A catalytic membrane approach as a way to obtain sweet and unsweet lactose-free milk
Bioprocess Biosyst Eng. 2024 Apr 22. doi: 10.1007/s00449-024-03018-z. Online ahead of print.ABSTRACTThe growing need in the current market for innovative solutions to obtain lactose-free (L-F) milk is caused by the annual increase in the prevalence of lactose intolerance inside as well as the newborn, children, and adults. Various configurations of enzymes can yield two distinct L-F products: sweet (β-galactosidase) and unsweet (β-galactosidase and glucose oxidase) L-F milk. In addition, the reduction of sweetness through glucose decomposition should be performed in a one-pot mode with catalase to eliminate product inhib...
Source: Bioprocess and Biosystems Engineering - April 21, 2024 Category: Biomedical Engineering Authors: Katarzyna Czy żewska Anna Trusek Source Type: research
Antifungal efficiency and mechanisms of ethyl ferulate against postharvest pathogens
This study evaluated the antifungal activity of ethyl ferulate (EF) and explored its mechanisms of action against Alternaria alternata, Aspergillus niger, Botrytis cinerea, Penicillium expansum, Penicillium digitatum, Geotrichum candidum and evaluated its potential to inhibit postharvest decay. The results demonstrated that EF exerts potent antifungal activity against a wide board of postharvest pathogens. Results also revealed that its antifungal mechanism is multifaceted: EF may be involved in binding to and disturbing the integrity of the fungal plasma membrane, causing leakage of intracellular content and losing normal...
Source: International Journal of Food Microbiology - April 21, 2024 Category: Food Science Authors: Chang Shu Xiuxiu Sun Jiankang Cao Samir Droby Weibo Jiang Source Type: research
Growth-promoting effects of Aspergillus Elegans and the dark septate endophyte (DSE) Periconia macrospinosa on cucumber
In this study, two endophytes isolated from wild plant roots, were tested to determine their effect on the growth promotion of cucumber (Cucumis sativus L.) plants. The phylogenetic analysis revealed that the designated isolates were Aspergillus elegans and Periconia macrospinosa. The results of the Plant Growth Promoting Fungal (PGPF) tests showed that both Aspergillus elegans and Periconia macrospinosa have a zinc solubilizing capacity, especially A. elegans, with a solubilization index higher than 80%. Also, both have a high salt tolerance (10-15% NaCl for P. macrospinosa and A. elegans, respectively), cellulolytic acti...
Source: Archives of Microbiology - April 20, 2024 Category: Microbiology Authors: Warda Sidhoum Soulef Dib Yousra Alim Sarra Anseur Sabrina Benlatreche Zoulikha Mouna Belaidouni Fatiha El Zahra Chamouma Source Type: research
Characterization of Bacillus amyloliquefaciens PM415 as a potential bio-preserving probiotic
Arch Microbiol. 2024 Apr 20;206(5):222. doi: 10.1007/s00203-024-03953-1.ABSTRACTAnimal feed is vulnerable to fungal infections, and the use of bio-preserving probiotics has received increasing attention. In contrast to Lactobacillus and Bifidobacteria spp., fewer Bacillus spp. have been recognized as antifungal probiotics. Therefore, our objective was to screen antifungal strains and provide more Bacillus candidates to bridge this gap. Here, we screened 56 bacterial strains for cyclic lipopeptide genes and conducted an antifungal assay with Aspergillus niger as a representative fungus. We found that a Bacillus strain Bacil...
Source: Archives of Microbiology - April 20, 2024 Category: Microbiology Authors: Jingkang Ye Haiyang Wu Li Feng Qinghua Huang Qingxin Li Weiming Liao Jin Chuan Wu Source Type: research
Bioactive volatiles of brewer's yeasts: Antifungal action of compounds produced during wort fermentation on Aspergillus sp
Int J Food Microbiol. 2024 Apr 4;417:110692. doi: 10.1016/j.ijfoodmicro.2024.110692. Online ahead of print.ABSTRACTPrevious investigations proved the potential of Saccharomyces cerevisiae MBELGA62 and Pichia kudriavzevii MBELGA61 as suitable biocontrolling agents against Aspergillus sp. through the production of soluble and volatile bioactive antifungal compounds. The present study delves into those finding by means of the identification of the volatile compounds produced by brewer's strains that demonstrated fungistatic and fungicidal effects against Aspergillus flavus and A. parasiticus when cultured in brewer's wort aga...
