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Source: Experimental Cell Research

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Total 221 results found since Jan 2013.

Combination of B7H6-siRNA and temozolomide synergistically reduces stemness and migration properties of glioblastoma cancer cells
This study aimed to understand the potential role and molecular mechanism of the combination therapy of B7H6-siRNA and temozolomide in glioblastoma cancer. U87 cells were treated with B7H6-siRNA and temozolomide, separately and in combination. Cell viability, stemness, cell migration, and apoptosis were measured. The results of this work presented the synergistic effect of B7H6-siRNA and temozolomide in inhibiting the cancerous features of the U87 cell line. Down-regulating B7H6-siRNA expression inhibited the cell viability of U87 glioblastoma cancer cells and increased their sensitivity to temozolomide. In addition, a not...
Source: Experimental Cell Research - May 29, 2023 Category: Cytology Authors: Nadia Allahyarzadeh Khiabani Mohammad Amin Doustvandi Fateme Mohammadnejad Elnaz Salmani Hassan Kohal Neda Boushehri Mahdi Jafarlou Behzad Baradaran Source Type: research

siRNA - mediated LRP/LR knock-down reduces cellular viability of malignant melanoma cells through the activation of apoptotic caspases.
Abstract The 37kDa/67kDa laminin receptor (LRP/LR) is over-expressed in tumour cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim of the study was to investigate the role of LRP/LR on the cellular viability of early (A375) and late stage (A375SM) malignant melanoma cells. Flow cytometry and western blot analysis revealed that A375SM cells contain more cell-surface and total LRP/LR levels in comparison to the A375 cells,...
Source: Experimental Cell Research - April 10, 2018 Category: Cytology Authors: Rebelo TM, Vania L, Ferreira E, Weiss SFT Tags: Exp Cell Res Source Type: research

siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.
In this study, we focused on alanine aminopeptidase (APP) and investigated the function of APP in C2C12 myoblast proliferation and differentiation. In myoblasts and myotubes, APP was mainly localized in the cell membrane as well as expressed at low levels in the cytoplasm and nucleus. The reduction of the APP enzymatic activity impaired the cell cycle progression in C2C12 myoblasts. In addition, apoptosis was induced after APP-knockdown. Finally, myogenic differentiation was also delayed in the APP-suppressed myoblasts. These findings indicate that APP is required for myoblast proliferation and differentiation. PMID: ...
Source: Experimental Cell Research - October 19, 2020 Category: Cytology Authors: Osana S, Kitajima Y, Suzuki N, Xu Y, Murayama K, Nagatomi R Tags: Exp Cell Res Source Type: research

IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells.
In this study, we show that interleukin (IL)-1β induces the expression of MMP-13 and ADAM-28 in homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like (α7(+)hSMSC-OB) cells, and promotes proliferation while inhibiting apoptosis in these cells. At higher concentrations, however, IL-1β failed to induce the expression of these genes and caused an increase in apoptosis. We further employed ADAM-28 small interfering RNA (siRNA) to investigate whether IL-1β-induced MMP-13 expression is linked to this IL-1β-mediated changes in cell proliferation and apoptosis. Silencing ADAM-2...
Source: Experimental Cell Research - March 5, 2014 Category: Cytology Authors: Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M, Nakamura H Tags: Exp Cell Res Source Type: research

