Fumagillin inhibits growth of the enteric protozoan parasite < em > Entamoeba histolytica < /em > by covalently binding to and selectively inhibiting methionine aminopeptidase 2
In this report, we showed that fumagillin covalently binds to methionine aminopeptidase 2 (MetAP2) and non-covalently but abundantly binds to patatin family phospholipase A (PLA). Susceptibility against fumagillin of the amebic strains in which expression of E. histolytica MetAP2 (EhMetAP2) gene was silenced increased compared to control strain. Conversely, overexpression of EhMetAP2 mutants that harbors amino acid substitutions responsible for resistance to ovalicin, a fumagillin analog, in human MetAP2, also resulted in decrease in fumagillin susceptibility. In contrast, neither gene silencing nor overexpression of E. hi...
Source: Antimicrobial Agents and Chemotherapy - October 24, 2023 Category: Microbiology Authors: Natsuki Watanabe Yumiko Saito-Nakano Naoaki Kurisawa Keisuke Otomo Kiyotake Suenaga Kentaro Nakano Tomoyoshi Nozaki Source Type: research
Fumagillin inhibits growth of the enteric protozoan parasite < em > Entamoeba histolytica < /em > by covalently binding to and selectively inhibiting methionine aminopeptidase 2
In this report, we showed that fumagillin covalently binds to methionine aminopeptidase 2 (MetAP2) and non-covalently but abundantly binds to patatin family phospholipase A (PLA). Susceptibility against fumagillin of the amebic strains in which expression of E. histolytica MetAP2 (EhMetAP2) gene was silenced increased compared to control strain. Conversely, overexpression of EhMetAP2 mutants that harbors amino acid substitutions responsible for resistance to ovalicin, a fumagillin analog, in human MetAP2, also resulted in decrease in fumagillin susceptibility. In contrast, neither gene silencing nor overexpression of E. hi...
Source: Antimicrobial Agents and Chemotherapy - October 24, 2023 Category: Microbiology Authors: Natsuki Watanabe Yumiko Saito-Nakano Naoaki Kurisawa Keisuke Otomo Kiyotake Suenaga Kentaro Nakano Tomoyoshi Nozaki Source Type: research
On the occurrence of a glutaredoxin-like small protein in the anaerobic protozoan parasite Entamoeba histolytica
CONCLUSIONS AND GENERAL SIGNIFICANCE: Our findings support the existence of a new metabolic actor in this pathogen. To the best of our knowledge, this is the first report on this protein class in E. histolytica.PMID:37827204 | DOI:10.1016/j.bbagen.2023.130489 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - October 12, 2023 Category: Biochemistry Authors: Franco Birocco Lihue N Gonzalez Sergio A Guerrero Alberto A Iglesias Diego G Arias Source Type: research
On the occurrence of a glutaredoxin-like small protein in the anaerobic protozoan parasite Entamoeba histolytica
CONCLUSIONS AND GENERAL SIGNIFICANCE: Our findings support the existence of a new metabolic actor in this pathogen. To the best of our knowledge, this is the first report on this protein class in E. histolytica.PMID:37827204 | DOI:10.1016/j.bbagen.2023.130489 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - October 12, 2023 Category: Biochemistry Authors: Franco Birocco Lihue N Gonzalez Sergio A Guerrero Alberto A Iglesias Diego G Arias Source Type: research
Sex Difference in Amebiasis
Curr Top Microbiol Immunol. 2023;441:209-224. doi: 10.1007/978-3-031-35139-6_8.ABSTRACTInfection with the protozoan parasite Entamoeba histolytica is much more likely to cause severe, focal liver damage in males than females, although the infection rate is the same in both sexes. The differences in disease susceptibility may be due to modulation of key mechanisms of the innate immune response by sex hormones. Complement-mediated mechanisms and estrogen-dependent activated natural killer T cells lead to early elimination of the parasite in females, whereas a pathological immune axis is triggered in males. Testosterone, whic...
Source: Current Topics in Microbiology and Immunology - September 11, 2023 Category: Microbiology Authors: Marco Er-Lukowiak Charlotte Hansen Hanna Lotter Source Type: research
