Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms
In conclusion, raw milk could serve as a medium for the transmission of C. perfringens, which could result in human food poisoning. Monitoring and controlling several points of cross-contamination during the milking process are essential as is implementing stringent hygiene measures to prevent further spread and reduce the risk of C. perfringens infection.PMID:38431330 | DOI:10.1016/j.fm.2024.104485 (Source: Food Microbiology)
Source: Food Microbiology - March 2, 2024 Category: Food Science Authors: Yanfen Jiang Yifan Pan Jingyi Yin Source Type: research
Toxin gene detection and antibiotic resistance of Clostridium perfringens from aquatic sources
Int J Food Microbiol. 2024 Feb 28;415:110642. doi: 10.1016/j.ijfoodmicro.2024.110642. Online ahead of print.ABSTRACTClostridium perfringens is a zoonotic opportunistic pathogen that produces toxins that can cause necrotic enteritis and even "sudden death disease". This bacterium is widely distributed in the intestines of livestock and human, but there are few reports of distribution in aquatic animals (Hafeez et al., 2022). In order to explore the isolation rate of C. perfringens and the toxin genes they carry, 141 aquatic samples, including clams (Ruditapes philippinarum), oysters (Ostreidae), and mud snails (Bullacta exe...
Source: International Journal of Food Microbiology - March 1, 2024 Category: Food Science Authors: Mengxuan Li Ying Wang Bingyu Hou Yibao Chen Ming Hu Xiaonan Zhao Qing Zhang Lulu Li Yanbo Luo Yuqing Liu Yumei Cai Source Type: research
Toxin gene detection and antibiotic resistance of Clostridium perfringens from aquatic sources
Int J Food Microbiol. 2024 Feb 28;415:110642. doi: 10.1016/j.ijfoodmicro.2024.110642. Online ahead of print.ABSTRACTClostridium perfringens is a zoonotic opportunistic pathogen that produces toxins that can cause necrotic enteritis and even "sudden death disease". This bacterium is widely distributed in the intestines of livestock and human, but there are few reports of distribution in aquatic animals (Hafeez et al., 2022). In order to explore the isolation rate of C. perfringens and the toxin genes they carry, 141 aquatic samples, including clams (Ruditapes philippinarum), oysters (Ostreidae), and mud snails (Bullacta exe...
Source: International Journal of Food Microbiology - March 1, 2024 Category: Food Science Authors: Mengxuan Li Ying Wang Bingyu Hou Yibao Chen Ming Hu Xiaonan Zhao Qing Zhang Lulu Li Yanbo Luo Yuqing Liu Yumei Cai Source Type: research
Natural intraepithelial lymphocyte populations rise during necrotic enteritis in chickens
Intraepithelial lymphocytes (IEL) reside in the epithelium at the interface between the contents of the intestinal lumen and the sterile environment of the lamina propria. Because of this strategic location, IEL play a crucial role in various immunological processes, ranging from pathogen control to tissue stability. In mice and humans, IEL exhibit high diversity, categorized into induced IEL (conventional CD4 and CD8αβ T cells) and natural IEL (TCRαβCD8αα, TCRγδ, and TCRneg IEL). In chickens, however, the subpopulations of IEL and their functions in enteric diseases remain unclear. Thus, we conducted this study to...
Source: Frontiers in Immunology - February 22, 2024 Category: Allergy & Immunology Source Type: research
< em > Clostridium perfringens < /em > sialidase interaction with Neu5Ac α-Gal sialic acid receptors by < em > in-silico < /em > observation and its impact on monolayers cellular behavior structure
CONCLUSION: Sialidase-derived C. perfringens has the capacity to compete with viral molecules for attachment to host sialic acid based on in silico analysis. However, sialidase treatment has an impact on monolayer cell fibroblasts given exposure to high doses.PMID:38370892 | PMC:PMC10868698 | DOI:10.5455/javar.2023.j722 (Source: Cell Research)
Source: Cell Research - February 19, 2024 Category: Cytology Authors: Ryan Septa Kurnia Amin Soebandrio Vivi Hardianty Harun Christian Marco Hadi Nugroho Desak Gede Budi Krisnamurti Okti Nadia Poetri Agustin Indrawati Simson Tarigan Ketut Karuni Nyanakumari Natih Fera Ibrahim Pratiwi Pudjilestari Sudarmono Otto Sahat Martua Source Type: research
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge....
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Y Goa J G Du C Jirapattharasate E Galon S W Ji Z G Ran Y Q Xia Source Type: research
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge....
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Y Goa J G Du C Jirapattharasate E Galon S W Ji Z G Ran Y Q Xia Source Type: research
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge....
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Y Goa J G Du C Jirapattharasate E Galon S W Ji Z G Ran Y Q Xia Source Type: research
A non-toxic recombinant protein rSUMO-CPBm4 as a potential vaccine candidate against Clostridium perfringens type C beta enterotoxemia
In this study, we synthesized the rCPBm4 of C. perfringens type C strain and small ubiquitin-like modifier (SUMO)-tag CPBm4 (rSUMO-CPBm4) by introducing four amino acid substitutions: R212E, Y266A, L268G, and W275A. Compared with rCPBm4, rSUMO-CPBm4 was expressed with higher solubility in Escherichia coli BL21 (DE3). Neither rCPBm4 nor rSUMO-CPBm4 was lethal to mice. Although rCPBm4 and rSUMO-CPBm4 were reactogenic with polyclonal antibodies against crude CPB, rabbits vaccinated with rSUMO-CPBm4 developed significant levels of toxin-neutralizing antibody (TNA) titers that conferred protection against crude toxin challenge....
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Y Goa J G Du C Jirapattharasate E Galon S W Ji Z G Ran Y Q Xia Source Type: research
