Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
Inhibition of choline kinase as an antiamoebic approach in Entamoeba histolytica infection
This study investigates a novel anti-amoebic approach targeting the CDP-choline pathway. Inhibition studies were conducted using potential choline kinase (CK) inhibitors to inhibit the EhCK enzyme, and RNA interference was employed to knock down the EhCK gene. Km and Vmax of purified EhCK and hCKa2 proteins were determined by pyruvate kinase-lactate dehydrogenase (PK-LDH) coupled assay. The IC50 values for EhCK and hCKa2 were determined with several commercial CK inhibitors. Selected inhibitors were incubated with E. histolytica trophozoites for 48 hours to determine the EC50 for each inhibitor. Silencing of gene encoding ...
Source: Tropical Biomedicine - February 3, 2024 Category: Tropical Medicine Authors: Z H Teh B H Lim W C See Too L L Few Source Type: research
Genes, Vol. 15, Pages 201: Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens
In conclusion, our findings suggest that E. invadens Myb proteins regulate stage-specific proteins and a wide array of cellular processes. This study provides a foundation for further exploration of the molecular mechanisms governing encystation and unveils potential targets for therapeutic intervention in amebiasis. (Source: Genes)
Source: Genes - February 2, 2024 Category: Genetics & Stem Cells Authors: Patricia Cuellar Elizabeth J. Casta ñeda-Ortiz C ésar Rosales-Zarza Carlos E. Mart ínez-Rodríguez Israel Canela-P érez Mario Alberto Rodr íguez Jes ús Valdés Elisa Azuara-Liceaga Tags: Article Source Type: research
Ethnobotanical study of medicinal plants used by the people of Mosop, Nandi County in Kenya
Conclusion: The current study will lay a foundation of knowledge for future research investigations. The abundance of knowledge regarding ethnomedicinal species and their medicinal applications will stimulate further phytochemical and pharmacological research, which could lead to the discovery of potentially significant pharmaceuticals. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - January 19, 2024 Category: Drugs & Pharmacology Source Type: research
A comparison of clinical outcomes associated with dosing metronidazole every 8 hours versus every 12 hours: a systematic review and metaanalysis
CONCLUSIONS: This review suggests that dosing metronidazole every 12 hours is as effective as every-8-hour dosing for hospitalized patients with anaerobic infections. These encouraging findings would benefit from validation by a multicenter randomized controlled trial since there would be many benefits to a 12-hour dosing interval while achieving similar clinical outcomes with traditional dosing. The studies in this systematic review excluded patients with Clostridioides difficile and central nervous system and amebiasis infections, so the findings do not apply to these infection types.PMID:38174024 | PMC:PMC10761045 | DOI...
Source: Baylor University Medical Center Proceedings - January 4, 2024 Category: Universities & Medical Training Authors: Theresa A Jizba Faran Ahmad Ryan W Walters Pamela A Foral Christopher J Destache Manasa Velagapudi Source Type: research
A comparison of clinical outcomes associated with dosing metronidazole every 8 hours versus every 12 hours: a systematic review and metaanalysis
CONCLUSIONS: This review suggests that dosing metronidazole every 12 hours is as effective as every-8-hour dosing for hospitalized patients with anaerobic infections. These encouraging findings would benefit from validation by a multicenter randomized controlled trial since there would be many benefits to a 12-hour dosing interval while achieving similar clinical outcomes with traditional dosing. The studies in this systematic review excluded patients with Clostridioides difficile and central nervous system and amebiasis infections, so the findings do not apply to these infection types.PMID:38174024 | PMC:PMC10761045 | DOI...
Source: Baylor University Medical Center Proceedings - January 4, 2024 Category: Universities & Medical Training Authors: Theresa A Jizba Faran Ahmad Ryan W Walters Pamela A Foral Christopher J Destache Manasa Velagapudi Source Type: research
A comparison of clinical outcomes associated with dosing metronidazole every 8 hours versus every 12 hours: a systematic review and metaanalysis
CONCLUSIONS: This review suggests that dosing metronidazole every 12 hours is as effective as every-8-hour dosing for hospitalized patients with anaerobic infections. These encouraging findings would benefit from validation by a multicenter randomized controlled trial since there would be many benefits to a 12-hour dosing interval while achieving similar clinical outcomes with traditional dosing. The studies in this systematic review excluded patients with Clostridioides difficile and central nervous system and amebiasis infections, so the findings do not apply to these infection types.PMID:38174024 | PMC:PMC10761045 | DOI...
