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Total 40893 results found since Jan 2013.

Oral cancer cells may rewire alternative metabolic pathways to survive from siRNA silencing of metabolic enzymes
Conclusions: Our results indicate that the metabolic defects introduced by siRNA silencing of metabolic enzymes TKT or AK2 may be compensated by alternative feedback metabolic mechanisms, suggesting that cancer cells may overcome single defective pathways through secondary metabolic network adaptations. The highly robust nature of oral cancer cell metabolism implies that a systematic medical approach targeting multiple metabolic pathways may be needed to accomplish the continued improvement of cancer treatment.
Source: BMC Cancer - March 25, 2014 Category: Cancer & Oncology Authors: Min ZhangYang ChaiJeffrey BrumbaughXiaojun LiuRamin RabiiSizhe FengKaori MisunoDiana MessadiShen Hu Source Type: research

Tumor-penetrating codelivery of siRNA and paclitaxel with ultrasound-responsive nanobubbles hetero-assembled from polymeric micelles and liposomes.
Abstract Drug resistance is a big problem in systemic chemotherapy of hepatocellular carcinoma (HCC), and nanomedicines loaded with both chemotherapeutic agents (e.g. paclitaxel, PTX) and siRNA's targeting antiapoptosis genes (e.g. BCL-2) possess the advantages to simultaneously overcome the efflux pump-mediated drug resistance and antiapoptosis-related drug resistance. However, tumor-penetrating drug delivery with this type of nanomedicines is extremely difficult due to their relatively big size compared to the single drug-loaded nanomedicines. Aiming at address this problem, US-responsive nanobubbles encapsulati...
Source: Biomaterials - April 17, 2014 Category: Materials Science Authors: Yin T, Wang P, Li J, Wang Y, Zheng B, Zheng R, Cheng D, Shuai X Tags: Biomaterials Source Type: research

Effects of siRNA targeting BMPR-II on the biological activities of human liver cancer cells and its mechanism
In this study, we observed the effects of small interfering RNA (siRNA) targeting bone morphogenetic protein receptor II (BMPR-II) on the biological activities of human liver cells and explore its mechanism. Methods: The molecular sequences of three siRNA targeting BMPR-IIwere designed and synthesized. In this study, there were 6 groups including group I (normal control), group II (blank control), group III (negative control) and group IV-VI (BMPR-II-siRNA-a, siRNA-b and siRNA-c-transfected cells, respectively). The levels of mRNA and protein of BMPR-II were determined to select the best sequence for BMPR-II silence. After...
Source: Cancer Cell International - June 19, 2014 Category: Cancer & Oncology Authors: Peng ZengSheng CaiJia-na ZhangFeng-ming YiWei-min JiangJian-bing Wu Source Type: research

Matrix metalloproteinase 2-responsive micelle for siRNA delivery.
Abstract Systemic delivery of small interfering RNA (siRNA) into cancer cells remains the major obstacle to siRNA drug development. An ideal siRNA delivery vehicle for systemic administration should have long circulation time in blood, accumulate at tumor site, and sufficiently internalize into cancer cells for high-efficiency of gene silence. Herein, we report a core-shell Micelleplex delivery system that made from block copolymer bearing poly(ethylene glycol) (PEG), matrix metalloproteinase 2 (MMP-2)-degradable peptide PLG*LAG, cationic cell penetrating peptide polyarginine r9 and poly(ε-caprolactone) (PCL) for...
Source: Biomaterials - June 11, 2014 Category: Materials Science Authors: Wang HX, Yang XZ, Sun CY, Mao CQ, Zhu YH, Wang J Tags: Biomaterials Source Type: research

Abstract LB-103: L1CAM-targeted delivery of siRNA using elastin-like polypeptide (ELP) nanoparticles inhibits the growth of human tumors implanted in mice
Small interfering RNA (siRNA) drugs provide ideal means for perturbing intracellular oncogenic targets. However, specific delivery of siRNA to tumors has proven to be difficult. An ELP was engineered with an N-terminal region that binds to L1 cell adhesion molecule (L1CAM), and a C-terminal region that binds siRNA. Upon binding of siRNA, the L1CAM targeted ELP (ELP-L) spontaneously assembled into a spherical nanocomplex with the siRNA protected within, and the CAM-binding region protruding from the surface. These nanoparticles were used to deliver siRNA to SKOV3 tumors formed following surgical implantation of the cells in...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Primiano, T., Chang, B.-D., Heidel, J. D. Tags: Experimental and Molecular Therapeutics Source Type: research

