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Source: Experimental Cell Research

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Total 221 results found since Jan 2013.

Stromal-derived Factor-1 α signaling is involved in bone morphogenetic protein-2-induced odontogenic differentiation of stem cells from apical papilla via the Smad and Erk signaling pathways.
In conclusion, our results demonstrated that SDF-1α can significantly promote the migration of SCAP. Moreover, we revealed corequirement of the SDF-1α/CXCR4 signaling pathways in the BMP-2-induced odontogenic differentiation of SCAP, and these findings may be applied in new strategies for dental pulp regeneration. PMID: 31071315 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - May 5, 2019 Category: Cytology Authors: Xiao M, Yao B, Zhang BD, Bai Y, Sui W, Wang W, Yu Q Tags: Exp Cell Res Source Type: research

miR3609 sensitizes breast cancer cells to adriamycin by blocking the programmed death-ligand 1 immune checkpoint.
Abstract Chemoresistance is the leading cause of breast cancer therapy failure, and studies of the mechanisms underlying chemoresistance and the treatment of drug-resistant tumors remain challenging. In the present study, we discovered a novel microRNA, miR3609, that influences the malignancy of breast cancer. Our results showed miR-3609 expression was lower in resistant breast cancer cells than in sensitive breast cancer cells (MCF-7), while PD-L1 expression was higher in resistant breast cancer cells than in sensitive breast cancer cells. Co-transfection of a miR-3609 plasmid with a luciferase construct containi...
Source: Experimental Cell Research - March 19, 2019 Category: Cytology Authors: Li D, Wang X, Yang M, Kan Q, Duan Z Tags: Exp Cell Res Source Type: research

Desmocollin 3 has a tumor suppressive activity through inhibition of AKT pathway in colorectal cancer.
In this study, we performed cell-based functional analysis after DSC3 overexpression by stable transfection and knockdown by siRNA in CRC cells. It turned out that overexpression of DSC3 reduced cell proliferation, colony forming ability, induced G0/G1 cell cycle arrest and promoted apoptosis. Further pathway analysis showed that overexpression of DSC3 significantly inhibited the activity of AKT pathway and increased the expression of E-cadherin as well as p53 and p21. In contrast, siRNA-mediated knockdown of DSC3 increased cell proliferation and colony formation, activated the AKT pathway and decreased the expression of E...
Source: Experimental Cell Research - March 7, 2019 Category: Cytology Authors: Cui T, Yang L, Ma Y, Petersen I, Chen Y Tags: Exp Cell Res Source Type: research

miR-194 Inhibits Liver Cancer Stem Cell Expansion by Regulating RAC1 pathway.
Abstract Liver cancer stem cells (CSCs) contribute to tumorigenesis, progression, drug resistance and recurrence of hepatocellular carcinoma (HCC). However, the underlying mechanism for the propagation of liver CSCs remains unclear. Herein, we observed low expression of miR-194 in chemoresistant HCC cells. A remarkable decrease of miR-194 was detected in EpCAM or CD133-positive liver CSCs and CSC-enriched hepatoma spheres. Interference miR-194 facilitated liver CSCs expansion by enhancing the self-renewal of liver CSCs. While up-regulating miR-194 inhibited liver CSCs expansion by suppressing the self-renewal of l...
Source: Experimental Cell Research - March 3, 2019 Category: Cytology Authors: Ran RZ, Chen J, Cui LJ, Lin XL, Fan MM, Cong ZZ, Zhang H, Tan WF, Zhang QG, Zhang YJ Tags: Exp Cell Res Source Type: research

Silencing of Gal-7 inhibits TGF- β1-induced apoptosis of human airway epithelial cells through JNK signaling pathway.
In this study, we investigated the expression and role of Gal-7 in the apoptosis of bronchial epithelial cells BEAS-2B upon TGF-β1 stimulation. TGF-β1 significantly induced apoptosis of BEAS-2B cells, as determined by flow cytometry. Western blot results revealed that the mRNA and protein expression of Gal-7 were obviously increased after TGF-β1 stimulation. Small interfering RNA (siRNA)-mediated knockdown of Gal-7 abrogated TGF-β1-evoked cell apoptosis. Simultaneously, increased Bcl-2 expression, decreased Bax expression and the cleavage of poly ADP-ribose polymerase (PARP) and caspase-3 activity were also monitored i...
Source: Experimental Cell Research - December 27, 2018 Category: Cytology Authors: Sun X, Zhang W Tags: Exp Cell Res Source Type: research

