Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher
Publication date: Available online 11 January 2020Source: Biotechnology ReportsAuthor(s): Hiroki Akiba, Kensuke Takayanagi, Osamu Kusano-Arai, Hiroko Iwanari, Takao Hamakubo, Kouhei TsumotoAbstractBiparatopic fragment antibodies can overcome deficiencies in avidity of conventional antibody fragments. Here, we describe a technology for generating biparatopic antibodies through two-step targeting using a pair of polypeptides, SpyTag and SpyCatcher, that spontaneously react to form a covalent bond between antibody fragments. In this method, two antibody fragments, each targeting different epitopes of the antigen, are fused to...
Source: Biotechnology Reports - January 12, 2020 Category: Biotechnology Source Type: research

The complete genome sequence of the algicidal bacterium Bacillus subtilis strain JA and the use of quorum sensing to evaluate its antialgal ability
We describe the isolation of Bacillus subtilis strain JA and demonstrate that this bacterium exhibited strong algicidal effects on the algae Alexandrium minutum with an inhibition rate exceeding 80% within 48 h. B. subtilis JA significantly reduced the photosynthetic efficiency of A. minutum and caused extensive morphological damage to the algae. Genomic analysis of B. subtilis JA demonstrated that a putative AI-2 type quorum sensing (QS) gene (LuxS) is present in its genome cluster, which is regulate pheromone biosynthesis. Interestingly, the exogenous addition of a QS-oligopeptide (ComX-pheromone) improved the algicidal ...
Source: Biotechnology Reports - January 11, 2020 Category: Biotechnology Source Type: research

Phosphorus Removal from Landfill Leachate by Microalgae
Publication date: Available online 7 January 2020Source: Biotechnology ReportsAuthor(s): Zareen T. KhanzadaAbstractPhosphorus is an essential constituent of all living organisms but it is non-renewable and its natural reserves are fast depleting. Phosphorus discharged in wastewater could be sustainably reused by microalgae. Knowledge about cellular phosphorus dynamics in microalgae has been rapidly advancing and luxury phosphorus (poly-P) uptake phenomenon by microalgae is becoming the focus point for many research studies. Ultra-membrane treated landfill leachate was used as a nutrient medium for the growth of indigenous ...
Source: Biotechnology Reports - January 8, 2020 Category: Biotechnology Source Type: research

Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers in Morus alba L. cv. Chinese white
Publication date: Available online 3 January 2020Source: Biotechnology ReportsAuthor(s): Gulab Khan Rohela, Phanikanth Jogam, Mohammad Yaseen Mir, Aftab Ahmad Shabnam, Pawan Shukla, Sadanandam Abbagani, Azra Nahaid KamiliAbstractA reliable protocol was developed for in vitro micro propagation of Morus alba L.cv. Chinese white. Initially, friable callus was induced (242.8 and 128.5 mg) from in vivo leaf and nodal explants on Murashige and Skoog’s (MS) medium amended with 4.0 µM/L of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 3.0 µM/L of Naphthalene acetic acid (NAA) respectively within 3 weeks. Shoo...
Source: Biotechnology Reports - January 5, 2020 Category: Biotechnology Source Type: research

Impact of process parameters and plant polysaccharide hydrolysates in cellulase production by Trichoderma reesei and Neurospora crassa under wheat bran based solid state fermentation
Publication date: Available online 2 January 2020Source: Biotechnology ReportsAuthor(s): Nitin Verma, Vivek KumarAbstractLignocellulosic biomass is a promising feedstock for future renewable fuels. It constitutes a substantial renewable substrate for the production of value-added chemicals. Lignocellulosic materials mostly contain a mixture of polymers such as cellulose, hemicelluloses, and lignin. Utilizing waste lignocellulosic materials such as agricultural residues, grasses, forestry wastes can significantly reduce the cost of raw materials. Optimization of significant process parameters is also a very important stage ...
Source: Biotechnology Reports - January 3, 2020 Category: Biotechnology Source Type: research

Draft genome sequence and nomenclature adjustment of Rhodococcus qingshengii CS98, a cesium-accumulating strain isolated in Japan
Publication date: Available online 31 December 2019Source: Biotechnology ReportsAuthor(s): Candice Raeburn, Shin Kasahara, Toshi Komoda, Catherine Abbott, Peter M SmookerAbstractStrains within the Rhodococcus genus have the ability to endure a range of recalcitrant compounds and metabolise a variety of pollutants. As a result there is increasing interest in these robust prokaryotes for their applications in bioremediation of contaminated environments and bioconversion of industrial wastes. In this announcement we present the draft genome sequence of R. qingshengii CS98, a soil isolate from Japan with the demonstrated abili...
Source: Biotechnology Reports - January 2, 2020 Category: Biotechnology Source Type: research

