Induction of the ER stress response in NRVMs is linked to cardiotoxicity caused by celastrol
In this study, we explore celastrol's cardiotoxicity using the methods of histology and cell biology. The results show that celastrol administration dose-dependently induces cardiac dysfunction in mice as manifested by left ventricular dilation, myocardial interstitial fibrosis, and cardiomyocyte hypertrophy. Exposure to celastrol greatly decreases neonatal rat ventricular myocyte (NRVM) viability and promotes its apoptosis. More importantly, we demonstrate that celastrol exerts its pro-apoptotic effects through endoplasmic reticulum (ER) stress and unfolded protein response. Furthermore, siRNA targeting C/EBP homologous p...
Source: Acta Biochimica et Biophysica Sinica - August 19, 2022 Category: Biochemistry Authors: Zhong Chen Zhong Zhuang Chen Meng Zhonghua Zhu Yin Zhang Zhao Zhang Source Type: research
Induction of the ER stress response in NRVMs is linked to cardiotoxicity caused by celastrol
In this study, we explore celastrol's cardiotoxicity using the methods of histology and cell biology. The results show that celastrol administration dose-dependently induces cardiac dysfunction in mice as manifested by left ventricular dilation, myocardial interstitial fibrosis, and cardiomyocyte hypertrophy. Exposure to celastrol greatly decreases neonatal rat ventricular myocyte (NRVM) viability and promotes its apoptosis. More importantly, we demonstrate that celastrol exerts its pro-apoptotic effects through endoplasmic reticulum (ER) stress and unfolded protein response. Furthermore, siRNA targeting C/EBP homologous p...
Source: Acta Biochimica et Biophysica Sinica - August 19, 2022 Category: Biochemistry Authors: Zhong Chen Zhong Zhuang Chen Meng Zhonghua Zhu Yin Zhang Zhao Zhang Source Type: research
Impacts of Low-Dose Total Glycosides of Tripterygium wilfordii plus Methotrexate on Immunological Function and Inflammation Level in Patients with Rheumatoid Arthritis
CONCLUSIONS: LD-total GTW plus MTX is highly effective and safe in enhancing the immunity, lowering the inflammation level, and improving the joint function of RA patients.PMID:35959351 | PMC:PMC9359844 | DOI:10.1155/2022/7523673 (Source: Computational and Mathematical Methods in Medicine)
Source: Computational and Mathematical Methods in Medicine - August 12, 2022 Category: Statistics Authors: Yong Han Jingri Jin Fushun Wu Zhiwei Wang Source Type: research
Acylated sucroses and butenolide analog from the leaves of Tripterygium wilfordii Hook. f. and their potential anti-tyrosinase effects
Fitoterapia. 2022 Jul 4:105250. doi: 10.1016/j.fitote.2022.105250. Online ahead of print.ABSTRACTThree undescribed acylated sucroses (1-3), one undescribed butenolide analog (4) along with three known compounds (5-7) were isolated from the aqueous EtOH extract of the dried leaves of Tripterygium wilfordii. Their structures were elucidated on the basis of spectroscopic analyses, electron circular dichroism (ECD) techniques, and saccharide hydrolysis. All the isolated compounds were tested for their anti-tyrosinase effects. Among them, 6 exhibited similar inhibitory effects on tyrosinase with IC50 values of 0.073 mM comparin...
Source: Fitoterapia - July 7, 2022 Category: Biochemistry Authors: Yun-Fei Ai Shu-Hui Dong Bin Lin Xiao-Xiao Huang Shao-Jiang Song Source Type: research
Acylated sucroses and butenolide analog from the leaves of Tripterygium wilfordii Hook. f. and their potential anti-tyrosinase effects
Fitoterapia. 2022 Jul 4;161:105250. doi: 10.1016/j.fitote.2022.105250. Online ahead of print.ABSTRACTThree undescribed acylated sucroses (1-3), one undescribed butenolide analog (4) along with three known compounds (5-7) were isolated from the aqueous EtOH extract of the dried leaves of Tripterygium wilfordii. Their structures were elucidated on the basis of spectroscopic analyses, electron circular dichroism (ECD) techniques, and saccharide hydrolysis. All the isolated compounds were tested for their anti-tyrosinase effects. Among them, 6 exhibited similar inhibitory effects on tyrosinase with IC50 values of 0.073 mM comp...
