Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.

Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.
Biotechnol Lett. 2013 Jun 7;
Authors: Yuan B, Wang SA, Li FL
Abstract
To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources.
PMID: 23743955 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - June 7, 2013 Category: Biotechnology Authors: Yuan B, Wang SA, Li FL Tags: Biotechnol Lett Source Type: research