Investigation of antifouling properties of polypropylene/TiO2 nanocomposite membrane under different aeration rate in membrane bioreactor system
Publication date: Available online 31 December 2019Source: Biotechnology ReportsAuthor(s): Habib Etemadi, Milad Fonouni, Reza YeganiAbstractThis paper presents the results of aeration rate effect on antifouling properties of polypropylene (PP)/TiO2 nanocomposite membrane in membrane bioreactor (MBR) system in order to oil refinery wastewater treatment. For this purpose, three levels of aeration rate with specific aeration demand per membrane area (SADm) of 0.5, 1, and 1.5 m3/m2h was used. According to the obtained results, PP nanocomposite membrane showed high hydrophilicity, porosity, and high flux compared to neat...
Source: Biotechnology Reports - January 2, 2020 Category: Biotechnology Source Type: research

Relevance of biogas technology to vhembe district of the limpopo province in south africa
In this study, the sampled households involved 72 biogas users and 128 non-users. The sampling techniques was purposive and simple random. The study was based on primary data that was elicited using open and closed-ended questionnaires. The logistic regression model was employed for data analysis. The energy demand of low-income South African households for cooking with fuelwood was found to be 27 MJ/day and the total energy demand 68 MJ/day. Approximately 625 000 households in Vhembe district can potentially benefit from bio-digester fed with cattle and pig waste, on the basis of livestock numbers. Most gove...
Source: Biotechnology Reports - December 21, 2019 Category: Biotechnology Source Type: research

Draft genomes and initial characterization of siderophore producing pseudomonads isolated from mine dump and mine drainage
Publication date: March 2020Source: Biotechnology Reports, Volume 25Author(s): Marika Hofmann, Thomas Heine, Vivian Schulz, Sarah Hofmann, Dirk TischlerAbstractSiderophores are of high interest for biotechnological, pharmaceutical, agricultural and industrial applications. Although they are synthesized by various organisms, the yield is usually low which hindrances their suitability for broad range uses. Thus, it is necessary to identify novel producers and to increase the understanding of the biosynthesis pathways. Herein we report the isolation of two novel Pseudomonas strains and the identification of the gene clusters ...
Source: Biotechnology Reports - December 18, 2019 Category: Biotechnology Source Type: research

Isolation and characterization of an alkali and thermostable laccase from a novel Alcaligenes faecalis and its application in decolorization of synthetic dyes
Publication date: Available online 14 December 2019Source: Biotechnology ReportsAuthor(s): Seema Mehandia, S.C. Sharma, Shailendra Kumar AryaAbstractA laccase producing new bacterial strain (Alcaligenes faecalis XF1) was isolated from green site of Chandigarh (India) by standard screening method. Nutrient broth medium containing 0.2 mM CuSO4 was used for the production of laccase. Maximum production (110 U/ml) was achieved after four days of incubation. The extracellular laccase from the medium was purified by simple salt precipitation and ion exchange technique to get 3.8 fold purified protein with 1637.8 U/mg spec...
Source: Biotechnology Reports - December 15, 2019 Category: Biotechnology Source Type: research

Sequential degradation of raw vinasse by a laccase enzyme producing fungus Pleurotus sajor-caju and its ATPS purification
This study evaluated simultaneously the raw vinasse degradation, an effluent from the sugar-alcohol industry, the laccase production by Pleurotus sajor-caju and its purification using aqueous two-phase systems (ATPS). To improve laccase production, different concentrations of inducers (ethanol and CuSO4) were added. The higher laccase production promoted an increase of 4-fold using 0.4 mM of CuSO4 as inducer, with maximum enzymatic activity of 539.3 U/L on the 3rd day of fermentation. The final treated vinasse had a decolorization of 92% and turbidity removal of 99% using CuSO4. Moreover, the produced laccase was th...
Source: Biotechnology Reports - December 13, 2019 Category: Biotechnology Source Type: research

Removal of Pb (II) from aqueous solution using nanoadsorbent of Oryza sativa husk: Isotherm, Kinetic and Thermodynamic studies
Publication date: Available online 6 December 2019Source: Biotechnology ReportsAuthor(s): Mandeep Kaur, Santosh Kumari, Praveen SharmaAbstractThis research focus on the removal of Pb (II) ions from aqueous solution by adsorption process using nanoadsorbent developed from agricultural waste Oryza sativa husk (OSH). Surface morphology of nanoadsorbent was analyzed by FE-SEM, elemental composition by EDX and size by AFM. Attachment of functional groups on nanoadsorbent was determined by FTIR. The effect of pH, dose, contact time, initial concentration and temperature were investigated. Optimum adsorption of lead at pH 8, cont...
Source: Biotechnology Reports - December 7, 2019 Category: Biotechnology Source Type: research

