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Source: Experimental Cell Research

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Total 221 results found since Jan 2013.

Epoxyeicosatrienoic acid prevents maladaptive remodeling in pressure overload by targeting calcineurin/NFAT and Smad-7.
CONCLUSIONS: EET has synergistic actions on cardiomyocytes and cardiac fibroblasts, preventing cardiac hypertrophy through inhibition of Ca2+-mediated calcineurin/NFATc3 signaling cascades, and ameliorating myocardial fibrosis dependent on Smad-7. This work further extends the potential mechanisms of EET, providing a novel therapeutic approach for the treatment of pathological remodeling and heart failure. PMID: 31734152 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - November 13, 2019 Category: Cytology Authors: Li X, Chu G, Zhu F, Zheng Z, Wang X, Zhang G, Wang F Tags: Exp Cell Res Source Type: research

Metformin promotes osteogenic differentiation and protects against oxidative stress-induced damage in periodontal ligament stem cells via activation of the Akt/Nrf2 signaling pathway.
This study found that low concentrations of metformin did not affect cell proliferation but did inhibit adipogenic differentiation and promote osteogenic differentiation of PDLSCs. This positive effect was associated with activation of Akt signaling by metformin. Moreover, applying metformin as either a pretreatment or co-treatment could reduce the amount of reactive oxygen species, enhance antioxidant capacity, and rescue the cell viability and osteogenic differentiation that were negatively affected by H2O2-induced oxidative stress in PDLSCs. In addition, metformin was found to activate the Nrf2 signaling pathway in PDLS...
Source: Experimental Cell Research - November 8, 2019 Category: Cytology Authors: Jia L, Xiong Y, Zhang W, Ma X, Xu X Tags: Exp Cell Res Source Type: research

TWIST1 transcriptionally regulates glycolytic genes to promote the Warburg metabolism in pancreatic cancer.
In this study, we found that the highly conserved transcription factor, TWIST1, is a crucial regulator of aerobic glycolysis in PDAC. Genetic silencing of TWIST1 significantly inhibited the glycolytic phenotypes of PDAC cells as revealed by reduced glucose uptake, lactate production, and extracellular acidification rate, which can be restored by re-expression of siRNA-resistant TWIST1. Moreover, tamoxifen-inducible expression of TWIST1 promoted the Warburg metabolism of PDAC cells. Mechanistically, by luciferase reporter assay and chromatin immunoprecipitation experiment, we showed that TWIST1 can directly increase the exp...
Source: Experimental Cell Research - November 5, 2019 Category: Cytology Authors: Wang XX, Yin GQ, Zhang ZH, Rong ZH, Wang ZY, Du DD, Wang YD, Gao RX, Xian GZ Tags: Exp Cell Res Source Type: research

Kr üppel-like factor 15 is a key suppressor of podocyte fibrosis under rotational force-driven pressure.
Krüppel-like factor 15 is a key suppressor of podocyte fibrosis under rotational force-driven pressure. Exp Cell Res. 2019 Nov 04;:111706 Authors: Yu MY, Kim JE, Lee S, Choi JW, Kim YC, Han SS, Lee H, Cha RH, Lee JP, Lee JW, Kim DK, Kim YS, Yang SH Abstract Krüppel-like factor 15 (KLF15) is a well-known transcription factor associated with podocyte injury and fibrosis. Recently, hypertensive nephropathy was discovered to be closely related to podocyte injury and fibrosis. However, methods to stimulate hypertension in vitro are lacking. Here, we constructed an in vitro model mimicking hypertension us...
Source: Experimental Cell Research - November 3, 2019 Category: Cytology Authors: Yu MY, Kim JE, Lee S, Choi JW, Kim YC, Han SS, Lee H, Cha RH, Lee JP, Lee JW, Kim DK, Kim YS, Yang SH Tags: Exp Cell Res Source Type: research

