Re-discovery of MS-347a as a fungicide candidate through a new drug discovery platform with a multidrug-sensitive Saccharomyces cerevisiae screening system and the introduction of a global secondary metabolism regulator, laeA gene
Biosci Biotechnol Biochem. 2024 Apr 25:zbae050. doi: 10.1093/bbb/zbae050. Online ahead of print.ABSTRACTWe found that the culture broth of fungi showed anti-fungal activity against multidrug-sensitive budding yeast. However, we could not identify the anti-fungal compound due to the small quantity. Therefore, we attempted to increase the productivity of the target compound by the introduction of a global secondary metabolism regulator, laeA to the strain, which led to the successful isolation of ten-folds greater amount of MS-347a (1) than Aspergillus sp. FKI-5362. Compound 1 was not effective against Candida albicans and t...
Source: Bioscience, Biotechnology, and Biochemistry - April 25, 2024 Category: Biochemistry Authors: Sota Honma Aoi Kimishima Atsushi Kimishima Masako Honsho Hiroki Kojima Toshiyuki Tokiwa Atsuka Nishitomi Satoshi Kato Naozumi Kondo Yasuko Araki Tadashi Takahashi Takumi Chinen Takeo Usui Shin-Ichi Fuji Kotaro Ito Yukihiro Asami Source Type: research
Fungal biomineralization of toxic metals accelerates organic pollutant removal
Curr Biol. 2024 Apr 16:S0960-9822(24)00444-5. doi: 10.1016/j.cub.2024.04.005. Online ahead of print.ABSTRACTFungal biomineralization plays an important role in the biogeochemical cycling of metals in the environment and has been extensively explored for bioremediation and element biorecovery. However, the cellular and metabolic responses of fungi in the presence of toxic metals during biomineralization and their impact on organic matter transformations are unclear. This is an important question because co-contamination by toxic metals and organic pollutants is a common phenomenon in the natural environment. In this researc...
Source: Current Biology - April 25, 2024 Category: Biology Authors: Qianwei Li Miao Zhang Biao Wei Wei Lan Qinghong Wang Chunmao Chen Huazhang Zhao Daoqing Liu Geoffrey Michael Gadd Source Type: research
Enzymatic Performance of < em > Aspergillus oryzae < /em > α-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies
In this study, the enzymatic performance of Aspergillus oryzae α-amylase (Taka-amylase) in various organic solvents both experimentally and computationally was investigated. The results demonstrated that ethanol and ether sustain Taka-amylase activity up to 20% to 25% of the organic solvents, with ether providing twice the stability of ethanol. Molecular dynamics simulations further revealed that Taka-amylase has a more stable structure in ether and ethanol relative to other organic solvents. In addition, the analysis showed that the loop located near the active site in the AB-domain is a vulnerable site for enzyme destab...
Source: Bioinformatics and Biology Insights - April 25, 2024 Category: Bioinformatics Authors: Khomaini Hasan Umi Baroroh Indri Novia Madhani Zahra Silmi Muscifa Mia Tria Novianti Muhamad Abidin Muhammad Yusuf Toto Subroto Source Type: research
Re-discovery of MS-347a as a fungicide candidate through a new drug discovery platform with a multidrug-sensitive Saccharomyces cerevisiae screening system and the introduction of a global secondary metabolism regulator, laeA gene
Biosci Biotechnol Biochem. 2024 Apr 25:zbae050. doi: 10.1093/bbb/zbae050. Online ahead of print.ABSTRACTWe found that the culture broth of fungi showed anti-fungal activity against multidrug-sensitive budding yeast. However, we could not identify the anti-fungal compound due to the small quantity. Therefore, we attempted to increase the productivity of the target compound by the introduction of a global secondary metabolism regulator, laeA to the strain, which led to the successful isolation of ten-folds greater amount of MS-347a (1) than Aspergillus sp. FKI-5362. Compound 1 was not effective against Candida albicans and t...