Plant growth-promoting activities and genomic analysis of the stress-resistant Bacillus megaterium STB1, a bacterium of agricultural and biotechnological interest
Publication date: Available online 4 December 2019Source: Biotechnology ReportsAuthor(s): Francisco X. Nascimento, Anabel G. Hernández, Bernard R. Glick, Márcio J. RossiAbstractIn this work, the stress-resistant Bacillus megaterium STB1 is characterized and its ability to promote plant growth under normal and stress conditions is demonstrated. The genomic sequence of this bacterium, and a detailed analysis of the genes involved in facilitating its stress resistance and plant growth-promoting activities is also reported.The B. megaterium STB1 genome is rich in genetic elements involved in multiple stress resis...
Source: Biotechnology Reports - December 5, 2019 Category: Biotechnology Source Type: research

Optimization of Carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in Bioreactor: A statistical approach
Publication date: Available online 5 December 2019Source: Biotechnology ReportsAuthor(s): Rajan Sharma, Gargi GhoshalAbstractBio-colorants are preferred over synthetic colors as bio-colorants not only impart characteristic color to the food also contain harmless bio-active antioxidant nutrients. The present study was undertaken to investigate the potential of agro-industrial waste (Onion peels, potato skin, mung bean husk and pea pods) for carotenoid production from Rhodotorula mucilaginosa. After screening of appropriate carbon, nitrogen sources from agro-industrial waste, the fermentation conditions (pH, temperature, agi...
Source: Biotechnology Reports - December 5, 2019 Category: Biotechnology Source Type: research

Study of antioxidant activity and some herbal compounds of Dracocephalum kotschyi Boiss. in different ages of growth
ConclusionBut in general, it can be said that the six-year-old plant with the highest antioxidant capacity, phenolic, and flavonoid compounds is the best age and mainly flower organ is preferred. (Source: Biotechnology Reports)
Source: Biotechnology Reports - December 4, 2019 Category: Biotechnology Source Type: research

Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: from dye decolorization to denim bioscouring
Publication date: Available online 3 December 2019Source: Biotechnology ReportsAuthor(s): John O. UnuofinAbstractThe aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccase which were secreted in media containing environmental wastes were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. There...
Source: Biotechnology Reports - December 4, 2019 Category: Biotechnology Source Type: research

Controlling Anthracnose By Means of extracts, and their major constituents, from Brosimum rubescens Taub
Publication date: Available online 30 November 2019Source: Biotechnology ReportsAuthor(s): Janio Martinez, Andrés Gomez, Cesar Ramirez, Jesús Gil, Diego DurangoAbstractAnthracnose, caused by the fungus Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., is the most limiting fungal disease of mango and papaya crops in Colombia. The in vivo and in vitro activity against C. gloeosporioides of the extracts from sawdust of Brosimum rubescens Taub. (Moraceae) was evaluated. The extracts of less polarity (n-hexane and dichloromethane) displayed the greatest inhibitory effects. Then, the coumarins xanthyletin (...
Source: Biotechnology Reports - November 30, 2019 Category: Biotechnology Source Type: research

Selective and sensitive colorimetric detection of platinum using Pseudomonas stutzeri mediated optimally synthesized antibacterial silver nanoparticles
Publication date: Available online 22 November 2019Source: Biotechnology ReportsAuthor(s): Megha P. Desai, Reshma V. Patil, Kiran D. PawarAbstractIn this work, Pseudomonas stutzeri was used for the optimum biogenic synthesis of antibacterial silver nanoparticles (AgNPs) which were applied for colorimetric detection of platinum ions (Pt+2). The optimum synthesis conditions were 2 mM AgNO3, pH 9 and incubation at 60 °C for 24 h. The FTIR spectra indicated that biomolecules such as amino acids, proteins or enzymes from P. stutzeri were involved in the synthesis of AgNPs in the size range of 10-50&thin...
Source: Biotechnology Reports - November 24, 2019 Category: Biotechnology Source Type: research