Role of Small Ubiquitin-like Modifier Proteins-1 (SUMO-1) in Regulating Migration and Invasion of Fibroblast-like Synoviocytes from Patients with Rheumatoid Arthritis.
Abstract Rheumatoid arthritis (RA) is featured by erosive cartilage and bone destruction. The enhancing aggressive property of fibroblast-like synoviocytes (FLSs) plays a critical role in this process. Small ubiquitin-like modifier (SUMO) proteins, including SUMO-1, SUMO-2, SUMO-3 and SUMO-4, participate in regulating many cellular events such as survival, migration and signal transduction in some cell lines. However, their roles in the pathogenesis of RA are not well established. Therefore, we evaluated the role of SUMO proteins in RA FLSs migration and invasion. We found that expression of both SUMO-1 and SUMO-2...
Source: Experimental Cell Research - December 15, 2018 Category: Cytology Authors: Lao M, Zhan Z, Li N, Xu S, Shi M, Zou Y, Huang M, Zeng S, Liang L, Xu H Tags: Exp Cell Res Source Type: research

MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.
In conclusion, our study demonstrates that miR-98 targets LASS2 and regulates bladder cancer chemoresistance through modulation of mitochondrial function. PMID: 30463687 [PubMed - in process]
Source: Experimental Cell Research - November 23, 2018 Category: Cytology Authors: Luan T, Fu S, Huang L, Zuo Y, Ding M, Li N, Chen J, Wang H, Wang J Tags: Exp Cell Res Source Type: research

ARHGEF10L Contributes to Liver Tumorigenesis through RhoA-ROCK1 Signaling and the Epithelial-Mesenchymal Transition.
This study used the Illumina GoldenGate microassay, Sequenom MassARRAY and TaqMan to analyze possible correlations between tag single nucleotide polymorphisms (tag SNPs) in the ARHGEF10L locus and various tumor risks. The genotyping analyses demonstrated a strong association of rs2244444 and rs12732894 with liver cancer. Western blotting and immunohistochemistry also revealed increased expression of ARHGEF10L in hepatocellular carcinoma tissues. Furthermore, increased cell proliferation, cell migration and RhoA activity; increased expression of Rho-associated coiled-coil kinase-1 (ROCK1), phospho- Ezrin/Radixin/Moesin (ERM...
Source: Experimental Cell Research - November 13, 2018 Category: Cytology Authors: Tang J, Liu C, Xu B, Wang D, Ma Z, Chang X Tags: Exp Cell Res Source Type: research

Fos-like antigen 2 (FOSL2) promotes metastasis in colon cancer.
Abstract Among different cancers, incidence and mortality of colorectal cancer (CRC) is one of the highest. KRAS mutation is one of the underlying features in the pathogenesis of CRC with CRC tumors harboring mutant KRAS exhibiting a more aggressive behavior compared to CRC tumors with wild type KRAS. We had earlier shown that the microRNA-143 (miR-143) replenishment not only chemosensitizes CRC cell line with mutant KRAS instead of wild-type KRAS gene, to paclitaxel-mediated cytotoxicity, but also inhibits cell migration and invasion ability. Hence, the study aimed to determine how miR-143 replenishment is inhibi...
Source: Experimental Cell Research - August 13, 2018 Category: Cytology Authors: Fei BY, Wang XY, Fang XD Tags: Exp Cell Res Source Type: research

Inhibition of miR-135b by SP-1 promotes hypoxia-induced vascular endothelial cell injury via HIF-1 α.
In conclusion, the SP-1/miR-135b/HIF-1α axis may play a critical role in hypoxia-induced vascular endothelial injury. Our study thus provides novel insights into the role of this transcription factor and miRNAs in the pathogenesis of hypoxia-induced cardiac dysfunctions. PMID: 29883713 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - June 5, 2018 Category: Cytology Authors: Yang S, Yin J, Hou X Tags: Exp Cell Res Source Type: research

