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Effects of particle size and binding affinity for siRNA on the cellular processing, intestinal permeation, and anti‐inflammatory efficacy of polymeric nanoparticles
ConclusionNanoparticles with particle size of 450 nm or more balanced binding affinity for siRNA might be preferable for the treatment of ulcerative colitis. This article is protected by copyright. All rights reserved.
Source: The Journal of Gene Medicine - August 9, 2015 Category: Genetics & Stem Cells Authors: Wenyue Cheng, Cui Tang, Chunhua Yin Tags: Research Article Source Type: research
Cystathionine-gamma-lyase gene silencing with siRNA in monocytes/macrophages protects mice against acute pancreatitis.
Abstract
Hydrogen sulphide (H2S) is an endogenous inflammatory mediator produced by cystathionine-γ-lyase (CSE) in monocytes/macrophages. To determine the role of H2S and macrophages in inflammation, we used small interference RNA (siRNA) to target the CSE gene and investigated its effect in a mouse model of acute pancreatitis. Acute pancreatitis is characterised by increased levels of plasma amylase, myeloperoxidase (MPO) activity and pro-inflammatory cytokines and chemokines in the pancreas and lung. SiRNA treatment attenuated inflammation in the pancreas and lungs of mice following caerulein-induced acute panc...
Source: Applied Microbiology and Biotechnology - September 28, 2015 Category: Microbiology Authors: Badiei A, Chambers ST, Gaddam RR, Fraser R, Bhatia M Tags: Appl Microbiol Biotechnol Source Type: research
Effect of siRNA on Wisp-1 gene expression, proliferation, migration and adhesion of mouse hepatocellular carcinoma cells
Conclusions The inhibition of Wisp-1 expression can reduce the proliferation, migration and adhesion of mouse hepatocellular carcinoma cells, which is related to the AKT/glycogen synthase kinase-3β pathway. Wisp-1 gene may be the potential target to cure the hepatocellular carcinoma.
Source: Asian Pacific Journal of Tropical Medicine - October 4, 2015 Category: Tropical Medicine Source Type: research
Liposomes and Lipid Envelope-Type Systems for Systemic siRNA Delivery.
Abstract
The 'RNA interference' has emerged as a potential therapeutic strategy owing to its high specificity to silent any malfunctioned gene in diseases with genetic background. Currently intravenous delivery of siRNA has been a preferred way of administration due to high access of blood to the organs where direct delivery is not possible. Among non-viral delivery systems enabling systemic delivery of siRNA, liposomes and lipid envelope systems appear to be promising due to their biocompatibility over other systems. However, these systems are still challenged by toxicity issues, instability in blood, non-specifi...
Source: Current Pharmaceutical Design - October 23, 2015 Category: Drugs & Pharmacology Authors: Vhora I, Patil S, Amrutiya J, Misra A Tags: Curr Pharm Des Source Type: research
Inhibition of murine breast cancer growth and metastasis by survivin-targeted siRNA using disulfide cross-linked linear PEI.
In this study, we developed and characterized a hyperbranched disulfide cross-linked PEI (lPEI-SS) based on linear PEI (lPEI) by ring-opening reaction of propylene sulfide. We evaluated the efficiency of lPEI-SS as a siRNA vector in vitro with luciferase reporter gene system, and investigated the anti-tumor efficacy of survivin-targeted siRNA (siRNA(sur)) on 4T1 murine breast cancer model using lPEI-SS synthesized here. Results from cytotoxicity and hemolysis assay proved that lPEI-SS showed favorable cell and blood compatibility. lPEI-SS/siRNA polyplexes prepared under the optimized condition were compact spherical partic...
Source: European Journal of Pharmaceutical Sciences - November 7, 2015 Category: Drugs & Pharmacology Authors: Liu S, Huang W, Jin MJ, Fan B, Xia GM, Gao ZG Tags: Eur J Pharm Sci Source Type: research
Kv Channels and Acute to Chronic Pain Transistion
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. s...
Source: siRNA and DsiRNA Transfection Efficiency - November 16, 2015 Category: Neuroscience Tags: chronic pain Gene Expression Analysis Gene Silencing i-Fect KV Channels KV Receptors kv1.1 Potassium Channel Subunit pain transmission Source Type: news
Effect and mechanism analysis of siRNA in inhibiting VEGF and its anti-angiogenesis effects in human osteosarcoma bearing rats.
CONCLUSIONS: The growth of tumor tissue in osteosarcoma bearing rats is inhibited in Ad-VEGF-siRNA group, Ad-VEGF-siRNA + neoadjuvant chemotherapy group and Ad-VEGF-siRNA + anti-angiogenesis chemotherapy group. The effect in Ad-VEGF-siRNA + neoadjuvant chemotherapy is more significant than simple biological therapy or Ad-VEGF-siRNA + anti-angiogenesis chemotherapy.
PMID: 26636524 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - December 6, 2015 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Folate Receptor Targeted Delivery of siRNA and Paclitaxel to Ovarian Cancer Cells via Folate Conjugated Triblock Co-polymer to Overcome TLR4 Driven Chemotherapy Resistance.
