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Source: Experimental Cell Research

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Total 221 results found since Jan 2013.

Reciprocal role of SIRT6 and Hexokinase 2 in the regulation of autophagy driven monocyte differentiation.
Abstract Emerging evidences suggest the impact of autophagy on differentiation but the underlying molecular links between metabolic restructuring and autophagy during monocyte differentiation remain elusive. An increase in PPARγ, HK2 and SIRT6 expression was observed upon PMA induced monocyte differentiation. While PPARγ positively regulated HK2 and SIRT6 expression, the latter served as a negative regulator of HK2. Changes in expression of these metabolic modelers were accompanied by decreased glucose uptake and increase in Chibby, a potent antagonist of β-catenin/Wnt pathway. Knockdown of Chibby abrogated PMA...
Source: Experimental Cell Research - September 18, 2017 Category: Cytology Authors: Singh A, Sen E Tags: Exp Cell Res Source Type: research

MicroRNA-140-5p attenuated oxidative stress in Cisplatin induced Acute Kidney Injury by activating Nrf2/ARE pathway through a Keap1-independent mechanism.
Abstract Oxidative stress was predominantly involved in the pathogenesis of acute kidney injury (AKI). Recent studies had reported the protective role of specific microRNAs (miRNAs) against oxidative stress. Hence, we investigated the levels of miR140-5p and its functional role in the pathogenesis of Cisplatin induced AKI. A mice Cisplatin induced-AKI model was established. We found that miR-140-5p expression was markedly increased in mice kidney. Bioinformatics analysis revealed nuclear factor erythroid 2-related factor (Nrf2) was a potential target of miR-140-5p, We demonstrated that miR-140-5p did not affect Ke...
Source: Experimental Cell Research - September 16, 2017 Category: Cytology Authors: Liao W, Fu Z, Zou Y, Wen D, Ma H, Zhou F, Chen Y, Zhang M, Zhang W Tags: Exp Cell Res Source Type: research

Liraglutide, a glucagon-like peptide-1 receptor agonist, facilitates osteogenic proliferation and differentiation in MC3T3-E1 cells through phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), extracellular signal-related kinase (ERK)1/2, and cAMP/protein kinase A (PKA) signaling pathways involving β-catenin.
Abstract Previous studies have proven that Glucagon-like peptide-1 (GLP-1) and its receptor agonist exert favorable anabolic effects on skeletal metabolism. However, whether GLP-1could directly impact osteoblast-mediated bone forming is still controversial, and the underlying molecular mechanism remains to be elucidated. Thus in this paper, we investigated the effects of liraglutide, a glucagon-like peptide-1 (GLP-1) receptor agonist, on murine MC3T3-E1 preosteoblasts proliferation and differentiation and explored the potential cellular basis. Our study confirmed the presence of GLP-1R in MC3T3-E1, and demonstrate...
Source: Experimental Cell Research - September 14, 2017 Category: Cytology Authors: Wu X, Li S, Xue P, Li Y Tags: Exp Cell Res Source Type: research

Knockdown of LRP/LR induces apoptosis in pancreatic cancer and neuroblastoma cells through activation of caspases.
Abstract The 37kDa/67kDa laminin receptor (LRP/LR) serves various physiological and pathological roles such as enhancing tumour-related processes including metastasis, angiogenesis, cellular viability and telomerase activation in cancerous cell lines. The present study investigates the effect of siRNA mediated downregulation of LRP/LR on pancreatic cancer (AsPC-1) and neuroblastoma (IMR-32) cells. MTT and BrdU assays revealed that siRNA mediated downregulation of LRP resulted in a significant reduction in cell viability and cell proliferation. In addition, knock-down of LRP resulted in phosphatidylserine externali...
Source: Experimental Cell Research - September 9, 2017 Category: Cytology Authors: Chetty CJ, Ferreira E, Jovanovic K, Weiss SFT Tags: Exp Cell Res Source Type: research