Abstract 4571: Antitumor effects of an antibody (cetuximab)-targeted nanoparticle containing siRNA against EGFR
Conclusions: NPs containing full antibodies as targeting agents and siRNA payloads can be formulated into well defined, stable experimental therapeutics. The NP system used here has a pH-tunable 5-nPBA that allows for targeting and stabilizing the NP at a physiologic pH of 7.4. The targeting agent and PEG coating is able to detach from the NP at acidic pH like those found in endosomes, enabling the siRNA payload to escape and reach its site of action within the cell. These NPs produce significant tumor regression in vivo that is superior to CTX alone. Citation Format: Dorothy W. Pan, Mark E. Davis. Antitumor effects of an ...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Pan, D. W., Davis, M. E. Tags: Experimental and Molecular Therapeutics Source Type: research

Chitosan/siRNA Nanoparticles Targeting Cyclooxygenase Type 2 Attenuate Unilateral Ureteral Obstruction-induced Kidney Injury in Mice
Cyclooxygenase type 2 (COX-2) plays a predominant role in the progression of kidney injury in obstructive nephropathy. The aim of this study was to test the efficacy of chitosan/small interfering RNA (siRNA) nanoparticles to knockdown COX-2 specifically in macrophages to prevent kidney injury induced by unilateral ureteral obstruction (UUO). Using optical imaging techniques and confocal microscopy, we demonstrated that chitosan/siRNA nanoparticles accumulated in macrophages in the obstructed kidney. Consistent with the imaging data, the obstructed kidney contained a higher amount of siRNA and macrophages. Chitosan-formulat...
Source: Theranostics - January 2, 2015 Category: Molecular Biology Authors: Chuanxu Yang, Line Nilsson, Muhammad Umar Cheema, Yan Wang, Jørgen Frøkiær, Shan Gao, Jørgen Kjems, Rikke Nørregaard Tags: Research Paper Source Type: research

Development of hybrid-type modified chitosan derivative nanoparticles for the intracellular delivery of midkine-siRNA in hepatocellular carcinoma cells
Conclusion MixNCH/MK-siRNA nanoparticles could be effective for the treatment of hepatocellular carcinoma.
Source: Hepatobiliary and Pancreatic Diseases International - February 14, 2015 Category: Gastroenterology Source Type: research

A biodegradable stearylated peptide with internal disulfide bonds for efficient delivery of siRNA in vitro and in vivo.
In this study, a stearyl-peptide (SHR) was synthesized using arginine, histidine, cysteine and stearyl moiety. Further, the stearyl-peptides were cross linked by disulfide bonds to obtain cross-linked polypeptides (SHRss) with different molecular weight (SHRss1, SHRss2, SHRss3, SHRss4). The SHRss could effectively condense small interfering RNA (siRNA) into polyplexes with a hydrodynamic size of 100-300 nm and zeta potential of 20-40 mV. Flow cytometry and confocal laser scanning microscope studies revealed high cellular uptake and rapid dissociation behavior of SHRss2/siRNA complexes. Long-lasting high concentration of si...
Source: Biomacromolecules - February 16, 2015 Category: Biochemistry Authors: Tai Z, Wang X, Tian J, Gao Y, Zhang L, Yao C, Wu X, Zhang W, Zhu Q, Gao S Tags: Biomacromolecules Source Type: research

