Characterization and heterologous expression of plasmalogen synthase MeHAD from Megasphaera elsdenii
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jun 20:159358. doi: 10.1016/j.bbalip.2023.159358. Online ahead of print.ABSTRACTPlasmalogens (Pls) are vinyl-ether bond-containing glycerophospholipids or glycosyl diradyl glycerols, and are of great importance in the physiological functions and stability of cell membrane. Here, we identified and characterized that the plasmalogen synthase MeHAD from anaerobic Megasphaera elsdenii was responsible for vinyl-ether bond formation. Different from the 2-hydroxyacyl-CoA dehydratase (HAD) family plasmalogen synthase PlsA-PlsR which are encoded by two genes in Clostridium perfringens...
Source: Mol Biol Cell - June 22, 2023 Category: Molecular Biology Authors: Furong Zhang Zhaonan Yang Yulong Zhou Binqiang Wang Zheming Xie Ning Yu Jie Zhao Howard Goldfine Shang Dai Genlin Zhang Bing Tian Source Type: research
Deconstruction of a multi-strain Bacillus-based probiotic used for poultry: an in vitro assessment of its individual components against C. perfringens
Probiotics have been used in poultry production to improve the performance and health of chickens raised without antibiotics. The combination of different probiotic strains has been used with the hope of confe... (Source: BMC Research Notes)
Source: BMC Research Notes - June 22, 2023 Category: Research Authors: Vinicius Buiatte, Maria Schultheis and Alberto Gino Lorenzoni Tags: Research Note Source Type: research
Phagocyte extracellular traps formation contributes to host defense against Clostridium perfringens infection
Cytokine. 2023 Jun 18;169:156276. doi: 10.1016/j.cyto.2023.156276. Online ahead of print.ABSTRACTClostridium perfringens (C. perfringens) is an important Gram-positive anaerobic spore-forming pathogen that provokes life-threatening gas gangrene and acute enterotoxaemia, although it colonizes as a component of the symbiotic bacteria in humans and animals. However, the mechanisms by which C. perfringens is cleared from the host remains poorly understood, thereby impeding the development of novel strategies for control this infection. Here, we uncover a beneficial effect of extracellular traps (ETs) formation on bacterial kil...
Source: Cytokine - June 20, 2023 Category: Molecular Biology Authors: Yang Liu Jing Liang Jian-Wei Li Li-Hua Xing Fen-Xin Li Na Wang Yu-Jing Wu Yu-Ze Ma Zhao-Rui Xing Xue Jiang Xin-Yue Zhang Zheng-Xuan Lei Xiao Wang Shui-Xing Yu Source Type: research
Phagocyte extracellular traps formation contributes to host defense against Clostridium perfringens infection
Cytokine. 2023 Jun 18;169:156276. doi: 10.1016/j.cyto.2023.156276. Online ahead of print.ABSTRACTClostridium perfringens (C. perfringens) is an important Gram-positive anaerobic spore-forming pathogen that provokes life-threatening gas gangrene and acute enterotoxaemia, although it colonizes as a component of the symbiotic bacteria in humans and animals. However, the mechanisms by which C. perfringens is cleared from the host remains poorly understood, thereby impeding the development of novel strategies for control this infection. Here, we uncover a beneficial effect of extracellular traps (ETs) formation on bacterial kil...
Source: Cytokine - June 20, 2023 Category: Molecular Biology Authors: Yang Liu Jing Liang Jian-Wei Li Li-Hua Xing Fen-Xin Li Na Wang Yu-Jing Wu Yu-Ze Ma Zhao-Rui Xing Xue Jiang Xin-Yue Zhang Zheng-Xuan Lei Xiao Wang Shui-Xing Yu Source Type: research
Clostridium perfringens epsilon-toxin requires acid sphingomyelinase for cellular entry
CONCLUSIONS: Based on the present results, ASMase is required for efficient internalization of epsilon-toxin.PMID:37308057 | DOI:10.1016/j.anaerobe.2023.102753 (Source: Anaerobe)
Source: Anaerobe - June 12, 2023 Category: Microbiology Authors: Yoshihiko Sakaguchi Keiko Kobayashi Masaya Takehara Masahiro Nagahama Source Type: research