This study demonstrates that PEI-g-PCL-b-PEG-Fol conjugates are a reliable delivery system for siRNA and are able to mediate therapeutic protein knockdown within ovarian cancer cells. Additionally, this study provides further evidence to link TLR4 levels to chemotherapy resistance.
PMID: 26636884 [PubMed - as supplied by publisher]
Source: Biomacromolecules - December 4, 2015 Category: Biochemistry Authors: Jones SK, Lizzio V, Merkel O Tags: Biomacromolecules Source Type: research
Kv Channels and Pain Transmissiom
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. s...
Source: Neuromics - December 9, 2015 Category: Neuroscience Tags: i-Fect KV receptors. i-Fect siRNA delivery in-vivo Source Type: news
Polyelectrolyte complexes of hTERT siRNA and polyethyleneimine: Effect of degree of PEG grafting on biological and cellular activity.
Authors: Safari F, Tamaddon AM, Zarghami N, Abolmali S, Akbarzadeh A
Abstract
Gene silencing by siRNA (short interfering RNA)-targeted human telomerase reverse transcriptase (hTERT) is considered a successful strategy for cancer gene therapy. Polyelectrolyte complexes (PEC) of siRNA and cationic polymers such as polyethyleneimine (PEI) have been widely used for cellular transfection; however, they demonstrate some disadvantages such as cytotoxicity and extracellular matrix restrictions. PEG grafting technology was used in an attempt to improve the biocompatibility of PECs. Considering that this technology may compr...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 12, 2015 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Gene silencing by 2'-O-methyldithiomethyl-modified siRNA, a prodrug-type siRNA responsive to reducing environment.
Abstract
RNAs bearing various 2'-modifications have been synthesized in an effort to improve nuclease resistance. However, the gene silencing activity of small interfering RNAs (siRNAs) has been decreased or sometimes completely suppressed by the chemical modifications. We previously developed a post-synthetic approach for the synthesis of 2'-O-methyldithiomethyl-modified RNA, which can be converted into unmodified RNA under reducing conditions, and named it Reducing-Environment-Dependent Uncatalyzed Chemical Transforming RNA (REDUCT RNA). Here, the gene silencing activity of REDUCT siRNA bearing 2'-O-methyldithio...
Source: Bioorganic and Medicinal Chemistry Letters - December 22, 2015 Category: Chemistry Authors: Ochi Y, Imai M, Nakagawa O, Hayashi J, Wada SI, Urata H Tags: Bioorg Med Chem Lett Source Type: research
Intracellular Trafficking and Exocytosis of a Multi-Component siRNA Nanocomplex
The objective of this study is to study post internalization trafficking of this siRNA nanocomplex and its multiple components like siRNA, protamine, and streptavidin, in HSCs. After internalization, the nanocomplex entrapped in early endosomes undergoes three possible routes including endosomal escape, exocytosis, and entrapment in lysosomes. Significant amount of siRNA dissociates form the nanocomplex to exert silencing activity. After escaping from endosomes, protamine dissociates from the nanocomplex and stays inside the cytoplasm. Golgi complex plays an important role in exocytosis of the nanocomplex. We also demonstr...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - March 11, 2016 Category: Nanotechnology Source Type: research
Improved neurite outgrowth on central nervous system myelin substrate by siRNA-mediated knockdown of Nogo receptor.
CONCLUSION: siRNA-mediated knockdown of NgR expression contributes to neurite outgrowth in vitro.
PMID: 27033267 [PubMed - in process]
Source: Chinese Journal of Traumatology - January 31, 2016 Category: Orthopaedics Authors: Ding SH, Bao YH, Shen JH, Gao GY, Pan YH, Luo QZ, Jiang JY Tags: Chin J Traumatol Source Type: research
Cytosolic mRNA Target and Bioavailability of Nanoparticulate siRNA delivery systems for gene silencing.
Authors:
Abstract
Recent research in medical and pharmaceutical sciences has benefited from advances in molecular biology and genetics, which made possible a diagnosis at the molecular level in more and more diseases. This implies the drug treatment at the molecular level. The interest in Ribonucleic acid interference (RNAi) is based on the mechanism operates by eliminating the messenger RNAs (mRNAs) coding for multiple proteins, which open solutions for treating many types of diseases. Small (short) interfering RNA (siRNA) has quickly been established as an effective gene-silencing strategy in animal models, and...
Source: Current Drug Targets - June 18, 2016 Category: Drugs & Pharmacology Tags: Curr Drug Targets Source Type: research
The influence of HOXA5-specific siRNA on the expression of Livin and Smac proteins.
CONCLUSIONS: HOXA5-specific siRNA effectively silenced the HOXA5 gene expression and down-regulation of HOXA5 induced the down-regulation of Livin protein expression and up-regulation of Smac protein. We suggest the HOXA5 gene to be considered as the new target for acute leukemia gene therapy.
PMID: 27460741 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - July 29, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
