New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway
In this study, we show that triptolide activated the cGAS-STING signaling pathway in kidney tubular cells in vivo and in vitro. Renal injury models were established in BALB/c mice and human tubular epithelial cells using TPL. We found that TPL enhanced the phosphorylation levels of STING, TBK1 and IRF3, and upregulated the expression of IFNβ, which is the production of cGAS-STING signaling pathway. STING inhibitor C176 had protective effects in TPL-induced nephrocyte damage. STING siRNA down regulated the expression level of IFNβ. In addition, triptolide induced an increase in protein levels of the transcription factor B...
Source: Free Radical Biology and Medicine - June 13, 2022 Category: Biology Authors: Jun Lu Yi Zhang Huiyue Dong Jingjing Sun Ling Zhu Pengyang Liu Fuli Wen Rong Lin Source Type: research
New mechanism of nephrotoxicity of triptolide: Oxidative stress promotes cGAS-STING signaling pathway
In this study, we show that triptolide activated the cGAS-STING signaling pathway in kidney tubular cells in vivo and in vitro. Renal injury models were established in BALB/c mice and human tubular epithelial cells using TPL. We found that TPL enhanced the phosphorylation levels of STING, TBK1 and IRF3, and upregulated the expression of IFNβ, which is the production of cGAS-STING signaling pathway. STING inhibitor C176 had protective effects in TPL-induced nephrocyte damage. STING siRNA down regulated the expression level of IFNβ. In addition, triptolide induced an increase in protein levels of the transcription factor B...
Source: Free Radical Biology and Medicine - June 13, 2022 Category: Biology Authors: Jun Lu Yi Zhang Huiyue Dong Jingjing Sun Ling Zhu Pengyang Liu Fuli Wen Rong Lin Source Type: research
Celastrol alleviates high-fat diet-induced obesity via enhanced muscle glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of pyruvate dehydrogenase complex
In conclusion, the current findings accentuate the therapeutic potential of celastrol against HFD-induced obese mice via enhanced glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of PDC activity in the skeletal muscle.PMID:35679966 | DOI:10.1016/j.taap.2022.116099 (Source: Toxicology and Applied Pharmacology)
Source: Toxicology and Applied Pharmacology - June 9, 2022 Category: Toxicology Authors: Mohamad Hafizi Abu Bakar Nor Shafiqah Nor Shahril Mohamad Shamil Faris Mohamad Khalid Sharifah Mohammad Khairul Anuar Shariff Thiruventhan Karunakaran Rabeta Mohd Salleh Mohamad NorishamMohamad Rosdi Source Type: research
Celastrol alleviates high-fat diet-induced obesity via enhanced muscle glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of pyruvate dehydrogenase complex
In conclusion, the current findings accentuate the therapeutic potential of celastrol against HFD-induced obese mice via enhanced glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of PDC activity in the skeletal muscle.PMID:35679966 | DOI:10.1016/j.taap.2022.116099 (Source: Toxicology and Applied Pharmacology)
Source: Toxicology and Applied Pharmacology - June 9, 2022 Category: Toxicology Authors: Mohamad Hafizi Abu Bakar Nor Shafiqah Nor Shahril Mohamad Shamil Faris Mohamad Khalid Sharifah Mohammad Khairul Anuar Shariff Thiruventhan Karunakaran Rabeta Mohd Salleh Mohamad NorishamMohamad Rosdi Source Type: research
Triptolide inhibits T-cell acute lymphoblastic leukaemia by affecting aberrant epigenetic events in the Wnt signalling pathway
In conclusion, our results suggest that TPL inhibits T-ALL by inhibiting aberrant epigenetic events in dysregulated Wnt signalling.PMID:35666085 | DOI:10.1080/1120009X.2022.2082347 (Source: Journal of Chemotherapy)
Source: Journal of Chemotherapy - June 6, 2022 Category: Cancer & Oncology Authors: Yanna Ma Ying Li Mei Huang Yuesheng Meng Source Type: research
A Bibliometric Analysis of Triptolide and the Recent Advances in Treating Non –Small Cell Lung Cancer
In this study, 1,106 Web-of-Science-indexed manuscripts and 1,160 Chinese-National-Knowledge-Infrastructure-indexed manuscripts regarding triptolide published between 2011 and 2021 were analyzed, mapping the co-occurrence networks of keywords and clusters using CiteSpace software. The research frontier and development trend were determined by keyword frequency and cluster analysis, which can be used to predict the future research development of triptolide. Non–small cell lung cancer (NSCLC) is most common in lung cancer patients, accounting for about 80% of all lung cancer patients. New evidence suggests that triptolide ...
Source: Frontiers in Pharmacology - May 30, 2022 Category: Drugs & Pharmacology Source Type: research
Recent Advances in Clinical Diagnosis and Pharmacotherapy Options of Membranous Nephropathy
Membranous nephropathy (MN) is the most common cause of nephrotic syndrome among adults, which is the leading glomerular disease that recurs after kidney transplantation. Treatment for MN remained controversial and challenging, partly owing to absence of sensitive and specific biomarkers and effective therapy for prediction and diagnosis of disease activity. MN starts with the formation and deposition of circulating immune complexes on the outer area in the glomerular basement membrane, leading to complement activation. The identification of autoantibodies against the phospholipase A2 receptor (PLA2R) and thrombospondin ty...
Source: Frontiers in Pharmacology - May 26, 2022 Category: Drugs & Pharmacology Source Type: research
Native Endophytes of Tripterygium wilfordii-Mediated Biotransformation Reduces Toxicity of Celastrol
This study describes a method of microbial transformation to increase the modification site of celastrol and reduce its toxicity. The screening of endophytes from native plants was introduced in this context, which led to two novel stereoselective oxidation products such as S-16-hydroxyl celastrol (2) and A-ring aromatized S-16-hydroxyl celastrol (3), along with a rare 7,9-octadecadienoic acid ester of celastrol (4). Their structures were determined by extensive spectroscopic data analysis, especially 1D and 2D NMR. Compared with 1, compounds 3 and 4 exhibited similar antitumor activity in U251, A549, KG-1, and B16 cell li...
Source: Frontiers in Microbiology - May 25, 2022 Category: Microbiology Source Type: research
