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Total 40893 results found since Jan 2013.
siRNA-Mediated SBF2 Silencing May Inhibit Pancreatic Cancer Cells via Attenuation of the TGF-β Signaling Pathway.
CONCLUSION: SiRNA-mediated SBF2 silencing could significantly inhibit the proliferation and promote the apoptosis of pancreatic cancer cells possibly via attenuation of transforming growth factor β/SMAD signaling pathway. And SBF2 silencing can be a potential approach for treatment of pancreatic cancer.
PMID: 25882882 [PubMed - as supplied by publisher]
Source: Technology in Cancer Research and Treatment - April 16, 2015 Category: Cancer & Oncology Authors: Long J, Liu Z, Wu X, Xu Y, Ge C Tags: Technol Cancer Res Treat Source Type: research
One-step scalable preparation method for non-cationic liposomes with high siRNA content
Publication date: 25 July 2015 Source:International Journal of Pharmaceutics, Volume 490, Issues 1–2 Author(s): Masaharu Somiya , Kotomi Yamaguchi , Qiushi Liu , Tomoaki Niimi , Andrés Daniel Maturana , Masumi Iijima , Nobuo Yoshimoto , Shun’ichi Kuroda Cationic liposomes (LPs) have been utilized for short interfering RNA (siRNA) delivery in vitro and in vivo owing to their high affinity for siRNA via electrostatic binding. However, both cytotoxicity and non-specific adsorption of cationic LPs in the body have prevented clinical siRNA applications. These situations have led to siRNA encapsulation in non-cationic LPs...
Source: International Journal of Pharmaceutics - June 8, 2015 Category: Drugs & Pharmacology Source Type: research
Inhalation Properties and Stability of Nebulized Naked siRNA Solution for Pulmonary Therapy.
Abstract
The use of naked unmodified small interfering RNA (N-siRNA) without vector has previously been investigated as a pulmonary therapy. However, little is known regarding stabilities and aerodynamic particle sizes of N-siRNA-containing droplets; nebulizers have not yet been optimized for N-siRNA solutions. Thus, in this study, we investigated the feasibility of inhaled N-siRNA solutions for pulmonary therapy using nebulization. Various nebulizers and N-siRNA concentrations were assessed in terms of siRNA integrity after nebulization, and inhalation properties including aerodynamic particle size were examined....
Source: Chemical and Pharmaceutical Bulletin - January 13, 2016 Category: Drugs & Pharmacology Authors: Tahara K, Hashimoto W, Takeuchi H Tags: Chem Pharm Bull (Tokyo) Source Type: research
Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tumor-specific therapy
This study demonstrates that the constructed vesicle in combination with hyperthermia could greatly improve the in vivo stability of siRNA-CPPs and synergistically enhance its cancer therapy efficiency. Graphical abstract
Source: International Journal of Pharmaceutics - April 27, 2016 Category: Drugs & Pharmacology Source Type: research
Utility of the 2-Nitrobenzenesulfonamide Group as a Chemical Linker for Enhanced Extracellular Stability and Cytosolic Cleavage in siRNA-Conjugated Polymer Systems.
Abstract
Herein we report the 2-nitrobenzenesulfonamide group as a new chemical linker that responds to the difference in redox potential across the cellular membrane, toward the construction of siRNA-polymer conjugates. PEG-conjugated to siRNA via the 2-nitrobenzenesulfonamide group (PEG-sul-siRNA) exhibited highly selective siRNA release under intracellular conditions due to the exclusive presence of GSH/GST ratios in the cell. In addition, siRNA release from PEG-sul-siRNA under extracellular reductive conditions was dramatically suppressed relative to PEG-siRNA conjugates containing a conventional redox-sensiti...
Source: ChemMedChem - November 9, 2016 Category: Chemistry Authors: Huang CH, Takemoto H, Nomoto T, Tomoda K, Matsui M, Nishiyama N Tags: ChemMedChem Source Type: research
Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research
Molecules, Vol. 23, Pages 1586: The Mechanism for siRNA Transmembrane Assisted by PMAL
nnan Lu
The capacity of silencing genes makes small interfering RNA (siRNA) appealing for curing fatal diseases. However, the naked siRNA is vulnerable to and degraded by endogenous enzymes and is too large and too negatively charged to cross cellular membranes. An effective siRNA carrier, PMAL (poly(maleic anhydride-alt-1-decene) substituted with 3-(dimethylamino) propylamine), has been demonstrated to be able to assist siRNA transmembrane by both experiments and molecular simulation. In the present work, the mechanism of siRNA transmembrane assisted by PMAL was studied using steered molecular dynamics simulations bas...