Source: International Journal of Food Microbiology - April 19, 2024 Category: Food Science Authors: Sof ía Sampaolesi Laura P érez-Través Laura E Briand Amparo Querol Source Type: research
Alternative splicing analysis of lignocellulose-degrading enzyme genes and enzyme variants in Aspergillus niger
Appl Microbiol Biotechnol. 2024 Apr 19;108(1):302. doi: 10.1007/s00253-024-13137-y.ABSTRACTAlternative splicing (AS) greatly expands the protein diversity in eukaryotes. Although AS variants have been frequently reported existing in filamentous fungi, it remains unclear whether lignocellulose-degrading enzyme genes in industrially important fungi undergo AS events. In this work, AS events of lignocellulose-degrading enzymes genes in Aspergillus niger under two carbon sources (glucose and wheat straw) were investigated by RNA-Seq. The results showed that a total of 23 out of the 56 lignocellulose-degrading enzyme genes had ...
Source: Applied Microbiology and Biotechnology - April 19, 2024 Category: Microbiology Authors: Yifan Xu Feiyu Dong Ruoxin Wang Maria Ajmal Xinyu Liu Hui Lin Hongge Chen Source Type: research
Discovery of ( ±)-Penindolenes Reveals an Unusual Indole Ring Cleavage Pathway Catalyzed by P450 Monooxygenase
Angew Chem Int Ed Engl. 2024 Apr 18:e202403963. doi: 10.1002/anie.202403963. Online ahead of print.ABSTRACT(±)-Penindolenes A-D (1-4), the first representatives of indole terpenoids featuring a γ-lactam skeleton, were isolated from the mangrove-derived endophytic fungus Penicillium brocae MA-231. Our bioactivity tests revealed their potent antimicrobial and acetylcholinesterase inhibitory activities. The biosynthetic reactions by the five enzymes PbaABCDE leading to γ-lactam ring formation were identified with heterologous expression and in vitro enzymatic assays. Remarkably, the cytochrome P450 monooxygenase PbaB and i...
Source: Angewandte Chemie - April 18, 2024 Category: Chemistry Authors: Ling-Hong Meng Takayoshi Awakawa Xiao-Ming Li Zhiyang Quan Sui-Qun Yang Bin-Gui Wang Ikuro Abe Source Type: research
Malolactic fermentation in lingonberry juice and its use as a preservative
This study aimed to improve the taste of lingonberry juice by subjecting it to malolactic fermentation in order to reduce the sourness, and to investigate the benzoic acid in lingonberries as a natural preservative in juice blends by determining the microbial stability. After initial screening of lactic acid bacteria, a Lactiplantibacillus plantarum strain was used as the starter for subsequent investigations. Upon raising the pH, all malic acid was completely converted to lactic acid after seven days. The fermented juice was mixed with blackcurrant juice in different proportions. Challenge tests of the blends showed Liste...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Martina K Bergentall Jun Niimi Ingela Persson Emeline Calmet Dorine As Alexander Plovie Loredana Malafronte Petter Melin Source Type: research
Comparing resistance of bacterial spores and fungal conidia to pulsed light and UVC radiation at a wavelength of 254 nm
Food Microbiol. 2024 Aug;121:104518. doi: 10.1016/j.fm.2024.104518. Epub 2024 Mar 20.ABSTRACTPulsed light (PL) inactivates microorganisms by UV-rich, high-irradiance and short time pulses (250 μs) of white light with wavelengths from 200 nm to 1100 nm. PL is applied for disinfection of food packaging material and food-contact equipment. Spores of seven Bacillus ssp. strains and one Geobacillus stearothermophilus strain and conidia of filamentous fungi (One strain of Aspergillus brasiliensis, A. carbonarius and Penicillium rubens) were submitted to PL (fluence from 0.23 J/cm2 to 4.0 J/cm2) and UVC (at λ = 254 nm; fluence ...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Imed Dorbani Alain Berberian Christophe Riedel Catherine Duport Fr édéric Carlin Source Type: research
Synergistic antifungal mechanism of cinnamaldehyde and nonanal against Aspergillus flavus and its application in food preservation
Food Microbiol. 2024 Aug;121:104524. doi: 10.1016/j.fm.2024.104524. Epub 2024 Mar 25.ABSTRACTAspergillus flavus colonization on agricultural products during preharvest and postharvest results in tremendous economic losses. Inspired by the synergistic antifungal effects of essential oils, the aims of this study were to explore the mechanism of combined cinnamaldehyde and nonanal (SCAN) against A. flavus and to evaluate the antifungal activity of SCAN loading into diatomite (DM). Shriveled mycelia were observed by scanning electron microscopy, especially in the SCAN treatment group. Calcofluor white staining, transmission el...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Wei Zhang Cuixiang Li Yangyong Lv Shan Wei Yuansen Hu Source Type: research