Draft genomes and initial characteriaztion of siderophore producing pseudomonads isolated from mine dump and mine drainage
Publication date: Available online 23 November 2019Source: Biotechnology ReportsAuthor(s): Marika Hofmann, Thomas Heine, Vivian Schulz, Sarah Hofmann, Dirk TischlerAbstractSiderophores are of high interest for biotechnological, pharmaceutical, agricultural and industrial applications. Although they are synthesized by various organisms, the yield is usually low which hindrances their suitability for broad range uses. Thus, it is necessary to identify novel producers and to increase the understanding of the biosynthesis pathways. Herein we report the isolation of two novel Pseudomonas strains and the identification of the ge...
Source: Biotechnology Reports - November 24, 2019 Category: Biotechnology Source Type: research

Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
Publication date: Available online 21 November 2019Source: Biotechnology ReportsAuthor(s): Mitsuki Sasaki, Akari Takagi, Shuhei Ota, Shigeyuki Kawano, Daisaku Sasaki, Munehiko AsayamaAbstractA novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG110 medium (BG11—N) with 2% CO2-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cult...
Source: Biotechnology Reports - November 22, 2019 Category: Biotechnology Source Type: research

Biogas production from recycled paper mill wastewater by UASB digester: optimal and mesophilic conditions
In this study, the AD experiment was performed in continuous mode with an effluent inlet flow rate equal to 1 L/h. Using the optimal OLR value 5.18 g COD/Ld and with an effluent 5,7 g COD/L, a biogas yield of 92 N mL/g COD removed (at normal temperature and pressure) was obtained during the RPMW anaerobic treatment. The reactor was operated at an optimal hydraulic retention time (HRT) of 15.14 h with a biogas production volume of the optimal value 62.5 L/d. These results indicate that RPMW can be effectively treated in a UASB reactor with the advantage of producing biogas. W...
Source: Biotechnology Reports - November 22, 2019 Category: Biotechnology Source Type: research

Household aluminum foil matte and bright side reflectivity measurements: Application to a photobioreactor light concentrator design
Publication date: Available online 20 November 2019Source: Biotechnology ReportsAuthor(s): Victor Pozzobon, Wendie Levasseur, Khanh-Van Do, Bruno Palpant, Patrick PerréAbstractThis work reports the design of a light concentrator intended to be used to cast uniform lighting over a photobioreactor. Household aluminum foils was chosen as reflective material to build the concentrator. This choice raised the question of which side to use. Thus measurements of household aluminum foil reflectivity spectra on both bright and matte sides were undergone. These measurements were done using an integrating sphere, over a 250&nda...
Source: Biotechnology Reports - November 21, 2019 Category: Biotechnology Source Type: research

Monitoring method for transgene expression in target tissue by blood sampling
In this study, we have developed a novel method to monitor transgene expression in tissues by blood sampling. We administered plasmid DNA (pDNA) encoding non-secretory form of firefly luciferase as a reporter gene and pDNA encoding secretable Gaussia princeps luciferase as a monitor gene simultaneously into mice. Good positive correlations were found between log-transgene expression of the reporter gene and the monitor gene in the treated muscle, between the monitor gene in the treated muscle and plasma, and consequently between the reporter gene in the treated muscle and the monitor gene in plasma after naked pDNA transfe...
Source: Biotechnology Reports - November 16, 2019 Category: Biotechnology Source Type: research

Hyper-production of Taxol from Aspergillus fumigatus, an endophytic fungus isolated from Taxus sp. of the Northern Himalayan region
Publication date: Available online 15 November 2019Source: Biotechnology ReportsAuthor(s): Pradeep Kumar, Balwant Singh, Vikram Thakur, Abhishek Thakur, Nandita Thakur, Deepak Pandey, Duni ChandAbstractTaxol® (generic name Paclitaxel) is a chemotherapeutic drug, effective against head, neck, breast, lung, bladder, ovary, and cervix cancers. Rising demands in chemotherapy and limited supply of natural taxol have ultimately increased the cost of the drug. Semi synthesis using taxol precursors is not able to meet the global supply and has intensified the need to find alternative ways of taxol production. In the present st...
Source: Biotechnology Reports - November 16, 2019 Category: Biotechnology Source Type: research

A simple, efficient and rapid screening technique for differentiating nitrile hydratase and nitrilase producing bacteria
Publication date: Available online 15 November 2019Source: Biotechnology ReportsAuthor(s): Ruchi Sahu, Anil Kumar Meghavarnam, Savitha JanakiramanAbstractNitrile hydrolyzing enzymes catalyze the hydration of nitrile compounds to corresponding amides and acids. Bacteria, isolated from soil samples were screened for nitrile hydrolyzing enzymes by simple dye based 96 well plate and nesslerization method. Bromothymol blue was used as an indicator for the detection of amides and acids based on colour change of the indicator dye from blue to dark green or yellow. The screening assay also differentiates between nitrile hydratase ...
Source: Biotechnology Reports - November 15, 2019 Category: Biotechnology Source Type: research