Cytochrome b561, Copper, β-Cleaved Amyloid Precursor Protein and Niemann-Pick C1 Protein are Involved in Ascorbate-Induced Release and Membrane Penetration of Heparan Sulfate from Endosomal S-Nitrosylated Glypican-1.
Abstract Ascorbate-induced release of heparan sulfate from S-nitrosylated heparan sulfate proteoglycan glypican-1 takes place in endosomes. Heparan sulfate penetrates the membrane and is transported to the nucleus. This process is dependent on copper and on expression and processing of the amyloid precursor protein. It remains unclear how exogenously supplied ascorbate can generate HS-anMan in endosomes and how passage through the membrane is facilitated. Here we have examined wild-type, Alzheimer Tg2576 and amyloid precursor protein (-/-) mouse fibroblasts and human fetal and Niemann-Pick C1 fibroblasts by using ...
Source: Experimental Cell Research - September 9, 2017 Category: Cytology Authors: Cheng F, Fransson LÅ, Mani K Tags: Exp Cell Res Source Type: research

NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis.
In this study, we transfected NLRP3 siRNA into A549 and RLE-6TN cells and treated them with bleomycin (BLM) for 24h. Then, we detected the expression of NLRP3 inflammasome-related proteins, EMT-related proteins and transforming growth factor-β1 (TGF-β1) via western blotting, immunofluorescence and real-time quantitative PCR. The mRNA and protein level of NLRP3, ASC and caspase-1 increased after treatment with BLM. The IL-1β levels were significantly decreased after inhibition of NLRP3 and caspase-1. E-cadherin expression increased and α-SMA was reduced in the BLM group when inhibited by NLRP3. The level of TGF-β1 was ...
Source: Experimental Cell Research - June 4, 2017 Category: Cytology Authors: Tian R, Zhu Y, Yao J, Meng X, Wang J, Xie H, Wang R Tags: Exp Cell Res Source Type: research

SIRT6 inhibits TNF- α-induced inflammation of vascular adventitial fibroblasts through ROS and Akt signaling pathway.
This study aims to investigate the role of SIRT6 in vascular inflammation and it molecular mechanism. We found that tumor necrosis factor-α (TNF-α) did not alter the localization of SIRT6 in vascular adventitial fibroblasts (VAFs), vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). The expression of SIRT1, SIRT6 was decreased in TNF-α-treated VAFs. In contrast, TNF-α significantly increased the expression of monocyte chemotactic protein 1 (MCP-1) and interleukin (IL) -6. Knockdown of SIRT1 and SIRT6 by siRNA significantly enhanced TNF-α-induced expression of MCP-1 and IL-6, respectively. Overe...
Source: Experimental Cell Research - May 4, 2017 Category: Cytology Authors: He Y, Xiao Y, Yang X, Li Y, Wang B, Yao F, Shang C, Jin Z, Wang W, Lin R Tags: Exp Cell Res Source Type: research

Salinomycin acts through reducing AKT-dependent thymidylate synthase expression to enhance erlotinib-induced cytotoxicity in human lung cancer cells.
In this study, we showed that erlotinib (1.25 - 10μM) treatment down-regulating of TS expression in an AKT inactivation manner in two NSCLC cell lines, human lung squamous cell carcinoma H1703 and adenocarcinoma H1975 cells. Knockdown of TS using small interfering RNA (siRNA) or inhibiting AKT activity with PI3K inhibitor LY294002 enhanced the cytotoxicity and cell growth inhibition of erlotinib. A combination of erlotinib and salinomycin resulted in synergistic enhancement of cytotoxicity and cell growth inhibition in NSCLC cells, accompanied with reduced protein levels of phospho-AKT(Ser473), phospho-AKT(Thr308), and TS...
Source: Experimental Cell Research - April 25, 2017 Category: Cytology Authors: Tung CL, Chen JC, Wu CH, Peng YS, Chen WC, Zheng HY, Jian YJ, Wei CL, Cheng YT, Lin YW Tags: Exp Cell Res Source Type: research

Involvement of PUMA in pericyte migration induced by methamphetamine.
This study has demonstrated that methamphetamine-mediated pericytes migration involves PUMA up-regulation. Thus, targeted studies of PUMA could provide insights to facilitate the development of a potential therapeutic approach for alleviation of methamphetamine-induced pericyte migration. PMID: 28408317 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - April 10, 2017 Category: Cytology Authors: Zhang Y, Zhang Y, Bai Y, Chao J, Hu G, Chen X, Yao H Tags: Exp Cell Res Source Type: research

