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Effect of dihydroarteminin combined with siRNA targeting Notch1 on Notch1/c-Myc signaling in T-cell lymphoma cells.
Authors: Huo L, Wei W, Wu S, Zhao X, Zhao C, Zhao H, Sun L Abstract The effectiveness of therapy combining dihydroartemisinin (DHA) and small interfering RNA targeting Notch1 (siNotch1) in T-cell lymphoma remains unknown. The present study explored the potential and possible mechanisms of combined dihydroarteminin, and siNotch1 therapy for T-cell lymphoma. It was demonstrated that the viability rates of siRNA-DHA-treated cells was significantly suppressed in comparison with those in control cells, control siRNA cells, siRNA-treated cells and DHA-treated cells (P<0.01). Additionally, there was a significant incre...
Source: Experimental and Therapeutic Medicine - March 31, 2018 Category: General Medicine Tags: Exp Ther Med Source Type: research

Evaluation of siRNA and Cationic Liposomes Complexes as a Model for in vitro siRNA Delivery to Cancer Cells
This study aims to investigate the effect of different molar charge ratios (R+/-) between positive charges from microfluidics-produced cationic liposomes (CL) (egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and negative charges from siRNA and on physico-chemical and morphological properties of the lipoplexes (CL/siRNA) as well as their in vitro luciferase silencing effect in HeLa cells. R+/- 3.27 was found to be the optimum point for complexation. This finding was confirmed by gel retardation and siRNA accessibility assays. According to Cryo-TEM analysis...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - July 6, 2018 Category: Chemistry Source Type: research

Additive polyplexes to undertake siRNA therapy against CDC20 and survivin in breast cancer cells.
Abstract Small interfering RNA (siRNA) delivered to silence overexpressed genes associated with malignancies is a promising targeted therapy to decrease the uncontrolled growth of malignant cells. To create potent delivery agents for siRNA, here we formulated additive polyplexes of siRNA using linoleic acid-substituted polyethylenimine and additive polymers (hyaluronic acid, polyacrylic acid, dextran sulfate and methyl cellulose), and characterized their physicochemical properties and effectiveness. Incorporating polyanionic polymer along with anionic siRNA in polyplexes was found to decrease the ζ-potential of p...
Source: Biomacromolecules - September 17, 2018 Category: Biochemistry Authors: Parmar MB, K C RB, Löbenberg R, Uludag H Tags: Biomacromolecules Source Type: research

Oral siRNA Delivery Using Dual Transporting Systems to Efficiently Treat Colorectal Liver Metastasis
Publication date: Available online 3 November 2018Source: International Journal of PharmaceuticsAuthor(s): Eun-Ju Hyun, Mohammad Nazmul Hasan, Sung Hun Kang, Sungpil Cho, Yong-Kyu LeeAbstractOral siRNA delivery is an ideal way to translate siRNA therapeutic effects in the clinic due to its ability to be administered in convenient and multiple dosages. However, an effective oral delivery system requires overcoming both a hostile gastrointestinal (GI) environment and non-specific targeting. Here, an HTsRP-NC system is a new oral siRNA delivery system consisting of a siRNA/protamine (sRP) nano-complex protected by a multi-fun...
Source: International Journal of Pharmaceutics - November 4, 2018 Category: Drugs & Pharmacology Source Type: research

Worm ‐Like Biomimetic Nanoerythrocyte Carrying siRNA for Melanoma Gene Therapy
This study reports a third type of nanoworm, biomimetic nanoerythrocytes for siRNA delivery, except for filomicelle and nanoworm iron ‐oxide particle, which is the first approach that allows for targeted siRNA delivery by a process involving red blood cell (RBC) membrane cloaking of charge‐reversible polyplexes of siRNA and polycation. RBC membrane cloaking protects siRNA from RNase A degradation. Moreover, the RBC membrane‐ cloaked charge‐reversible siRNA vector (RBC‐reversible polyplex (RP)) not only stays longer in the blood circulation than that of negatively charged bovine serum albumin (BSA) spheres and pos...
Source: Small - October 17, 2018 Category: Nanotechnology Authors: Yanming Wang, Xin Ji, Miaoliang Ruan, Wen Liu, Rongguang Song, Jian Dai, Wei Xue Tags: Full Paper Source Type: research

siRNA-mediated protein knockdown in precision-cut lung slices.
Abstract Small interfering RNA (siRNA) can induce RNA interference, which leads to the knockdown of messenger RNA (mRNA) and protein. As a result, siRNA is often used in vitro and in vivo to unravel the function of genes and as a therapeutic agent to disrupt excessive expression of disease-related genes. However, there is a large gap between in vitro and in vivo models in terms of simplicity, flexibility, throughput, and translatability. This gap could be bridged by using precision-cut tissue slices, which represent viable explants prepared from animal or human tissue that can be cultured ex vivo. Previously, we d...
Source: European Journal of Pharmaceutics and Biopharmaceutics - November 7, 2018 Category: Drugs & Pharmacology Authors: Ruigrok MJR, Xian JL, Frijlink HW, Melgert BN, Hinrichs WLJ, Olinga P Tags: Eur J Pharm Biopharm Source Type: research

Let-7 miRNA and CDK4 siRNA co-encapsulated in Herceptin-conjugated liposome for breast cancer stem cells
In conclusion, an efficient liposomal delivery system for the combination of miRNA and siRNA to target the BCSCs was developed and could be used as an efficacious therapeutic modality for breast cancer.Graphical AbstractHerceptin-conjugated cationic liposome can enhance the cellular uptake of let-7 miRNA and CDK4 siRNA toward the HER-2 expressing breast cancer stem cells.
Source: Asian Journal of Pharmaceutical Sciences - May 8, 2019 Category: Drugs & Pharmacology Source Type: research