Unique proliferation response in odontoblastic cells derived from human skeletal muscle stem cells by cytokine-induced matrix metalloproteinase-3.
Abstract A pro-inflammatory cytokine mixture (CM: interleukin (IL)-1β,tumor necrosis factor-α and interferon-γ) and IL-1β-induced matrix metalloproteinase (MMP)-3 activity have been shown to increase the proliferation of rat dental pulp cells and murine stem cell-derived odontoblast-like cells. This suggests that MMP-3 may regulate wound healing and regeneration in the odontoblast-rich dental pulp. Here, we determined whether these results can be extrapolated to human dental pulp by investigating the effects of CM-induced MMP-3 up-regulation on the proliferation and apoptosis of purified odontoblast-like cells...
Source: Experimental Cell Research - September 22, 2014 Category: Cytology Authors: Ozeki N, Hase N, Kawai R, Yamaguchi H, Hiyama T, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells.
In this study, MMP-3 small interfering RNA (siRNA) was transfected into odontoblast-like cells derived from induced pluripotent stem cells to investigate whether MMP-3 activity is induced by Poly(P) and/or is associated with cell proliferation and differentiation into odontoblast-like cells. Treatment with Poly(P) led to an increase in both cell proliferation and additional odontoblastic differentiation. Poly(P)-treated cells showed a small but significant increase in dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1) mRNA expression, which are markers of mature odontoblasts. The cells also acquired addi...
Source: Experimental Cell Research - February 3, 2015 Category: Cytology Authors: Ozeki N, Hase N, Yamaguchi H, Hiyama T, Kawai R, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Fluid shear stress suppresses TNF-α-induced apoptosis in MC3T3-E1 cells: Involvement of ERK5-AKT-FoxO3a-Bim/FasL signaling pathways.
Abstract TNF-α is known to induce osteoblasts apoptosis, whereas mechanical stimulation has been shown to enhance osteoblast survival. In the present study, we found that mechanical stimulation in the form of fluid shear stress (FSS) suppresses TNF-α induced apoptosis in MC3T3-E1 cells. Extracellular signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family that has been implicated in cell survival. We also demonstrated that FSS imposed by flow chamber in vitro leads to a markedly activation of ERK5, which was shown to be protective against TNF-α-induced apoptosis, where...
Source: Experimental Cell Research - April 5, 2016 Category: Cytology Authors: Bin G, Bo Z, Jing W, Jin J, Xiaoyi T, Cong C, Liping A, Jinglin M, Cuifang W, Yonggang C, Yayi X Tags: Exp Cell Res Source Type: research

Toll-like receptor 4 promotes angiogenesis in pancreatic cancer via PI3K/AKT signaling.
This study aimed to investigate the role of TLR4 in the angiogenesis of pancreatic cancer and the underlying molecular mechanisms. The culture supernatant (conditioned medium) of PANC-1 cells after appropriate treatment was used for the treatment of HUVECs. The proliferation, migration and tube formation of HUVECs were assessed by MTT, Transwell and Matrigel, respectively. In pancreatic cancer tissues, TLR4, VEGF and CD31 were upregulated as determined by immunohistochemistry and the expression of TLR4 and VEGF was positively correlated with microvessel density as detected by CD31 staining. Activation of TLR4 signaling by ...
Source: Experimental Cell Research - July 13, 2016 Category: Cytology Authors: Sun Y, Wu C, Ma J, Yang Y, Man X, Wu H, Li S Tags: Exp Cell Res Source Type: research

RELM- β promotes human pulmonary artery smooth muscle cell proliferation via FAK-stimulated surviving.
In conclusion, results suggested that FAK is upstream of survivin in the signaling pathway mediating cell proliferation. FAK seems to be important in RELM-β-induced HPASMC proliferation, partially by upregulating survivin expression. PMID: 28041789 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - December 28, 2016 Category: Cytology Authors: Lin C, Li X, Luo Q, Yang H, Li L, Zhou Q, Li Y, Tang H, Wu L Tags: Exp Cell Res Source Type: research