The current strategies and parameters for the enhanced microbial production of 2,3-butanediol
Publication date: Available online 13 November 2019Source: Biotechnology ReportsAuthor(s): Olivier Hakizimana, Emmanuel Matabaro, Byong H. LeeAbstract2,3-Butanediol (2,3-BD) is a propitious compound with many industrial uses ranging from rubber, fuels, and cosmetics to food additives. Its microbial production has especially attracted as an alternative way to the petroleum-based production. However, 2,3-BD production has always been hampered by low yields and high production costs. The enhanced production of 2,3-butanediol requires screening of the best strains and a systematic optimization of fermentation conditions. Moreo...
Source: Biotechnology Reports - November 15, 2019 Category: Biotechnology Source Type: research

Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4
In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum catalytic activity at 45 °C and pH-6.5 after 5.0 min. It presented greater stability within 0.1 M K2HPO4 buffer having pH-6.5 and showed 100% activity even after 1.0 h. It retained 83.6% and 45.0% activity at 40 °C after 1.0 and 3.0 h, respectively. The enzyme retained 90.0% activity at ˗20 °C even after 60 days. The molecular weight of enzyme was deduced to be 157.2 kDa as calculated using poly...
Source: Biotechnology Reports - November 13, 2019 Category: Biotechnology Source Type: research

Chemical design and bioefficacy screening of new insect growth regulators as potential insecticidal agents against Spodoptera littoralis (Boisd.)
Publication date: Available online 12 November 2019Source: Biotechnology ReportsAuthor(s): Antar A. Abdelhamid, M.M. Elwassimy, Safwat A. Aref, Mohamed A. GadAbstractThe 13 new compounds were chemically synthesized and their spectroscopic analysis was done to determine their chemical structure. All the compounds were screened for their insecticidal potential against Spodoptera littoralis (Boisd.). Among the tested compounds, the compound 13 was found to be the most potent. It displayed one fold more activity than a reported insect growth regulator, fenoxycarb. The other target compounds demonstrated weak to strong toxicolo...
Source: Biotechnology Reports - November 13, 2019 Category: Biotechnology Source Type: research

Genome-Wide Identification of Doublesex and Mab-3-Related Transcription Factor (DMRT) Genes in Nile Tilapia (Oreochromis niloticus)
Publication date: Available online 11 November 2019Source: Biotechnology ReportsAuthor(s): Imran zafar, Mohd Ashraf Rather, Bhushan C. DhandareAbstractDoublesex and Mab-3-related transcription factor (DMRT) gene family is extensively known for its contribution in sex determination and differentiation across phyla. Here we report the identification of five DM (doublesex and mab-3) domain genes in the Nile tilapia which includes DMRT1, DMRTa2, DMRT2a, DMRT2b and DMRT3a. The full-length sequence of DMRT genes ranges from 3526 (DMRTA2) to 1471bp (DMRT1) which encode putative proteins series from 469 to 372 amino acids. All the...
Source: Biotechnology Reports - November 12, 2019 Category: Biotechnology Source Type: research

Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines
Publication date: Available online 9 November 2019Source: Biotechnology ReportsAuthor(s): Sadegh Khorrami, Atefeh Zarepour, Ali ZarrabiAbstract"Black Peel Pomegranate" is a rare pomegranate cultivar that its specific features are still uncovered particularly in the bio-nano researches. The present study was organized to evaluate this pomegranate's potential in the biosynthesis of silver nanoparticles as well as bio-medical activities. According to the results, the pomegranate peel extract incredibly inhibited 100% of DPPH free radicals (EC50 = 5 μg/mL). This extract also induced more than ...
Source: Biotechnology Reports - November 10, 2019 Category: Biotechnology Source Type: research

Biochemical similarity between cultured chondrocytes and in situ chondrocytes by chemometric analysis from FTIR microspectroscopy
ConclusionThe biochemical composition and biodistribution analysis using FTIRM have been useful for comparing cultured chondrocytes and in situ chondrocytes. (Source: Biotechnology Reports)
Source: Biotechnology Reports - November 10, 2019 Category: Biotechnology Source Type: research

