Identification and expression analysis of miR396 and its target genes in Jerusalem artichoke under temperature stress

In this study, we successfully cloned and identified three MIR396s (htu-MIR396a/b/c) and their corresponding miR396s (htu-miR396a/b/c). Additionally, we identified nine target genes, comprising seven GRFs (HtGRF3/4/6/9/10/12/13), one WRKY (HtWRKY40), and one SCL (HtSCL33), using comprehensive sRNAome, transcriptome, and degradome data of Jerusalem artichoke. The expression patterns of htu-MIR396s, htu-miR396s, and their targets in tissues and under temperature stress were analyzed by qRT-PCR and RNA-seq. Notably, htu-MIR396s exhibited high expression in seedling stems, while htu-miR396s predominantly expressed in seedling leaves. Moreover, HtWRKY40 and HtSCL33 displayed higher expression levels than HtGRFs in most tissues, except leaves. Remarkably, HtGRF4/6/10/12/13 exhibited higher expression in leaves than in roots and stems during seedling growth. Furthermore, during tuber development, HtGRF4/6/10, HtWRKY40, and HtSCL33 were highly expressed, while HtGRF3/9/12/13 showed relatively lower expression levels. Under heat stress (42℃), htu-MIR396 expression was up-regulated, and htu-miR396 showed dynamic expression patterns in seedlings, resulting in the induction of HtGRF4/6/10/12/13 in leaves and HtSCL33 in roots, while HtWRKY40 in leaves was repressed. Conversely, under cold stress (4℃), htu-MIR396s showed fluctuating expression levels, and htu-miR396s were up-regulated in seedlings. Notably, HtGRF4/13 and HtSCL33 in seedlings were reduced, whereas HtGRF6 in roots and Ht...
Source: Gene - Category: Genetics & Stem Cells Authors: Source Type: research