Fungicidal efficiency of DBD cold plasma against Aspergillus niger on dried jujube
This study investigated the fungicidal efficiency and mechanism of action of dielectric barrier discharge cold atmosphere plasma (DBD-CAP) in inactivating Aspergillus niger (A. niger) spores. The disinfection efficacy and quality of dried jujube used as the processing application object were also studied. The results indicated that the Weibull + Tail model performed better for spore inactivation curves at different voltages among various treatment times, and the spore cells were reduced by 4.05 log (cfu/mL) in spores suspension at 70 kV after 15 min of treatment. This disinfection impact was further supported by scanning e...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Qiaoyun Wei Yuan Yuan Jianhao Zhang Jin Wang Source Type: research
Malolactic fermentation in lingonberry juice and its use as a preservative
This study aimed to improve the taste of lingonberry juice by subjecting it to malolactic fermentation in order to reduce the sourness, and to investigate the benzoic acid in lingonberries as a natural preservative in juice blends by determining the microbial stability. After initial screening of lactic acid bacteria, a Lactiplantibacillus plantarum strain was used as the starter for subsequent investigations. Upon raising the pH, all malic acid was completely converted to lactic acid after seven days. The fermented juice was mixed with blackcurrant juice in different proportions. Challenge tests of the blends showed Liste...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Martina K Bergentall Jun Niimi Ingela Persson Emeline Calmet Dorine As Alexander Plovie Loredana Malafronte Petter Melin Source Type: research
Comparing resistance of bacterial spores and fungal conidia to pulsed light and UVC radiation at a wavelength of 254 nm
Food Microbiol. 2024 Aug;121:104518. doi: 10.1016/j.fm.2024.104518. Epub 2024 Mar 20.ABSTRACTPulsed light (PL) inactivates microorganisms by UV-rich, high-irradiance and short time pulses (250 μs) of white light with wavelengths from 200 nm to 1100 nm. PL is applied for disinfection of food packaging material and food-contact equipment. Spores of seven Bacillus ssp. strains and one Geobacillus stearothermophilus strain and conidia of filamentous fungi (One strain of Aspergillus brasiliensis, A. carbonarius and Penicillium rubens) were submitted to PL (fluence from 0.23 J/cm2 to 4.0 J/cm2) and UVC (at λ = 254 nm; fluence ...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Imed Dorbani Alain Berberian Christophe Riedel Catherine Duport Fr édéric Carlin Source Type: research
Synergistic antifungal mechanism of cinnamaldehyde and nonanal against Aspergillus flavus and its application in food preservation
Food Microbiol. 2024 Aug;121:104524. doi: 10.1016/j.fm.2024.104524. Epub 2024 Mar 25.ABSTRACTAspergillus flavus colonization on agricultural products during preharvest and postharvest results in tremendous economic losses. Inspired by the synergistic antifungal effects of essential oils, the aims of this study were to explore the mechanism of combined cinnamaldehyde and nonanal (SCAN) against A. flavus and to evaluate the antifungal activity of SCAN loading into diatomite (DM). Shriveled mycelia were observed by scanning electron microscopy, especially in the SCAN treatment group. Calcofluor white staining, transmission el...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Wei Zhang Cuixiang Li Yangyong Lv Shan Wei Yuansen Hu Source Type: research
Fungicidal efficiency of DBD cold plasma against Aspergillus niger on dried jujube
This study investigated the fungicidal efficiency and mechanism of action of dielectric barrier discharge cold atmosphere plasma (DBD-CAP) in inactivating Aspergillus niger (A. niger) spores. The disinfection efficacy and quality of dried jujube used as the processing application object were also studied. The results indicated that the Weibull + Tail model performed better for spore inactivation curves at different voltages among various treatment times, and the spore cells were reduced by 4.05 log (cfu/mL) in spores suspension at 70 kV after 15 min of treatment. This disinfection impact was further supported by scanning e...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Qiaoyun Wei Yuan Yuan Jianhao Zhang Jin Wang Source Type: research
