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Total 40893 results found since Jan 2013.

Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA.
In this study, we used 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or lipofectamine to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3-phosphate dehydrogenase (GAPDH); GAPDH expression was then evaluated at the cellular level. In addition, a dermal transport assay was designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier was significantly better than the delivery of siRNA without it. Thus, Our findings identify lipid nanocarriers as excellent candidates for the transdermal d...
Source: Biotechnology Journal - July 16, 2020 Category: Biotechnology Authors: Lee K, Min D, Choi Y, Kim J, Yoon S, Jang J, Park S, Tanaka M, Cho YW, Koo HJ, Jeon H, Choi J Tags: Biotechnol J Source Type: research

Development of a carrier system containing hyaluronic acid and protamine for siRNA delivery in the treatment of melanoma
SummaryThe use of small interfering RNA (siRNA) in melanoma treatment remains limited owing to its biological properties. Herein, we developed a carrier system containing hyaluronic acid and protamine for siRNA delivery. Considering zeta potential and particle size as standards, the ratio of each component in liposome nanoparticles prepared was screened using the control variable method, and siRNA cationic liposome nanoparticles were prepared based on the optimal results obtained. The encapsulation rate of the cationic liposome nanoparticles was measured, and particle morphology was observed. B16F10 cells were treated with...
Source: Investigational New Drugs - August 12, 2020 Category: Drugs & Pharmacology Source Type: research

Effect of pre ‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection.
Effect of pre‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection. Mol Med Rep. 2020 Aug 05;: Authors: Tang M, Hu S, Hattori Y Abstract Our previous study reported that reverse (Rev)‑transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze‑dried in trehalose or sucrose solution resulted in high gene‑silencing activity in cells. The current study investigated whether pre‑freezing or saccharide types present during the freeze‑drying of siRNA lipoplexes affected geneâ€...
Source: Molecular Medicine Reports - September 20, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Lyophilization and nebulization of pulmonary surfactant-coated nanogels for siRNA inhalation therapy.
Abstract RNA interference (RNAi) enables highly specific silencing of potential target genes for treatment of pulmonary pathologies. The intracellular RNAi pathway can be activated by cytosolic delivery of small interfering RNA (siRNA), inducing sequence-specific gene knockdown on the post-transcriptional level. Although siRNA drugs hold many advantages over currently applied therapies, their clinical translation is hampered by inefficient delivery across cellular membranes. We previously developed hybrid nanoparticles consisting of an siRNA-loaded nanosized hydrogel core (nanogel) coated with Curosurf®, a clinic...
Source: European Journal of Pharmaceutics and Biopharmaceutics - October 2, 2020 Category: Drugs & Pharmacology Authors: Merckx P, Lammens J, Nuytten G, Bogaert B, Guagliardo R, Maes T, Vervaet C, De Beer T, De Smedt SC, Raemdonck K Tags: Eur J Pharm Biopharm Source Type: research

Targeted nanomedicine with anti-EGFR scFv for siRNA delivery into triple negative breast cancer cells.
Abstract A targeted nanomedicine with humanized anti-EGFR scFv (NM-scFv) was developed for siRNA delivery into triple negative breast cancer (TNBC) cells. NM-scFv consisted of i) targeted nanovector (NV-scFv): nano-cargo with targeting properties; ii) siRNA: pharmacological agent and iii) cationic polymers (chitosan, poly-L-arginine): for siRNA complexation and endosomal escape. NV-scFv was based on superparamagnetic nanoparticle (SPION) labeled with DylightTM680, a PEG layer and a humanized anti-EGFR scFv. The PEG density was optimized from 236±3 to 873±4 PEGs/NV-scFv and the number of targeting ligands per NV-...
Source: European Journal of Pharmaceutics and Biopharmaceutics - October 12, 2020 Category: Drugs & Pharmacology Authors: Vinh Nguyen P, Hervé-Aubert K, David S, Lautram N, Passirani C, Chourpa I, Aubrey N, Allard-Vannier E Tags: Eur J Pharm Biopharm Source Type: research

Biocompatible AIEgen/p-glycoprotein siRNA@reduction-sensitive paclitaxel polymeric prodrug nanoparticles for overcoming chemotherapy resistance in ovarian cancer
Conclusion: Our findings demonstrated the Py-TPE/siRNA@PMP as a promising agent for the highly efficient treatment of PTX-resistant cells and overcoming the shortage of chemotherapy in ovarian cancer.
Source: Theranostics - April 19, 2021 Category: Molecular Biology Authors: Jun Wu, Quan Wang, Xiaoqi Dong, Min Xu, Juliang Yang, Xiaoqing Yi, Biao Chen, Xiyuan Dong, Ying Wang, Xiaoding Lou, Fan Xia, Shixuan Wang, Jun Dai Tags: Research Paper Source Type: research

Designing an effective therapeutic siRNA to silence RdRp gene of SARS-CoV-2
Infect Genet Evol. 2021 Jun 2:104951. doi: 10.1016/j.meegid.2021.104951. Online ahead of print.ABSTRACTThe devastating outbreak of COVID-19 has spread all over the world and has become a global health concern. There is no specific therapeutics to encounter the COVID-19. Small interfering RNA (siRNA)-based therapy is an efficient strategy to control human viral infections employing post-transcriptional gene silencing (PTGS) through neutralizing target complementary mRNA. RNA-dependent RNA polymerase (RdRp) encoded by the viral RdRp gene as a part of the replication-transcription complex can be adopted as an acceptable targe...
Source: Infection, Genetics and Evolution - June 5, 2021 Category: Genetics & Stem Cells Authors: Mohammad Mahfuz Ali Khan Shawan Ashish Ranjan Sharma Manojit Bhattacharya Bidyut Mallik Farhana Akhter Md Salman Shakil Md Mozammel Hossain Subrata Banik Sang-Soo Lee Md Ashraful Hasan Chiranjib Chakraborty Source Type: research

Genes, Vol. 13, Pages 319: The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects
In this study, we investigated the most responsible region for the off-target effect by conducting a comprehensive analysis of the thermodynamic properties of all possible siRNA subregions that involved a machine learning technique using a random sampling procedure. As a result, the thermodynamic stability of nucleotides 2–5 showed the highest positive correlation with the off-target effect, and nucleotides 8–14 showed the most negative correlation. Thus, it is revealed that the siRNA off-target effect is determined by the base-pairing stabilities of two different subregions with opposite effects.
Source: Genes - February 9, 2022 Category: Genetics & Stem Cells Authors: Yoshiaki Kobayashi Shen Tian Kumiko Ui-Tei Tags: Article Source Type: research

Erratum to: Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment
J Zhejiang Univ Sci B. 2022 Feb 15;23(2):171-172. doi: 10.1631/jzus.B19e0468.ABSTRACTThe online version of the original article can be found at https://doi.org/10.1631/jzus.B1900468 The original version of this article (Liu et al., 2020) unfortunately contained some mistakes. 1. Figs. 7c and 7d in p.229 were incorrect. The upper left and bottom left pictures in Fig. 7c were accidentally duplicated with the pictures at the same position of Fig. 1a. The upper right and bottom right pictures were mistakenly placed in Fig. 7c. Therefore, the calculation results in Fig. 7d were also mistaken. The correct versions should be as f...
Source: J Zhejiang Univ Sci ... - February 21, 2022 Category: Science Authors: Xuan Liu Xueqing Zhou Xuwei Shang Li Wang Yi Li Hong Yuan Fuqiang Hu Source Type: research