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Source: Experimental Cell Research
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Total 221 results found since Jan 2013.
TM6SF2 reduces lipid accumulation in vascular smooth muscle cells by inhibiting LOX-1 and CD36 expression
Exp Cell Res. 2023 Jun 2:113666. doi: 10.1016/j.yexcr.2023.113666. Online ahead of print.ABSTRACTTM6SF2, predominantly expressed in the liver and intestine, is closely associated with lipid metabolism. We have demonstrated the presence of TM6SF2 in VSMCs within human atherosclerotic plaques. Subsequent functional studies were conducted to investigate its role in lipid uptake and accumulation in human vascular smooth muscle cells (HAVSMCs) using siRNA knockdown and overexpression techniques. Our results showed that TM6SF2 reduced lipid accumulation in oxLDL-stimulated VSMCs, likely through the regulation of lectin-like oxLD...
Source: Experimental Cell Research - June 4, 2023 Category: Cytology Authors: Ting-Ting Li Yu-Ting Cui Tao-Hua Li Qiong Xiang Yan-Yu Chen Xi-Long Zheng Juan Peng Zhi-Han Tang Source Type: research
Characterization of RACK1-depleted mammalian cells by a palette of microscopy approaches reveals defects in cell cycle progression and polarity establishment
Exp Cell Res. 2023 Jun 30:113695. doi: 10.1016/j.yexcr.2023.113695. Online ahead of print.ABSTRACTThe Receptor for Activated C Kinase 1 (RACK1) is an evolutionarily conserved scaffold protein involved in the regulation of numerous cellular processes. Here, we used CRISPR/Cas9 and siRNA to reduce the expression of RACK1 in Madin-Darby Canine Kidney (MDCK) epithelial cells and Rat2 fibroblasts, respectively. RACK1-depleted cells were examined using coherence-controlled holographic microscopy, immunofluorescence, and electron microscopy. RACK1 depletion resulted in decreased cell proliferation, increased cell area and perimet...
Source: Experimental Cell Research - July 2, 2023 Category: Cytology Authors: Vera Chvalova Vignesh Venkadasubramanian Zuzana Klimova Jana Vojtova Oldrich Benada Ondrej Vanatko Tomas Vomastek Tomas Grousl Source Type: research
NPFF stimulates human ovarian cancer cell invasion by upregulating MMP-9 via ERK1/2 signaling
This study provides evidence that NPFF stimulates EOC cell invasion by upregulating MMP-9 expression through the NPFFR2-mediated ERK1/2 signaling pathway.PMID:37392963 | DOI:10.1016/j.yexcr.2023.113693
Source: Experimental Cell Research - July 1, 2023 Category: Cytology Authors: Ze Wu Qiongqiong Jia Boqun Liu Lanlan Fang Peter C K Leung Jung-Chien Cheng Source Type: research
Characterization of RACK1-depleted mammalian cells by a palette of microscopy approaches reveals defects in cell cycle progression and polarity establishment
Exp Cell Res. 2023 Jun 30:113695. doi: 10.1016/j.yexcr.2023.113695. Online ahead of print.ABSTRACTThe Receptor for Activated C Kinase 1 (RACK1) is an evolutionarily conserved scaffold protein involved in the regulation of numerous cellular processes. Here, we used CRISPR/Cas9 and siRNA to reduce the expression of RACK1 in Madin-Darby Canine Kidney (MDCK) epithelial cells and Rat2 fibroblasts, respectively. RACK1-depleted cells were examined using coherence-controlled holographic microscopy, immunofluorescence, and electron microscopy. RACK1 depletion resulted in decreased cell proliferation, increased cell area and perimet...
Source: Experimental Cell Research - July 2, 2023 Category: Cytology Authors: Vera Chvalova Vignesh Venkadasubramanian Zuzana Klimova Jana Vojtova Oldrich Benada Ondrej Vanatko Tomas Vomastek Tomas Grousl Source Type: research
The combination of osimertinib with Raf inhibitor overcomes osimertinib resistance induced by KRAS amplification in EGFR-mutated lung cancer cells
Exp Cell Res. 2023 Jul 11:113722. doi: 10.1016/j.yexcr.2023.113722. Online ahead of print.ABSTRACTOsimertinib is a third-generation epidermal growth factor receptor (EGFR)1 tyrosine kinase inhibitor (TKI) approved for the treatment of EGFR-positive patients exhibiting a T790 M resistance mutation after treatment with an earlier generation of EGFR TKIs. However, resistance to osimertinib inevitably develops despite its efficacy, and the resistance mechanisms are complex and not fully understood. We established cell lines with acquired resistance to osimertinib from gefitinib- or erlotinib-resistant NSCLC cells using a dose-...
Source: Experimental Cell Research - July 13, 2023 Category: Cytology Authors: Tae-Gul Lee Hye-Min Kang Seo Yun Kim Hye-Ryoun Kim Cheol Hyeon Kim Source Type: research
Lnc-Malat1 promotes slow myofiber-type transformation through sponging miR-129-5p in C2C12 myotubes
In this study, knockdown of lnc-Malat1 by siRNA significantly inhibited the expression of myoblast marker genes (MyHC, MyoD, and MyoG) and slow muscle fiber marker genes (MyHC I), together with repressed expression of mitochondria-related genes COX5A, ACADM, CPTA1, FABP3, and NDUFA1. Overexpression of lnc-Malat1 exerted an opposite effect, promoting myoblast differentiation and slow muscle fiber formation. Dual luciferase reporter assay revealed a direct interaction between lnc-Malat1 and miR-129-5p, and overexpression of lnc-Malat1 significantly inhibited miR-129-5p expression, thereby elevating the expression of Mef2a, m...
Source: Experimental Cell Research - August 27, 2023 Category: Cytology Authors: Yongqi Yue Yuhe Wang Chenglong Wen Yingying Meng Ying Peng Xiao Li Source Type: research
