Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Effect of in-water administration of quorum system inhibitors in broilers' productive performance and intestinal microbiome in a mild necrotic enteritis challenge
In this study, the peptide-containing cell-free spent media (CFSM) from Enterococcus faecium was tested in NE challenged broilers in two battery cage and one floor pen studies. Results showed a significant reduction of NE mortality. Metagenomic sequencing of the jejunum microbiome revealed no impact of the CFSM on the microbial community, and growth of C. perfringens was unaffected by CFSM in vitro. The expression of QS-controlled virulence genes netB, plc and pfoA was found to be significantly repressed by CFSM during the mid-logarithmic stage of C. perfringens growth and this corresponded with a significant decrease in h...
Source: Avian Pathology - July 24, 2023 Category: Pathology Authors: A S Jaramillo-Jaramillo T J D Coulson C Hofacre M Jones L O'Neill N Nguyen A Labbe Source Type: research
Molecular characterization and function of JAK/STAT pathway in IPEC-J2 cells during Clostridium perfringens beta2 toxin stimulation
AbstractIntestinal infection withC. perfringens is responsible for outbreaks of diarrhea in piglets. Janus kinase / signal transducer and activator of transcription (JAK/STAT) is a vital signaling pathway that regulates cellular activity and inflammatory response, closely correlated with multiple diseases development and advances. Currently, the potential effect of JAK/STAT onC. perfringens beta2 (CPB2) treatment on porcine intestinal epithelial (IPEC-J2) cells has not been explored. The expression of JAK/STAT genes or proteins in IPEC-J2 cells induced by CPB2 were observed by qRT-PCR and Western blot, and further used WP1...
Source: Veterinary Research Communications - July 12, 2023 Category: Veterinary Research Source Type: research
Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021
Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Our study investigated MLST, phenotypic antimicrobial resistance profiles, and resistance genes among isolates from human, animal and food. 186 C. perfringens isolates were obtained from nine provinces in China between 2013 and 2021. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/...
Source: Frontiers in Microbiology - July 6, 2023 Category: Microbiology Source Type: research
Diet-induced changes in fecal microbiota composition and diversity in dogs (Canis lupus familiaris): A comparative study of BARF-type and commercial diets
CONCLUSIONS: This study is the first one carried out in dogs from Colombia that seeks to identify changes in the intestinal microbiota concerning natural BARF type diet and commercial diet using a metabarcoding approach. Important differences were identified in terms of richness, diversity, and differentially enriched bacteria in each of the diets. The microbiota of dogs fed the BARF diet was characterized by higher richness and diversity compared to the commercial diet. However, it was identified that BARF-fed dogs can potentially acquire more opportunistic infections by pathogens of importance such as C. perfringens. Mos...
Source: Comparative immunology, microbiology and infectious diseases. - June 25, 2023 Category: Allergy & Immunology Authors: Sergio Casta ñeda Gineth Ariza Andres Rinc ón-Riveros Marina Mu ñoz Juan David Ram írez Source Type: research
Clostridium perfringens epsilon prototoxin mutant rpETX < sub > Y30A/Y71A/H106P/Y196A < /sub > as a vaccine candidate against enterotoxemia
Vaccine. 2023 Jun 23:S0264-410X(23)00719-3. doi: 10.1016/j.vaccine.2023.06.044. Online ahead of print.ABSTRACTEpsilon toxin (ETX) is secreted by Clostridium perfringens (C. perfringens)as a relatively inactive prototoxin (pETX), which is enzymatically activated to ETX by removing carboxy-terminal and amino-terminal peptides. Genetically engineered ETX mutants have been shown to function as potential vaccine candidates in the prevention of the enterotoxemia caused by C. perfringens. In the present study, two recombinant site-directed mutants of pETX, rpETXY30A/Y71A/H106P/Y196A (rpETXm41) and rpETXY30A/H106P/Y196A/F199E (rpE...
Source: Vaccine - June 25, 2023 Category: Allergy & Immunology Authors: Jige Du Tuanjie Wang Lei Xu Cong Wang Ying Liu Chenfan Pan Xiaoyun Chen Zhen Zhu Yufeng Luo Chunsheng Yin Source Type: research
Diet-induced changes in fecal microbiota composition and diversity in dogs (Canis lupus familiaris): A comparative study of BARF-type and commercial diets
CONCLUSIONS: This study is the first one carried out in dogs from Colombia that seeks to identify changes in the intestinal microbiota concerning natural BARF type diet and commercial diet using a metabarcoding approach. Important differences were identified in terms of richness, diversity, and differentially enriched bacteria in each of the diets. The microbiota of dogs fed the BARF diet was characterized by higher richness and diversity compared to the commercial diet. However, it was identified that BARF-fed dogs can potentially acquire more opportunistic infections by pathogens of importance such as C. perfringens. Mos...