Protective responses of an engineered PspA recombinant antigen against Streptococcus pneumoniae
In this study, we developed two immunogenic antigens based on recombinant PspA proteins that including the different antigenic regions of PspA from both two families. The cross-reactivity of antibodies elicited against two PspA proteins PspAB1-5 and PspA4ABC and their role in complement deposition with three strains of pneumococci were tested. The protective effects of developed anti-PspA antibodies in mice in intranasal and intraperitoneal challenges were evaluated using a strain from clade 2. Sera from immunized mice with PspAB1-5 in comparison with PspA4ABC was able to deposit more C3 complement component on surface of ...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research

Biodegradation of phenanthrene as a model hydrocarbon: power display of a super-hydrophobic halotolerant enriched culture derived from a saline-sodic soil
In this study, after evaluating the degradation activity of enriched cultures from four crude oil-contaminated soils in mineral salt medium, the most efficient ones were selected for further studies. The chemical analysis of cell-free extract containing phenanthrene by HPLC suggested the superior enriched culture was able to degrade 87.66% of phenanthrene at the concentration of 40 mg L-1 within 10 days. This experiment was done under optimal conditions (37 °C, 10% salinity, and pH around 7 to 7.5). The 16S rRNA sequencing of isolates from this superior enriched culture indicated the highest simila...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research

Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application
Publication date: Available online 30 October 2019Source: Biotechnology ReportsAuthor(s): Josman Velasco, Bianca Oliva, Evandro José Mulinari, Leidy Patricia Quintero, Awana da Silva Lima, Aline Larissa Gonçalves, Thiago Augusto Gonçalves, André Damasio, Fabio Marcio Squina, Adriane Maria Ferreira Milagres, Asmaa Abdella, Mark R. Wilkins, Fernando SegatoAbstractXylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus (AF...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research

RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance
This study reports on the ability of a transgene-derived RNA hairpin, homologous to an overlapping region of the SACMV replication associated protein and putative virus suppressor of silencing protein (AC1/AC4), to confer tolerance in the CMD-susceptible model cassava cultivar 60444. Three of the fourteen transgenic lines expressing SACMV AC1/AC4 hairpin-derived siRNAs showed decreased symptoms and viral loads compared to untransformed control plants. Expression of SACMV AC1/AC4 homologous siRNAs showed that this tolerance is most likely associated with post-transcriptional gene silencing of the virus. This is the first re...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research

In vitro proliferation and differentiation of canine bone marrow derived mesenchymal stem cells over hydroxyl functionalized CNT substrates
Publication date: Available online 27 October 2019Source: Biotechnology ReportsAuthor(s): A.P. Madhusoodan, Kinsuk Das, Bhabesh Mili, Kuldeep Kumar, Ajay Kumar, A.C. Saxena, Praveen Singh, Triveni Dutt, Sadhan BagAbstractNanotopography of culture substrate acts as a positive cue in cell-biomaterial based tissue regeneration. Considering the potentiality of carbon nanotubes (CNTs) this study was designed to evaluate its two functionalized form by an in vitro culture condition using canine mesenchymal stem cells as cellular model. Cells were isolated and its behaviour, proliferation and differentiation processes were elucida...
Source: Biotechnology Reports - October 29, 2019 Category: Biotechnology Source Type: research

Nematicidal Activity of Thermostable Alkaline Protease Produced by Saccharomonospora viridis strain Hw G550
Publication date: Available online 25 October 2019Source: Biotechnology ReportsAuthor(s): Osama M. Darwesh, Ahmad S. El-Hawary, Usama S. El Kelany, Gamal M. El-SherbinyAbstractApplication of thermostable alkaline protease to control the harmful nematodes was investigated in the current study. A total of 14 proteolytic actinomycetes were isolated from Egyptian harsh environments. Out of them, isolate G550 exhibited the highest proteolytic activity (528.9 U/ml). Protease from isolate G550 exhibited high nematicidal activity against M. incognita under laboratory conditions and caused hydrolysis of J2S cuticle. This isolate wa...
Source: Biotechnology Reports - October 26, 2019 Category: Biotechnology Source Type: research

Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect
Publication date: Available online 25 October 2019Source: Biotechnology ReportsAuthor(s): C.M. López-Domínguez, M.O. Ramírez-Sucre, I.M. Rodríguez-BuenfilAbstractIn the present work the effect of three factors: pH, temperature and type of microorganism using a factorial design 32x2 was evaluated on: growth, total cellulases hydrolytic activity (FPases), endoglucanases hydrolytic activity (CMCases), free reducing sugars (FRS), glucose, sucrose, and alcohol production using a culture medium based on mineral salts added with cladodes flour of Opuntia ficus-indica at 20% as the unique carbon source....
Source: Biotechnology Reports - October 26, 2019 Category: Biotechnology Source Type: research