Effects of hydrophobic core components in amphiphilic PDMAEMA nanoparticles on siRNA delivery.
Abstract Due to their biodegradable character, polyesters such as polycaprolactone (PCL), poly(d,l-lactide) (PDLLA), and polylactic-co-glycolic acid (PLGA) were widely used as the hydrophobic cores of amphiphilic cationic nanoparticles (NPs) for siRNA delivery. However, fewer researches focused on facilitating siRNA delivery by adjusting the polyester composition of these nanoparticles. Herein, we investigated the contribution of polyester segments in siRNA delivery in vitro by introducing different ratio of DLLA moieties in PCL segments of mPEG-block-PCL-graft-poly(dimethylamino ethyl methacrylate)(PEG-b-PCL-g-P...
Source: Biomaterials - February 25, 2015 Category: Materials Science Authors: Han S, Cheng Q, Wu Y, Zhou J, Long X, Wei T, Huang Y, Zheng S, Zhang J, Deng L, Wang X, Liang XJ, Cao H, Liang Z, Dong A Tags: Biomaterials Source Type: research

Development of a simple, biocompatible and cost-effective Inulin- Diethylenetriamine based siRNA delivery system.
In conclusion, we developed a novel inulin-based siRNA delivery system able to efficiently release siRNA in JHH6 with negligible cytotoxicity thus opening the way for further testing in more complex in vivo models. PMID: 25845631 [PubMed - as supplied by publisher]
Source: European Journal of Pharmaceutical Sciences - April 3, 2015 Category: Drugs & Pharmacology Authors: Sardo C, Farra R, Licciardi M, Dapas B, Scialabba C, Giammona G, Grassi M, Grassi G, Cavallaro G Tags: Eur J Pharm Sci Source Type: research

Synthesis of a stabilized 177Lu–siRNA complex and evaluation of its stability and RNAi activity
In this study, the effects of PS and 2′-O-Me modifications at the backbone of siRNA on serum stability and RNA interference activity were investigated. Materials and methods: Fully PS and 2′-O-Me-modified type 1 insulin-like growth factor receptor (IGF-1R) siRNA was radiolabeled with lutetium-177 (177Lu) through p-SCN-Bn-DTPA as a chelator. After purification with Vivaspin and PD-10 columns, the radiolabs were examined for stability in serum by instant thin-layer chromatography and polyacrylamide gel electrophoresis. The level of IGF-1R in response to the modified and labeled IGF-1R siRNA was examined using RT-PCR and ...
Source: Nuclear Medicine Communications - April 28, 2015 Category: Nuclear Medicine Tags: Original Articles Source Type: research

Bifunctional siRNA containing immunostimulatory motif 2 enhances protection against pandemic H1N1 virus infection.
CONCLUSIONS: This study paves the way for broad-spectrum RNAi-based therapeutics using immunostimulatory motif towards improved antiviral effect. Hence this approach will be useful to confront the sudden emergence of pandemic strains. PMID: 26264705 [PubMed - as supplied by publisher]
Source: Current Gene Therapy - August 12, 2015 Category: Genetics & Stem Cells Authors: Joshi G, Dash PK, Agarwal A, Sharma S, Parida M Tags: Curr Gene Ther Source Type: research

Development of Guanidinium-rich Protein Mimics for Efficient siRNA Delivery into Human T Cells.
Abstract RNA interference is gaining attention as a means to explore new molecular pathways and for its potential as a therapeutic; however, its application in immortal and primary T cells is limited due to challenges with efficient delivery in these cells types. Herein, we report the development of guanidinium-rich protein transduction domain mimics (PTDMs) based on a ring-opening metathesis polymerization scaffold that delivers siRNA into Jurkat T cells and human peripheral blood mononuclear cells (hPBMCs). Homopolymer and block copolymer PTDMs with varying numbers of guanidinium moieties were designed and teste...
Source: Biomacromolecules - August 31, 2015 Category: Biochemistry Authors: deRonde BM, Torres JA, Minter LM, Tew GN Tags: Biomacromolecules Source Type: research

Role of TRIF Small Interference RNA (siRNA) in Chronic Experimental Allergic Encephalomyelitis (EAE).
CONCLUSIONS The expression of TRIF can be down-regulated by siRNA, thereby alleviating the severity of EAE via its inhibition of interleukin and cytokine release. This may provide new insights for future treatment of MS. PMID: 26324415 [PubMed - in process]
Source: Medical Science Monitor - September 4, 2015 Category: Research Tags: Med Sci Monit Source Type: research