Lactic Acid Bacteria isolated from traditional and innovative alheiras as potential biocontrol agents
Food Microbiol. 2024 May;119:104450. doi: 10.1016/j.fm.2023.104450. Epub 2023 Dec 13.ABSTRACTFrom a selection of seven traditional and 14 innovative alheiras, 491 lactic acid bacteria (LAB) were isolated and tested for their antimicrobial activity against several food-borne pathogens. Among these, six strains revealed antimicrobial activity through potential bacteriocin production against 14 Listeria monocytogenes strains, Enterococcus faecalis ATCC 29212, Clostridium sporogenes ESB050, and Clostridium perfringens ESB054. Through whole genome sequencing (WGS), these strains were identified as Lactiplantibacillus plantarum ...
Source: Food Microbiology - January 15, 2024 Category: Food Science Authors: In ês Azevedo Joana Barbosa Helena Albano Teresa Nogueira Paula Teixeira Source Type: research
Evaluation of Natural Antimicrobial Substances Blend as a Replacement for Antibiotic Growth Promoters in Broiler Chickens: Enhancing Growth and Managing Intestinal Bacterial Diseases
Curr Microbiol. 2024 Jan 8;81(2):55. doi: 10.1007/s00284-023-03573-w.ABSTRACTIn recent years, commercial use of antibiotic growth promoter (AGP) has restrictions due to drug resistance against intestinal pathogenic bacteria: Escherichia coli, Salmonella, and Clostridium perfringens. Currently there is no single non-antibiotic treatment approach that is effective against intestinal illnesses in broiler chicken. Hence, present study aimed to analyze efficacy of blend of natural antimicrobial substances (probiotics, prebiotics, organic acids, and essential oils blend named as AGPR) as replacers of AGPs (BMD and CTC) for promo...
Source: Current Microbiology - January 8, 2024 Category: Microbiology Authors: Duraisamy Thirumeignanam Mahalingam Chellapandian Natarajan Arulnathan Sivamurthy Parthiban Veluchamy Kumar M Panchatcharam Vijayakumar Sunil Chauhan Source Type: research
Novel toxin-based mRNA vaccine against Clostridium perfringens using in silico approaches
In this study, computational tools were utilized to design a RNA vaccine targeting C. perfringens toxins. Toxin protein sequences were retrieved and their linear B-cell, MHCI, and MHCII binding epitopes were predicted. Allergenicity, toxigenicity, and IFN-γ induction were assessed to select non-allergenic, non-toxic, and IFN-γ-inducing epitopes. Molecular docking was performed to identify epitopes that fit within the binding cleft of MHC alleles. A final peptide vaccine construct was designed with selected epitopes separated by a linker sequence. The antigenicity and physicochemical properties of the vaccine were evaluat...
Source: Toxicon - January 7, 2024 Category: Toxicology Authors: Parisa Asadollahi Behrooz Sadeghi Kalani Source Type: research
Novel toxin-based mRNA vaccine against Clostridium perfringens using in silico approaches
In this study, computational tools were utilized to design a RNA vaccine targeting C. perfringens toxins. Toxin protein sequences were retrieved and their linear B-cell, MHCI, and MHCII binding epitopes were predicted. Allergenicity, toxigenicity, and IFN-γ induction were assessed to select non-allergenic, non-toxic, and IFN-γ-inducing epitopes. Molecular docking was performed to identify epitopes that fit within the binding cleft of MHC alleles. A final peptide vaccine construct was designed with selected epitopes separated by a linker sequence. The antigenicity and physicochemical properties of the vaccine were evaluat...
Source: Toxicon - January 7, 2024 Category: Toxicology Authors: Parisa Asadollahi Behrooz Sadeghi Kalani Source Type: research
IJERPH, Vol. 21, Pages 64: Characteristics of Gastroenteritis Outbreaks Investigated in Singapore: 2018 & ndash;2021
This study analyzed the annual trend of investigated gastroenteritis outbreaks, the proportion of outbreaks by implicated sources of food, and the proportion of the type of pathogens identified from human cases, food samples, and environmental swabs collected from outbreak investigations. Among the foodborne gastroenteritis outbreaks (n = 121) investigated in Singapore, approximately 42.1% of the outbreaks had food prepared by caterers, 14.9% by restaurants, and 12.4% had food prepared by in-house kitchens. Clostridium perfringens and Salmonella were the most common causative pathogens in foodborne outbreaks throughout the...