Source: Fitoterapia - July 7, 2022 Category: Biochemistry Authors: Yun-Fei Ai Shu-Hui Dong Bin Lin Xiao-Xiao Huang Shao-Jiang Song Source Type: research
Qingluo Tongbi Formula Alleviates Hepatotoxicity Induced by Tripterygium wilfordii Hook. F. by Regulating Excessive Mitophagy Through the PERK-ATF4 Pathway
In conclusion, QTF can alleviate TW-induced hepatotoxicity by differentially downregulating the PERK-ATF4 pathway and excessive mitophagy by different components. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - July 7, 2022 Category: Drugs & Pharmacology Source Type: research
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling
CONCLUSION: Overall, our results indicated that TP attenuated LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via repression of the mTOR/STAT3 signaling. Therefore, TP might be an effective agent for IBD treatment.PMID:35793125 | DOI:10.1080/08923973.2022.2093738 (Source: Immunopharmacology and Immunotoxicology)
Source: Immunopharmacology and Immunotoxicology - July 6, 2022 Category: Allergy & Immunology Authors: Huanhuan Zhu Shaopeng Tong Congrong Yan Acheng Zhou Minying Wang Chunwei Li Source Type: research
Therapeutic effect of (5R)-5-hydroxytriptolide (LLDT-8) in SIV infected rhesus monkeys
CONCLUSIONS: LLDT-8 administration during chronic SIV infection had no effect on T cell activation and plasma levels; Furthermore, LLDT-8 may not contribute to suppression of viral rebound and reservoir. These results suggest that LLDT-8 is unlikely to reduce immune activation and viral persistence without additional interventions.PMID:35716483 | DOI:10.1016/j.intimp.2022.108932 (Source: International Immunopharmacology)
Source: International Immunopharmacology - June 18, 2022 Category: Allergy & Immunology Authors: Tingxia Lv Wei Cao Jing Xue Qiang Wei Zhifeng Qiu Yang Han Taisheng Li Source Type: research
Therapeutic Potential of Triptolide in Treating Bone-Related Disorders
Triptolide, a diterpene triepoxide, is a pharmacologically active compound isolated from a Chinese medicinal herb Tripterygium wilfordii Hook F (TwHF). Triptolide has attracted considerable attention in recent times due to its multiple biological and pharmaceutical activities, with an emphasis on therapeutic importance in the treatment of diverse disorders. With essential medicinal implications, TwHF’s extracts have been used as anti-inflammatory, antiproliferative, antioxidative, and immunosuppressive agents for centuries, with continuous and relevant modifications to date to enhance its utility in several diseases and ...
Source: Frontiers in Pharmacology - June 15, 2022 Category: Drugs & Pharmacology Source Type: research
New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway
In this study, we show that triptolide activated the cGAS-STING signaling pathway in kidney tubular cells in vivo and in vitro. Renal injury models were established in BALB/c mice and human tubular epithelial cells using TPL. We found that TPL enhanced the phosphorylation levels of STING, TBK1 and IRF3, and upregulated the expression of IFNβ, which is the production of cGAS-STING signaling pathway. STING inhibitor C176 had protective effects in TPL-induced nephrocyte damage. STING siRNA down regulated the expression level of IFNβ. In addition, triptolide induced an increase in protein levels of the transcription factor B...
Source: Free Radical Biology and Medicine - June 13, 2022 Category: Biology Authors: Jun Lu Yi Zhang Huiyue Dong Jingjing Sun Ling Zhu Pengyang Liu Fuli Wen Rong Lin Source Type: research
