Optimization and scale-up of Clostridium perfringens type D culture and epsilon-toxin production: Effects of stirring, glucose and pH adjustment
Toxicon. 2023 Sep 26:107302. doi: 10.1016/j.toxicon.2023.107302. Online ahead of print.ABSTRACTThe effects of some main bacteria culture parameters including mixing rate, glucose (GC) concentration, steps of GC addition, and steps of pH adjustment on both C. perfringens bacteria growth and its epsilon toxin production in a bench-scale 20-L glass carboy were investigated. The optimized mixing rate of 300 rpm, GC concentration of 4 g L-1, and 3-step addition of GC resulted in the bacteria and toxin concentrations of 0.16 g L-1 and 330 ng mL-1, respectively. Also, the induction of a pH shock at the reaction time of 180 min le...
Source: Toxicon - September 28, 2023 Category: Toxicology Authors: Parvaneh Esmaeilnejad Ahranjani Behjat Majidi Alireza Paradise Mona Hasanzadeh Source Type: research
Optimization and scale-up of Clostridium perfringens type D culture and epsilon-toxin production: Effects of stirring, glucose and pH adjustment
Toxicon. 2023 Sep 26:107302. doi: 10.1016/j.toxicon.2023.107302. Online ahead of print.ABSTRACTThe effects of some main bacteria culture parameters including mixing rate, glucose (GC) concentration, steps of GC addition, and steps of pH adjustment on both C. perfringens bacteria growth and its epsilon toxin production in a bench-scale 20-L glass carboy were investigated. The optimized mixing rate of 300 rpm, GC concentration of 4 g L-1, and 3-step addition of GC resulted in the bacteria and toxin concentrations of 0.16 g L-1 and 330 ng mL-1, respectively. Also, the induction of a pH shock at the reaction time of 180 min le...
Source: Toxicon - September 28, 2023 Category: Toxicology Authors: Parvaneh Esmaeilnejad Ahranjani Behjat Majidi Alireza Paradise Mona Hasanzadeh Source Type: research
Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression of Clostridium perfringens
This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens infection by inhibiting the virulence-associated traits and virulence genes expression. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - September 25, 2023 Category: Microbiology Source Type: research
Effects of Eimeria maxima infection doses on growth performance and gut health in dual-infection model of necrotic enteritis in broiler chickens
In conclusion, increasing the infection doses of EM significantly aggravated the severity of NE and exerted negative impact on intestinal health from 5 to 7 dpi. (Source: Frontiers in Physiology)
Source: Frontiers in Physiology - September 20, 2023 Category: Physiology Source Type: research
Digital PCR: a tool in clostridial mutant selection and detection
Appl Microbiol Biotechnol. 2023 Sep 14. doi: 10.1007/s00253-023-12779-8. Online ahead of print.ABSTRACTThe ClosTron mutagenesis system has enabled researchers to efficiently edit the clostridial genome. Since site-specific insertion of the mobile ClosTron insert may cause errors, validation is key. In this paper we describe the use of digital PCR (dPCR) as an alternative tool in selecting clostridial mutant strains. Clostridium perfringens chitinase mutant strains were constructed in which the mobile ClosTron intron was inserted into one of the chitinase genes. On-target insertion of the mobile intron was validated through...
Source: Applied Microbiology and Biotechnology - September 13, 2023 Category: Microbiology Authors: Evelien Dierick Chana Callens Ward De Spiegelaere Richard Ducatelle Filip Van Immerseel Evy Goossens Source Type: research
Digital PCR: a tool in clostridial mutant selection and detection
Appl Microbiol Biotechnol. 2023 Sep 14. doi: 10.1007/s00253-023-12779-8. Online ahead of print.ABSTRACTThe ClosTron mutagenesis system has enabled researchers to efficiently edit the clostridial genome. Since site-specific insertion of the mobile ClosTron insert may cause errors, validation is key. In this paper we describe the use of digital PCR (dPCR) as an alternative tool in selecting clostridial mutant strains. Clostridium perfringens chitinase mutant strains were constructed in which the mobile ClosTron intron was inserted into one of the chitinase genes. On-target insertion of the mobile intron was validated through...
Source: Applied Microbiology and Biotechnology - September 13, 2023 Category: Microbiology Authors: Evelien Dierick Chana Callens Ward De Spiegelaere Richard Ducatelle Filip Van Immerseel Evy Goossens Source Type: research
Diversity and probiotic characterisation of yeast isolates in the bovine gastrointestinal tract
The objective of this study was to isolate and identify yeasts present in the bovine feces and evaluate their probiotic potential together with strains previously isolated from the rumen (preliminary study). A total of 193 isolates were studied, including 139 isolates (19 species) from fecal samples from 11 different animals (Bos taurus and Bos indicus) and 54 strains previously isolated from rumen fluid (Bos taurus). The yeast population in the feces ranged from 3.51 to 4.99 log CFU/g, with Candida pararugosa being the most abundant (isolated from the feces of six samples analysed). Isolates were selected that had negativ...
