Comparative Genomics of Clostridioides difficile
Adv Exp Med Biol. 2024;1435:199-218. doi: 10.1007/978-3-031-42108-2_10.ABSTRACTClostridioides difficile, a Gram-positive spore-forming anaerobic bacterium, has rapidly emerged as the leading cause of nosocomial diarrhoea in hospitals. The availability of large numbers of genome sequences, mainly due to the use of next-generation sequencing methods, has undoubtedly shown their immense advantages in the determination of C. difficile population structure. The implementation of fine-scale comparative genomic approaches has paved the way for global transmission and recurrence studies, as well as more targeted studies, such as t...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Sandra Janezic Julian R Garneau Marc Monot Source Type: research
Ribotypes and New Virulent Strains Across Europe
Adv Exp Med Biol. 2024;1435:151-168. doi: 10.1007/978-3-031-42108-2_8.ABSTRACTClostridioides (formerly Clostridium) difficile is a major bacterial cause of post-antibiotic diarrhoea. The epidemiology of C. difficile infections (CDIs) has dramatically changed since the early 2000s, with an increasing incidence and severity across Europe. This trend is partly due to the emergence and rapid worldwide spread of the hypervirulent and epidemic PCR ribotype 027. Profiles of patients with CDI have also evolved, with description of community-acquired (CA) infections in patients with no traditional risk factors for CDI. However, epi...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Jeanne Couturier Kerrie Davies Fr édéric Barbut Source Type: research
Comparative Genomics of Clostridioides difficile
Adv Exp Med Biol. 2024;1435:199-218. doi: 10.1007/978-3-031-42108-2_10.ABSTRACTClostridioides difficile, a Gram-positive spore-forming anaerobic bacterium, has rapidly emerged as the leading cause of nosocomial diarrhoea in hospitals. The availability of large numbers of genome sequences, mainly due to the use of next-generation sequencing methods, has undoubtedly shown their immense advantages in the determination of C. difficile population structure. The implementation of fine-scale comparative genomic approaches has paved the way for global transmission and recurrence studies, as well as more targeted studies, such as t...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Sandra Janezic Julian R Garneau Marc Monot Source Type: research
Ribotypes and New Virulent Strains Across Europe
Adv Exp Med Biol. 2024;1435:151-168. doi: 10.1007/978-3-031-42108-2_8.ABSTRACTClostridioides (formerly Clostridium) difficile is a major bacterial cause of post-antibiotic diarrhoea. The epidemiology of C. difficile infections (CDIs) has dramatically changed since the early 2000s, with an increasing incidence and severity across Europe. This trend is partly due to the emergence and rapid worldwide spread of the hypervirulent and epidemic PCR ribotype 027. Profiles of patients with CDI have also evolved, with description of community-acquired (CA) infections in patients with no traditional risk factors for CDI. However, epi...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Jeanne Couturier Kerrie Davies Fr édéric Barbut Source Type: research
Comparative Genomics of Clostridioides difficile
Adv Exp Med Biol. 2024;1435:199-218. doi: 10.1007/978-3-031-42108-2_10.ABSTRACTClostridioides difficile, a Gram-positive spore-forming anaerobic bacterium, has rapidly emerged as the leading cause of nosocomial diarrhoea in hospitals. The availability of large numbers of genome sequences, mainly due to the use of next-generation sequencing methods, has undoubtedly shown their immense advantages in the determination of C. difficile population structure. The implementation of fine-scale comparative genomic approaches has paved the way for global transmission and recurrence studies, as well as more targeted studies, such as t...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Sandra Janezic Julian R Garneau Marc Monot Source Type: research
Ribotypes and New Virulent Strains Across Europe
Adv Exp Med Biol. 2024;1435:151-168. doi: 10.1007/978-3-031-42108-2_8.ABSTRACTClostridioides (formerly Clostridium) difficile is a major bacterial cause of post-antibiotic diarrhoea. The epidemiology of C. difficile infections (CDIs) has dramatically changed since the early 2000s, with an increasing incidence and severity across Europe. This trend is partly due to the emergence and rapid worldwide spread of the hypervirulent and epidemic PCR ribotype 027. Profiles of patients with CDI have also evolved, with description of community-acquired (CA) infections in patients with no traditional risk factors for CDI. However, epi...
Source: Advances in Experimental Medicine and Biology - January 4, 2024 Category: Research Authors: Jeanne Couturier Kerrie Davies Fr édéric Barbut Source Type: research
Survey on resistance occurrence for F4+ and F18+ enterotoxigenic Escherichia coli (ETEC) among pigs reared in Central Italy regions
AbstractPorcine Post Weaning Diarrhoea (PWD) is one of the most important swine disease worldwide, caused by EnterotoxigenicEscherichia coli (ETEC) strains able to provoke management, welfare and sanitary issues. ETEC is determined by proteinaceous surface appendages. Numerous studies conducted by now in pigs have demonstrated, at the enterocytes level, that, the genesmucin 4 (MUC4) andfucosyltransferase (FUT1), coding for ETEC F4 and F18 receptors respectively, can be carriers of single nucleotide polymorphisms (SNPs) associated with natural resistance/susceptibility to PWD. The latter aspect was investigated in this stud...