3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiation.
Abstract Transitioning from a differentiated state to a higher-order of plasticity, by partial rather than full reactivation of pluripotency genes, might be a better approach in regenerative medicine. Hydrogen sulfide plays a crucial role in the maintenance and differentiation of mesenchymal stem cells (MSC) that have the potential to differentiate to a diverse group of mesenchymally derived cells. It was shown that these cells show a heavy reliance on cystathionine-β-synthase (CBS)-derived hydrogen sulfide (H2S) during differentiation. We have found that expression and activity of 3-mercaptopyruvate sulfurtransf...
Source: Experimental Cell Research - October 17, 2019 Category: Cytology Authors: Ostrakhovitch EA, Akakura S, Sanokawa-Akakura R, Tabibzadeh S Tags: Exp Cell Res Source Type: research

Silencing TTK expression inhibits the proliferation and progression of prostate cancer.
CONCLUSION: Our results suggest that TTK is a gene associated with malignancy of PCa and could be a novel therapeutic target for clinical application. PMID: 31605696 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - October 8, 2019 Category: Cytology Authors: Chen S, Wang J, Wang L, Peng H, Xiao L, Li C, Lin D, Yang K Tags: Exp Cell Res Source Type: research

Ubiquitin-specific protease USP36 knockdown impairs Parkin-dependent mitophagy via downregulation of Beclin-1-associated autophagy-related ATG14L.
Abstract Parkin is an ubiquitin ligase regulating mitochondrial quality control reactions, including the autophagic removal of depolarized mitochondria (mitophagy). Parkin-mediated protein ubiquitinations may be counteracted by deubiquitinating enzymes (DUBs). We conducted a high-content imaging screen of Parkin translocation to depolarized mitochondria after siRNA mediated silencing of each DUB in Parkin overexpressing HeLa cells. Knockdown of the ubiquitin-specific protease USP36 led to delayed Parkin translocation while only slightly disturbing the ubiquitination of mitochondrial proteins, but final autophagic ...
Source: Experimental Cell Research - September 20, 2019 Category: Cytology Authors: Geisler S, Jäger L, Golombek S, Nakanishi E, Hans F, Casadei N, Terradas AL, Linnemann C, Kahle PJ Tags: Exp Cell Res Source Type: research

Slit2 signaling contributes to cholestatic fibrosis in mice by activation of hepatic stellate cells.
In conclusion, Slit2 is involved in the activation of HSCs and liver fibrogenesis, highlighting Slit2 as a potential therapeutic target for liver fibrosis. PMID: 31545977 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - September 19, 2019 Category: Cytology Authors: Li C, Yang G, Lin L, Xuan Y, Yan S, Ji X, Song F, Lu M, Lan T Tags: Exp Cell Res Source Type: research

SPOP inhibits mice pancreatic stellate cell activation by promoting FADD degradation in cerulein-induced chronic pancreatitis.
Abstract Pancreatic stellate cells (PSCs) have been recognized as key mediators of pancreatic fibrosis, a characteristic feature of chronic pancreatitis (CP). As a cullin-based E3 ubiquitin ligase, speckle-type POZ protein (SPOP) has been identified to participate in tumorigenesis and organ development. However, its biological role in CP remains unknown. Therefore, this study sought to investigate the changed expression of SPOP in CP and to examine the effect on mice PSCs activation of SPOP. We found that SPOP was downregulated in the pancreatic tissues of cerulein-induced CP mice. siRNA-mediated knockdown of SPOP...
Source: Experimental Cell Research - September 3, 2019 Category: Cytology Authors: Tan P, Wang A, Chen H, Du Y, Qian B, Shi H, Zhang Y, Xia X, Fu W Tags: Exp Cell Res Source Type: research

A study on the involvement of fatty acid synthase in right ventricle dysfunction in pulmonary hypertension.
In conclusion, our study reveals that inhibition of FAS decreases RV hypertrophy and improves cardiac function associated with PH by perking up metabolic functions. PMID: 31465767 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - August 25, 2019 Category: Cytology Authors: Singh N, Shafiq M, Jagavelu K, Hanif K Tags: Exp Cell Res Source Type: research