In silico analysis of functional linkage among arsenic induced MATE genes in rice
Publication date: Available online 24 October 2019Source: Biotechnology ReportsAuthor(s): Snigdhamayee Seth, Sandip Debnath, NR ChakrabortyAbstractMATE genes play an important role in cellular detoxification processes. Nine MATE genes were identified by a transcriptomics study previously. Candidate gene prioritization was done where 29 new genes were found to interact with 09 guide genes. Therefore, a total of 38 genes were analyzed here to predict a concise model by gene prioritization study. Those genes were analyzed further in Rice Interactions Viewer programme, and based on high ICV, 10 new genes were found to interact...
Source: Biotechnology Reports - October 25, 2019 Category: Biotechnology Source Type: research

Mutagenicity, genotoxicity and oxidative stress induced by pesticide industry wastewater using bacterial and plant bioassays
Publication date: Available online 24 October 2019Source: Biotechnology ReportsAuthor(s): Mohammad Tarique Zeyad, Kumar Murugan, Abdul MalikAbstractAtomic absorption spectrophotometer and gas chromatography analysis revealed the presence of heavy metals, organochlorine and organophosphate pesticides in industrial wastewater. XAD, Dichloromethane and n-Hexane extracted wastewater were analysed for genotoxic potential using Ames Salmonella/mammalian microsome test. The XAD concentrated sample displayed remarkable mutagenic activity compared to solvent assisted liquid–liquid extraction. Strain TA98 was found utmost sens...
Source: Biotechnology Reports - October 25, 2019 Category: Biotechnology Source Type: research

Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)
Publication date: Available online 5 October 2019Source: Biotechnology ReportsAuthor(s): Pavel A. Levashov, Darya A. Matolygina, Oxana A. Dmitrieva, Ekaterina D. Ovchinnikova, Irina Yu. Adamova, Nataliya V. Karelina, Vladimir A. Nelyub, Nikolay L. Eremeev, Andrey V. LevashovAbstractChemical modification of lysozyme was carried out using benzaldehyde and anisaldehyde. It was shown that chemical modification affects only 1-2 amino groups of the protein molecule which does not prevent further covalent immobilization of lysozyme using the remaining free amino groups. The bacteriolytic activity of lysozyme is preserved after ch...
Source: Biotechnology Reports - October 6, 2019 Category: Biotechnology Source Type: research

Red light controlled callus morphogenetic patterns and secondary metabolites production in Withania somnifera L
This study concludes that red light treatment was optimum for maximum biomass accumulation and anti-oxidant activity in calli of W. somnifera. (Source: Biotechnology Reports)
Source: Biotechnology Reports - September 20, 2019 Category: Biotechnology Source Type: research

Enzymatic, antimicrobial, and leishmanicidal bioactivity of Gram-negative bacteria strains from the midgut of Lutzomyia evansi, an insect vector of leishmaniasis in Colombia
Publication date: Available online 18 September 2019Source: Biotechnology ReportsAuthor(s): Rafael J. Vivero, Gustavo Bedoya Mesa, Sara M. Robledo, Claudia Ximena Moreno Herrera, Gloria Cadavid-RestrepoAbstractKnowledge regarding new compounds, peptides, and/or secondary metabolites secreted by bacteria isolated from the intestine of phebotominae has the potential to control insect vectors and pathogens (viruses, bacteria, and parasites) transmitted by them. In this respect, twelve Gram-negative bacteria isolated from the intestine of Lutzomyia evansi were selected and screened for their enzymatic, antimicrobial, and leish...
Source: Biotechnology Reports - September 19, 2019 Category: Biotechnology Source Type: research

Endophytic Bacillus megaterium BM18-2 mutated for cadmium accumulation and improving plant growth in Hybrid Pennisetum
Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): Juanzi Wu, Nehal Kamal, Huanhuan Hao, Zhiwei Liu, Chen Qian, Yuhe Shao, Xiaoxian Zhong, Bin XuAbstractHybrid Pennisetum (Pennisetum americanum × P. purpureum Schumach L.) is considered as long, rapidly growing perennial C4 bunchgrasses and promising plant for heavy metals phytoremediation due to its high biomass productivity, high resistance to environmental stress, pests and diseases. Metal bioavailability level is the most important parameters to measure efficiency of phytoremediation. Recently, in order to improve metal acc...
Source: Biotechnology Reports - September 13, 2019 Category: Biotechnology Source Type: research