Source: Bioscience, Biotechnology, and Biochemistry - April 25, 2024 Category: Biochemistry Authors: Sota Honma Aoi Kimishima Atsushi Kimishima Masako Honsho Hiroki Kojima Toshiyuki Tokiwa Atsuka Nishitomi Satoshi Kato Naozumi Kondo Yasuko Araki Tadashi Takahashi Takumi Chinen Takeo Usui Shin-Ichi Fuji Kotaro Ito Yukihiro Asami Source Type: research
Enzymatic Performance of < em > Aspergillus oryzae < /em > α-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies
In this study, the enzymatic performance of Aspergillus oryzae α-amylase (Taka-amylase) in various organic solvents both experimentally and computationally was investigated. The results demonstrated that ethanol and ether sustain Taka-amylase activity up to 20% to 25% of the organic solvents, with ether providing twice the stability of ethanol. Molecular dynamics simulations further revealed that Taka-amylase has a more stable structure in ether and ethanol relative to other organic solvents. In addition, the analysis showed that the loop located near the active site in the AB-domain is a vulnerable site for enzyme destab...
Source: Bioinformatics and Biology Insights - April 25, 2024 Category: Bioinformatics Authors: Khomaini Hasan Umi Baroroh Indri Novia Madhani Zahra Silmi Muscifa Mia Tria Novianti Muhamad Abidin Muhammad Yusuf Toto Subroto Source Type: research
Enzymatic Performance of < em > Aspergillus oryzae < /em > α-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies
In this study, the enzymatic performance of Aspergillus oryzae α-amylase (Taka-amylase) in various organic solvents both experimentally and computationally was investigated. The results demonstrated that ethanol and ether sustain Taka-amylase activity up to 20% to 25% of the organic solvents, with ether providing twice the stability of ethanol. Molecular dynamics simulations further revealed that Taka-amylase has a more stable structure in ether and ethanol relative to other organic solvents. In addition, the analysis showed that the loop located near the active site in the AB-domain is a vulnerable site for enzyme destab...
Source: Bioinformatics and Biology Insights - April 25, 2024 Category: Bioinformatics Authors: Khomaini Hasan Umi Baroroh Indri Novia Madhani Zahra Silmi Muscifa Mia Tria Novianti Muhamad Abidin Muhammad Yusuf Toto Subroto Source Type: research
Real-life experience on the effectiveness of conjunctival flap and amniotic membrane graft in the treatment of refractory fungal corneal ulcer
ConclusionsCF and AMG are both effective for the management of refractory fungal corneal ulcers. However, AMG appears to improve visual acuity better than CF. (Source: Japanese Journal of Ophthalmology)
Source: Japanese Journal of Ophthalmology - April 25, 2024 Category: Opthalmology Source Type: research
Garden-variety fungus is an expert at environmental clean-ups
Nature, Published online: 25 April 2024; doi:10.1038/d41586-024-01145-xThe common fungus Aspergillus niger removes both heavy metals and organic pollutants from its surroundings. (Source: Nature AOP)
Source: Nature AOP - April 25, 2024 Category: Research Source Type: research
Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique
Kojic acid is a wonderful fungal secondary metabolite that has several applications in the food, medical, and agriculture sectors. Many human diseases become resistant to normal antibiotics and normal treatmen... (Source: BMC Microbiology)
Source: BMC Microbiology - April 25, 2024 Category: Microbiology Authors: Ghada Abd-Elmonsef Mahmoud, Abo bakr Abdel Shakor, Nahla A. Kamal-Eldin and Abdel-Naser A. Zohri Tags: Research Source Type: research
Antifungal and Antivirulence Activity of Vanillin and Tannic Acid Against Aspergillus fumigatus and Fusarium solani
In this study, we evaluated the antifungal and antivirulence effects of vanillin and tannic acid against Aspergillus fumigatus and Fusarium solani. The minimum inhibitory concentrations of the compounds were determined by the microdilution method in RPMI broth in 96-well microplates according to CLSI. Conidial germination, protease production, biofilm formation, and in vivo therapeutic efficacy assays were performed. The results demonstrated that vanillin and tannic acid had antifungal activity against Aspergillus fumigatus, while tannic acid only exhibited antifungal activity against Fusarium solani. We found that vanilli...
Source: Current Microbiology - April 24, 2024 Category: Microbiology Authors: Ana L R íos-López Jorge D ávila-Aviña Gloria M Gonz ález Orlando Flores-Maldonado Source Type: research
Assessment of Portuguese fitness centers: bridging the knowledge gap on harmful microbial contamination with focus on fungi
This study intends to characterize microbial contamination in Portuguese FC by passive sampling methods: electrostatic dust collectors (EDC) (N=39), settled dust (N=8), vacuum filters (N=8), and used cleaning mops (N=12). The obtained extracts were plated in selective culture media for fungi and bacteria. Filters, EDC, and mop samples' extracts were also screened for antifungal resistance and used for the molecular detection of the selected Aspergillus sections. The detection of mycotoxins was conducted using a high-performance liquid chromatograph (HPLC) system and to determine the cytotoxicity of microbial contaminants r...