Hedgehog signaling contributes to basic fibroblast growth factor-regulated fibroblast migration.
Abstract Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. Through the RNA-seq analysis, we had identified that the hedgehog (Hh) canonical pathway genes including Smoothened (Smo) and Gli1, were regulated by bFGF. Further analysis revealed that activation of the Hh pathway via up-regulation of Smo promoted fibroblast migration, invasion, and skin wo...
Source: Experimental Cell Research - March 28, 2017 Category: Cytology Authors: Zhu ZX, Sun CC, Ting Zhu Y, Wang Y, Wang T, Chi LS, Cai WH, Zheng JY, Zhou X, Cong WT, Li XK, Jin LT Tags: Exp Cell Res Source Type: research

Integrin signaling potentiates transforming growth factor-beta 1 (TGF- β1) dependent down-regulation of E-Cadherin expression - important implications for Epithelial to Mesenchymal Transition (EMT) in Renal Cell Carcinoma.
Integrin signaling potentiates transforming growth factor-beta 1 (TGF-β1) dependent down-regulation of E-Cadherin expression - important implications for Epithelial to Mesenchymal Transition (EMT) in Renal Cell Carcinoma. Exp Cell Res. 2017 Mar 28;: Authors: Feldkoren B, Hutchinson R, Rapaport Y, Mahajan A, Margulis V Abstract Signal transduction through the transforming growth factor-beta 1 (TGF-β1) pathway affects epithelial to mesenchymal transition (EMT), partly by modulation of E-Cadherin expression. The concurrent impact of extracellular matrix driven regulation of integrin signaling on EMT ha...
Source: Experimental Cell Research - March 28, 2017 Category: Cytology Authors: Feldkoren B, Hutchinson R, Rapaport Y, Mahajan A, Margulis V Tags: Exp Cell Res Source Type: research

NOR1 promotes hepatocellular carcinoma cell proliferation and migration through modulating the Notch signaling pathway.
In conclusion, these results indicate that NOR1 may be involved in the progression of HCC and thus may be a potential target for the treatment of liver cancer. PMID: 28232113 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - February 19, 2017 Category: Cytology Authors: You K, Sun P, Yue Z, Li J, Xiong W, Wang J Tags: Exp Cell Res Source Type: research

Human CLEC16A regulates autophagy through modulating mTOR activity.
Abstract CLEC16A is genetically linked with multiple autoimmune disorders but its functional relevance in autoimmunity remains obscure. Recent evidence has signposted the emerging role of autophagy in autoimmune disease development. Here, by ectopic expression and siRNA silencing, we show that CLEC16A has an inhibitory role in starvation-induced autophagy in human cells. Combining quantitative proteomics and immunoblotting analyses, we found that CLEC16A likely regulates autophagy by activating mTOR pathway. Overexpression of CLEC16A was found to sensitize cells towards the availability of nutrients, resulting in ...
Source: Experimental Cell Research - February 17, 2017 Category: Cytology Authors: Tam RC, Li MW, Gao YP, Pang YT, Yan S, Ge W, Lau CS, Chan VS Tags: Exp Cell Res Source Type: research

Sulfiredoxin involved in the protection of peroxiredoxins against hyperoxidation in the early hyperglycaemia.
Abstract As a direct consequence of hyperglycaemia, the excessive generation of ROS is central to the pathogenesis of diabetic cardiomyopathy. We hypothesize that stimulation of high glucose (HG) results in an increased sulfiredoxin (Srx) expression, which regulates ROS signaling through reducing the hyperoxidized peroxiredoxins (Prxs). We show that hyperoxidized Prxs were initially reduced in the preliminary stage but then dramatically increased in advanced stage and these changes corresponded to a significant increase of Srx expression in the heart of diabetic rats. These time-dependent changes were also confirm...
Source: Experimental Cell Research - February 11, 2017 Category: Cytology Authors: Shi S, Guo Y, Lou Y, Li Q, Cai X, Zhong X, Li H Tags: Exp Cell Res Source Type: research

RELM- β promotes human pulmonary artery smooth muscle cell proliferation via FAK-stimulated surviving.
In conclusion, results suggested that FAK is upstream of survivin in the signaling pathway mediating cell proliferation. FAK seems to be important in RELM-β-induced HPASMC proliferation, partially by upregulating survivin expression. PMID: 28041789 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - December 28, 2016 Category: Cytology Authors: Lin C, Li X, Luo Q, Yang H, Li L, Zhou Q, Li Y, Tang H, Wu L Tags: Exp Cell Res Source Type: research