Source: Veterinary Research Communications - January 4, 2024 Category: Veterinary Research Source Type: research
Temporal changes in the positivity rate of common enteric viruses among paediatric admissions in coastal Kenya, during the COVID-19 pandemic, 2019 –2022
ConclusionOur study finds that in 2020 the transmission of common enteric viruses, especially RVA and astrovirus, in Kilifi Kenya may have been disrupted due to the COVID-19 NPIs. After 2020, local enteric virus transmission patterns appeared to return to pre-pandemic levels coinciding with the removal of most of the government COVID-19 NPIs. (Source: Gut Pathogens)
Source: Gut Pathogens - January 4, 2024 Category: Microbiology Source Type: research
Indirubin mediates adverse intestinal reactions in guinea pigs by downregulating the expression of AchE through AhR
This study showed that the inhibition of AchE expression by indirubin plays a key role in the occurrence of adverse reactions to indirubin and that the underlying mechanism is related to AhR-mediated AchE downregulation.PMID:38164702 | DOI:10.1080/00498254.2023.2297745 (Source: Xenobiotica)
Source: Xenobiotica - January 2, 2024 Category: Research Authors: Xiaoting Xu Reham Taha Chenghan Chu Li Xiao Tao Wang Xinzhi Wang Xin Huang Zhenzhou Jiang Lixin Sun Source Type: research
Indirubin mediates adverse intestinal reactions in guinea pigs by downregulating the expression of AchE through AhR
This study showed that the inhibition of AchE expression by indirubin plays a key role in the occurrence of adverse reactions to indirubin and that the underlying mechanism is related to AhR-mediated AchE downregulation.PMID:38164702 | DOI:10.1080/00498254.2023.2297745 (Source: Xenobiotica)
Source: Xenobiotica - January 2, 2024 Category: Research Authors: Xiaoting Xu Reham Taha Chenghan Chu Li Xiao Tao Wang Xinzhi Wang Xin Huang Zhenzhou Jiang Lixin Sun Source Type: research
Recombinantly expressed virus-like particles (VLPs) of canine circovirus for development of an indirect ELISA
In this study, full length CanineCV capsid gene was expressed inEscherichia coli followed by two-step purification process to yield soluble capsid protein in high concentration. Transmission electron microscopy (TEM) confirmed the capsid antigen self-assembled into 17 –20 nm VLPs in glutathione S-transferase (GST) buffer, later utilised to develop an indirect enzyme-linked immunosorbent assay (iELISA). The respective sensitivity and specificity of the proposed iELISA were 94.10% and 88.40% compared with those obtained from Western blot. The mean OD450 value for western blot positive samples was 1.22 (range 0.12 –3.39)...
Source: Veterinary Research Communications - January 2, 2024 Category: Veterinary Research Source Type: research
Chemoprofiling and antimicrobial activity of medicinal herbs used in the treatment of inflammatory bowel disease
In conclusion. the plants' extracts and an equal mixture of them showed a narrow spectrum of antimicrobial activity against S. aureus, K. pneumoniae, P. mirabilis, P. aeruginosa and C. albicans.PMID:38158691 | DOI:10.14715/cmb/2023.69.13.6 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - December 30, 2023 Category: Molecular Biology Authors: Ali Alshahrani Abuzer Ali Sayed F Abdelwahab Source Type: research
Chemoprofiling and antimicrobial activity of medicinal herbs used in the treatment of inflammatory bowel disease
In conclusion. the plants' extracts and an equal mixture of them showed a narrow spectrum of antimicrobial activity against S. aureus, K. pneumoniae, P. mirabilis, P. aeruginosa and C. albicans.PMID:38158691 | DOI:10.14715/cmb/2023.69.13.6 (Source: Mol Biol Cell)
Source: Mol Biol Cell - December 30, 2023 Category: Molecular Biology Authors: Ali Alshahrani Abuzer Ali Sayed F Abdelwahab Source Type: research
Nintedanib could potentially lead to improvements in anti-melanoma differentiation-associated 5 dermatomyositis-associated interstitial lung disease
CONCLUSIONS: Nintedanib may be effective for improving clinical symptoms, laboratory parameters, lung lesions, and survival in anti-MDA5+ DM. Diarrhoea was the most common adverse event associated with nintedanib, although the drug was well tolerated by most patients.PMID:38153137 | DOI:10.55563/clinexprheumatol/c0i032 (Source: Clinical and Experimental Rheumatology)
Source: Clinical and Experimental Rheumatology - December 28, 2023 Category: Rheumatology Authors: Xixia Chen Wei Jiang Qiwen Jin Sang Lin Lu Zhang Qinglin Peng Hongliang Sun Yuli Wang Xin Lu Aiping Song Guochun Wang Yongpeng Ge Source Type: research