Source: Antonie van Leeuwenhoek - August 31, 2023 Category: Microbiology Authors: G F C Sales B F Carvalho R F Schwan M N Pereira C L S Ávila Source Type: research
Diversity and probiotic characterisation of yeast isolates in the bovine gastrointestinal tract
The objective of this study was to isolate and identify yeasts present in the bovine feces and evaluate their probiotic potential together with strains previously isolated from the rumen (preliminary study). A total of 193 isolates were studied, including 139 isolates (19 species) from fecal samples from 11 different animals (Bos taurus and Bos indicus) and 54 strains previously isolated from rumen fluid (Bos taurus). The yeast population in the feces ranged from 3.51 to 4.99 log CFU/g, with Candida pararugosa being the most abundant (isolated from the feces of six samples analysed). Isolates were selected that had negativ...
Source: Antonie van Leeuwenhoek - August 31, 2023 Category: Microbiology Authors: G F C Sales B F Carvalho R F Schwan M N Pereira C L S Ávila Source Type: research
Diversity and probiotic characterisation of yeast isolates in the bovine gastrointestinal tract
The objective of this study was to isolate and identify yeasts present in the bovine feces and evaluate their probiotic potential together with strains previously isolated from the rumen (preliminary study). A total of 193 isolates were studied, including 139 isolates (19 species) from fecal samples from 11 different animals (Bos taurus and Bos indicus) and 54 strains previously isolated from rumen fluid (Bos taurus). The yeast population in the feces ranged from 3.51 to 4.99 log CFU/g, with Candida pararugosa being the most abundant (isolated from the feces of six samples analysed). Isolates were selected that had negativ...
Source: Antonie van Leeuwenhoek - August 31, 2023 Category: Microbiology Authors: G F C Sales B F Carvalho R F Schwan M N Pereira C L S Ávila Source Type: research
Antimicrobial resistance- and pathogen patterns in the fecal microbiota of sows and their offspring in German commercial pig farms
In this study, non-metric multidimensional scaling, hierarchical clustering, and l atent class analysis were used to determine the impact of antibiotic use on antibiotic resistance patterns and pathogen prevalence in 20 German pig farms. This may help to develop individualized health strategies. 802 fresh fecal samples were collected from sows and piglets from 20 piglet production and rearing farms at different production times (sows antepartum and postpartum, suckling piglets, weaned piglets). In addition, the use of antibiotics was recorded. DNA extracts were subjected to quantitative real-time qPCR with primers specific...
Source: PLoS One - August 24, 2023 Category: Biomedical Science Authors: Anja L ührmann Source Type: research
Prevalence of Clostridium perfringens toxinotypes in antibiotic-associated diarrhoeal (AAD) patients in Iranian hospitals; can toxinotype D serve as a possible zoonotic agent for humans?
This study persists in considering toxinotype F in Iranian AAD patients as it was the dominant C. perfringens toxinotype. Remarkably, the isolation of toxinotype D suggests it as a potential trigger in C. perfringens-associated AAD for the first time and highlights it as a possible zoonotic agent for humans.PMID:37597720 | DOI:10.1016/j.actatropica.2023.107002 (Source: Acta Tropica)
Source: Acta Tropica - August 19, 2023 Category: Infectious Diseases Authors: Mojtaba Alimolaei Sadegh Afzali Source Type: research
Prevalence of Clostridium perfringens toxinotypes in antibiotic-associated diarrhoeal (AAD) patients in Iranian hospitals; can toxinotype D serve as a possible zoonotic agent for humans?
This study persists in considering toxinotype F in Iranian AAD patients as it was the dominant C. perfringens toxinotype. Remarkably, the isolation of toxinotype D suggests it as a potential trigger in C. perfringens-associated AAD for the first time and highlights it as a possible zoonotic agent for humans.PMID:37597720 | DOI:10.1016/j.actatropica.2023.107002 (Source: Acta Tropica)
Source: Acta Tropica - August 19, 2023 Category: Infectious Diseases Authors: Mojtaba Alimolaei Sadegh Afzali Source Type: research
Prevalence of Clostridium perfringens toxinotypes in antibiotic-associated diarrhoeal (AAD) patients in Iranian hospitals; can toxinotype D serve as a possible zoonotic agent for humans?
This study persists in considering toxinotype F in Iranian AAD patients as it was the dominant C. perfringens toxinotype. Remarkably, the isolation of toxinotype D suggests it as a potential trigger in C. perfringens-associated AAD for the first time and highlights it as a possible zoonotic agent for humans.PMID:37597720 | DOI:10.1016/j.actatropica.2023.107002 (Source: Acta Tropica)
Source: Acta Tropica - August 19, 2023 Category: Infectious Diseases Authors: Mojtaba Alimolaei Sadegh Afzali Source Type: research
Prevalence of Clostridium perfringens toxinotypes in antibiotic-associated diarrhoeal (AAD) patients in Iranian hospitals; can toxinotype D serve as a possible zoonotic agent for humans?
This study persists in considering toxinotype F in Iranian AAD patients as it was the dominant C. perfringens toxinotype. Remarkably, the isolation of toxinotype D suggests it as a potential trigger in C. perfringens-associated AAD for the first time and highlights it as a possible zoonotic agent for humans.PMID:37597720 | DOI:10.1016/j.actatropica.2023.107002 (Source: Acta Tropica)
Source: Acta Tropica - August 19, 2023 Category: Infectious Diseases Authors: Mojtaba Alimolaei Sadegh Afzali Source Type: research
Clostridium perfringens Liver Abscess Disguised as Biliary Disease: A Report of Two Cases and a Review of the Literature
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - August 11, 2023 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