Effect of thermal and shear stresses in the spray drying process on the stability of siRNA dry powders
In conclusion, stable siRNA based particles for inhalation purposes could be produced using the spray drying technology.Graphical abstract
Source: International Journal of Pharmaceutics - May 9, 2019 Category: Drugs & Pharmacology Source Type: research

Delivery of siRNA using folate receptor-targeted pH-sensitive polymeric nanoparticles for rheumatoid arthritis therapy
Publication date: Available online 22 May 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Xiangshi Sun, Shiyan Dong, Xiangyu Li, Kongtong Yu, Fengying Sun, Robert J. Lee, Youxin Li, Lesheng TengAbstractSystemic delivery of siRNA to target tissues is difficult to achieve owing to its limited cellular uptake and poor serum stability. Herein, polymeric nanoparticles were developed for systemic administration of siRNA to inflamed tissues. The polymeric nanoparticles were composed of PK3 as a pH-sensitive polymer, folate-polyethyleneglycol-poly(lactide-co-glycolide) as a targeting ligand, and a DOTAP/si...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - May 23, 2019 Category: Nanotechnology Source Type: research

Molecules, Vol. 24, Pages 2211: Current Aspects of siRNA Bioconjugate for In Vitro and In Vivo Delivery
i Studies on siRNA delivery have seen intense growth in the past decades since siRNA has emerged as a new class of gene therapeutics for the treatment of various diseases. siRNA bioconjugate, as one of the major delivery strategies, offers the potential to enhance and broaden pharmacological properties of siRNA, while minimizing the heterogeneity and stability-correlated toxicology. This review summarizes the recent developments of siRNA bioconjugate, including the conjugation with antibody, peptide, aptamer, small chemical, lipidoid, cell-penetrating peptide polymer, and nanoparticle. These siRNA bioconjugate, either ...
Source: Molecules - June 12, 2019 Category: Chemistry Authors: Wanyi Tai Tags: Review Source Type: research

Therapeutic effects of human monoclonal PSMA antibody-mediated TRIM24 siRNA delivery in PSMA-positive castration-resistant prostate cancer
Conclusion: PSMAb mediated TRIM24 siRNA delivery platform could significantly inhibit cell proliferation, colony-formation, and invasion in PSMA+ CRPC in vitro and suppressed tumor growth and bone loss in PSMA+ CRPC xenograft and bone metastasis model.
Source: Theranostics - June 13, 2019 Category: Molecular Biology Authors: Sheng-Jia Shi, Li-Juan Wang, Dong-Hui Han, Jie-Heng Wu, Dian Jiao, Kai-Liang Zhang, Jiang-Wei Chen, Yu Li, Fa Yang, Jing-Liang Zhang, Guo-Xu Zheng, An-Gang Yang, Ai-Zhi Zhao, Wei-Jun Qin, Wei-Hong Wen Tags: Research Paper Source Type: research

Key determinants of siRNA delivery mediated by unique pH-responsive lipid-based liposomes
Publication date: Available online 12 August 2019Source: International Journal of PharmaceuticsAuthor(s): Mariko Sako, Furan Song, Ayaka Okamoto, Hiroyuki Koide, Takehisa Dewa, Naoto Oku, Tomohiro AsaiAbstractLipid-based nanoparticles, a potential nonviral vector due to their good biocompatibility and biodegradability, have been extensively developed for the delivery of small interfering RNA (siRNA). We designed a unique pH-responsive lipid derivative, a dioleylphosphate-diethylenetriamine conjugate (DOP-DETA). DOP-DETA consists of a pH-responsive triamine and unsaturated fatty acids that accelerate membrane fusion. Our re...
Source: International Journal of Pharmaceutics - August 13, 2019 Category: Drugs & Pharmacology Source Type: research

Transmigration of Tetraspanin 2 (Tspan2) siRNA Via Microglia Derived Exosomes across the Blood Brain Barrier Modifies the Production of Immune Mediators by Microglia Cells
AbstractMicroglia are implicated in the neuropathogenesis of HIV. Tetraspanin 2 (Tspan2) is closely related to CD9 and CD81 proteins, and are expressed on microglia cells. They have been implicated in cell fusion and adhesion and in the immune response, and neuroinflammation. Developing therapeutics that target microglia remains a challenge as these therapeutics must cross the Blood-Brain Barrier (BBB). Our goal was to use microglia derived exosomes as a vehicle to deliver siRNA across the BBB to target human telomerase reverse transcriptase immortalized human microglial cells (HTHU) latently infected by HIV (HTHU-HIV) and...
Source: Journal of NeuroImmune Pharmacology - December 9, 2019 Category: Drugs & Pharmacology Source Type: research

Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment
AbstractMetastasis is one of the main reasons causing death in cancer patients. It was reported that chemotherapy might induce metastasis. In order to uncover the mechanism of chemotherapy-induced metastasis and find solutions to inhibit treatment-induced metastasis, the relationship between epithelial-mesenchymal transition (EMT) and doxorubicin (DOX) treatment was investigated and a redox-sensitive small interfering RNA (siRNA) delivery system was designed. DOX-related reactive oxygen species (ROS) were found to be responsible for the invasiveness of tumor cells in vitro, causing enhanced EMT and cytoskeleton reconstruct...
Source: Journal of Zhejiang University. Science. B. - February 29, 2020 Category: Universities & Medical Training Source Type: research