Source: Comparative immunology, microbiology and infectious diseases. - June 25, 2023 Category: Allergy & Immunology Authors: Sergio Casta ñeda Gineth Ariza Andres Rinc ón-Riveros Marina Mu ñoz Juan David Ram írez Source Type: research
Clostridium perfringens epsilon prototoxin mutant rpETX < sub > Y30A/Y71A/H106P/Y196A < /sub > as a vaccine candidate against enterotoxemia
Vaccine. 2023 Jun 23:S0264-410X(23)00719-3. doi: 10.1016/j.vaccine.2023.06.044. Online ahead of print.ABSTRACTEpsilon toxin (ETX) is secreted by Clostridium perfringens (C. perfringens)as a relatively inactive prototoxin (pETX), which is enzymatically activated to ETX by removing carboxy-terminal and amino-terminal peptides. Genetically engineered ETX mutants have been shown to function as potential vaccine candidates in the prevention of the enterotoxemia caused by C. perfringens. In the present study, two recombinant site-directed mutants of pETX, rpETXY30A/Y71A/H106P/Y196A (rpETXm41) and rpETXY30A/H106P/Y196A/F199E (rpE...
Source: Vaccine - June 25, 2023 Category: Allergy & Immunology Authors: Jige Du Tuanjie Wang Lei Xu Cong Wang Ying Liu Chenfan Pan Xiaoyun Chen Zhen Zhu Yufeng Luo Chunsheng Yin Source Type: research
Diet-induced changes in fecal microbiota composition and diversity in dogs (Canis lupus familiaris): A comparative study of BARF-type and commercial diets
CONCLUSIONS: This study is the first one carried out in dogs from Colombia that seeks to identify changes in the intestinal microbiota concerning natural BARF type diet and commercial diet using a metabarcoding approach. Important differences were identified in terms of richness, diversity, and differentially enriched bacteria in each of the diets. The microbiota of dogs fed the BARF diet was characterized by higher richness and diversity compared to the commercial diet. However, it was identified that BARF-fed dogs can potentially acquire more opportunistic infections by pathogens of importance such as C. perfringens. Mos...
Source: Comparative immunology, microbiology and infectious diseases. - June 25, 2023 Category: Allergy & Immunology Authors: Sergio Casta ñeda Gineth Ariza Andres Rinc ón-Riveros Marina Mu ñoz Juan David Ram írez Source Type: research
Characterization and heterologous expression of plasmalogen synthase MeHAD from Megasphaera elsdenii
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jun 20:159358. doi: 10.1016/j.bbalip.2023.159358. Online ahead of print.ABSTRACTPlasmalogens (Pls) are vinyl-ether bond-containing glycerophospholipids or glycosyl diradyl glycerols, and are of great importance in the physiological functions and stability of cell membrane. Here, we identified and characterized that the plasmalogen synthase MeHAD from anaerobic Megasphaera elsdenii was responsible for vinyl-ether bond formation. Different from the 2-hydroxyacyl-CoA dehydratase (HAD) family plasmalogen synthase PlsA-PlsR which are encoded by two genes in Clostridium perfringens...
Source: Biochimica et Biophysica Acta - June 22, 2023 Category: Biochemistry Authors: Furong Zhang Zhaonan Yang Yulong Zhou Binqiang Wang Zheming Xie Ning Yu Jie Zhao Howard Goldfine Shang Dai Genlin Zhang Bing Tian Source Type: research
Characterization and heterologous expression of plasmalogen synthase MeHAD from Megasphaera elsdenii
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jun 20:159358. doi: 10.1016/j.bbalip.2023.159358. Online ahead of print.ABSTRACTPlasmalogens (Pls) are vinyl-ether bond-containing glycerophospholipids or glycosyl diradyl glycerols, and are of great importance in the physiological functions and stability of cell membrane. Here, we identified and characterized that the plasmalogen synthase MeHAD from anaerobic Megasphaera elsdenii was responsible for vinyl-ether bond formation. Different from the 2-hydroxyacyl-CoA dehydratase (HAD) family plasmalogen synthase PlsA-PlsR which are encoded by two genes in Clostridium perfringens...
Source: Mol Biol Cell - June 22, 2023 Category: Molecular Biology Authors: Furong Zhang Zhaonan Yang Yulong Zhou Binqiang Wang Zheming Xie Ning Yu Jie Zhao Howard Goldfine Shang Dai Genlin Zhang Bing Tian Source Type: research
Characterization and heterologous expression of plasmalogen synthase MeHAD from Megasphaera elsdenii
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jun 20:159358. doi: 10.1016/j.bbalip.2023.159358. Online ahead of print.ABSTRACTPlasmalogens (Pls) are vinyl-ether bond-containing glycerophospholipids or glycosyl diradyl glycerols, and are of great importance in the physiological functions and stability of cell membrane. Here, we identified and characterized that the plasmalogen synthase MeHAD from anaerobic Megasphaera elsdenii was responsible for vinyl-ether bond formation. Different from the 2-hydroxyacyl-CoA dehydratase (HAD) family plasmalogen synthase PlsA-PlsR which are encoded by two genes in Clostridium perfringens...
Source: Biochimica et Biophysica Acta - June 22, 2023 Category: Biochemistry Authors: Furong Zhang Zhaonan Yang Yulong Zhou Binqiang Wang Zheming Xie Ning Yu Jie Zhao Howard Goldfine Shang Dai Genlin Zhang Bing Tian Source Type: research