Evaluation of Cytotoxicity, Biochemical Profile and Yield Components of Groundnut Plants Treated with Nano-Selenium
Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): Hebat-Allah A. Hussein, Osama M. Darwesh, Bahaa B. Mekki, Salwa M. El-HalloutyAbstractKnowledge about the risks of the nanoparticles application on the plant development and human health is still limited. Different concentrations of nano-selenium (0, 20 and 40 ppm) were applied to three different Egyptian groundnut (Arachis hypogaea l.) cultivars; (NC, Gregory and Giza 6) under sandy soil conditions at vegetative growth stage to investigate their effects on yield components, protein profile, fatty acids composition, total ant...
Source: Biotechnology Reports - September 13, 2019 Category: Biotechnology Source Type: research

Biocompatible synthesis of reduced graphene oxide from Euphorbia heterophylla (L.) and their in-vitro cytotoxicity against human cancer cell lines
Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): K. Lingaraju, H. Raja Naika, G. Nagaraju, H. NagabhushanaAbstractFacile and biocompatible synthesis of reduced graphene oxide from graphene oxide as a precursor and aqueous leaves extract of Euphorbia heterophylla (L.), act as a reducing /capping /stabilizing agent by green chemistry approaches. The obtained product was analyzed by Ultraviolet-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and Scanning electron Microscopy (SEM). In addition to this, the sig...
Source: Biotechnology Reports - September 13, 2019 Category: Biotechnology Source Type: research

Effective production of succinic acid from coconut water (Cocos nucifera) by metabolically engineered Escherichia coli with overexpression of Bacillus subtilis pyruvate carboxylase
Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): Ayobami Matthew Olajuyin, Maohua Yang, Anders Thygesen, Jiangnan Tian, Tingzhen Mu, Jianmin XingAbstractSuccinic acid is an important acid which is used in medicine and pharmaceutical companies. Metabolically engineered Escherichia coli strain was used for the effective production of succinic acid using Cocos nucifera water, which contained 5.00 ± 0.02 g/L glucose, 6.10 ± 0.01 g /L fructose and 6.70 ± 0.02 g /L sucrose. Fermentation of C. nucifera wa...
Source: Biotechnology Reports - September 13, 2019 Category: Biotechnology Source Type: research

Bioorganic synthesis, characterization and evaluation of a natural phenolic lipid
Publication date: Available online 12 September 2019Source: Biotechnology ReportsAuthor(s): Juliya Johny, Venkateshwarlu Kontham, Dileep Veeragoni, Sunil Misra, Shiva Shanker KakiAbstractThe first synthesis of a phenolic natural monoacylglycerol (1- [11-(ferulyloxy) undecanoyl)] glycerol) was carried out by bioorganic synthesis starting from ferulic acid. The synthetic route of the target lipidic compound was designed involving a chemo-enzymatic approach using immobilized Candida antarctica lipase as biocatalyst in two of the steps conducted in organic medium. The prepared lipidic compound was characterized by using spectr...
Source: Biotechnology Reports - September 13, 2019 Category: Biotechnology Source Type: research

Immobilization of pectinase on chitosan-magnetic particles: influence of particle preparation protocol on enzyme properties for fruit juice clarification
Publication date: Available online 24 August 2019Source: Biotechnology ReportsAuthor(s): Lucas Dal Magro, Kelly Silva de Moura, Betina Elys Backes, Eliana Weber de Menezes, Edilson Valmir Benvenutti, Sabrina Nicolodi, Manuela P. Klein, Roberto Fernandez-Lafuente, Rafael C. RodriguesAbstractMagnetic-chitosan particles were prepared following three different protocols enabling the preparation of particles with different sizes – nano (Nano-CMag, Micro (Micro-CMag) and Macro (Macro-CMag) – and used for pectinase immobilization and clarification of grape, apple and orange juices. The particle size had a great effect...
Source: Biotechnology Reports - August 25, 2019 Category: Biotechnology Source Type: research

Production of metabolites of the anti-cancer drug noscapine using a P450BM3 mutant library
In this study, a library of P450BM3 mutants was produced using site-directed mutagenesis and the enzymes screened for metabolism of the opium poppy alkaloid noscapine, a drug with anticancer activity. Of the 18 enzyme mutants screened, 12 showed an ability to metabolise noscapine that was not present in the wild-type enzyme. Five noscapine metabolites were detected by LC-MS/MS, with the major metabolite for all mutants being N-demethylated noscapine. The highest observed regioselectivity for N-demethylation was 88%. Two hydroxylated metabolites, a catechol and two C-C cleavage products were also detected. P450-mediated pro...
Source: Biotechnology Reports - August 25, 2019 Category: Biotechnology Source Type: research