Knockdown of LRP/LR induces apoptosis in pancreatic cancer and neuroblastoma cells through activation of caspases.
Abstract The 37kDa/67kDa laminin receptor (LRP/LR) serves various physiological and pathological roles such as enhancing tumour-related processes including metastasis, angiogenesis, cellular viability and telomerase activation in cancerous cell lines. The present study investigates the effect of siRNA mediated downregulation of LRP/LR on pancreatic cancer (AsPC-1) and neuroblastoma (IMR-32) cells. MTT and BrdU assays revealed that siRNA mediated downregulation of LRP resulted in a significant reduction in cell viability and cell proliferation. In addition, knock-down of LRP resulted in phosphatidylserine externali...
Source: Experimental Cell Research - September 9, 2017 Category: Cytology Authors: Chetty CJ, Ferreira E, Jovanovic K, Weiss SFT Tags: Exp Cell Res Source Type: research

Inhibition of miR-135b by SP-1 promotes hypoxia-induced vascular endothelial cell injury via HIF-1 α.
In conclusion, the SP-1/miR-135b/HIF-1α axis may play a critical role in hypoxia-induced vascular endothelial injury. Our study thus provides novel insights into the role of this transcription factor and miRNAs in the pathogenesis of hypoxia-induced cardiac dysfunctions. PMID: 29883713 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - June 5, 2018 Category: Cytology Authors: Yang S, Yin J, Hou X Tags: Exp Cell Res Source Type: research

Silencing of Gal-7 inhibits TGF- β1-induced apoptosis of human airway epithelial cells through JNK signaling pathway.
In this study, we investigated the expression and role of Gal-7 in the apoptosis of bronchial epithelial cells BEAS-2B upon TGF-β1 stimulation. TGF-β1 significantly induced apoptosis of BEAS-2B cells, as determined by flow cytometry. Western blot results revealed that the mRNA and protein expression of Gal-7 were obviously increased after TGF-β1 stimulation. Small interfering RNA (siRNA)-mediated knockdown of Gal-7 abrogated TGF-β1-evoked cell apoptosis. Simultaneously, increased Bcl-2 expression, decreased Bax expression and the cleavage of poly ADP-ribose polymerase (PARP) and caspase-3 activity were also monitored i...
Source: Experimental Cell Research - December 27, 2018 Category: Cytology Authors: Sun X, Zhang W Tags: Exp Cell Res Source Type: research

PKD deletion promotes autophagy and inhibits hypertrophy in cardiomyocyte.
This study, using primary cardiomyocyte culture, PKD knockdown and overexpression, and other molecular techniques, tested our hypothesis that PKD pathway mediates cardiac hypertrophy by negatively regulating autophagy in cardiomyocyte. Neonatal cardiomyocytes were isolated from Wistar rats and cell hypertrophy was induced by norepinephrine treatment (PE, 10-4 mol/L), and divided into the following groups: (1) Vehicle; (2) PE; (3) PE + control siRNA; (4) PE + Rapamycin (100 nM); (5) PE + PKD-siRNA (2 × 108 U/0.1 ml); (6) PE + PKD siRNA + 3 MA (10 mM). The results showed that PE treatment induced cardiomyo...
Source: Experimental Cell Research - November 19, 2019 Category: Cytology Authors: Zhao D, Gao Y, Wang W, Pei H, Xu C, Zhao Z Tags: Exp Cell Res Source Type: research

Long non-coding RNA LUCAT1 regulates the RAS pathway to promote the proliferation and invasion of malignant glioma cells through ABCB1
In this study, the role of lung cancer associated transcript 1 (lncRNA LUCAT 1) in glioma occurrence and development, as well as its possible regulatory mechanism, was explored. We utilized the gene chip technology in the preliminary experiment, and based on the experiment results, selected LUCAT1（NONHSAT102745）, which was significantly upregulated in glioma, and ATP-binding cassette Subfamily B member l (ABCB1), which was significantly down-regulated in co-expression analysis, for study. Next, the expression of LUCAT1 and ABCB1 in cells and tissues was immediately evaluated. Subsequently, the cells were transfected wi...
Source: Experimental Cell Research - October 21, 2022 Category: Cytology Authors: Xia Wu Lvmeng Song Xiangrong Chen Yalan Zhang Shun Li Xiaoping Tang Source Type: research