A β1-42 Oligomer induces alteration of Tight Junction scaffold proteins via RAGE-mediated Autophagy in bEnd.3 cells.
Aβ1-42 Oligomer induces alteration of Tight Junction scaffold proteins via RAGE-mediated Autophagy in bEnd.3 cells. Exp Cell Res. 2018 May 29;: Authors: Chan Y, Chen W, Wan W, Chen Y, Li Y, Zhang C Abstract Compelling evidences have shown that amyloid-β (Aβ) peptide is one of the major pathogenic factors resulting in blood-brain barrier (BBB) disruption in Alzheimer's disease (AD). However, the mechanism underlying BBB breakdown remains elusive. In our present study, we employed murine brain capillary endothelial cells (bEnd.3) as an in vitro BBB model to investigate the role of autophagy in Aβ1-4...
Source: Experimental Cell Research - May 29, 2018 Category: Cytology Authors: Chan Y, Chen W, Wan W, Chen Y, Li Y, Zhang C Tags: Exp Cell Res Source Type: research

The role and mechanism of KCa3.1 channels in human monocyte migration induced by palmitic acid.
Abstract Monocyte migration into diseased tissues contributes to the pathogenesis of diseases. Intermediate-conductance Ca2+-activated K+ (KCa3.1) channels play an important role in cell migration. However, the role of KCa3.1 channels in mediating monocyte migration induced by palmitic acid (PA) is still unclear. Using cultured THP-1 cells and peripheral blood mononuclear cells from healthy subjects, we investigated the role and signaling mechanisms of KCa3.1 channels in mediating the migration induced by PA. Using methods of Western blotting analysis, RNA interference, cell migration assay and ELISA, we found tha...
Source: Experimental Cell Research - May 21, 2018 Category: Cytology Authors: Ma XZ, Pang ZD, Wang JH, Song Z, Zhao LM, Du XJ, Deng XL Tags: Exp Cell Res Source Type: research

SM22 is required for the maintenance of actin-rich structures generated during bacterial infections.
Abstract The host actin cytoskeleton is utilized by an assortment of pathogenic bacteria to colonize and cause disease in their hosts. Two prominently studied actin-hijacking bacteria are enteropathogenic Escherichia coli (EPEC) and Listeria monocytogenes. EPEC form actin-rich pedestals atop its host cells to move across the intestinal epithelia, while Listeria monocytogenes generate branched actin networks arranged as actin clouds around the bacteria and as comet tails for propulsion within and amongst their host cells. Previous mass spectrometry analysis revealed that a member of the calponin family of actin-bun...
Source: Experimental Cell Research - May 17, 2018 Category: Cytology Authors: Chua MD, Hipolito KJ, Singerr OB, Solway J, Guttman JA Tags: Exp Cell Res Source Type: research

Antihypertensive drug-candesartan attenuates TRAIL resistance in human lung cancer via AMPK-mediated inhibition of autophagy flux.
Abstract Angiotensin II type 1 receptor blockers (ARBs) are widely used as antihypertensive drugs. Candesartan is an ARB that has also been known for its anticancer effects but the exact molecular mechanism is remaining elusive. In this research, we showed for the first time that candesartan treatment significantly sensitized human lung adenocarcinoma cells to Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis by targeting TRAIL-DR5. TRAIL selectively kills cancer cells by binding to death receptors on the cell membrane, beyond the levels causing minimal toxicity in normal cells. Ho...
Source: Experimental Cell Research - April 22, 2018 Category: Cytology Authors: Rasheduzzaman M, Park SY Tags: Exp Cell Res Source Type: research

siRNA - mediated LRP/LR knock-down reduces cellular viability of malignant melanoma cells through the activation of apoptotic caspases.
Abstract The 37kDa/67kDa laminin receptor (LRP/LR) is over-expressed in tumour cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim of the study was to investigate the role of LRP/LR on the cellular viability of early (A375) and late stage (A375SM) malignant melanoma cells. Flow cytometry and western blot analysis revealed that A375SM cells contain more cell-surface and total LRP/LR levels in comparison to the A375 cells,...
Source: Experimental Cell Research - April 10, 2018 Category: Cytology Authors: Rebelo TM, Vania L, Ferreira E, Weiss SFT Tags: Exp Cell Res Source Type: research