Jerusalem artichoke diversity in T ürkiye: comparative analysis of clone and organ-based biochemical composition
In this study, biochemical analyses were performed for the first time on 22 different Jerusalem artichokes clones collected from different regions of Türkiye and samples from three different organs of each clone, considering the interaction effects. As a result of the study, the interaction effect of clones and sampled organs was found significant for total flavonoids, ascorbic acid, total chlorophyll, pH, H2O2 removal capacity, total dry matter, water soluble dry matter and antioxidant capacity via FRAP. According to the correlation analysis, the highest coefficient among the parameters in the tuber was between titratabl...
Source: Natural Product Research - November 15, 2023 Category: Biochemistry Authors: Fatih Hanci Zelal Inan Akife Dalda Şekerci Ayd ın Uzun Source Type: research
Jerusalem artichoke diversity in T ürkiye: comparative analysis of clone and organ-based biochemical composition
In this study, biochemical analyses were performed for the first time on 22 different Jerusalem artichokes clones collected from different regions of Türkiye and samples from three different organs of each clone, considering the interaction effects. As a result of the study, the interaction effect of clones and sampled organs was found significant for total flavonoids, ascorbic acid, total chlorophyll, pH, H2O2 removal capacity, total dry matter, water soluble dry matter and antioxidant capacity via FRAP. According to the correlation analysis, the highest coefficient among the parameters in the tuber was between titratabl...
Source: Natural Product Research - November 15, 2023 Category: Biochemistry Authors: Fatih Hanci Zelal Inan Akife Dalda Şekerci Ayd ın Uzun Source Type: research
Identification and expression analysis of miR396 and its target genes in Jerusalem artichoke under temperature stress
In this study, we successfully cloned and identified three MIR396s (htu-MIR396a/b/c) and their corresponding miR396s (htu-miR396a/b/c). Additionally, we identified nine target genes, comprising seven GRFs (HtGRF3/4/6/9/10/12/13), one WRKY (HtWRKY40), and one SCL (HtSCL33), using comprehensive sRNAome, transcriptome, and degradome data of Jerusalem artichoke. The expression patterns of htu-MIR396s, htu-miR396s, and their targets in tissues and under temperature stress were analyzed by qRT-PCR and RNA-seq. Notably, htu-MIR396s exhibited high expression in seedling stems, while htu-miR396s predominantly expressed in seedling ...
Source: Gene - October 20, 2023 Category: Genetics & Stem Cells Authors: Baishui Ding Yang Yue Xi Chen Xiaohua Long Zhaosheng Zhou Source Type: research
Identification and expression analysis of miR396 and its target genes in Jerusalem artichoke under temperature stress
In this study, we successfully cloned and identified three MIR396s (htu-MIR396a/b/c) and their corresponding miR396s (htu-miR396a/b/c). Additionally, we identified nine target genes, comprising seven GRFs (HtGRF3/4/6/9/10/12/13), one WRKY (HtWRKY40), and one SCL (HtSCL33), using comprehensive sRNAome, transcriptome, and degradome data of Jerusalem artichoke. The expression patterns of htu-MIR396s, htu-miR396s, and their targets in tissues and under temperature stress were analyzed by qRT-PCR and RNA-seq. Notably, htu-MIR396s exhibited high expression in seedling stems, while htu-miR396s predominantly expressed in seedling ...
Source: Gene - October 20, 2023 Category: Genetics & Stem Cells Authors: Baishui Ding Yang Yue Xi Chen Xiaohua Long Zhaosheng Zhou Source Type: research
Unveiling HSP40/60/70/90/100 gene families and abiotic stress responses in Jerusalem artichoke
Gene. 2023 Oct 18:147912. doi: 10.1016/j.gene.2023.147912. Online ahead of print.ABSTRACTHeat shock proteins (HSPs) are essential for plant growth, development, and stress adaptation. However, their roles in Jerusalem artichoke are largely unexplored. Using bioinformatics, we classified 143 HSP genes into distinct families: HSP40 (82 genes), HSP60 (22 genes), HSP70 (29 genes), HSP90 (6 genes), and HSP100 (4 genes). Our analysis covered their traits, evolution, and structures. Using RNA-seq data, we uncovered unique expression patterns of these HSP genes across growth stages and tissues. Notably, HSP40, HSP60, HSP70, HSP90,...
Source: Gene - October 20, 2023 Category: Genetics & Stem Cells Authors: Wencai Ren Baishui Ding Wenhan Dong Yang Yue Xiaohua Long Zhaosheng Zhou Source Type: research
Sonochemical application reduces monosaccharide levels and improves cryoprotective effect of Jerusalem artichoke extract on Leuconostoc mesenteroides WiKim33 during freeze-drying
This study suggests a novel cryoprotective agent derived from Jerusalem artichoke (JA; Helianthus tuberous L.) and describes the mechanism of cryoprotective effect improvement by sonication treatment. The cryoprotective effect of JA extract was verified by examining the viability of Leuconostoc mesenteroides WiKim33 after freeze-drying (FD). Sonication of JA extract improved the cryoprotective effect. Sonication reduced fructose and glucose contents, which increased the induction of critical damage during FD by 15.84% and 46.81%, respectively. The cryoprotective effects of JA and sonication-treated JA extracts were determi...
Source: Ultrasonics Sonochemistry - April 23, 2023 Category: Chemistry Authors: Yeong Yeol Kim Ho Myeong Kim Seul-Gi Jeong Jung Eun Yang Seulbi Kim Hae Woong Park Source Type: research
