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Source: Experimental Cell Research
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Total 221 results found since Jan 2013.
Combination of B7H6-siRNA and temozolomide synergistically reduces stemness and migration properties of glioblastoma cancer cells
This study aimed to understand the potential role and molecular mechanism of the combination therapy of B7H6-siRNA and temozolomide in glioblastoma cancer. U87 cells were treated with B7H6-siRNA and temozolomide, separately and in combination. Cell viability, stemness, cell migration, and apoptosis were measured. The results of this work presented the synergistic effect of B7H6-siRNA and temozolomide in inhibiting the cancerous features of the U87 cell line. Down-regulating B7H6-siRNA expression inhibited the cell viability of U87 glioblastoma cancer cells and increased their sensitivity to temozolomide. In addition, a not...
Source: Experimental Cell Research - May 29, 2023 Category: Cytology Authors: Nadia Allahyarzadeh Khiabani Mohammad Amin Doustvandi Fateme Mohammadnejad Elnaz Salmani Hassan Kohal Neda Boushehri Mahdi Jafarlou Behzad Baradaran Source Type: research
USP7 reduction leads to developmental failure of mouse early embryos
Exp Cell Res. 2023 Apr 18;427(2):113605. doi: 10.1016/j.yexcr.2023.113605. Online ahead of print.ABSTRACTAs a member of Ubiquitin-specific protease subfamily, ubiquitin specific protease 7 (USP7) has been reported to participate in a variety of cellular processes, including cell cycle, apoptosis, DNA damage response, and epigenetic modification. However, its function in preimplantation embryos is still obscure. To investigate the functions of USP7 during preimplantation embryo development, we used siRNA to degrade endogenous USP7 messenger RNA. We found that USP7 knockdown significantly decreased the development rate of mo...
Source: Experimental Cell Research - April 20, 2023 Category: Cytology Authors: Xue Zhang Xue-Feng Xie Ang Li Wei Song Chao Li Fei Li Xiao-Zhen Li Xiao-Yan Fan Chang-Yin Zhou Guang Wang Qing-Yuan Sun Xiang-Hong Ou Source Type: research
Dopamine D1 receptor activation ameliorates ox-LDL-induced endothelial cell senescence via CREB/Nrf2 pathway
Exp Cell Res. 2023 Mar 7:113542. doi: 10.1016/j.yexcr.2023.113542. Online ahead of print.ABSTRACTEndothelial cell senescence is involved in endothelial dysfunction and aging-related vascular diseases. The D1-like dopamine receptor (DR1), a number of G-protein-coupled receptors, is currently under consideration as a potential therapeutic target for the prevention of atherosclerosis. However, the role of DR1 in regulating ox-LDL-stimulated endothelial cell senescence remains unknown. Here, we found that the elevated Prx hyperoxidation and reactive oxygen species (ROS) levels in ox-LDL-treated Human umbilical vein endothelial...
Source: Experimental Cell Research - March 9, 2023 Category: Cytology Authors: Yiming Zhao Yuxia Li Hongxia Li Sa Shi Source Type: research
Effects of Semaphorin3A on the growth of sensory and motor neurons
Exp Cell Res. 2023 Feb 8:113506. doi: 10.1016/j.yexcr.2023.113506. Online ahead of print.ABSTRACTAfter peripheral nerve injury, motor and sensory axons can regenerate, but the inaccurate reinnervation of the target leads to poor functional recovery. Schwann cells (SCs) express sensory and motor phenotypes associated with selective regeneration. Semaphorin 3A (Sema3A) is an axonal chemorepellent that plays an essential role in axon growth. SCs can secret Sema3A, and Sema3A presents a different expression pattern at the proximal and distal ends of injured sensory and motor nerves. Hence, in our study, the protein expression ...
Source: Experimental Cell Research - February 10, 2023 Category: Cytology Authors: Mi Shen Chun Zhou Yingchao Tian Tongxin Shang None Qingyun Liang Mengru Ming Fei Ding Yuhua Ji Source Type: research
Silencing FHL2 inhibits bleomycin-induced pulmonary fibrosis through the TGF- β1/Smad signaling pathway
CONCLUSION: Inhibition of FHL2 can effectively reduce the fibrosis process induced by TGF⁃β1 and bleomycin, and then inhibit the fibrosis.PMID:36641135 | DOI:10.1016/j.yexcr.2023.113470
Source: Experimental Cell Research - January 14, 2023 Category: Cytology Authors: Mengkun Shi Huixia Cui Jialun Shi Yunqing Mei Source Type: research
Back to basics: Optimization of DNA and RNA transfer in muscle cells using recent transfection reagents
In conclusion, we reported yet uncharacterized transfection reagents that can serve as a suitable low-cost alternative to the current gold standard in C2C12 cells.PMID:36273532 | DOI:10.1016/j.yexcr.2022.113392
Source: Experimental Cell Research - October 23, 2022 Category: Cytology Authors: Ilaria Cocchiararo M élanie Cornut Hadrien Soldati Alessandro Bonavoglia Perrine Castets Source Type: research
Long non-coding RNA LUCAT1 regulates the RAS pathway to promote the proliferation and invasion of malignant glioma cells through ABCB1
In this study, the role of lung cancer associated transcript 1 (lncRNA LUCAT 1) in glioma occurrence and development, as well as its possible regulatory mechanism, was explored. We utilized the gene chip technology in the preliminary experiment, and based on the experiment results, selected LUCAT1（NONHSAT102745）, which was significantly upregulated in glioma, and ATP-binding cassette Subfamily B member l (ABCB1), which was significantly down-regulated in co-expression analysis, for study. Next, the expression of LUCAT1 and ABCB1 in cells and tissues was immediately evaluated. Subsequently, the cells were transfected wi...
Source: Experimental Cell Research - October 21, 2022 Category: Cytology Authors: Xia Wu Lvmeng Song Xiangrong Chen Yalan Zhang Shun Li Xiaoping Tang Source Type: research
Inhibition of TRPC6 suppressed TGF β-induced fibroblast-myofibroblast transdifferentiation in renal interstitial NRK-49F cells
In conclusion, we demonstrate that TRPC6 is a key regulator of TGF-β1-induced fibroblast-myofibroblast transdifferentiation and provides the mechanism of how TGF-β1 exposure regulates TRPC6 expression in NRK-49F fibroblasts.PMID:36206825 | DOI:10.1016/j.yexcr.2022.113374
Source: Experimental Cell Research - October 7, 2022 Category: Cytology Authors: Shan Jiang Lifei Gu Yixin Hu Younan Ren Zhao Yang Chengzhi Chai Boyang Yu Haitao Ge Zhengyu Cao Fang Zhao Source Type: research