Source: International Journal of Environmental Research and Public Health - January 6, 2024 Category: Environmental Health Authors: Muhd Tarmidzi Fua ’di Benjamin Er Sylvester Lee Pei Pei Chan Joanna Khoo Desmond Tan Huilin Li Imran Roshan Muhammad Pream Raj Lalitha Kurupatham Vernon Lee Li Kiang Tan Joanne Sheot Harn Chan Angela Li Kyaw Thu Aung Tags: Article Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins
CONCLUSIONS: C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.PMID:38163631 | DOI:10.1016/j.anaerobe.2023.102817 (Source: Anaerobe)
Source: Anaerobe - January 1, 2024 Category: Microbiology Authors: Guilherme Guerra Alves Ronnie Antunes Assis Victor Santos do Amarante Carlos Augusto de Oliveira J únior Rodrigo Ot ávio Silveira Silva Luiz Guilherme Dias Heneine Francisco Carlos Faria Lobato Source Type: research
The evaluations of the inhibition of orlistat on Clostridium perfringens sialidase (NanI) activity by in vitro and in silico approaches
Chem Biodivers. 2023 Dec 29:e202301634. doi: 10.1002/cbdv.202301634. Online ahead of print.ABSTRACTClostridium perfringens (C. perfringens) is a bacterium that causes serious problems in humans and animals such as food poisoning, gas gangrene and infections. C. perfringens has three sialidases (NanH, NanI, NanJ) and inhibition of NanI constitutes an approach in the treatment of C. perfringens since NanI provides the carbohydrate source necessary for the growth of bacteria. In our study, the inhibition effect of some drugs belonging to different drug groups on NanI activity was investigated. Among these drugs, orlistat (0.2...
Source: Chemistry and Biodiversity - December 29, 2023 Category: Biochemistry Authors: Onur Ertik Refiye Yanardag Source Type: research
The evaluations of the inhibition of orlistat on Clostridium perfringens sialidase (NanI) activity by in vitro and in silico approaches
Chem Biodivers. 2023 Dec 29:e202301634. doi: 10.1002/cbdv.202301634. Online ahead of print.ABSTRACTClostridium perfringens (C. perfringens) is a bacterium that causes serious problems in humans and animals such as food poisoning, gas gangrene and infections. C. perfringens has three sialidases (NanH, NanI, NanJ) and inhibition of NanI constitutes an approach in the treatment of C. perfringens since NanI provides the carbohydrate source necessary for the growth of bacteria. In our study, the inhibition effect of some drugs belonging to different drug groups on NanI activity was investigated. Among these drugs, orlistat (0.2...
Source: Chemistry and Biodiversity - December 29, 2023 Category: Biochemistry Authors: Onur Ertik Refiye Yanardag Source Type: research
The evaluations of the inhibition of orlistat on Clostridium perfringens sialidase (NanI) activity by in vitro and in silico approaches
Chem Biodivers. 2023 Dec 29:e202301634. doi: 10.1002/cbdv.202301634. Online ahead of print.ABSTRACTClostridium perfringens (C. perfringens) is a bacterium that causes serious problems in humans and animals such as food poisoning, gas gangrene and infections. C. perfringens has three sialidases (NanH, NanI, NanJ) and inhibition of NanI constitutes an approach in the treatment of C. perfringens since NanI provides the carbohydrate source necessary for the growth of bacteria. In our study, the inhibition effect of some drugs belonging to different drug groups on NanI activity was investigated. Among these drugs, orlistat (0.2...
Source: Chemistry and Biodiversity - December 29, 2023 Category: Biochemistry Authors: Onur Ertik Refiye Yanardag Source Type: research