Reduced exosomal L-Plastin is responsible for radiation-induced bystander effect.
Abstract Radiation-induced bystander effects (RIBE) are discussed as relevant processes during radiotherapy. Irradiated cells are suggested to release growth-inhibitory/DNA-damaging factors transported to non-irradiated cells. However, the molecular nature of this phenomenon has not yet been resolved. We aimed at identifying the growth-inhibitory factor(s) transmitted to non-irradiated cells. RIBE-competent PC3 cells were used to produce conditioned medium (CM) after exposure to ionizing radiation. Indicator cells were incubated with CM and clonogenic survival as well as cell proliferation were determined as endpo...
Source: Experimental Cell Research - July 10, 2019 Category: Cytology Authors: Freudenmann LK, Mayer C, Rodemann HP, Dittmann K Tags: Exp Cell Res Source Type: research

OGDH mediates the inhibition of SIRT5 on cell proliferation and migration of gastric cancer.
Abstract SIRT5 has a wide range of functions in different cellular processes such as glycolysis, TCA cycle and antioxidant defense, which mediates lysine desuccinylation, deglutarylation and demalonylation. Recent evidences have implicated that SIRT5 is a potential suppressor of gastric cancer (GC). However, the underlying mechanism of SIRT5 in gastric cancer is still unclear. Here, we show that SIRT5 expression is significantly decreased in human GC tissues. Functional analysis demonstrates that SIRT5 inhibits cell growth in vitro and in vivo, arrests the cell cycle in G1/S transition, and suppresses migration an...
Source: Experimental Cell Research - June 23, 2019 Category: Cytology Authors: Lu X, Yang P, Zhao X, Jiang M, Hu S, Ouyang Y, Zeng L, Wu J Tags: Exp Cell Res Source Type: research

A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers.
Abstract Small cell lung cancer (SCLC) has been a recalcitrant cancer without significant breakthroughs in clinical treatment during the past three decades. As there is a lack of effective protein inhibitor for SCLC targeted therapy, the discovery of new druggable SCLC biomarkers is a pressing work. Here we identified a new protein biomarker of SCLC, which is high density lipoprotein binding protein (HDLBP), through the aptamer generated by cell-SELEX against SCLC cells. Immunohistochemistry results showed an elevated HDLBP level in SCLC tissues from clinical samples. Attenuating HDLBP expression with siRNA inhibi...
Source: Experimental Cell Research - June 20, 2019 Category: Cytology Authors: Zhou W, Zhao L, Yuan H, Xu L, Tan W, Song Y, Fang X Tags: Exp Cell Res Source Type: research

Resveratrol decreases TNF α-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts.
This study also shows that NF-κB is not the only factor involved in the TNFα-induced ICAM-1 up-regulation and confirms our previous evidence according to which TNFα increases ICAM-1 levels by redox- and non-redox-regulated mechanisms. RE can represent good and useful support in therapies for intestinal inflammatory diseases in which TNFα plays a crucial role in the increase of adhesion molecule expression. PMID: 31233740 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - June 20, 2019 Category: Cytology Authors: Domazetovic V, Bonanomi AG, Stio M, Vincenzini MT, Iantomasi T Tags: Exp Cell Res Source Type: research

Fam208a orchestrates interaction protein network essential for early embryonic development and cell division.
Abstract Maintenance of genome stability is essential for every living cell as genetic information is repeatedly challenged during DNA replication in each cell division event. Errors, defects, delays, and mistakes that arise during mitosis or meiosis lead to an activation of DNA repair processes and in case of their failure, programmed cell death, i.e. apoptosis, could be initiated. Fam208a is a protein whose importance in heterochromatin maintenance has been described recently. In this work, we describe the crucial role of Fam208a in sustaining the genome stability during the cellular division. The targeted deple...
Source: Experimental Cell Research - May 17, 2019 Category: Cytology Authors: Gresakova V, Novosadova V, Prochazkova M, Bhargava S, Jenickova I, Prochazka J, Sedlacek R Tags: Exp Cell Res Source Type: research