Source: Environmental Pollution - April 24, 2024 Category: Environmental Health Authors: Carla Viegas C átia Peixoto Bianca Gomes Marta Dias Renata Cervantes Pedro Pena Klara Slezakova Maria do Carmo Pereira Simone Morais Elisabete Carolino Magdalena Twaru żek Susana Viegas Liliana Aranha Caetano Source Type: research
Synergistic antimicrobial action of nanocellulose, nanoselenium, and nanocomposite against pathogenic microorganisms
In this study, nanocellulose was extracted from algae, biomolecule substances synthesized selenium nanoparticles, and a simple nanocomposite of nanocellulose and nanoselenium was elaborated using nanocellulose as a reducing agent under hydrothermal conditions. These nanocomposite materials have markedly improved properties at low concentrations. Our obtained polymers were characterized using techniques including Fourier-transform infrared spectroscopy, X-ray powder diffraction, Thermo gravimetric analysis (TGA), Scanning electron microscopic (SEM), Energy Dispersive X-ray analysis (EDX), Transmission electron microscopic (...
Source: International Journal of Biological Macromolecules - April 24, 2024 Category: Biochemistry Authors: Mostafa M El-Sheekh Wesam E Yousuf Tarek M Mohamed El-Refaie Kenawy Source Type: research
The pleiotropic phenotype of FlbA of Aspergillus niger is explained in part by the activity of seven of its downstream-regulated transcription factors
Fungal Genet Biol. 2024 Apr 23;172:103894. doi: 10.1016/j.fgb.2024.103894. Online ahead of print.ABSTRACTInactivation of flbA in Aspergillus niger results in thinner cell walls, increased cell lysis, abolished sporulation, and an increased secretome complexity. A total of 36 transcription factor (TF) genes are differentially expressed in ΔflbA. Here, seven of these genes (abaA, aslA, aslB, azf1, htfA, nosA, and srbA) were inactivated. Inactivation of each of these genes affected sporulation and, with the exception of abaA, cell wall integrity and protein secretion. The impact on secretion was strongest in the case of Δas...
Source: Fungal Genetics and Biology - April 24, 2024 Category: Biology Authors: Xiaoyi Chen Juan P Moran Torres Peter Jan Vonk J Mirjam A Damen Karli R Reiding Jan Dijksterhuis Luis G Lugones Han A B W östen Source Type: research
Antifungal and Antivirulence Activity of Vanillin and Tannic Acid Against Aspergillus fumigatus and Fusarium solani
In this study, we evaluated the antifungal and antivirulence effects of vanillin and tannic acid against Aspergillus fumigatus and Fusarium solani. The minimum inhibitory concentrations of the compounds were determined by the microdilution method in RPMI broth in 96-well microplates according to CLSI. Conidial germination, protease production, biofilm formation, and in vivo therapeutic efficacy assays were performed. The results demonstrated that vanillin and tannic acid had antifungal activity against Aspergillus fumigatus, while tannic acid only exhibited antifungal activity against Fusarium solani. We found that vanilli...
Source: Current Microbiology - April 24, 2024 Category: Microbiology Authors: Ana L R íos-López Jorge D ávila-Aviña Gloria M Gonz ález Orlando Flores-Maldonado Source Type: research
Assessment of Portuguese fitness centers: bridging the knowledge gap on harmful microbial contamination with focus on fungi
This study intends to characterize microbial contamination in Portuguese FC by passive sampling methods: electrostatic dust collectors (EDC) (N=39), settled dust (N=8), vacuum filters (N=8), and used cleaning mops (N=12). The obtained extracts were plated in selective culture media for fungi and bacteria. Filters, EDC, and mop samples' extracts were also screened for antifungal resistance and used for the molecular detection of the selected Aspergillus sections. The detection of mycotoxins was conducted using a high-performance liquid chromatograph (HPLC) system and to determine the cytotoxicity of microbial contaminants r...
Source: Environmental Pollution - April 24, 2024 Category: Environmental Health Authors: Carla Viegas C átia Peixoto Bianca Gomes Marta Dias Renata Cervantes Pedro Pena Klara Slezakova Maria do Carmo Pereira Simone Morais Elisabete Carolino Magdalena Twaru żek Susana Viegas Liliana Aranha Caetano Source Type: research
