z, Hu Y, Yu L, Liu Z, Xu G Tags: Mol Biol Rep Source Type: research
Chemical Composition of the Tuber Essential Oil from Helianthus tuberosus L. (Asteraceae).
Abstract
Helianthus tuberosus L. (Jerusalem artichoke) is cultivated in Europe and other parts of the world as a food crop and ornamental plant. The volatile oils of the aerial parts of H. tuberosus were investigated more than 30 years ago, but no study could be found to date on the constituents of the tuber essential oil. Herein, the first characterization by GC-FID, GC/MS, and (13) C-NMR analyses of a hydrodistilled essential oil of Jerusalem artichoke tubers was reported. Fresh plant material collected in Serbia (Sample A) and a commercial sample (Sample B) yielded only small amounts of oil (0.0014 and ...
Source: Chemistry and Biodiversity - March 1, 2014 Category: Biochemistry Authors: Radulović NS, Ethorđević MR Tags: Chem Biodivers Source Type: research
Effects of feeding different roughage components to sows in gestation on bacteriological and immunological parameters in colostrum and immune response of piglets.
Abstract
In piglet production, Escherichia coli (E. coli) and the presence of lipopolysaccharides (LPS) were considered as predominant causative agents in the aetiology of important diseases of sows and piglets. The aim of the study was to assess the effects of feeding different roughage sources to sows in gestation on the microbiota and endotoxin concentration in colostrum and on the specific immune response of their piglets. In two trial runs, a total of 144 sows were assigned to one of the six dietary treatments: the Control group receiving a concentrate diet and five groups with a restrictive supply of...
Source: Archives of Animal Nutrition - January 21, 2014 Category: Nutrition Authors: Werner C, Schubbert A, Schrödl W, Krüger M, Sundrum A Tags: Arch Anim Nutr Source Type: research
Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp.
Abstract
After the endo-inulinase gene from Arthrobacter sp. was ligated the expression vectors pMIDSC31 and pMIRSC31, the endo-inulinase gene was inserted into the chromosomal DNA of Saccharomyces sp. W0. It was found that the inulinase activity of the recombinant yeast D5 in which the endo-inulinase gene was inserted into the delta sequence was higher than that of the recombinant yeast R1 in which the endo-inulinase gene was inserted into 18S rDNA sequence. More ethanol from inulin was produced by the recombinant yeast D5 than by the recombinant yeast R1. But Saccharomyces sp. W0 produced the lowest inul...
Source: Bioresource Technology - August 15, 2013 Category: Biotechnology Authors: Li Y, Liu GL, Chi ZM Tags: Bioresour Technol Source Type: research
Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.
Abstract
To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kut...
Source: Biotechnology Letters - June 7, 2013 Category: Biotechnology Authors: Yuan B, Wang SA, Li FL Tags: Biotechnol Lett Source Type: research
Penicillium subrubescens, a new species efficiently producing inulinase.
Abstract
Inulin is a reserve carbohydrate in about 15 % of the flowering plants and is accumulated in underground tubers of e.g. chicory, dahlia and Jerusalem artichoke. This carbohydrate consists of linear chains of β-(2,1)-linked fructose attached to a sucrose molecule. Inulinases hydrolyse inulin into fructose and glucose. To find efficient inulin degrading fungi, 126 fungal strains from the Fungal Biotechnology Culture Collection (FBCC) at University of Helsinki and 74 freshly isolated strains from soil around Jerusalem artichoke tubers were screened in liquid cultures with inulin as a sole source of...
Source: Antonie van Leeuwenhoek - May 23, 2013 Category: Microbiology Authors: Mansouri S, Houbraken J, Samson RA, Frisvad JC, Christensen M, Tuthill DE, Koutaniemi S, Hatakka A, Lankinen P Tags: Antonie Van Leeuwenhoek Source Type: research
Citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 and purification of citric acid.
In this study, citric acid production from extract of Jerusalem artichoke tubers by the genetically engineered yeast Yarrowia lipolytica strain 30 was investigated. After the compositions of the extract of Jerusalem artichoke tubers for citric acid production were optimized, the results showed that natural components of extract of Jerusalem artichoke tubers without addition of any other components were suitable for citric acid production by the yeast strain. During 10 L fermentation using the extract containing 84.3 g L(-1) total sugars, 68.3 g L(-1) citric acid was produced and the yield of citric acid was 0.91 g g...
Source: Bioprocess and Biosystems Engineering - April 14, 2013 Category: Biomedical Engineering Authors: Wang LF, Wang ZP, Liu XY, Chi ZM Tags: Bioprocess Biosyst Eng Source Type: research
Consolidated bioprocessing of highly concentrated Jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation
In this study, Saccharomyces cerevisiae DQ1 was found to produce relatively large amount of inulinase for hydrolysis of inulin in Jat, and the helical ribbon stirring bioreactor used provided well mixing performance under the high Jat loading. Even a highly concentrated Jat loading up to 35% (w/w) in the helical ribbon bioreactor for CBP was allowed. The results obtained from this study have demonstrated a feasibility of developing a CBP process technology in the helical ribbon bioreactor for ethanol production at a high yield 128.7 g/L and the theoretical yield 73.5%, respectively. This level of ethanol yield from Jat is ...
Source: Biotechnology and Bioengineering - April 8, 2013 Category: Biomedical Science Authors: Lihao Guo, Jian Zhang, Fengxian Hu, Dewey DY Ryu, Jie Bao Tags: Article Source Type: research
Screening of novel yeast inulinases and further application to bioprocesses.
In this study, several microbial strains were isolated and their ability to inulinase biosynthesis was evaluated. The novel yeast strain Talf1, identified as Zygosaccharomyces bailii, was the best inulinase producer, attaining 8.67 U/ml of inulinase activity when JA juice was used as the inducer substrate. Z. bailii strain Talf1 and/or its enzymatic crude extract were further applied for bioethanol production and biodesulfurization (BDS) processes, using inulin and JA juice as carbon source. In a consolidated bioprocessing for ethanol production from 200g/l inulin, Z. bailii strain Talf1 was able to produce 67g/l of ethano...
Source: New Biotechnology - February 15, 2013 Category: Biotechnology Authors: Paixão SM, Teixeira PD, Silva TP, Teixeira AV, Alves L Tags: N Biotechnol Source Type: research
Ethanol Production Using Whole Plant Biomass of Jerusalem Artichoke by Kluyveromyces marxianus CBS1555.
In this study, Jerusalem artichoke stalk was pretreated sequentially with dilute acid and alkali, and then hydrolyzed enzymatically. During enzymatic hydrolysis, approximately 88 % of the glucan and xylan were converted to glucose and xylose, respectively. Batch and fed-batch simultaneous saccharification and fermentation of both pretreated stalk and tuber by Kluyveromyces marxianus CBS1555 were effectively performed, yielding 29.1 and 70.2 g/L ethanol, respectively. In fed-batch fermentation, ethanol productivity was 0.255 g ethanol per gram of dry Jerusalem artichoke biomass, or 0.361 g ethanol per gram of glucose, w...
Source: Applied Biochemistry and Biotechnology - January 16, 2013 Category: Biochemistry Authors: Kim S, Park JM, Kim CH Tags: Appl Biochem Biotechnol Source Type: research