Source: Baylor University Medical Center Proceedings - January 4, 2024 Category: Universities & Medical Training Authors: Theresa A Jizba Faran Ahmad Ryan W Walters Pamela A Foral Christopher J Destache Manasa Velagapudi Source Type: research
A comparison of clinical outcomes associated with dosing metronidazole every 8 hours versus every 12 hours: a systematic review and metaanalysis
CONCLUSIONS: This review suggests that dosing metronidazole every 12 hours is as effective as every-8-hour dosing for hospitalized patients with anaerobic infections. These encouraging findings would benefit from validation by a multicenter randomized controlled trial since there would be many benefits to a 12-hour dosing interval while achieving similar clinical outcomes with traditional dosing. The studies in this systematic review excluded patients with Clostridioides difficile and central nervous system and amebiasis infections, so the findings do not apply to these infection types.PMID:38174024 | PMC:PMC10761045 | DOI...
Source: Baylor University Medical Center Proceedings - January 4, 2024 Category: Universities & Medical Training Authors: Theresa A Jizba Faran Ahmad Ryan W Walters Pamela A Foral Christopher J Destache Manasa Velagapudi Source Type: research
Testosterone affects type I/type II interferon response of neutrophils during hepatic amebiasis
Differences in immune response between men and women may influence the outcome of infectious diseases. Intestinal infection with Entamoeba histolytica leads to hepatic amebiasis, which is more common in males. Previously, we reported that innate immune cells contribute to liver damage in males in the murine model for hepatic amebiasis. Here, we focused on the influences of sex and androgens on neutrophils in particular. Infection associated with neutrophil accumulation in the liver was higher in male than in female mice and further increased after testosterone treatment in both sexes. Compared with female neutrophils, male...
Source: Frontiers in Immunology - December 21, 2023 Category: Allergy & Immunology Source Type: research
Unusual pediatric lung infections: imaging findings
AbstractPediatric lung infections continue to be a leading cause of pediatric morbidity and mortality. Although both pediatric and general radiologists are familiar with typical lung infections and their imaging findings in children, relatively rare lung infections continue to present a diagnostic challenge. In addition, the advances in radiological imaging and emergence of several new lung infections in recent years facilitated the need for up-to-date knowledge on this topic. In this review article, we discuss the imaging findings of pediatric lung infections caused by unusual/uncommon and new pathogens. We review the epi...
Source: Pediatric Radiology - December 14, 2023 Category: Radiology Source Type: research
Effects of Serum Antibody Test Reagent Discontinuation on Diagnosis of Amebiasis in Japan: Interrupted Time-Series Analysis
This study aimed to investigate changes occurring after discontinuation of the serum antibody test reagent production. We retrospectively analyzed amebiasis cases from January 2014 to December 2019 using the National Center for Epidemiology of Infectious Diseases system. Interrupted time-series regression analysis was used to evaluate trends in weekly amebiasis cases before and after the discontinuation period. The study period was divided into prediscontinuation (2014-2017) and discontinuation (2018-2019) periods. A total of 6,179 amebiasis cases were reported. The average numbers of weekly cases were 21.5 during 2014-201...
Source: The American Journal of Tropical Medicine and Hygiene - December 11, 2023 Category: Tropical Medicine Authors: Yoshiro Hadano Hirotake Mori Satoshi Oono Source Type: research
Effects of Serum Antibody Test Reagent Discontinuation on Diagnosis of Amebiasis in Japan: Interrupted Time-Series Analysis
This study aimed to investigate changes occurring after discontinuation of the serum antibody test reagent production. We retrospectively analyzed amebiasis cases from January 2014 to December 2019 using the National Center for Epidemiology of Infectious Diseases system. Interrupted time-series regression analysis was used to evaluate trends in weekly amebiasis cases before and after the discontinuation period. The study period was divided into prediscontinuation (2014-2017) and discontinuation (2018-2019) periods. A total of 6,179 amebiasis cases were reported. The average numbers of weekly cases were 21.5 during 2014-201...
Source: Am J Trop Med Hyg - December 11, 2023 Category: Infectious Diseases Authors: Yoshiro Hadano Hirotake Mori Satoshi Oono Source Type: research
Effects of Serum Antibody Test Reagent Discontinuation on Diagnosis of Amebiasis in Japan: Interrupted Time-Series Analysis
This study aimed to investigate changes occurring after discontinuation of the serum antibody test reagent production. We retrospectively analyzed amebiasis cases from January 2014 to December 2019 using the National Center for Epidemiology of Infectious Diseases system. Interrupted time-series regression analysis was used to evaluate trends in weekly amebiasis cases before and after the discontinuation period. The study period was divided into prediscontinuation (2014-2017) and discontinuation (2018-2019) periods. A total of 6,179 amebiasis cases were reported. The average numbers of weekly cases were 21.5 during 2014-201...
Source: The American Journal of Tropical Medicine and Hygiene - December 11, 2023 Category: Tropical Medicine Authors: Yoshiro Hadano Hirotake Mori Satoshi Oono Source Type: research