Source: Molecules - June 29, 2018 Category: Chemistry Authors: Yanfei Lu Jipeng Li Nan Su Diannan Lu Tags: Article Source Type: research
High Fat and Diet Induced Obesity
i-FectTM Delivers Again!Research shows that rats and humans on a high-fat diet (HFD) are less sensitive to satiety signals known to act via vagal afferent pathways. Impaired vagal afferent responsiveness to both gastric satiety hormones (CCK and leptin) and mechanical stimulation raises the possibility that changes in electrophysiological properties may be the underlying mechanism responsible for impaired vagal responsiveness to a wide variety of satiety signals.Potassium channels play a central role. To demonstrate this researchers used ouri-Fect siRNA Transfection Kit to silence TRESK and TASK1 to understand there impact...
Source: Neuromics - September 16, 2019 Category: Neuroscience Tags: diabetes iFect obesity Obesity Research RNAi siRNA siRNA delivery in-vivo Source Type: news
Treatment of acute lung inflammation by pulmonary delivery of anti-TNF- α siRNA with PAMAM dendrimers in a murine model.
In this study, commercially available generation 3 poly(amidoamine) (PAMAM) dendrimer was investigated for pulmonary delivery of siRNA directed against tumor necrosis factor (TNF) α for the treatment of acute lung inflammation. Delivery efficiency was assessed in vitro in the RAW264.7 macrophage cell line activated with lipopolysaccharide (LPS), and efficacy was evaluated in vivo in a murine model of LPS-induced lung inflammation upon pre-treatment with TNF-α siRNA. The PAMAM dendrimer-siRNA complexes (dendriplexes) displayed strong siRNA condensation and high cellular uptake in macrophages compared with non-complexed si...
Source: European Journal of Pharmaceutics and Biopharmaceutics - August 12, 2020 Category: Drugs & Pharmacology Authors: Bohr A, Tsapis N, Foged C, Andreana I, Yang M, Fattal E Tags: Eur J Pharm Biopharm Source Type: research
Effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution in mice.
Effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution in mice.
Mol Med Rep. 2020 Nov;22(5):4183-4196
Authors: Hattori Y, Tamaki K, Sakasai S, Ozaki KI, Onishi H
Abstract
Polyethylene glycol (PEG)‑modifications (PEGylations) of cationic liposome/small interfering RNA complexes (siRNA lipoplexes) can enhance their systemic stability. The present study determined the effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution after intravenous injection. Three types of dialkyl or trial...
Source: Molecular Medicine Reports - October 3, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
PD-1 siRNA-Encapsulated Solid Lipid Nanoparticles Downregulate PD-1 Expression by Macrophages and Inhibit Tumor Growth : PD-1 siRNA-Encapsulated Solid Lipid Nanoparticles.
In conclusion, the PD-1 siRNA-SLNs inhibited tumor growth, likely related to their ability to downregulate PD-1 expression by tumor-associated macrophage (TAMs).
PMID: 33517490 [PubMed - in process]
Source: AAPS PharmSciTech - January 31, 2021 Category: Drugs & Pharmacology Authors: Hanafy MS, Hufnagel S, Trementozzi AN, Sakran W, Stachowiak JC, Koleng JJ, Cui Z Tags: AAPS PharmSciTech Source Type: research
PAMAM versus PEI complexation for siRNA delivery: interaction with model lipid membranes and cellular uptake
ConclusionComplexation of PAMAM dendrimer or bPEI with siRNA modified physicochemical properties of the parent polymer, however it did not impact on the mechanism of interaction with model lipid bilayers or how the polymer/siRNA complex interacted and was internalized by HeLa cells. Interaction of siRNA polymer complexes with cells is related to the action of the parent polymer.Graphical abstract
Source: Pharmaceutical Research - March 22, 2022 Category: Drugs & Pharmacology Source Type: research
Self-crosslinked human serum albumin nanocarriers for systemic delivery of polymerized siRNA to tumors.
In this study, we utilized human serum albumin (HSA), which is the most abundant of the plasma proteins, as a siRNA carrier for systemic tumor-targeted siRNA delivery. Both HSA and siRNA molecules were thiol-introduced to improve the binding affinity for each other. The resulting thiolated HSA (tHSA) and polymerized siRNA (psi) formed stable nanosized complexes (psi-tHSAs) by chemical crosslinking and self-crosslinking. After internalization, the psi-tHSAs showed target gene silencing activity in vitro comparable to conventional Lipofectamine™-siRNA complexes, without remarkable cytotoxicity. After intravenous injection...
Source: Biomaterials - September 16, 2013 Category: Materials Science Authors: Son S, Song S, Lee SJ, Min S, Kim SA, Yhee JY, Huh MS, Chan Kwon I, Jeong SY, Byun Y, Kim SH, Kim K Tags: Biomaterials Source Type: research
